UniProtKB - Q99J95 (CDK9_MOUSE)
Cyclin-dependent kinase 9
Cdk9
Functioni
Catalytic activityi
- EC:2.7.11.22By similarityThis reaction proceeds in the forwardBy similarity direction.
- EC:2.7.11.22By similarityThis reaction proceeds in the forwardBy similarity direction.
- EC:2.7.11.23By similarityThis reaction proceeds in the forwardBy similarity direction.
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 48 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 149 | Proton acceptorPROSITE-ProRule annotation | 1 | |
Binding sitei | 167 | ATPPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 25 – 33 | ATPPROSITE-ProRule annotation | 9 | |
Nucleotide bindingi | 104 – 106 | ATPPROSITE-ProRule annotation | 3 |
GO - Molecular functioni
- 7SK snRNA binding Source: UniProtKB
- ATP binding Source: UniProtKB-KW
- chromatin binding Source: UniProtKB
- cyclin-dependent protein serine/threonine kinase activity Source: MGI
- DNA binding Source: MGI
- protein kinase binding Source: MGI
- protein serine/threonine kinase activity Source: MGI
- protein serine kinase activity Source: RHEA
- protein threonine kinase activity Source: RHEA
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: MGI
- RNA polymerase II CTD heptapeptide repeat kinase activity Source: MGI
- snRNA binding Source: MGI
- transcription coactivator binding Source: MGI
- transcription factor binding Source: MGI
- transcription regulatory region sequence-specific DNA binding Source: BHF-UCL
GO - Biological processi
- cellular response to cytokine stimulus Source: MGI
- DNA repair Source: UniProtKB-KW
- negative regulation of cell cycle arrest Source: MGI
- negative regulation of mRNA polyadenylation Source: MGI
- phosphorylation of RNA polymerase II C-terminal domain Source: UniProtKB
- positive regulation of cardiac muscle hypertrophy Source: MGI
- positive regulation of histone H2B ubiquitination Source: UniProtKB
- positive regulation of histone phosphorylation Source: UniProtKB
- positive regulation of mRNA 3'-UTR binding Source: MGI
- positive regulation of transcription by RNA polymerase II Source: UniProtKB
- protein phosphorylation Source: MGI
- regulation of DNA repair Source: MGI
- regulation of histone modification Source: MGI
- regulation of muscle cell differentiation Source: MGI
- replication fork processing Source: MGI
- response to drug Source: Ensembl
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Biological process | DNA damage, DNA repair, Transcription, Transcription regulation |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-112382, Formation of RNA Pol II elongation complex R-MMU-2173796, SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription R-MMU-674695, RNA Polymerase II Pre-transcription Events R-MMU-6796648, TP53 Regulates Transcription of DNA Repair Genes R-MMU-6807505, RNA polymerase II transcribes snRNA genes R-MMU-75955, RNA Polymerase II Transcription Elongation R-MMU-9018519, Estrogen-dependent gene expression |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Cdk9 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1328368, Cdk9 |
Subcellular locationi
Nucleus
Cytoplasm and Cytosol
- Cytoplasm By similarity
Note: Accumulates on chromatin in response to replication stress. Complexed with CCNT1 in nuclear speckles, but uncomplexed form in the cytoplasm. The translocation from nucleus to cytoplasm is XPO1/CRM1-dependent. Associates with PML body when acetylated (By similarity).By similarity
Nucleus
- cyclin/CDK positive transcription elongation factor complex Source: MGI
- mediator complex Source: GO_Central
- nucleoplasm Source: MGI
- nucleus Source: MGI
