UniProtKB - Q99986 (VRK1_HUMAN)
Serine/threonine-protein kinase VRK1
VRK1
Functioni
Serine/threonine kinase involved in cell cycle, nuclear condensation and transcription regulation (PubMed:14645249, PubMed:18617507, PubMed:19103756).
Involved in Golgi disassembly during the cell cycle: following phosphorylation by PLK3 during mitosis, required to induce Golgi fragmentation (PubMed:19103756).
Phosphorylates 'Thr-18' of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2 (PubMed:10951572).
Phosphorylates KAT5 in response to DNA damage, promoting KAT5 association with chromatin and histone acetyltransferase activity (PubMed:33076429).
Phosphorylates BANF1: disrupts its ability to bind DNA, reduces its binding to LEM domain-containing proteins and causes its relocalization from the nucleus to the cytoplasm (PubMed:16495336).
Phosphorylates ATF2 which activates its transcriptional activity (PubMed:15105425).
7 PublicationsCatalytic activityi
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 71 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 177 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 43 – 51 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- histone binding Source: GO_Central
- histone kinase activity (H3-S10 specific) Source: UniProtKB
- histone kinase activity (H3-T3 specific) Source: UniProtKB
- protein kinase activity Source: UniProtKB
- protein kinase binding Source: MGI
- protein serine/threonine/tyrosine kinase activity Source: RHEA
- protein serine/threonine kinase activity Source: UniProtKB
- protein serine kinase activity Source: RHEA
GO - Biological processi
- cell division Source: UniProtKB-KW
- cellular response to DNA damage stimulus Source: UniProtKB
- Golgi disassembly Source: UniProtKB
- histone phosphorylation Source: GO_Central
- mitotic nuclear membrane disassembly Source: Reactome
- peptidyl-serine phosphorylation Source: GO_Central
- positive regulation of protein localization to chromatin Source: UniProtKB
- protein autophosphorylation Source: UniProtKB
- protein phosphorylation Source: ProtInc
- signal transduction Source: GO_Central
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Biological process | Cell cycle, Cell division, Host-virus interaction, Mitosis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
PathwayCommonsi | Q99986 |
Reactomei | R-HSA-2980766, Nuclear Envelope Breakdown R-HSA-2995383, Initiation of Nuclear Envelope (NE) Reformation |
SignaLinki | Q99986 |
SIGNORi | Q99986 |
Names & Taxonomyi
Protein namesi | Recommended name: Serine/threonine-protein kinase VRK1Curated (EC:2.7.11.11 Publication)Alternative name(s): Vaccinia-related kinase 11 Publication |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:12718, VRK1 |
MIMi | 602168, gene |
neXtProti | NX_Q99986 |
VEuPathDBi | HostDB:ENSG00000100749 |
Subcellular locationi
Cytoskeleton
- spindle Source: UniProtKB-SubCell
Cytosol
- cytosol Source: HPA
Golgi apparatus
- Golgi stack Source: UniProtKB
Nucleus
- nucleolus Source: HPA
- nucleoplasm Source: HPA
- nucleus Source: UniProtKB
Other locations
- cytoplasm Source: GO_Central
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Involvement in diseasei
Pontocerebellar hypoplasia 1A (PCH1A)1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 14 | S → A: Does not abolish autophosphorylation. 1 Publication | 1 | |
Mutagenesisi | 102 | T → A: Does not abolish autophosphorylation. 1 Publication | 1 | |
