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Protein

Galactosylceramide sulfotransferase

Gene

GAL3ST1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the sulfation of membrane glycolipids. Seems to prefer beta-glycosides at the non-reducing termini of sugar chains attached to a lipid moiety. Catalyzes the synthesis of galactosylceramide sulfate (sulfatide), a major lipid component of the myelin sheath and of monogalactosylalkylacylglycerol sulfate (seminolipid), present in spermatocytes (By similarity). Also acts on lactosylceramide, galactosyl 1-alkyl-2-sn-glycerol and galactosyl diacylglycerol (in vitro).By similarity1 Publication

Catalytic activityi

3'-phosphoadenylyl sulfate + a galactosylceramide = adenosine 3',5'-bisphosphate + a galactosylceramidesulfate.
3'-phosphoadenylyl sulfate + monogalactosylalkylacylglycerol = adenosine 3',5'-bisphosphate + monogalactosylalkylacylglycerol sulfate.

Pathwayi: sphingolipid metabolism

This protein is involved in the pathway sphingolipid metabolism, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway sphingolipid metabolism and in Lipid metabolism.

GO - Molecular functioni

  • galactosylceramide sulfotransferase activity Source: UniProtKB-EC
  • sulfotransferase activity Source: ProtInc

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processLipid metabolism, Sphingolipid metabolism

Enzyme and pathway databases

BioCyciMetaCyc:HS05164-MONOMER
BRENDAi2.8.2.11 2681
UniPathwayi
UPA00222

Chemistry databases

SwissLipidsiSLP:000000746

Names & Taxonomyi

Protein namesi
Recommended name:
Galactosylceramide sulfotransferase (EC:2.8.2.11)
Short name:
GalCer sulfotransferase
Alternative name(s):
3'-phosphoadenosine-5'-phosphosulfate:GalCer sulfotransferase
3'-phosphoadenylylsulfate:galactosylceramide 3'-sulfotransferase
Cerebroside sulfotransferase
Gene namesi
Name:GAL3ST1
Synonyms:CST
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 22

Organism-specific databases

EuPathDBiHostDB:ENSG00000128242.12
HGNCiHGNC:24240 GAL3ST1
MIMi602300 gene
neXtProtiNX_Q99999

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 14CytoplasmicSequence analysisAdd BLAST14
Transmembranei15 – 35Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini36 – 423LumenalSequence analysisAdd BLAST388

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi9514
OpenTargetsiENSG00000128242
PharmGKBiPA134889661

Polymorphism and mutation databases

BioMutaiGAL3ST1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000852011 – 423Galactosylceramide sulfotransferaseAdd BLAST423

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi66N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi312N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ99999
PaxDbiQ99999
PeptideAtlasiQ99999
PRIDEiQ99999
ProteomicsDBi78572

PTM databases

iPTMnetiQ99999
PhosphoSitePlusiQ99999

Expressioni

Tissue specificityi

Expressed in kidney proximal tubule, gastric mucosa and adenocarcinoma. Highly expressed in renal cell carcinoma cell lines.

Gene expression databases

BgeeiENSG00000128242 Expressed in 128 organ(s), highest expression level in C1 segment of cervical spinal cord
CleanExiHS_GAL3ST1
ExpressionAtlasiQ99999 baseline and differential
GenevisibleiQ99999 HS

Organism-specific databases

HPAiHPA001220

Interactioni

Protein-protein interaction databases

BioGridi114891, 9 interactors
IntActiQ99999, 1 interactor
MINTiQ99999
STRINGi9606.ENSP00000343234

Structurei

3D structure databases

ProteinModelPortaliQ99999
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IG5S Eukaryota
ENOG4110RXY LUCA
GeneTreeiENSGT00390000013505
HOGENOMiHOG000232148
HOVERGENiHBG051681
InParanoidiQ99999
KOiK01019
OMAiIQDSAMQ
OrthoDBiEOG091G0N28
PhylomeDBiQ99999
TreeFamiTF314802

Family and domain databases

InterProiView protein in InterPro
IPR009729 Gal-3-0_sulfotransfrase
IPR027417 P-loop_NTPase
PANTHERiPTHR14647 PTHR14647, 1 hit
PfamiView protein in Pfam
PF06990 Gal-3-0_sulfotr, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 16 potential isoforms that are computationally mapped.Show allAlign All

