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Protein

Protein kinase C and casein kinase II substrate protein 3

Gene

Pacsin3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a role in endocytosis and regulates internalization of plasma membrane proteins. Overexpression impairs internalization of SLC2A1/GLUT1 and TRPV4 and increases the levels of SLC2A1/GLUT1 and TRPV4 at the cell membrane. Inhibits the TRPV4 calcium channel activity.4 Publications

GO - Molecular functioni

  • calcium channel inhibitor activity Source: UniProtKB
  • cytoskeletal protein binding Source: MGI
  • lipid binding Source: UniProtKB
  • phospholipid binding Source: GO_Central

GO - Biological processi

Keywordsi

Biological processEndocytosis
LigandLipid-binding

Enzyme and pathway databases

ReactomeiR-MMU-8856828 Clathrin-mediated endocytosis

Names & Taxonomyi

Protein namesi
Recommended name:
Protein kinase C and casein kinase II substrate protein 3
Gene namesi
Name:Pacsin3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1891410 Pacsin3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi415P → L: Loss of DNM1-, SYNJ1- and WASL-binding. Loss of effect on transferrin endocytosis. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001618011 – 424Protein kinase C and casein kinase II substrate protein 3Add BLAST424

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei276PhosphoserineBy similarity1
Modified residuei303PhosphoserineCombined sources1
Modified residuei319PhosphoserineCombined sources1
Modified residuei324PhosphothreonineBy similarity1
Modified residuei327PhosphoserineBy similarity1
Modified residuei354PhosphoserineCombined sources1
Modified residuei383PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated by casein kinase 2 (CK2) and protein kinase C (PKC).

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ99JB8
PaxDbiQ99JB8
PRIDEiQ99JB8

PTM databases

iPTMnetiQ99JB8
PhosphoSitePlusiQ99JB8
SwissPalmiQ99JB8

Expressioni

Tissue specificityi

Highly expressed in skeletal muscle, heart and lung; also detected in brain, kidney and uterus (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000027257 Expressed in 227 organ(s), highest expression level in skeletal muscle tissue
ExpressionAtlasiQ99JB8 baseline and differential
GenevisibleiQ99JB8 MM

Interactioni

Subunit structurei

Homodimer. May form heterooligomers with other PACSINs. Interacts (via SH3 domain) with DNM1, SYNJ1 and WASL. Interacts with TRPV4.4 Publications

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ99JB8, 4 interactors
MINTiQ99JB8
STRINGi10090.ENSMUSP00000028694

Structurei

Secondary structure

1424
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ99JB8
SMRiQ99JB8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini10 – 280F-BARPROSITE-ProRule annotationAdd BLAST271
Domaini363 – 424SH3PROSITE-ProRule annotationAdd BLAST62

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili23 – 2721 PublicationAdd BLAST250

Domaini

The F-BAR domain forms a coiled coil and mediates membrane-binding and membrane tubulation.1 Publication

Sequence similaritiesi

Belongs to the PACSIN family.Curated

Keywords - Domaini

Coiled coil, SH3 domain

Phylogenomic databases

eggNOGiKOG2856 Eukaryota
ENOG410XRX2 LUCA
GeneTreeiENSGT00510000046376
HOGENOMiHOG000007245
HOVERGENiHBG053486
InParanoidiQ99JB8
KOiK20123
OMAiESCQAAE
OrthoDBiEOG091G0AS9
PhylomeDBiQ99JB8
TreeFamiTF313677

Family and domain databases

Gene3Di1.20.1270.60, 1 hit
InterProiView protein in InterPro
IPR027267 AH/BAR_dom_sf
IPR031160 F_BAR
IPR001060 FCH_dom
IPR028523 PACSIN3
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
PANTHERiPTHR23065:SF18 PTHR23065:SF18, 1 hit
PfamiView protein in Pfam
PF00611 FCH, 1 hit
PF14604 SH3_9, 1 hit
PRINTSiPR00452 SH3DOMAIN
SMARTiView protein in SMART
SM00055 FCH, 1 hit
SM00326 SH3, 1 hit
SUPFAMiSSF103657 SSF103657, 1 hit
SSF50044 SSF50044, 1 hit
PROSITEiView protein in PROSITE
PS51741 F_BAR, 1 hit
PS50002 SH3, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 7 potential isoforms that are computationally mapped.Show allAlign All

Q99JB8-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAPEEDAGGE VLGGSFWEAG NYRRTVQRVE DGHRLCGDLV SCFQERARIE
60 70 80 90 100
KAYAQQLADW ARKWRGAVEK GPQYGTLEKA WHAFFTAAER LSELHLEVRE
110 120 130 140 150
KLHGPDSERV RTWQRGAFHR PVLGGFRESR AAEDGFRKAQ KPWLKRLKEV
160 170 180 190 200
EASKKSYHTA RKDEKTAQTR ESHAKADSSM SQEQLRKLQE RVGRCTKEAE
210 220 230 240 250
KMKTQYEQTL AELNRYTPRY MEDMEQAFES CQAAERQRLL FFKDVLLTLH
260 270 280 290 300
QHLDLSSSDK FHELHRDLQQ SIEAASDEED LRWWRSTHGP GMAMNWPQFE
310 320 330 340 350
EWSLDTQRAI SRKEKGGRSP DEVTLTSIVP TRDGTAPPPQ SPSSPGSGQD
360 370 380 390 400
EDWSDEESPR KVATGVRVRA LYDYAGQEAD ELSFRAGEEL LKMSEEDEQG
410 420
WCQGQLQSGR IGLYPANYVE CVGA
Length:424
Mass (Da):48,585
Last modified:June 1, 2001 - v1
Checksum:i00475BC0321485B7
GO