Mutagenesisi | 125 | S → A: Does not abolish autophosphorylation. 1 Publication | 1 | |
Mutagenesisi | 150 | S → A: Does not abolish autophosphorylation. 1 Publication | 1 | |
Mutagenesisi | 158 | S → A: Does not abolish autophosphorylation. 1 Publication | 1 | |
Mutagenesisi | 179 | K → A: Does not affect phosphorylation at S-342. 1 Publication | 1 | |
Mutagenesisi | 239 | S → A: Does not abolish autophosphorylation. 1 Publication | 1 | |
Mutagenesisi | 305 | T → A: Does not abolish autophosphorylation. 1 Publication | 1 | |
Mutagenesisi | 312 | T → A: Does not abolish autophosphorylation. 1 Publication | 1 | |
Mutagenesisi | 342 | S → A: Abolishes phosphorylation by PLK3 and induction of Golgi fragmentation during mitosis. Strongly reduced autophosphorylation. 2 Publications | 1 | |
Mutagenesisi | 353 | T → A: Strongly reduced autophosphorylation. 1 Publication | 1 | |
Mutagenesisi | 355 | T → A: Does not abolish autophosphorylation. 1 Publication | 1 | |
Mutagenesisi | 390 | T → A: Does not abolish autophosphorylation. 1 Publication | 1 |
Keywords - Diseasei
NeurodegenerationOrganism-specific databases
DisGeNETi | 7443 |
MalaCardsi | VRK1 |
MIMi | 607596, phenotype |
OpenTargetsi | ENSG00000100749 |
Orphaneti | 423894, Microcephaly-complex motor and sensory axonal neuropathy syndrome 2254, Pontocerebellar hypoplasia type 1 |
PharmGKBi | PA37330 |
Miscellaneous databases
Pharosi | Q99986, Tbio |
Chemistry databases
ChEMBLi | CHEMBL1293199 |
Genetic variation databases
BioMutai | VRK1 |
DMDMi | 45593726 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000086803 | 1 – 396 | Serine/threonine-protein kinase VRK1Add BLAST | 396 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 71 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 342 | Phosphoserine; by PLK3Combined sources1 Publication | 1 | |
Modified residuei | 355 | Phosphothreonine; by autocatalysis1 Publication | 1 | |
Modified residuei | 376 | PhosphoserineCombined sources | 1 | |
Modified residuei | 378 | PhosphothreonineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q99986 |
jPOSTi | Q99986 |
MassIVEi | Q99986 |
MaxQBi | Q99986 |
PaxDbi | Q99986 |
PeptideAtlasi | Q99986 |
PRIDEi | Q99986 |
ProteomicsDBi | 78563 |
PTM databases
GlyGeni | Q99986, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q99986 |
PhosphoSitePlusi | Q99986 |
SwissPalmi | Q99986 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000100749, Expressed in bone marrow and 236 other tissues |
ExpressionAtlasi | Q99986, baseline and differential |
Genevisiblei | Q99986, HS |
Organism-specific databases
HPAi | ENSG00000100749, Tissue enhanced (bone marrow, testis) |
Interactioni
Subunit structurei
(Microbial infection) Interacts with vaccinia protein B12; this interaction inhibits the repressive activity of the vaccinia virus B12 pseudokinase on viral replication factory formation.
1 PublicationBinary interactionsi
Q99986
GO - Molecular functioni
- histone binding Source: GO_Central
- protein kinase binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 113282, 255 interactors |
CORUMi | Q99986 |
IntActi | Q99986, 42 interactors |
MINTi | Q99986 |
STRINGi | 9606.ENSP00000216639 |
Chemistry databases
BindingDBi | Q99986 |
Miscellaneous databases
RNActi | Q99986, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q99986 |
BMRBi | Q99986 |
