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Protein

Mitogen-activated protein kinase kinase kinase kinase 3

Gene

Map4k3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.By similarity

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei45ATPPROSITE-ProRule annotation1
Active sitei136Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi22 – 30ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase kinase kinase kinase 3 (EC:2.7.11.1)
Alternative name(s):
Germinal center kinase-related protein kinase
Short name:
GLK
MAPK/ERK kinase kinase kinase 3
Short name:
MEK kinase kinase 3
Short name:
MEKKK 3
Gene namesi
Name:Map4k3
OrganismiMus musculus (Mouse)Imported
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:2154405 Map4k3

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000862781 – 894Mitogen-activated protein kinase kinase kinase kinase 3Add BLAST894

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei329PhosphoserineCombined sources1
Modified residuei398PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ99JP0
PaxDbiQ99JP0
PeptideAtlasiQ99JP0
PRIDEiQ99JP0

PTM databases

iPTMnetiQ99JP0
PhosphoSitePlusiQ99JP0

Expressioni

Gene expression databases

BgeeiENSMUSG00000024242 Expressed in 312 organ(s), highest expression level in vestibular membrane of cochlear duct
ExpressionAtlasiQ99JP0 baseline and differential
GenevisibleiQ99JP0 MM

Interactioni

Subunit structurei

Interacts with SH3GL2. Interaction appears to regulate MAP4K3-mediated JNK activation (By similarity).By similarity

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ99JP0, 3 interactors
STRINGi10090.ENSMUSP00000108008

Structurei

3D structure databases

ProteinModelPortaliQ99JP0
SMRiQ99JP0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini16 – 273Protein kinasePROSITE-ProRule annotationAdd BLAST258
Domaini556 – 867CNHPROSITE-ProRule annotationAdd BLAST312

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0576 Eukaryota
ENOG410XP9G LUCA
GeneTreeiENSGT00920000148966
HOGENOMiHOG000230824
HOVERGENiHBG036702
InParanoidiQ99JP0
KOiK04406

Family and domain databases

InterProiView protein in InterPro
IPR001180 CNH_dom
IPR011009 Kinase-like_dom_sf
IPR021160 MAPKKKK
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
PfamiView protein in Pfam
PF00780 CNH, 1 hit
PF00069 Pkinase, 1 hit
PIRSFiPIRSF038172 MAPKKKK, 1 hit
SMARTiView protein in SMART
SM00036 CNH, 1 hit
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS50219 CNH, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q99JP0-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNPGFDLSRR NPQEDFELIQ RIGSGTYGDV YKARNVNTGE LAAIKVIKLE
60 70 80 90 100
PGEDFAVVQQ EIIMMKDCKH PNIVAYFGSY LRRDKLWICM EFCGGGSLQD
110 120 130 140 150
IYHVTGPLSE LQIAYVSRET LQGLYYLHSK GKMHRDIKGA NILLTDNGHV
160 170 180 190 200
KLADFGVSAQ ITATIAKRKS FIGTPYWMAP EVAAVERKGG YNQLCDLWAV
210 220 230 240 250
GITAIELAEL QPPMFDLHPM RALFLMTKSN FQPPKLKDKL KWSNSFHHFV
260 270 280 290 300
KMALTKNPKK RPNAEKLLQH PFVTQPLTRS LAIELLDKVN NPDHSTYHDF
310 320 330 340 350
DDDDPEPLVA VPHRIPSTSR NVREEKTRSE INFGQVKFDP PLRKETEPHH
360 370 380 390 400
ELPDSDGFFD SSEEIYYTAR SNLDLQLEYG QGHQSHCFLG GNKSLLKSVE
410 420 430 440 450
EELHQRGHVA HLEDDEGDDD DSKHSTMKAK VPPPLPPKPK SIFIPQDTHS
460 470 480 490 500
AEDGNQGTIK RCPSSGSPAK PSHVPPRPPP PRLPPQKPAV LGNGVNSFQL
510 520 530 540 550
NGERDGSLYQ QQSEQRGTNL SRKEKKDVPK PISNGLPPTP KVHMGACFSK
560 570 580 590 600
VFNGCPLKIH CATSWINPDT RDQYLIFGAE EGIYTLNLNE LHETSMEQLF
610 620 630 640 650
PRRCTWLYVM NNCLLSVSGK ASQLYSHNLP GLFDYARQMQ KLPVAIPAHK
660 670 680 690 700
LPDRILPRKF AVSAKIPETK WCQKCCVVRN PYTGHKYLCG ALQTSIVLLE
710 720 730 740 750
WVEPMQKFML IKHIEFPMPC PLRMFEMLVV PEQEYPLVCV GVSRGRDFNQ
760 770 780 790 800
VVRFETVNPN STSSWFTESD APQTSVTHVT QLERDTILVC LDCCIKIVNL
810 820 830 840 850
QGRLKSSRKL SSELTFDFQI ESIVCLQDSV LAFWKHGMQG RSFRSNEVTQ
860 870 880 890
EISDNTRIFR LLGSDRVVVL ESRPTDNPTA NSNLYILAGH ENSY
Length:894
Mass (Da):101,119
Last modified:July 27, 2011 - v4
Checksum:iA9D9086ADC9DDA4F
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9QNE9E9QNE9_MOUSE
Mitogen-activated protein kinase ki...
Map4k3
896Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti874P → R in AAH05781 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC131712 Genomic DNA No translation available.
AC169501 Genomic DNA No translation available.
AK014711 mRNA Translation: BAB29516.1
BC005781 mRNA Translation: AAH05781.1
CCDSiCCDS70847.1
RefSeqiNP_001277274.1, NM_001290345.1
UniGeneiMm.311948