- P-TEFb complex Source: MGI
- PML body Source: MGI
- transcription elongation factor complex Source: UniProtKB
Other locations
- cytoplasmic ribonucleoprotein granule Source: MGI
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000085801 | 1 – 372 | Cyclin-dependent kinase 9Add BLAST | 372 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 44 | N6-acetyllysine; by P300/CBP, PCAF/KAT2B and GCN5/KAT2ABy similarity | 1 | |
Modified residuei | 48 | N6-acetyllysine; by PCAF/KAT2B and GCN5/KAT2ABy similarity | 1 | |
Modified residuei | 175 | PhosphoserineBy similarity | 1 | |
Modified residuei | 186 | Phosphothreonine; by CaMK1DBy similarity | 1 | |
Modified residuei | 347 | Phosphoserine; by CDK9 and PKABy similarity | 1 | |
Modified residuei | 350 | Phosphothreonine; by CDK9By similarity | 1 | |
Modified residuei | 353 | Phosphoserine; by CDK9By similarity | 1 | |
Modified residuei | 354 | Phosphothreonine; by CDK9By similarity | 1 | |
Modified residuei | 357 | Phosphoserine; by CDK9By similarity | 1 | |
Modified residuei | 362 | Phosphothreonine; by CDK9By similarity | 1 | |
Modified residuei | 363 | Phosphothreonine; by CDK9By similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q99J95 |
jPOSTi | Q99J95 |
PaxDbi | Q99J95 |
PeptideAtlasi | Q99J95 |
PRIDEi | Q99J95 |
ProteomicsDBi | 280041 [Q99J95-1] 280042 [Q99J95-2] 280043 [Q99J95-3] |
PTM databases
iPTMneti | Q99J95 |
PhosphoSitePlusi | Q99J95 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000009555, Expressed in granulocyte and 314 other tissues |
ExpressionAtlasi | Q99J95, baseline and differential |
Genevisiblei | Q99J95, MM |
Interactioni
Subunit structurei
Component of the super elongation complex (SEC), at least composed of EAF1, EAF2, CDK9, MLLT3/AF9, AFF (AFF1 or AFF4), the P-TEFb complex and ELL (ELL, ELL2 or ELL3). Associates with CCNT1/cyclin-T1, CCNT2/cyclin-T2 (isoform A and isoform B) or CCNK/cyclin-K to form active P-TEFb. P-TEFb forms a complex with AFF4/AF5Q31 and is part of the super elongation complex (SEC).
Component of a complex which is composed of at least 5 members: HTATSF1/Tat-SF1, P-TEFb complex, RNA pol II, SUPT5H, and NCL/nucleolin. Associates with UBR5 and forms a transcription regulatory complex composed of CDK9, RNAP II, UBR5 and TFIIS/TCEA1 that can stimulate target gene transcription (e.g. gamma fibrinogen/FGG) by recruiting their promoters.
Component of the 7SK snRNP inactive complex which is composed of at least 8 members: P-TEFb (composed of CDK9 and CCNT1/cyclin-T1), HEXIM1, HEXIM2, LARP7, BCDIN3, SART3 proteins and 7SK and U6 snRNAs. This inactive 7SK snRNP complex can also interact with NCOR1 and HDAC3, probably to regulate CDK9 acetylation. Release of P-TEFb from P-TEFb/7SK snRNP complex requires both PP2B to transduce calcium Ca2+ signaling in response to stimuli (e.g. UV or hexamethylene bisacetamide (HMBA)), and PPP1CA to dephosphorylate Thr-186. This released P-TEFb remains inactive in the pre-initiation complex with BRD4 until new Thr-186 phosphorylation occurs after the synthesis of a short RNA.
Interacts with BRD4; to target chromatin binding.
Interacts with JMJD6.
Interacts with activated nuclear STAT3 and RELA/p65. Binds to AR and MYOD1.
Forms a complex composed of CDK9, CCNT1/cyclin-T1, EP300 and GATA4 that stimulates hypertrophy in cardiomyocytes (By similarity). The large PER complex involved in the repression of transcriptional termination is composed of at least PER2, CDK9, DDX5, DHX9, NCBP1 and POLR2A (active) (PubMed:22767893).
Interacts with HSF1 (By similarity).
Interacts with TBX21 (PubMed:27292648).
Interacts with WDR43 (PubMed:31128943).