Q99999-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLPPQKKPWE SMAKGLVLGA LFTSFLLLVY SYAVPPLHAG LASTTPEAAA
60 70 80 90 100
SCSPPALEPE AVIRANGSAG ECQPRRNIVF LKTHKTASST LLNILFRFGQ
110 120 130 140 150
KHRLKFAFPN GRNDFDYPTF FARSLVQDYR PGACFNIICN HMRFHYDEVR
160 170 180 190 200
GLVPTNAIFI TVLRDPARLF ESSFHYFGPV VPLTWKLSAG DKLTEFLQDP
210 220 230 240 250
DRYYDPNGFN AHYLRNLLFF DLGYDNSLDP SSPQVQEHIL EVERRFHLVL
260 270 280 290 300
LQEYFDESLV LLKDLLCWEL EDVLYFKLNA RRDSPVPRLS GELYGRATAW
310 320 330 340 350
NMLDSHLYRH FNASFWRKVE AFGRERMARE VAALRHANER MRTICIDGGH
360 370 380 390 400
AVDAAAIQDE AMQPWQPLGT KSILGYNLKK SIGQRHAQLC RRMLTPEIQY
410 420
LMDLGANLWV TKLWKFIRDF LRW
Length:423
Mass (Da):48,764
Last modified:May 1, 1997 - v1
Checksum:i0FD4F2E374447C56
GO

Computationally mapped potential isoform sequencesi

There are 16 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
C9JGL4C9JGL4_HUMAN
Galactosylceramide sulfotransferase
GAL3ST1
279Annotation score:
C9JE31C9JE31_HUMAN
Galactosylceramide sulfotransferase
GAL3ST1
265Annotation score:
C9J6M2C9J6M2_HUMAN
Galactosylceramide sulfotransferase
GAL3ST1
242Annotation score:
C9JYD7C9JYD7_HUMAN
Galactosylceramide sulfotransferase
GAL3ST1
199Annotation score:
C9J3S3C9J3S3_HUMAN
Galactosylceramide sulfotransferase
GAL3ST1
163Annotation score:
C9JTV3C9JTV3_HUMAN
Galactosylceramide sulfotransferase
GAL3ST1
112Annotation score:
C9J2X7C9J2X7_HUMAN
Galactosylceramide sulfotransferase
GAL3ST1
170Annotation score:
C9JKD7C9JKD7_HUMAN
Galactosylceramide sulfotransferase
GAL3ST1
98Annotation score:
C9J993C9J993_HUMAN
Galactosylceramide sulfotransferase
GAL3ST1
149Annotation score:
C9J2M1C9J2M1_HUMAN
Galactosylceramide sulfotransferase
GAL3ST1
143Annotation score:
There are more potential isoformsShow all

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti281 – 282RR → LN AA sequence (PubMed:9030544).Curated2
Sequence conflicti418R → L AA sequence (PubMed:9030544).Curated1
Sequence conflicti422R → E AA sequence (PubMed:9030544).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_01368429V → M2 PublicationsCorresponds to variant dbSNP:rs2267161Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D88667 mRNA Translation: BAA13673.1
AB029901 Genomic DNA Translation: BAA89503.1
CR456350 mRNA Translation: CAG30236.1
BC014649 mRNA Translation: AAH14649.1
BC019077 mRNA Translation: AAH19077.1
CCDSiCCDS13879.1
RefSeqiNP_001305032.1, NM_001318103.1
NP_001305033.1, NM_001318104.1
NP_001305034.1, NM_001318105.1
NP_001305035.1, NM_001318106.1
NP_001305036.1, NM_001318107.1
NP_001305037.1, NM_001318108.1
NP_001305038.1, NM_001318109.1
NP_001305039.1, NM_001318110.1
NP_001305040.1, NM_001318111.1
NP_001305041.1, NM_001318112.1
NP_001305042.1, NM_001318113.1
NP_001305043.1, NM_001318114.1
NP_001305044.1, NM_001318115.1
NP_001305045.1, NM_001318116.1
NP_004852.1, NM_004861.2
XP_016884587.1, XM_017029098.1
UniGeneiHs.17958