SMRi | Q99986 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q99986 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 37 – 317 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 281 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 354 – 396 | DisorderedSequence analysisAdd BLAST | 43 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 356 – 371 | Basic and acidic residuesSequence analysisAdd BLAST | 16 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1164, Eukaryota |
GeneTreei | ENSGT00940000155554 |
HOGENOMi | CLU_019279_4_0_1 |
InParanoidi | Q99986 |
OMAi | PDYDKCR |
OrthoDBi | 866200at2759 |
PhylomeDBi | Q99986 |
TreeFami | TF106473 |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 37 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MPRVKAAQAG RQSSAKRHLA EQFAVGEIIT DMAKKEWKVG LPIGQGGFGC
60 70 80 90 100
IYLADMNSSE SVGSDAPCVV KVEPSDNGPL FTELKFYQRA AKPEQIQKWI
110 120 130 140 150
RTRKLKYLGV PKYWGSGLHD KNGKSYRFMI MDRFGSDLQK IYEANAKRFS
160 170 180 190 200
RKTVLQLSLR ILDILEYIHE HEYVHGDIKA SNLLLNYKNP DQVYLVDYGL
210 220 230 240 250
AYRYCPEGVH KEYKEDPKRC HDGTIEFTSI DAHNGVAPSR RGDLEILGYC
260 270 280 290 300
MIQWLTGHLP WEDNLKDPKY VRDSKIRYRE NIASLMDKCF PEKNKPGEIA
310 320 330 340 350
KYMETVKLLD YTEKPLYENL RDILLQGLKA IGSKDDGKLD LSVVENGGLK
360 370 380 390
AKTITKKRKK EIEESKEPGV EDTEWSNTQT EEAIQTRSRT RKRVQK
Computationally mapped potential isoform sequencesi
There are 37 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketH0YJF7 | H0YJF7_HUMAN | Serine/threonine-protein kinase VRK... | VRK1 hCG_24514 | 420 | Annotation score: | ||
H0YJJ9 | H0YJJ9_HUMAN | Serine/threonine-protein kinase VRK... | VRK1 | 394 | Annotation score: | ||
A0A7P0T838 | A0A7P0T838_HUMAN | Serine/threonine-protein kinase VRK... | VRK1 | 395 | Annotation score: | ||
A0A7P0T952 | A0A7P0T952_HUMAN | Serine/threonine-protein kinase VRK... | VRK1 | 393 | Annotation score: | ||
A0A7P0T9E5 | A0A7P0T9E5_HUMAN | Serine/threonine-protein kinase VRK... | VRK1 | 395 | Annotation score: | ||
A0A7P0T9T1 | A0A7P0T9T1_HUMAN | Serine/threonine-protein kinase VRK... | VRK1 | 394 | Annotation score: | ||
A0A7P0TAA6 | A0A7P0TAA6_HUMAN | Serine/threonine-protein kinase VRK... | VRK1 | 437 | Annotation score: | ||
A0A7P0TB15 | A0A7P0TB15_HUMAN | Serine/threonine-protein kinase VRK... | VRK1 | 394 | Annotation score: | ||
A0A7P0TBE4 | A0A7P0TBE4_HUMAN | Serine/threonine-protein kinase VRK... | VRK1 | 413 | Annotation score: | ||
A0A7P0Z3Z7 | A0A7P0Z3Z7_HUMAN | Serine/threonine-protein kinase VRK... | VRK1 | 395 | Annotation score: | ||
There are more potential isoformsShow all |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB000449 mRNA Translation: BAA19108.1 BC103761 mRNA Translation: AAI03762.1 BC112075 mRNA Translation: AAI12076.1 BC113510 mRNA Translation: AAI13511.1 |
CCDSi | CCDS9947.1 |
RefSeqi | NP_003375.1, NM_003384.2 |
Genome annotation databases
Ensembli | ENST00000216639.8; ENSP00000216639.3; ENSG00000100749.9 ENST00000679816.1; ENSP00000506525.1; ENSG00000100749.9 ENST00000680756.1; ENSP00000506648.1; ENSG00000100749.9 ENST00000681344.1; ENSP00000506151.1; ENSG00000100749.9 ENST00000681355.1; ENSP00000506214.1; ENSG00000100749.9 |
GeneIDi | 7443 |
KEGGi | hsa:7443 |
MANE-Selecti | ENST00000216639.8; ENSP00000216639.3; NM_003384.3; NP_003375.1 |
UCSCi | uc001yft.4, human |
Similar proteinsi
Cross-referencesi
Web resourcesi
