UniProtKB - Q99999 (G3ST1_HUMAN)
Galactosylceramide sulfotransferase
GAL3ST1
Functioni
Catalyzes the transfer of a sulfate group to position 3 of non-reducing beta-galactosyl residues in glycerolipids and sphingolipids, therefore participates in the biosynthesis of sulfoglycolipids (PubMed:9030544, PubMed:8830034).
Catalyzes the synthesis of galactosylceramide sulfate (sulfatide), a major lipid component of the myelin sheath and of monogalactosylalkylacylglycerol sulfate (seminolipid), present in spermatocytes (PubMed:8830034).
Seems to prefer beta-glycosides at the non-reducing termini of sugar chains attached to a lipid moiety (PubMed:8830034).
Also acts on lactosylceramide, galactosyl 1-alkyl-2-sn-glycerol and galactosyl diacylglycerol (in vitro) (PubMed:8830034).
2 PublicationsCatalytic activityi
- 3'-phosphoadenylyl sulfate + a β-D-galactosyl-(1↔1ʼ)-N-acylsphing-4-enine = adenosine 3',5'-bisphosphate + H+ + N-acyl-1-β-D-(3-O-sulfo)-galactosyl-sphing-4-enine1 PublicationEC:2.8.2.111 PublicationThis reaction proceeds in the forward1 Publication direction.
- 3'-phosphoadenylyl sulfate + a 1-O-alkyl-2-acyl-3-O-(β-D-galactosyl)-sn-glycerol = a 1-O-alkyl-2-acyl-3-(β-D-3-sulfogalactosyl)-sn-glycerol + adenosine 3',5'-bisphosphate + H+1 PublicationEC:2.8.2.111 PublicationThis reaction proceeds in the forward1 Publication direction.
- 3'-phosphoadenylyl sulfate + β-D-Gal-(1↔1ʼ)-Cer = 1-(3-O-sulfo-β-D-galactosyl)-ceramide + adenosine 3',5'-bisphosphate + H+1 PublicationThis reaction proceeds in the forward1 Publication direction.
- 3'-phosphoadenylyl sulfate + a 1,2-diacyl-3-O-(β-D-galactosyl)-sn-glycerol = 1,2-diacyl-3-(3-O-sulfo-β-D-galactosyl)-sn-glycerol + adenosine 3',5'-bisphosphate + H+1 PublicationThis reaction proceeds in the forward1 Publication direction.
- 3'-phosphoadenylyl sulfate + a β-D-Gal-(1→4)-β-D-Glc-(1↔1)-Cer(d18:1(4E)) = adenosine 3',5'-bisphosphate + β-D-3-sulfogalactosyl-(1→4)-β-D-glucosyl-(1↔1ʼ)-N-acylsphing-4-enine + H+1 PublicationThis reaction proceeds in the forward1 Publication direction.
: sphingolipid metabolism Pathwayi
This protein is involved in the pathway sphingolipid metabolism, which is part of Lipid metabolism.1 PublicationView all proteins of this organism that are known to be involved in the pathway sphingolipid metabolism and in Lipid metabolism.
