UniProtKB - Q99JA4 (CLTR1_MOUSE)
Protein
Cysteinyl leukotriene receptor 1
Gene
Cysltr1
Organism
Mus musculus (Mouse)
Status
Functioni
Receptor for cysteinyl leukotrienes mediating constriction of the microvascular smooth muscle during an inflammatory response. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. The rank order of affinities for the leukotrienes is LTD4 >> LTE4 = LTC4 >> LTB4.
Miscellaneous
MK-571, a selective antagonist, was shown to inhibit eosinophilia, bronchial hyperreactivity and microvascular leakage. Zafirlukast (Accolate) and pranlukast (Onon) were also shown to be selective antagonists.
GO - Molecular functioni
- cysteinyl leukotriene receptor activity Source: GO_Central
- G protein-coupled peptide receptor activity Source: GO_Central
- leukotriene receptor activity Source: MGI
GO - Biological processi
- calcium ion transport Source: MGI
- cell surface receptor signaling pathway Source: MGI
- chemotaxis Source: MGI
- inflammatory response Source: MGI
- inflammatory response to antigenic stimulus Source: MGI
- neuropeptide signaling pathway Source: GO_Central
- positive regulation of angiogenesis Source: MGI
- positive regulation of vasoconstriction Source: MGI
Keywordsi
Molecular function | G-protein coupled receptor, Receptor, Transducer |
Enzyme and pathway databases
Reactomei | R-MMU-391906, Leukotriene receptors R-MMU-416476, G alpha (q) signalling events |
Names & Taxonomyi
Protein namesi | Recommended name: Cysteinyl leukotriene receptor 1Short name: CysLTR1 Alternative name(s): Cysteinyl leukotriene D4 receptor Short name: LTD4 receptor |
Gene namesi | Name:Cysltr1 Synonyms:Cyslt1, Cyslt1r |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1926218, Cysltr1 |
Subcellular locationi
Plasma membrane
Plasma Membrane
- integral component of plasma membrane Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 43 | ExtracellularSequence analysisAdd BLAST | 43 | |
Transmembranei | 44 – 64 | Helical; Name=1Sequence analysisAdd BLAST | 21 | |
Topological domaini | 65 – 72 | CytoplasmicSequence analysis | 8 | |
Transmembranei | 73 – 93 | Helical; Name=2Sequence analysisAdd BLAST | 21 | |
Topological domaini | 94 – 121 | ExtracellularSequence analysisAdd BLAST | 28 | |
Transmembranei | 122 – 142 | Helical; Name=3Sequence analysisAdd BLAST | 21 | |
Topological domaini | 143 – 156 | CytoplasmicSequence analysisAdd BLAST | 14 | |
Transmembranei | 157 – 177 | Helical; Name=4Sequence analysisAdd BLAST | 21 | |
Topological domaini | 178 – 208 | ExtracellularSequence analysisAdd BLAST | 31 | |
Transmembranei | 209 – 229 | Helical; Name=5Sequence analysisAdd BLAST | 21 | |
Topological domaini | 230 – 245 | CytoplasmicSequence analysisAdd BLAST | 16 | |
Transmembranei | 246 – 266 | Helical; Name=6Sequence analysisAdd BLAST | 21 | |
Topological domaini | 267 – 291 | ExtracellularSequence analysisAdd BLAST | 25 | |
Transmembranei | 292 – 312 | Helical; Name=7Sequence analysisAdd BLAST | 21 | |
Topological domaini | 313 – 352 | CytoplasmicSequence analysisAdd BLAST | 40 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000069300 | 1 – 352 | Cysteinyl leukotriene receptor 1Add BLAST | 352 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 15 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 19 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 26 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 111 ↔ 188 | PROSITE-ProRule annotation | ||
Glycosylationi | 184 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
jPOSTi | Q99JA4 |
PaxDbi | Q99JA4 |
PRIDEi | Q99JA4 |
PTM databases
GlyGeni | Q99JA4, 4 sites |
iPTMneti | Q99JA4 |
PhosphoSitePlusi | Q99JA4 |
Expressioni
Tissue specificityi
Widely expressed, with higher expression in the lung and skin, intermediate levels in the heart, kidney and stomach and lower levels in several other tissues. Isoform 1 is the most abundant form in all tested tissues.
