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Protein

Epithelial cell adhesion molecule

Gene

Epcam

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

ReactomeiR-MMU-202733 Cell surface interactions at the vascular wall

Names & Taxonomyi

Protein namesi
Recommended name:
Epithelial cell adhesion molecule
Short name:
Ep-CAM
Alternative name(s):
Epithelial glycoprotein 314
Short name:
EGP314
Short name:
mEGP314
Protein 289A
Tumor-associated calcium signal transducer 1
CD_antigen: CD326
Gene namesi
Name:Epcam
Synonyms:Tacstd1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:106653 Epcam

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini24 – 266ExtracellularSequence analysisAdd BLAST243
Transmembranei267 – 289HelicalSequence analysisAdd BLAST23
Topological domaini290 – 315CytoplasmicSequence analysisAdd BLAST26

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Tight junction

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000038018324 – 315Epithelial cell adhesion moleculeAdd BLAST292

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi27 ↔ 46PROSITE-ProRule annotation
Disulfide bondi29 ↔ 59PROSITE-ProRule annotation
Disulfide bondi38 ↔ 48PROSITE-ProRule annotation
Disulfide bondi66 ↔ 99PROSITE-ProRule annotation
Disulfide bondi110 ↔ 116PROSITE-ProRule annotation
Glycosylationi111N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi118 ↔ 135PROSITE-ProRule annotation
Glycosylationi198N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

Glycosylation at Asn-198 is crucial for protein stability.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ99JW5
PaxDbiQ99JW5
PeptideAtlasiQ99JW5
PRIDEiQ99JW5

PTM databases

iPTMnetiQ99JW5
PhosphoSitePlusiQ99JW5

Expressioni

Gene expression databases

BgeeiENSMUSG00000045394 Expressed in 247 organ(s), highest expression level in intestine
GenevisibleiQ99JW5 MM

Interactioni

Subunit structurei

Monomer. Interacts with phosphorylated CLDN7 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ99JW5, 3 interactors
STRINGi10090.ENSMUSP00000061935

Structurei

3D structure databases

ProteinModelPortaliQ99JW5
SMRiQ99JW5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini63 – 135Thyroglobulin type-1PROSITE-ProRule annotationAdd BLAST73

Sequence similaritiesi

Belongs to the EPCAM family.Curated

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IFI6 Eukaryota
ENOG4111M3B LUCA
GeneTreeiENSGT00390000018245
HOGENOMiHOG000074086
InParanoidiQ99JW5
KOiK06737
OMAiREKPYDV
OrthoDBiEOG091G0G1E
PhylomeDBiQ99JW5
TreeFamiTF332767

Family and domain databases

CDDicd00191 TY, 1 hit
Gene3Di4.10.800.10, 1 hit
InterProiView protein in InterPro
IPR000716 Thyroglobulin_1
IPR036857 Thyroglobulin_1_sf
PfamiView protein in Pfam
PF00086 Thyroglobulin_1, 1 hit
SMARTiView protein in SMART
SM00211 TY, 1 hit
SUPFAMiSSF57610 SSF57610, 1 hit
PROSITEiView protein in PROSITE
PS00484 THYROGLOBULIN_1_1, 1 hit
PS51162 THYROGLOBULIN_1_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99JW5-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAGPQALAFG LLLAVVTATL AAAQRDCVCD NYKLATSCSL NEYGECQCTS
60 70 80 90 100
YGTQNTVICS KLASKCLAMK AEMTHSKSGR RIKPEGAIQN NDGLYDPDCD
110 120 130 140 150
EQGLFKAKQC NGTATCWCVN TAGVRRTDKD TEITCSERVR TYWIIIELKH
160 170 180 190 200
KERESPYDHQ SLQTALQEAF TSRYKLNQKF IKNIMYENNV ITIDLMQNSS
210 220 230 240 250
QKTQDDVDIA DVAYYFEKDV KGESLFHSSK SMDLRVNGEP LDLDPGQTLI
260 270 280 290 300
YYVDEKAPEF SMQGLTAGII AVIVVVSLAV IAGIVVLVIS TRKKSAKYEK
310
AEIKEMGEIH RELNA
Length:315
Mass (Da):35,019
Last modified:June 1, 2001 - v1
Checksum:iFDA853E165BA8906
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti87Missing in AAA37543 (PubMed:1729376).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M76124 mRNA Translation: AAA37543.1
AK145752 mRNA Translation: BAE26627.1
AK167182 mRNA Translation: BAE39317.1
BC005618 mRNA Translation: AAH05618.1
BC094465 mRNA Translation: AAH94465.1
CCDSiCCDS29018.1
RefSeqiNP_032558.2, NM_008532.2
UniGeneiMm.4259

Genome annotation databases

EnsembliENSMUST00000053577; ENSMUSP00000061935; ENSMUSG00000045394
GeneIDi17075
KEGGimmu:17075
UCSCiuc008duy.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M76124 mRNA Translation: AAA37543.1
AK145752 mRNA Translation: BAE26627.1
AK167182 mRNA Translation: BAE39317.1
BC005618 mRNA Translation: AAH05618.1
BC094465 mRNA Translation: AAH94465.1
CCDSiCCDS29018.1
RefSeqiNP_032558.2, NM_008532.2
UniGeneiMm.4259

3D structure databases

ProteinModelPortaliQ99JW5
SMRiQ99JW5
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ99JW5, 3 interactors
STRINGi10090.ENSMUSP00000061935

PTM databases

iPTMnetiQ99JW5
PhosphoSitePlusiQ99JW5

Proteomic databases

MaxQBiQ99JW5
PaxDbiQ99JW5
PeptideAtlasiQ99JW5
PRIDEiQ99JW5

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000053577; ENSMUSP00000061935; ENSMUSG00000045394
GeneIDi17075
KEGGimmu:17075
UCSCiuc008duy.1 mouse

Organism-specific databases

CTDi4072
MGIiMGI:106653 Epcam

Phylogenomic databases

eggNOGiENOG410IFI6 Eukaryota
ENOG4111M3B LUCA
GeneTreeiENSGT00390000018245
HOGENOMiHOG000074086
InParanoidiQ99JW5
KOiK06737
OMAiREKPYDV
OrthoDBiEOG091G0G1E
PhylomeDBiQ99JW5
TreeFamiTF332767

Enzyme and pathway databases

ReactomeiR-MMU-202733 Cell surface interactions at the vascular wall

Miscellaneous databases

PROiPR:Q99JW5
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000045394 Expressed in 247 organ(s), highest expression level in intestine
GenevisibleiQ99JW5 MM

Family and domain databases

CDDicd00191 TY, 1 hit
Gene3Di4.10.800.10, 1 hit
InterProiView protein in InterPro
IPR000716 Thyroglobulin_1
IPR036857 Thyroglobulin_1_sf
PfamiView protein in Pfam
PF00086 Thyroglobulin_1, 1 hit
SMARTiView protein in SMART
SM00211 TY, 1 hit
SUPFAMiSSF57610 SSF57610, 1 hit
PROSITEiView protein in PROSITE
PS00484 THYROGLOBULIN_1_1, 1 hit
PS51162 THYROGLOBULIN_1_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiEPCAM_MOUSE
AccessioniPrimary (citable) accession number: Q99JW5
Secondary accession number(s): Q61512
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: June 1, 2001
Last modified: November 7, 2018
This is version 123 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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