GO - Molecular functioni
- galactose 3-O-sulfotransferase activity Source: GO_Central
- galactosylceramide sulfotransferase activity Source: UniProtKB
- sulfotransferase activity Source: GO_Central
GO - Biological processi
- galactosylceramide biosynthetic process Source: GO_Central
- galactosylceramide metabolic process Source: UniProtKB
- glycerolipid metabolic process Source: UniProtKB
- myelination Source: GO_Central
- protein N-linked glycosylation Source: ProtInc
- spermatogenesis Source: Ensembl
- sphingolipid metabolic process Source: UniProtKB
Keywordsi
Molecular function | Transferase |
Biological process | Lipid metabolism, Sphingolipid metabolism |
Enzyme and pathway databases
BRENDAi | 2.8.2.11, 2681 |
PathwayCommonsi | Q99999 |
SignaLinki | Q99999 |
UniPathwayi | UPA00222 |
Chemistry databases
SwissLipidsi | SLP:000000746 |
Names & Taxonomyi
Protein namesi | Recommended name: Galactosylceramide sulfotransferaseCurated (EC:2.8.2.111 Publication)Short name: GalCer sulfotransferase1 Publication Alternative name(s): 3'-phosphoadenosine-5'-phosphosulfate:GalCer sulfotransferase1 Publication 3'-phosphoadenylylsulfate:galactosylceramide 3'-sulfotransferase1 Publication Cerebroside sulfotransferaseBy similarity |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:24240, GAL3ST1 |
MIMi | 602300, gene |
neXtProti | NX_Q99999 |
VEuPathDBi | HostDB:ENSG00000128242 |
Subcellular locationi
Golgi apparatus
- Golgi apparatus membrane Curated; Single-pass type II membrane protein Curated
Golgi apparatus
- Golgi membrane Source: UniProtKB-SubCell
Plasma Membrane
- integral component of plasma membrane Source: ProtInc
Other locations
- membrane Source: ProtInc
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 14 | CytoplasmicSequence analysisAdd BLAST | 14 | |
Transmembranei | 15 – 35 | Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST | 21 | |
Topological domaini | 36 – 423 | LumenalSequence analysisAdd BLAST | 388 |
Keywords - Cellular componenti
Golgi apparatus, MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 9514 |
OpenTargetsi | ENSG00000128242 |
PharmGKBi | PA134889661 |
Miscellaneous databases
Pharosi | Q99999, Tbio |
Genetic variation databases
BioMutai | GAL3ST1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000085201 | 1 – 423 | Galactosylceramide sulfotransferaseAdd BLAST | 423 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 66 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 312 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
GlycoproteinProteomic databases
MassIVEi | Q99999 |
MaxQBi | Q99999 |
PaxDbi | Q99999 |
PeptideAtlasi | Q99999 |
PRIDEi | Q99999 |
ProteomicsDBi | 78572 |
PTM databases
GlyGeni | Q99999, 5 sites, 2 O-linked glycans (3 sites) |
iPTMneti | Q99999 |
PhosphoSitePlusi | Q99999 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000128242, Expressed in C1 segment of cervical spinal cord and 147 other tissues |
ExpressionAtlasi | Q99999, baseline and differential |
Genevisiblei | Q99999, HS |
Organism-specific databases
HPAi | ENSG00000128242, Tissue enhanced (brain, intestine) |
Interactioni
Protein-protein interaction databases
BioGRIDi | 114891, 24 interactors |
IntActi | Q99999, 12 interactors |
MINTi | Q99999 |
STRINGi | 9606.ENSP00000385735 |
Miscellaneous databases
RNActi | Q99999, protein |
Structurei
3D structure databases
AlphaFoldDBi | Q99999 |