By similarity3 PublicationsBinary interactionsi
Hide detailsQ99J95
With | #Exp. | IntAct |
---|---|---|
Brdt [Q91Y44] | 3 | EBI-2654963,EBI-6260929 |
Max [P28574] | 2 | EBI-2654963,EBI-1183003 |
Srsf2 [Q62093] | 3 | EBI-2654963,EBI-2550402 |
GO - Molecular functioni
- protein kinase binding Source: MGI
- transcription coactivator binding Source: MGI
- transcription factor binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 223714, 8 interactors |
ComplexPortali | CPX-230, Positive transcription elongation factor B, CDK9-cyclinT1 complex CPX-323, Positive transcription elongation factor B, CDK9-cyclinT2a complex CPX-324, Positive transcription elongation factor B, CDK9-cyclinT2b complex |
CORUMi | Q99J95 |
DIPi | DIP-46368N |
IntActi | Q99J95, 104 interactors |
MINTi | Q99J95 |
STRINGi | 10090.ENSMUSP00000009699 |
Miscellaneous databases
RNActi | Q99J95, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 19 – 315 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 297 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 166 – 191 | T-loopBy similarityAdd BLAST | 26 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0669, Eukaryota |
GeneTreei | ENSGT00940000155373 |
HOGENOMi | CLU_000288_181_1_1 |
InParanoidi | Q99J95 |
OMAi | KDPTGCD |
OrthoDBi | 925637at2759 |
PhylomeDBi | Q99J95 |
TreeFami | TF101039 |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAKQYDSVEC PFCDEVTKYE KLAKIGQGTF GEVFKAKHRQ TGQKVALKKV
60 70 80 90 100
LMENEKEGFP ITALREIKIL QLLKHENVVN LIEICRTKAS PYNRCKGSIY
110 120 130 140 150
LVFDFCEHDL AGLLSNVLVK FTLSEIKRVM QMLLNGLYYI HRNKILHRDM
160 170 180 190 200
KAANVLITRD GVLKLADFGL ARAFSLAKNS QPNRYTNRVV TLWYRPPELL
210 220 230 240 250
LGERDYGPPI DLWGAGCIMA EMWTRSPIMQ GNTEQHQLAL ISQLCGSITP
260 270 280 290 300
EVWPNVDKYE LFEKLELVKG QKRKVKDRLK AYVRDPYALD LIDKLLVLDP
310 320 330 340 350
AQRIDSDDAL NHDFFWSDPM PSDLKGMLST HLTSMFEYLA PPRRKGSQIT
360 370
QQSTNQSRNP ATTNQTEFER VF
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketD6RHP4 | D6RHP4_MOUSE | Cyclin-dependent kinase 9 | Cdk9 | 61 | Annotation score: | ||
B0R021 | B0R021_MOUSE | Cyclin-dependent kinase 9 | Cdk9 | 54 | Annotation score: | ||
D6RGN3 | D6RGN3_MOUSE | Cyclin-dependent kinase 9 | Cdk9 | 36 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 122 | T → R in BAE34054 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 154 | N → S in BAE25055 (PubMed:16141072).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_016290 | 1 – 129 | Missing in isoform 3. 1 PublicationAdd BLAST | 129 | |
Alternative sequenceiVSP_016289 | 1 – 51 | Missing in isoform 2. 1 PublicationAdd BLAST | 51 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF327431 mRNA Translation: AAK15699.1 AF327569 Genomic DNA Translation: AAK15706.1 AK089276 mRNA Translation: BAC40824.1 AK142397 mRNA Translation: BAE25055.1 AK143217 mRNA Translation: BAE25312.1 AK144607 mRNA Translation: BAE25966.1 Frameshift. AK157340 mRNA Translation: BAE34054.1 AL772271 Genomic DNA No translation available. BC003901 mRNA Translation: AAH03901.1 |
CCDSi | CCDS15927.1 [Q99J95-1] |
RefSeqi | NP_570930.1, NM_130860.3 [Q99J95-1] XP_011237303.1, XM_011239001.1 [Q99J95-3] |
Genome annotation databases
Ensembli | ENSMUST00000009699; ENSMUSP00000009699; ENSMUSG00000009555 [Q99J95-1] ENSMUST00000120105; ENSMUSP00000113327; ENSMUSG00000009555 [Q99J95-2] |
GeneIDi | 107951 |