Genome annotation databases

EnsembliENST00000338911; ENSP00000343234; ENSG00000128242
ENST00000401975; ENSP00000384388; ENSG00000128242
ENST00000402321; ENSP00000385735; ENSG00000128242
ENST00000402369; ENSP00000384122; ENSG00000128242
ENST00000406361; ENSP00000385207; ENSG00000128242
ENST00000406955; ENSP00000385825; ENSG00000128242
GeneIDi9514
KEGGihsa:9514
UCSCiuc003aih.2 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D88667 mRNA Translation: BAA13673.1
AB029901 Genomic DNA Translation: BAA89503.1
CR456350 mRNA Translation: CAG30236.1
BC014649 mRNA Translation: AAH14649.1
BC019077 mRNA Translation: AAH19077.1
CCDSiCCDS13879.1
RefSeqiNP_001305032.1, NM_001318103.1
NP_001305033.1, NM_001318104.1
NP_001305034.1, NM_001318105.1
NP_001305035.1, NM_001318106.1
NP_001305036.1, NM_001318107.1
NP_001305037.1, NM_001318108.1
NP_001305038.1, NM_001318109.1
NP_001305039.1, NM_001318110.1
NP_001305040.1, NM_001318111.1
NP_001305041.1, NM_001318112.1
NP_001305042.1, NM_001318113.1
NP_001305043.1, NM_001318114.1
NP_001305044.1, NM_001318115.1
NP_001305045.1, NM_001318116.1
NP_004852.1, NM_004861.2
XP_016884587.1, XM_017029098.1
UniGeneiHs.17958

3D structure databases

ProteinModelPortaliQ99999
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114891, 9 interactors
IntActiQ99999, 1 interactor
MINTiQ99999
STRINGi9606.ENSP00000343234

Chemistry databases

SwissLipidsiSLP:000000746

PTM databases

iPTMnetiQ99999
PhosphoSitePlusiQ99999

Polymorphism and mutation databases

BioMutaiGAL3ST1

Proteomic databases

MaxQBiQ99999
PaxDbiQ99999
PeptideAtlasiQ99999
PRIDEiQ99999
ProteomicsDBi78572

Protocols and materials databases

DNASUi9514
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000338911; ENSP00000343234; ENSG00000128242
ENST00000401975; ENSP00000384388; ENSG00000128242
ENST00000402321; ENSP00000385735; ENSG00000128242
ENST00000402369; ENSP00000384122; ENSG00000128242
ENST00000406361; ENSP00000385207; ENSG00000128242
ENST00000406955; ENSP00000385825; ENSG00000128242
GeneIDi9514
KEGGihsa:9514
UCSCiuc003aih.2 human

Organism-specific databases

CTDi9514
DisGeNETi9514
EuPathDBiHostDB:ENSG00000128242.12
GeneCardsiGAL3ST1
HGNCiHGNC:24240 GAL3ST1
HPAiHPA001220
MIMi602300 gene
neXtProtiNX_Q99999
OpenTargetsiENSG00000128242
PharmGKBiPA134889661
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IG5S Eukaryota
ENOG4110RXY LUCA
GeneTreeiENSGT00390000013505
HOGENOMiHOG000232148
HOVERGENiHBG051681
InParanoidiQ99999
KOiK01019
OMAiIQDSAMQ
OrthoDBiEOG091G0N28
PhylomeDBiQ99999
TreeFamiTF314802

Enzyme and pathway databases

UniPathwayi
UPA00222

BioCyciMetaCyc:HS05164-MONOMER
BRENDAi2.8.2.11 2681

Miscellaneous databases

GeneWikiiGAL3ST1
GenomeRNAii9514
PROiPR:Q99999
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000128242 Expressed in 128 organ(s), highest expression level in C1 segment of cervical spinal cord
CleanExiHS_GAL3ST1
ExpressionAtlasiQ99999 baseline and differential
GenevisibleiQ99999 HS

Family and domain databases

InterProiView protein in InterPro
IPR009729 Gal-3-0_sulfotransfrase
IPR027417 P-loop_NTPase
PANTHERiPTHR14647 PTHR14647, 1 hit
PfamiView protein in Pfam
PF06990 Gal-3-0_sulfotr, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiG3ST1_HUMAN
AccessioniPrimary (citable) accession number: Q99999
Secondary accession number(s): Q96C63
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 26, 2002
Last sequence update: May 1, 1997
Last modified: November 7, 2018
This is version 148 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. Human chromosome 22
    Human chromosome 22: entries, gene names and cross-references to MIM
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Main funding by: National Institutes of Health

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