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502QTXT, Eukaryota |
GeneTreei | ENSGT00950000182923 |
HOGENOMi | CLU_040616_1_1_1 |
InParanoidi | Q99999 |
OMAi | FHYFGRV |
OrthoDBi | 1385827at2759 |
PhylomeDBi | Q99999 |
TreeFami | TF314802 |
Family and domain databases
Gene3Di | 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR009729, Gal-3-0_sulfotransfrase IPR027417, P-loop_NTPase |
PANTHERi | PTHR14647, PTHR14647, 1 hit |
Pfami | View protein in Pfam PF06990, Gal-3-0_sulfotr, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 16 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MLPPQKKPWE SMAKGLVLGA LFTSFLLLVY SYAVPPLHAG LASTTPEAAA
60 70 80 90 100
SCSPPALEPE AVIRANGSAG ECQPRRNIVF LKTHKTASST LLNILFRFGQ
110 120 130 140 150
KHRLKFAFPN GRNDFDYPTF FARSLVQDYR PGACFNIICN HMRFHYDEVR
160 170 180 190 200
GLVPTNAIFI TVLRDPARLF ESSFHYFGPV VPLTWKLSAG DKLTEFLQDP
210 220 230 240 250
DRYYDPNGFN AHYLRNLLFF DLGYDNSLDP SSPQVQEHIL EVERRFHLVL
260 270 280 290 300
LQEYFDESLV LLKDLLCWEL EDVLYFKLNA RRDSPVPRLS GELYGRATAW
310 320 330 340 350
NMLDSHLYRH FNASFWRKVE AFGRERMARE VAALRHANER MRTICIDGGH
360 370 380 390 400
AVDAAAIQDE AMQPWQPLGT KSILGYNLKK SIGQRHAQLC RRMLTPEIQY
410 420
LMDLGANLWV TKLWKFIRDF LRW
Computationally mapped potential isoform sequencesi
There are 16 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketC9JGL4 | C9JGL4_HUMAN | Galactosylceramide sulfotransferase | GAL3ST1 | 279 | Annotation score: | ||
C9J6M2 | C9J6M2_HUMAN | Galactosylceramide sulfotransferase | GAL3ST1 | 242 | Annotation score: | ||
C9JE31 | C9JE31_HUMAN | Galactosylceramide sulfotransferase | GAL3ST1 | 265 | Annotation score: | ||
C9J2X7 | C9J2X7_HUMAN | Galactosylceramide sulfotransferase | GAL3ST1 | 170 | Annotation score: | ||
C9J993 | C9J993_HUMAN | Galactosylceramide sulfotransferase | GAL3ST1 | 149 | Annotation score: | ||
C9JTV3 | C9JTV3_HUMAN | Galactosylceramide sulfotransferase | GAL3ST1 | 112 | Annotation score: | ||
C9JYD7 | C9JYD7_HUMAN | Galactosylceramide sulfotransferase | GAL3ST1 | 199 | Annotation score: | ||
C9J2M1 | C9J2M1_HUMAN | Galactosylceramide sulfotransferase | GAL3ST1 | 143 | Annotation score: | ||
C9J3S3 | C9J3S3_HUMAN | Galactosylceramide sulfotransferase | GAL3ST1 | 163 | Annotation score: | ||
C9JLB5 | C9JLB5_HUMAN | Galactosylceramide sulfotransferase | GAL3ST1 | 102 | Annotation score: | ||
There are more potential isoformsShow all |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 281 – 282 | RR → LN AA sequence (PubMed:9030544).Curated | 2 | |
Sequence conflicti | 418 | R → L AA sequence (PubMed:9030544).Curated | 1 | |
Sequence conflicti | 422 | R → E AA sequence (PubMed:9030544).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_013684 | 29 | V → M2 PublicationsCorresponds to variant dbSNP:rs2267161Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D88667 mRNA Translation: BAA13673.1 AB029901 Genomic DNA Translation: BAA89503.1 CR456350 mRNA Translation: CAG30236.1 BC014649 mRNA Translation: AAH14649.1 BC019077 mRNA Translation: AAH19077.1 |
CCDSi | CCDS13879.1 |
RefSeqi | NP_001305032.1, NM_001318103.1 NP_001305033.1, NM_001318104.1 NP_001305034.1, NM_001318105.1 NP_001305035.1, NM_001318106.1 NP_001305036.1, NM_001318107.1 NP_001305037.1, NM_001318108.1 NP_001305038.1, NM_001318109.1 NP_001305039.1, NM_001318110.1 NP_001305040.1, NM_001318111.1 NP_001305041.1, NM_001318112.1 NP_001305042.1, NM_001318113.1 NP_001305043.1, NM_001318114.1 NP_001305044.1, NM_001318115.1 NP_001305045.1, NM_001318116.1 NP_004852.1, NM_004861.2 XP_016884587.1, XM_017029098.1 |
