UniProtKB - Q99JT2 (STK26_MOUSE)
Serine/threonine-protein kinase 26
Stk26
Functioni
Serine/threonine-protein kinase that acts as a mediator of cell growth. Modulates apoptosis. In association with STK24 negatively regulates Golgi reorientation in polarized cell migration upon RHO activation. Phosphorylates ATG4B at 'Ser-383', thereby increasing autophagic flux.
By similarityCatalytic activityi
Cofactori
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 53 | ATPPROSITE-ProRule annotationBy similarity | 1 | |
Active sitei | 144 | Proton acceptorPROSITE-ProRule annotationBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 30 – 38 | ATPPROSITE-ProRule annotationBy similarity | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB
- identical protein binding Source: MGI
- magnesium ion binding Source: UniProtKB
- protein homodimerization activity Source: MGI
- protein kinase activity Source: UniProtKB
- protein serine/threonine/tyrosine kinase activity Source: RHEA
- protein serine/threonine kinase activity Source: UniProtKB-KW
- protein serine kinase activity Source: RHEA
GO - Biological processi
- apoptotic process Source: UniProtKB-KW
- cellular response to starvation Source: UniProtKB
- microvillus assembly Source: MGI
- negative regulation of cell migration Source: UniProtKB
- protein autophosphorylation Source: UniProtKB
- protein phosphorylation Source: UniProtKB
- regulation of apoptotic process Source: UniProtKB
- regulation of hydrogen peroxide-induced cell death Source: MGI
- response to hydrogen peroxide Source: MGI
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Biological process | Apoptosis |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-111465, Apoptotic cleavage of cellular proteins |
Names & Taxonomyi
Protein namesi | Recommended name: Serine/threonine-protein kinase 26Curated (EC:2.7.11.1By similarity)Alternative name(s): Mammalian STE20-like protein kinase 4Curated Short name: MST-4Curated STE20-like kinase MST4Curated Serine/threonine-protein kinase MST4Curated |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1917665, Stk26 |
VEuPathDBi | HostDB:ENSMUSG00000031112 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Golgi apparatus
- Golgi apparatus By similarity
Note: Colocalized with RIPOR1 in the Golgi of serum-starved cells and relocated to cytoplasmic punctae, probably vesicular compartments, along with RIPOR1 upon serum stimulation in a Rho- and PDCD10-dependent manner.By similarity
Cytosol
- cytosol Source: MGI
Golgi apparatus
- Golgi apparatus Source: UniProtKB
- Golgi membrane Source: MGI
Plasma Membrane
- apical plasma membrane Source: MGI
Other locations
- cell periphery Source: MGI
- cytoplasm Source: UniProtKB
- Golgi-associated vesicle Source: MGI
- membrane Source: UniProtKB
- perinuclear region of cytoplasm Source: MGI
Keywords - Cellular componenti
Cytoplasm, Golgi apparatusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000086405 | 2 – 416 | Serine/threonine-protein kinase 26Add BLAST | 415 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineBy similarity | 1 | |
Modified residuei | 4 | PhosphoserineBy similarity | 1 | |
Modified residuei | 178 | Phosphothreonine; by autocatalysisBy similarity | 1 | |
Modified residuei | 300 | PhosphoserineCombined sources | 1 | |
Modified residuei | 304 | PhosphoserineBy similarity | 1 | |
Modified residuei | 306 | PhosphoserineBy similarity | 1 | |
Modified residuei | 309 | PhosphoserineCombined sources | 1 | |
Modified residuei | 325 | PhosphoserineBy similarity | 1 | |
Modified residuei | 327 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 328 | PhosphothreonineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q99JT2 |
jPOSTi | Q99JT2 |
MaxQBi | Q99JT2 |
PaxDbi | Q99JT2 |
PRIDEi | Q99JT2 |
ProteomicsDBi | 254761 |
PTM databases
iPTMneti | Q99JT2 |
PhosphoSitePlusi | Q99JT2 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000031112, Expressed in humerus cartilage element and 274 other tissues |
ExpressionAtlasi | Q99JT2, baseline and differential |
Genevisiblei | Q99JT2, MM |
Interactioni
Subunit structurei
Homodimer.
Interacts with PDCD10.
Interacts with GOLGA2.
Interacts with CTTNBP2NL.
Interacts with RIPOR1 (via C-terminus); this interaction occurs in a PDCD10-dependent and Rho-independent manner.
Interacts with PDCD10; this interaction is required for the association of STK26 with RIPOR1.