Gene expression databases
Bgeei | ENSMUSG00000052821, Expressed in bone marrow macrophage and 128 other tissues |
Genevisiblei | Q99JA4, MM |
Interactioni
Binary interactionsi
Q99JA4
With | #Exp. | IntAct |
---|---|---|
Gpr17 [Q6NS65] | 2 | EBI-15791392,EBI-15791369 |
Protein-protein interaction databases
BioGRIDi | 208449, 1 interactor |
DIPi | DIP-48916N |
IntActi | Q99JA4, 1 interactor |
STRINGi | 10090.ENSMUSP00000109108 |
Chemistry databases
BindingDBi | Q99JA4 |
Miscellaneous databases
RNActi | Q99JA4, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502QUJU, Eukaryota |
GeneTreei | ENSGT01010000222255 |
HOGENOMi | CLU_009579_8_2_1 |
InParanoidi | Q99JA4 |
OMAi | PQDNQAK |
OrthoDBi | 791328at2759 |
PhylomeDBi | Q99JA4 |
TreeFami | TF350009 |
Family and domain databases
InterProi | View protein in InterPro IPR013310, CLT1_recept IPR004071, Cyst_leuk_rcpt IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR01902, CYSLT1RECPTR PR01533, CYSLTRECPTR PR00237, GPCRRHODOPSN |
PROSITEi | View protein in PROSITE PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: Q99JA4-1) [UniParc]FASTAAdd to basket
Also known as: Long
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MYLQGTKQTF LENMNGTENL TTSLINNTCH DTIDEFRNQV YSTMYSVISV
60 70 80 90 100
VGFFGNSFVL YVLIKTYHEK SAFQVYMINL AIADLLCVCT LPLRVVYYVH
110 120 130 140 150
KGKWLFGDFL CRLTTYALYV NLYCSIFFMT AMSFFRCVAI VFPVQNINLV
160 170 180 190 200
TQKKARFVCI GIWIFVILTS SPFLMYKSYQ DEKNNTKCFE PPQNNQAKKY
210 220 230 240 250
VLILHYVSLF FGFIIPFVTI IVCYTMIILT LLKNTMKKNM PSRRKAIGMI
260 270 280 290 300
IVVTAAFLVS FMPYHIQRTI HLHLLHSETR PCDSVLRMQK SVVITLSLAA
310 320 330 340 350
SNCCFDPLLY FFSGGNFRRR LSTFRKHSLS SMTYVPKKKA SLPEKGEEIC
NE
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 176 | Y → D in AAF73047 (PubMed:11342226).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_001921 | 1 – 13 | Missing in isoform 2. 1 PublicationAdd BLAST | 13 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF329272 Genomic DNA Translation: AAK16715.1 AF329272 Genomic DNA Translation: AAK16716.1 AF205830 mRNA Translation: AAK15433.1 AF263370 mRNA Translation: AAF73047.1 AB044087 mRNA Translation: BAA96809.1 AK033476 mRNA Translation: BAC28308.1 AL732460 Genomic DNA No translation available. BC027102 mRNA Translation: AAH27102.1 |
CCDSi | CCDS41098.1 [Q99JA4-1] |
RefSeqi | NP_001268788.1, NM_001281859.1 [Q99JA4-1] NP_001268791.1, NM_001281862.1 [Q99JA4-2] NP_067451.2, NM_021476.5 [Q99JA4-1] |
Genome annotation databases
Ensembli | ENSMUST00000064892; ENSMUSP00000063520; ENSMUSG00000052821 [Q99JA4-1] ENSMUST00000113480; ENSMUSP00000109108; ENSMUSG00000052821 [Q99JA4-1] |
GeneIDi | 58861 |
KEGGi | mmu:58861 |
UCSCi | uc009ubt.2, mouse [Q99JA4-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF329272 Genomic DNA Translation: AAK16715.1 AF329272 Genomic DNA Translation: AAK16716.1 AF205830 mRNA Translation: AAK15433.1 AF263370 mRNA Translation: AAF73047.1 AB044087 mRNA Translation: BAA96809.1 AK033476 mRNA Translation: BAC28308.1 AL732460 Genomic DNA No translation available. BC027102 mRNA Translation: AAH27102.1 |
CCDSi | CCDS41098.1 [Q99JA4-1] |
RefSeqi | NP_001268788.1, NM_001281859.1 [Q99JA4-1] NP_001268791.1, NM_001281862.1 [Q99JA4-2] NP_067451.2, NM_021476.5 [Q99JA4-1] |
3D structure databases
SMRi | Q99JA4 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 208449, 1 interactor |
DIPi | DIP-48916N |
IntActi | Q99JA4, 1 interactor |
STRINGi | 10090.ENSMUSP00000109108 |
Chemistry databases
BindingDBi | Q99JA4 |
ChEMBLi | CHEMBL3808269 |
Protein family/group databases
GPCRDBi | Search... |
PTM databases
GlyGeni | Q99JA4, 4 sites |
iPTMneti | Q99JA4 |
PhosphoSitePlusi | Q99JA4 |
Proteomic databases
jPOSTi | Q99JA4 |
PaxDbi | Q99JA4 |
PRIDEi | Q99JA4 |
Protocols and materials databases
Antibodypediai | 548, 308 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000064892; ENSMUSP00000063520; ENSMUSG00000052821 [Q99JA4-1] ENSMUST00000113480; ENSMUSP00000109108; ENSMUSG00000052821 [Q99JA4-1] |
GeneIDi | 58861 |
KEGGi | mmu:58861 |
UCSCi | uc009ubt.2, mouse [Q99JA4-1] |
Organism-specific databases
CTDi | 10800 |
MGIi | MGI:1926218, Cysltr1 |
Phylogenomic databases
eggNOGi | ENOG502QUJU, Eukaryota |
GeneTreei | ENSGT01010000222255 |
HOGENOMi | CLU_009579_8_2_1 |
InParanoidi | Q99JA4 |
OMAi | PQDNQAK |
OrthoDBi | 791328at2759 |
PhylomeDBi | Q99JA4 |
TreeFami | TF350009 |
Enzyme and pathway databases
Reactomei | R-MMU-391906, Leukotriene receptors R-MMU-416476, G alpha (q) signalling events |
Miscellaneous databases
BioGRID-ORCSi | 58861, 1 hit in 17 CRISPR screens |
PROi | PR:Q99JA4 |
RNActi | Q99JA4, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000052821, Expressed in bone marrow macrophage and 128 other tissues |
Genevisiblei | Q99JA4, MM |
Family and domain databases
InterProi | View protein in InterPro IPR013310, CLT1_recept IPR004071, Cyst_leuk_rcpt IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR01902, CYSLT1RECPTR PR01533, CYSLTRECPTR PR00237, GPCRRHODOPSN |
PROSITEi | View protein in PROSITE PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CLTR1_MOUSE | |
Accessioni | Q99JA4Primary (citable) accession number: Q99JA4 Secondary accession number(s): Q544P1, Q9JJ71, Q9JK47 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 11, 2002 |
Last sequence update: | June 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 150 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- 7-transmembrane G-linked receptors
List of 7-transmembrane G-linked receptor entries - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families