Computationally mapped potential isoform sequencesi

There are 7 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A6PWR1A6PWR1_MOUSE
Protein kinase C and casein kinase ...
Pacsin3
178Annotation score:
B1AW91B1AW91_MOUSE
Protein kinase C and casein kinase ...
Pacsin3
168Annotation score:
B1AW92B1AW92_MOUSE
Protein kinase C and casein kinase ...
Pacsin3
95Annotation score:
B1AW93B1AW93_MOUSE
Protein kinase C and casein kinase ...
Pacsin3
125Annotation score:
B1AW95B1AW95_MOUSE
Protein kinase C and casein kinase ...
Pacsin3
64Annotation score:
B1AW94B1AW94_MOUSE
Protein kinase C and casein kinase ...
Pacsin3
114Annotation score:
F6W8Y8F6W8Y8_MOUSE
Protein kinase C and casein kinase ...
Pacsin3
133Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti360R → G in AAG31022 (PubMed:11082044).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF149824 mRNA Translation: AAG31022.1
AF242531 mRNA Translation: AAK29208.1
BC003884 mRNA Translation: AAH03884.1
CCDSiCCDS16429.1
RefSeqiNP_001276606.1, NM_001289677.1
NP_001276607.1, NM_001289678.1
NP_083009.1, NM_028733.4
NP_112019.2, NM_030880.3
XP_006500487.1, XM_006500424.3
XP_006500488.1, XM_006500425.1
XP_006500490.1, XM_006500427.3
XP_006500491.1, XM_006500428.3
UniGeneiMm.236650

Genome annotation databases

EnsembliENSMUST00000028694; ENSMUSP00000028694; ENSMUSG00000027257
ENSMUST00000059566; ENSMUSP00000054391; ENSMUSG00000027257
ENSMUST00000111349; ENSMUSP00000106981; ENSMUSG00000027257
ENSMUST00000168916; ENSMUSP00000129175; ENSMUSG00000027257
GeneIDi80708
KEGGimmu:80708
UCSCiuc008kvm.2 mouse

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF149824 mRNA Translation: AAG31022.1
AF242531 mRNA Translation: AAK29208.1
BC003884 mRNA Translation: AAH03884.1
CCDSiCCDS16429.1
RefSeqiNP_001276606.1, NM_001289677.1
NP_001276607.1, NM_001289678.1
NP_083009.1, NM_028733.4
NP_112019.2, NM_030880.3
XP_006500487.1, XM_006500424.3
XP_006500488.1, XM_006500425.1
XP_006500490.1, XM_006500427.3
XP_006500491.1, XM_006500428.3
UniGeneiMm.236650

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3M3WX-ray2.60A/B1-320[»]
3QE6X-ray2.60A/B1-304[»]
3SYVX-ray3.10A/B/C/D/E/F/G/H1-341[»]
ProteinModelPortaliQ99JB8
SMRiQ99JB8
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ99JB8, 4 interactors
MINTiQ99JB8
STRINGi10090.ENSMUSP00000028694

PTM databases

iPTMnetiQ99JB8
PhosphoSitePlusiQ99JB8
SwissPalmiQ99JB8

Proteomic databases

MaxQBiQ99JB8
PaxDbiQ99JB8
PRIDEiQ99JB8

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028694; ENSMUSP00000028694; ENSMUSG00000027257
ENSMUST00000059566; ENSMUSP00000054391; ENSMUSG00000027257
ENSMUST00000111349; ENSMUSP00000106981; ENSMUSG00000027257
ENSMUST00000168916; ENSMUSP00000129175; ENSMUSG00000027257
GeneIDi80708
KEGGimmu:80708
UCSCiuc008kvm.2 mouse

Organism-specific databases

CTDi29763
MGIiMGI:1891410 Pacsin3

Phylogenomic databases

eggNOGiKOG2856 Eukaryota
ENOG410XRX2 LUCA
GeneTreeiENSGT00510000046376
HOGENOMiHOG000007245
HOVERGENiHBG053486
InParanoidiQ99JB8
KOiK20123
OMAiESCQAAE
OrthoDBiEOG091G0AS9
PhylomeDBiQ99JB8
TreeFamiTF313677

Enzyme and pathway databases

ReactomeiR-MMU-8856828 Clathrin-mediated endocytosis

Miscellaneous databases

PROiPR:Q99JB8
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027257 Expressed in 227 organ(s), highest expression level in skeletal muscle tissue
ExpressionAtlasiQ99JB8 baseline and differential
GenevisibleiQ99JB8 MM

Family and domain databases

Gene3Di1.20.1270.60, 1 hit
InterProiView protein in InterPro
IPR027267 AH/BAR_dom_sf
IPR031160 F_BAR
IPR001060 FCH_dom
IPR028523 PACSIN3
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
PANTHERiPTHR23065:SF18 PTHR23065:SF18, 1 hit
PfamiView protein in Pfam
PF00611 FCH, 1 hit
PF14604 SH3_9, 1 hit
PRINTSiPR00452 SH3DOMAIN
SMARTiView protein in SMART
SM00055 FCH, 1 hit
SM00326 SH3, 1 hit
SUPFAMiSSF103657 SSF103657, 1 hit
SSF50044 SSF50044, 1 hit
PROSITEiView protein in PROSITE
PS51741 F_BAR, 1 hit
PS50002 SH3, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPACN3_MOUSE
AccessioniPrimary (citable) accession number: Q99JB8
Secondary accession number(s): Q9EQP9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 2002
Last sequence update: June 1, 2001
Last modified: November 7, 2018
This is version 143 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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