Genome annotation databases
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D88667 mRNA Translation: BAA13673.1 AB029901 Genomic DNA Translation: BAA89503.1 CR456350 mRNA Translation: CAG30236.1 BC014649 mRNA Translation: AAH14649.1 BC019077 mRNA Translation: AAH19077.1 |
CCDSi | CCDS13879.1 |
RefSeqi | NP_001305032.1, NM_001318103.1 NP_001305033.1, NM_001318104.1 NP_001305034.1, NM_001318105.1 NP_001305035.1, NM_001318106.1 NP_001305036.1, NM_001318107.1 NP_001305037.1, NM_001318108.1 NP_001305038.1, NM_001318109.1 NP_001305039.1, NM_001318110.1 NP_001305040.1, NM_001318111.1 NP_001305041.1, NM_001318112.1 NP_001305042.1, NM_001318113.1 NP_001305043.1, NM_001318114.1 NP_001305044.1, NM_001318115.1 NP_001305045.1, NM_001318116.1 NP_004852.1, NM_004861.2 XP_016884587.1, XM_017029098.1 |
3D structure databases
AlphaFoldDBi | Q99999 |
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 114891, 24 interactors |
IntActi | Q99999, 12 interactors |
MINTi | Q99999 |
STRINGi | 9606.ENSP00000385735 |
Chemistry databases
SwissLipidsi | SLP:000000746 |
PTM databases
GlyGeni | Q99999, 5 sites, 2 O-linked glycans (3 sites) |
iPTMneti | Q99999 |
PhosphoSitePlusi | Q99999 |
Genetic variation databases
BioMutai | GAL3ST1 |
Proteomic databases
MassIVEi | Q99999 |
MaxQBi | Q99999 |
PaxDbi | Q99999 |
PeptideAtlasi | Q99999 |
PRIDEi | Q99999 |
ProteomicsDBi | 78572 |
Protocols and materials databases
Antibodypediai | 243, 144 antibodies from 20 providers |
DNASUi | 9514 |
Genome annotation databases
Organism-specific databases
CTDi | 9514 |
DisGeNETi | 9514 |
GeneCardsi | GAL3ST1 |
HGNCi | HGNC:24240, GAL3ST1 |
HPAi | ENSG00000128242, Tissue enhanced (brain, intestine) |
MIMi | 602300, gene |
neXtProti | NX_Q99999 |
OpenTargetsi | ENSG00000128242 |
PharmGKBi | PA134889661 |
VEuPathDBi | HostDB:ENSG00000128242 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502QTXT, Eukaryota |
GeneTreei | ENSGT00950000182923 |
HOGENOMi | CLU_040616_1_1_1 |
InParanoidi | Q99999 |
OMAi | FHYFGRV |
OrthoDBi | 1385827at2759 |
PhylomeDBi | Q99999 |
TreeFami | TF314802 |
Enzyme and pathway databases
UniPathwayi | UPA00222 |
BRENDAi | 2.8.2.11, 2681 |
PathwayCommonsi | Q99999 |
SignaLinki | Q99999 |
Miscellaneous databases
BioGRID-ORCSi | 9514, 12 hits in 1061 CRISPR screens |
GeneWikii | GAL3ST1 |
GenomeRNAii | 9514 |
Pharosi | Q99999, Tbio |
PROi | PR:Q99999 |
RNActi | Q99999, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000128242, Expressed in C1 segment of cervical spinal cord and 147 other tissues |
ExpressionAtlasi | Q99999, baseline and differential |
Genevisiblei | Q99999, HS |
Family and domain databases
Gene3Di | 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR009729, Gal-3-0_sulfotransfrase IPR027417, P-loop_NTPase |
PANTHERi | PTHR14647, PTHR14647, 1 hit |
Pfami | View protein in Pfam PF06990, Gal-3-0_sulfotr, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | G3ST1_HUMAN | |
Accessioni | Q99999Primary (citable) accession number: Q99999 Secondary accession number(s): Q96C63 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 26, 2002 |
Last sequence update: | May 1, 1997 | |
Last modified: | May 25, 2022 | |
This is version 168 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 22
Human chromosome 22: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families