KEGGi | mmu:107951 |
UCSCi | uc008jgn.2, mouse [Q99J95-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF327431 mRNA Translation: AAK15699.1 AF327569 Genomic DNA Translation: AAK15706.1 AK089276 mRNA Translation: BAC40824.1 AK142397 mRNA Translation: BAE25055.1 AK143217 mRNA Translation: BAE25312.1 AK144607 mRNA Translation: BAE25966.1 Frameshift. AK157340 mRNA Translation: BAE34054.1 AL772271 Genomic DNA No translation available. BC003901 mRNA Translation: AAH03901.1 |
CCDSi | CCDS15927.1 [Q99J95-1] |
RefSeqi | NP_570930.1, NM_130860.3 [Q99J95-1] XP_011237303.1, XM_011239001.1 [Q99J95-3] |
3D structure databases
SMRi | Q99J95 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 223714, 8 interactors |
ComplexPortali | CPX-230, Positive transcription elongation factor B, CDK9-cyclinT1 complex CPX-323, Positive transcription elongation factor B, CDK9-cyclinT2a complex CPX-324, Positive transcription elongation factor B, CDK9-cyclinT2b complex |
CORUMi | Q99J95 |
DIPi | DIP-46368N |
IntActi | Q99J95, 104 interactors |
MINTi | Q99J95 |
STRINGi | 10090.ENSMUSP00000009699 |
Chemistry databases
ChEMBLi | CHEMBL4105875 |
PTM databases
iPTMneti | Q99J95 |
PhosphoSitePlusi | Q99J95 |
Proteomic databases
EPDi | Q99J95 |
jPOSTi | Q99J95 |
PaxDbi | Q99J95 |
PeptideAtlasi | Q99J95 |
PRIDEi | Q99J95 |
ProteomicsDBi | 280041 [Q99J95-1] 280042 [Q99J95-2] 280043 [Q99J95-3] |
Protocols and materials databases
Antibodypediai | 1447, 553 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000009699; ENSMUSP00000009699; ENSMUSG00000009555 [Q99J95-1] ENSMUST00000120105; ENSMUSP00000113327; ENSMUSG00000009555 [Q99J95-2] |
GeneIDi | 107951 |
KEGGi | mmu:107951 |
UCSCi | uc008jgn.2, mouse [Q99J95-1] |
Organism-specific databases
CTDi | 1025 |
MGIi | MGI:1328368, Cdk9 |
Phylogenomic databases
eggNOGi | KOG0669, Eukaryota |
GeneTreei | ENSGT00940000155373 |
HOGENOMi | CLU_000288_181_1_1 |
InParanoidi | Q99J95 |
OMAi | KDPTGCD |
OrthoDBi | 925637at2759 |
PhylomeDBi | Q99J95 |
TreeFami | TF101039 |
Enzyme and pathway databases
Reactomei | R-MMU-112382, Formation of RNA Pol II elongation complex R-MMU-2173796, SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription R-MMU-674695, RNA Polymerase II Pre-transcription Events R-MMU-6796648, TP53 Regulates Transcription of DNA Repair Genes R-MMU-6807505, RNA polymerase II transcribes snRNA genes R-MMU-75955, RNA Polymerase II Transcription Elongation R-MMU-9018519, Estrogen-dependent gene expression |
Miscellaneous databases
BioGRID-ORCSi | 107951, 24 hits in 56 CRISPR screens |
ChiTaRSi | Cdk9, mouse |
PROi | PR:Q99J95 |
RNActi | Q99J95, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000009555, Expressed in granulocyte and 314 other tissues |
ExpressionAtlasi | Q99J95, baseline and differential |
Genevisiblei | Q99J95, MM |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CDK9_MOUSE | |
Accessioni | Q99J95Primary (citable) accession number: Q99J95 Secondary accession number(s): B0R020 Q8BTN0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 2005 |
Last sequence update: | June 1, 2001 | |
Last modified: | April 7, 2021 | |
This is version 172 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human and mouse protein kinases
Human and mouse protein kinases: classification and index - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families