By similarityGO - Molecular functioni
- identical protein binding Source: MGI
- protein homodimerization activity Source: MGI
Protein-protein interaction databases
BioGRIDi | 214035, 6 interactors |
IntActi | Q99JT2, 17 interactors |
STRINGi | 10090.ENSMUSP00000033444 |
Miscellaneous databases
RNActi | Q99JT2, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 24 – 274 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 251 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 296 – 343 | DisorderedSequence analysisAdd BLAST | 48 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 327 – 341 | Basic and acidic residuesSequence analysisAdd BLAST | 15 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0201, Eukaryota |
GeneTreei | ENSGT00940000157904 |
InParanoidi | Q99JT2 |
OMAi | ECKLLAQ |
OrthoDBi | 967913at2759 |
PhylomeDBi | Q99JT2 |
TreeFami | TF354217 |
Family and domain databases
CDDi | cd06640, STKc_MST4, 1 hit |
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR035056, STK_MST4 |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MAHSPVAVQV PGMQNNIADP EELFTKLERI GKGSFGEVFK GIDNRTQQVV
60 70 80 90 100
AIKIIDLEEA EDEIEDIQQE ITVLSQCDSS YVTKYYGSYL KGSKLWIIME
110 120 130 140 150
YLGGGSALDL LRAGPFDEFQ IATMLKEILK GLDYLHSEKK IHRDIKAANV
160 170 180 190 200
LLSEQGDVKL ADFGVAGQLT DTQIKRNTFV GTPFWMAPEV IQQSAYDSKA
210 220 230 240 250
DIWSLGITAI ELAKGEPPNS DMHPMRVLFL IPKNNPPTLI GDFTKSFKEF
260 270 280 290 300
IDACLNKDPS FRPTAKELLK HKFIVKNSKK TSYLTELIDR FKRWKAEGHS
310 320 330 340 350
DEESDSEGSD SESSSRESNP HPEWSFTTVR KKPDPKKLQN GEEQDLVQTL
360 370 380 390 400
SCLSMIITPA FAELKQQDEN NASRNQAIEE LEKSIAVAET ACPGITDKMV
410
KKLIEKFQKC SADESP
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA2AD84 | A2AD84_MOUSE | Serine/threonine-protein kinase 26 | Stk26 2610018G03Rik | 392 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK034609 mRNA Translation: BAC28768.1 BC005708 mRNA Translation: AAH05708.1 |
CCDSi | CCDS40968.1 |
RefSeqi | NP_001300673.1, NM_001313744.1 NP_598490.1, NM_133729.2 |
Genome annotation databases
Ensembli | ENSMUST00000033444; ENSMUSP00000033444; ENSMUSG00000031112 |
GeneIDi | 70415 |
KEGGi | mmu:70415 |
UCSCi | uc009tdr.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK034609 mRNA Translation: BAC28768.1 BC005708 mRNA Translation: AAH05708.1 |
CCDSi | CCDS40968.1 |
RefSeqi | NP_001300673.1, NM_001313744.1 NP_598490.1, NM_133729.2 |
3D structure databases
AlphaFoldDBi | Q99JT2 |
SMRi | Q99JT2 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 214035, 6 interactors |
IntActi | Q99JT2, 17 interactors |
STRINGi | 10090.ENSMUSP00000033444 |
PTM databases
iPTMneti | Q99JT2 |
PhosphoSitePlusi | Q99JT2 |
Proteomic databases
EPDi | Q99JT2 |
jPOSTi | Q99JT2 |
MaxQBi | Q99JT2 |
PaxDbi | Q99JT2 |
PRIDEi | Q99JT2 |
ProteomicsDBi | 254761 |
Protocols and materials databases
Antibodypediai | 30220, 289 antibodies from 37 providers |
DNASUi | 70415 |
Genome annotation databases
Ensembli | ENSMUST00000033444; ENSMUSP00000033444; ENSMUSG00000031112 |
GeneIDi | 70415 |
KEGGi | mmu:70415 |
UCSCi | uc009tdr.1, mouse |
Organism-specific databases
CTDi | 51765 |
MGIi | MGI:1917665, Stk26 |
VEuPathDBi | HostDB:ENSMUSG00000031112 |
Phylogenomic databases
eggNOGi | KOG0201, Eukaryota |
GeneTreei | ENSGT00940000157904 |
InParanoidi | Q99JT2 |
OMAi | ECKLLAQ |
OrthoDBi | 967913at2759 |
PhylomeDBi | Q99JT2 |
TreeFami | TF354217 |
Enzyme and pathway databases
Reactomei | R-MMU-111465, Apoptotic cleavage of cellular proteins |
Miscellaneous databases
BioGRID-ORCSi | 70415, 2 hits in 74 CRISPR screens |
ChiTaRSi | Stk26, mouse |
PROi | PR:Q99JT2 |
RNActi | Q99JT2, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000031112, Expressed in humerus cartilage element and 274 other tissues |
ExpressionAtlasi | Q99JT2, baseline and differential |
Genevisiblei | Q99JT2, MM |
Family and domain databases
CDDi | cd06640, STKc_MST4, 1 hit |
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR035056, STK_MST4 |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | STK26_MOUSE | |
Accessioni | Q99JT2Primary (citable) accession number: Q99JT2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 16, 2005 |
Last sequence update: | June 1, 2001 | |
Last modified: | May 25, 2022 | |
This is version 161 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human and mouse protein kinases
Human and mouse protein kinases: classification and index - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families