Genome annotation databases

EnsembliENSMUST00000025089; ENSMUSP00000025089; ENSMUSG00000024242
GeneIDi225028
KEGGimmu:225028
UCSCiuc008drj.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC131712 Genomic DNA No translation available.
AC169501 Genomic DNA No translation available.
AK014711 mRNA Translation: BAB29516.1
BC005781 mRNA Translation: AAH05781.1
CCDSiCCDS70847.1
RefSeqiNP_001277274.1, NM_001290345.1
UniGeneiMm.311948

3D structure databases

ProteinModelPortaliQ99JP0
SMRiQ99JP0
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ99JP0, 3 interactors
STRINGi10090.ENSMUSP00000108008

PTM databases

iPTMnetiQ99JP0
PhosphoSitePlusiQ99JP0

Proteomic databases

MaxQBiQ99JP0
PaxDbiQ99JP0
PeptideAtlasiQ99JP0
PRIDEiQ99JP0

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025089; ENSMUSP00000025089; ENSMUSG00000024242
GeneIDi225028
KEGGimmu:225028
UCSCiuc008drj.2 mouse

Organism-specific databases

CTDi8491
MGIiMGI:2154405 Map4k3

Phylogenomic databases

eggNOGiKOG0576 Eukaryota
ENOG410XP9G LUCA
GeneTreeiENSGT00920000148966
HOGENOMiHOG000230824
HOVERGENiHBG036702
InParanoidiQ99JP0
KOiK04406

Miscellaneous databases

PROiPR:Q99JP0
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024242 Expressed in 312 organ(s), highest expression level in vestibular membrane of cochlear duct
ExpressionAtlasiQ99JP0 baseline and differential
GenevisibleiQ99JP0 MM

Family and domain databases

InterProiView protein in InterPro
IPR001180 CNH_dom
IPR011009 Kinase-like_dom_sf
IPR021160 MAPKKKK
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
PfamiView protein in Pfam
PF00780 CNH, 1 hit
PF00069 Pkinase, 1 hit
PIRSFiPIRSF038172 MAPKKKK, 1 hit
SMARTiView protein in SMART
SM00036 CNH, 1 hit
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS50219 CNH, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiM4K3_MOUSE
AccessioniPrimary (citable) accession number: Q99JP0
Secondary accession number(s): E9PWI4, Q9CUS8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 28, 2003
Last sequence update: July 27, 2011
Last modified: September 12, 2018
This is version 142 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  2. SIMILARITY comments
    Index of protein domains and families
  3. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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