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Protein

Sperm flagellar protein 1

Gene

Spef1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Microtubule-associated protein that promotes microtubule bundling and stabilizes microtubules against depolymerization in response to cold shock (PubMed:16206169). Microtubule-associated protein involved in the stabilization of microtubules along the axis of migration during radial intercalation. Promotes the establishment and stabilization of an axis of microtubules required for the active migration of cells into the outer epithelium (By similarity).By similarity1 Publication

Miscellaneous

Radial intercalation is a developmentally reiterated form of migration by which cells move in a direction orthogonal to the plane of the tissue from an inner layer to an outer layer.By similarity

GO - Molecular functioni

  • microtubule binding Source: UniProtKB

GO - Biological processi

  • cell migration Source: UniProtKB
  • cilium-dependent cell motility Source: GO_Central
  • negative regulation of microtubule depolymerization Source: UniProtKB

Names & Taxonomyi

Protein namesi
Recommended name:
Sperm flagellar protein 1
Alternative name(s):
Calponin-homology and microtubule-associated protein1 Publication
Gene namesi
Name:Spef1
Synonyms:Clamp1 Publication
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:3513546 Spef1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell projection, Cilium, Cytoplasm, Cytoskeleton, Flagellum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000794221 – 234Sperm flagellar protein 1Add BLAST234

Proteomic databases

MaxQBiQ99JL1
PaxDbiQ99JL1
PRIDEiQ99JL1

PTM databases

iPTMnetiQ99JL1
PhosphoSitePlusiQ99JL1

Expressioni

Tissue specificityi

Expressed predominantly in the seminiferous epithelium of adult testis (PubMed:15979255). Expressed in pillar cells of the organ of Corti (at protein level). Expressed in brain, kidney, lung and testis (PubMed:16206169).2 Publications

Developmental stagei

Expression is first detected in the testis at 3 weeks and continues to adulthood.1 Publication

Gene expression databases

BgeeiENSMUSG00000027329 Expressed in 170 organ(s), highest expression level in olfactory epithelium
CleanExiMM_SPEF1
ExpressionAtlasiQ99JL1 baseline and differential
GenevisibleiQ99JL1 MM

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000028801

Structurei

3D structure databases

ProteinModelPortaliQ99JL1
SMRiQ99JL1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 112Calponin-homology (CH)PROSITE-ProRule annotationAdd BLAST106

Phylogenomic databases

eggNOGiENOG410IYU4 Eukaryota
ENOG4111WVQ LUCA
GeneTreeiENSGT00910000144159
HOGENOMiHOG000231709
HOVERGENiHBG051156
InParanoidiQ99JL1
OMAiREKDYTI
OrthoDBiEOG091G0V3B
PhylomeDBiQ99JL1
TreeFamiTF323506

Family and domain databases

Gene3Di1.10.418.10, 1 hit
InterProiView protein in InterPro
IPR001715 CH-domain
IPR010441 CH_2
IPR036872 CH_dom_sf
PfamiView protein in Pfam
PF06294 CH_2, 1 hit
SUPFAMiSSF47576 SSF47576, 1 hit
PROSITEiView protein in PROSITE
PS50021 CH, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q99JL1-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MESSVDEEAL HQLYLWVDNI PLSRPKRNLS RDFSDGVLVA ELIKFYFPKM
60 70 80 90 100
VEMHNYVPAN SLQQKLSNWG HLNRKVLNKL NFSVPDDVMR KIAQCSPGVV
110 120 130 140 150
ELVLIPLRQR LEERQRRQKL GVGSLQELAP QDSSGYMDMG LPQKVRGEGA
160 170 180 190 200
PALGEQLREG RPLASRPPGY NQALQGDPSF VLQIAEKEQE LLASQETVQV
210 220 230
LQMKVKRLEH LLQLKNVRID DLSRRLQQAE RKQR
Length:234
Mass (Da):26,839
Last modified:June 1, 2001 - v1
Checksum:i1772A71278845F44
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F6ZW13F6ZW13_MOUSE
Sperm flagellar protein 1
Spef1
171Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti111L → P in AK016476 (PubMed:16141072).Curated1
Sequence conflicti167P → L in AK016476 (PubMed:16141072).Curated1
Sequence conflicti187 – 233KEQEL…AERKQ → RSRSCWPLKRQYRSF in AK016476 (PubMed:16141072).CuratedAdd BLAST47

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY860964 mRNA Translation: AAX56299.1
AK016476 mRNA No translation available.
AK046964 mRNA Translation: BAC32928.1
AK047675 mRNA Translation: BAC33121.1
AL831736 Genomic DNA No translation available.
BC006046 mRNA Translation: AAH06046.1
CCDSiCCDS38245.1
RefSeqiNP_081917.1, NM_027641.2
UniGeneiMm.252722

Genome annotation databases

EnsembliENSMUST00000028801; ENSMUSP00000028801; ENSMUSG00000027329
ENSMUST00000110218; ENSMUSP00000105847; ENSMUSG00000027329
GeneIDi70997
KEGGimmu:70997
UCSCiuc008mkv.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY860964 mRNA Translation: AAX56299.1
AK016476 mRNA No translation available.
AK046964 mRNA Translation: BAC32928.1
AK047675 mRNA Translation: BAC33121.1
AL831736 Genomic DNA No translation available.
BC006046 mRNA Translation: AAH06046.1
CCDSiCCDS38245.1
RefSeqiNP_081917.1, NM_027641.2
UniGeneiMm.252722

3D structure databases

ProteinModelPortaliQ99JL1
SMRiQ99JL1
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000028801

PTM databases

iPTMnetiQ99JL1
PhosphoSitePlusiQ99JL1

Proteomic databases

MaxQBiQ99JL1
PaxDbiQ99JL1
PRIDEiQ99JL1

Protocols and materials databases

DNASUi70997
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028801; ENSMUSP00000028801; ENSMUSG00000027329
ENSMUST00000110218; ENSMUSP00000105847; ENSMUSG00000027329
GeneIDi70997
KEGGimmu:70997
UCSCiuc008mkv.1 mouse

Organism-specific databases

CTDi25876
MGIiMGI:3513546 Spef1

Phylogenomic databases

eggNOGiENOG410IYU4 Eukaryota
ENOG4111WVQ LUCA
GeneTreeiENSGT00910000144159
HOGENOMiHOG000231709
HOVERGENiHBG051156
InParanoidiQ99JL1
OMAiREKDYTI
OrthoDBiEOG091G0V3B
PhylomeDBiQ99JL1
TreeFamiTF323506

Miscellaneous databases

ChiTaRSiSpef1 mouse
PROiPR:Q99JL1
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027329 Expressed in 170 organ(s), highest expression level in olfactory epithelium
CleanExiMM_SPEF1
ExpressionAtlasiQ99JL1 baseline and differential
GenevisibleiQ99JL1 MM

Family and domain databases

Gene3Di1.10.418.10, 1 hit
InterProiView protein in InterPro
IPR001715 CH-domain
IPR010441 CH_2
IPR036872 CH_dom_sf
PfamiView protein in Pfam
PF06294 CH_2, 1 hit
SUPFAMiSSF47576 SSF47576, 1 hit
PROSITEiView protein in PROSITE
PS50021 CH, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSPEF1_MOUSE
AccessioniPrimary (citable) accession number: Q99JL1
Secondary accession number(s): A2ANS9, Q543S5, Q9D4J8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2002
Last sequence update: June 1, 2001
Last modified: November 7, 2018
This is version 112 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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