Atlas of Genetics and Cytogenetics in Oncology and Haematology |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB000449 mRNA Translation: BAA19108.1 BC103761 mRNA Translation: AAI03762.1 BC112075 mRNA Translation: AAI12076.1 BC113510 mRNA Translation: AAI13511.1 |
CCDSi | CCDS9947.1 |
RefSeqi | NP_003375.1, NM_003384.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2KTY | NMR | - | A | 1-360 | [»] | |
2KUL | NMR | - | A | 1-360 | [»] | |
2LAV | NMR | - | A | 1-361 | [»] | |
2RSV | NMR | - | A | 1-396 | [»] | |
3OP5 | X-ray | 2.40 | A/B/C/D | 3-364 | [»] | |
5UKF | X-ray | 2.40 | A/B/C/D | 3-364 | [»] | |
5UVF | X-ray | 2.00 | A/B/C/D | 3-364 | [»] | |
6AC9 | X-ray | 2.07 | A/B/C/D | 1-364 | [»] | |
6BP0 | X-ray | 1.90 | A/B/C/D | 3-364 | [»] | |
6BRU | X-ray | 1.80 | A/B/C/D | 3-364 | [»] | |
6BTW | X-ray | 1.90 | A/B/C/D | 3-364 | [»] | |
6BU6 | X-ray | 1.80 | A/B/C/D | 3-364 | [»] | |
6CFM | X-ray | 2.45 | A/B/C/D | 3-364 | [»] | |
6CMM | X-ray | 2.10 | A/B/C/D | 3-364 | [»] | |
6CNX | X-ray | 2.00 | A/B/C/D | 3-364 | [»] | |
6CQH | X-ray | 2.15 | A/B/C/D | 3-364 | [»] | |
6CSW | X-ray | 2.25 | A/B/C/D | 3-364 | [»] | |
6DD4 | X-ray | 2.10 | A/B/C/D | 3-364 | [»] | |
6NPN | X-ray | 2.20 | A/B/C/D | 3-364 | [»] | |
6VXU | X-ray | 2.00 | A/B/C/D | 3-364 | [»] | |
6VZH | X-ray | 2.55 | A/B/C/D | 3-364 | [»] | |
7M10 | X-ray | 1.15 | B | 2-13 | [»] | |
AlphaFoldDBi | Q99986 | |||||
BMRBi | Q99986 | |||||
SMRi | Q99986 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 113282, 255 interactors |
CORUMi | Q99986 |
IntActi | Q99986, 42 interactors |
MINTi | Q99986 |
STRINGi | 9606.ENSP00000216639 |
Chemistry databases
BindingDBi | Q99986 |
ChEMBLi | CHEMBL1293199 |
PTM databases
GlyGeni | Q99986, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q99986 |
PhosphoSitePlusi | Q99986 |
SwissPalmi | Q99986 |
Genetic variation databases
BioMutai | VRK1 |
DMDMi | 45593726 |
Proteomic databases
EPDi | Q99986 |
jPOSTi | Q99986 |
MassIVEi | Q99986 |
MaxQBi | Q99986 |
PaxDbi | Q99986 |
PeptideAtlasi | Q99986 |
PRIDEi | Q99986 |
ProteomicsDBi | 78563 |
Protocols and materials databases
Antibodypediai | 42, 264 antibodies from 36 providers |
DNASUi | 7443 |
Genome annotation databases
Ensembli | ENST00000216639.8; ENSP00000216639.3; ENSG00000100749.9 ENST00000679816.1; ENSP00000506525.1; ENSG00000100749.9 ENST00000680756.1; ENSP00000506648.1; ENSG00000100749.9 ENST00000681344.1; ENSP00000506151.1; ENSG00000100749.9 ENST00000681355.1; ENSP00000506214.1; ENSG00000100749.9 |
GeneIDi | 7443 |
KEGGi | hsa:7443 |
MANE-Selecti | ENST00000216639.8; ENSP00000216639.3; NM_003384.3; NP_003375.1 |
UCSCi | uc001yft.4, human |
Organism-specific databases
CTDi | 7443 |
DisGeNETi | 7443 |
GeneCardsi | VRK1 |
HGNCi | HGNC:12718, VRK1 |
HPAi | ENSG00000100749, Tissue enhanced (bone marrow, testis) |
MalaCardsi | VRK1 |
MIMi | 602168, gene 607596, phenotype |
neXtProti | NX_Q99986 |
OpenTargetsi | ENSG00000100749 |
Orphaneti | 423894, Microcephaly-complex motor and sensory axonal neuropathy syndrome 2254, Pontocerebellar hypoplasia type 1 |
PharmGKBi | PA37330 |
VEuPathDBi | HostDB:ENSG00000100749 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1164, Eukaryota |
GeneTreei | ENSGT00940000155554 |
HOGENOMi | CLU_019279_4_0_1 |
InParanoidi | Q99986 |
OMAi | PDYDKCR |
OrthoDBi | 866200at2759 |
PhylomeDBi | Q99986 |
TreeFami | TF106473 |
Enzyme and pathway databases
PathwayCommonsi | Q99986 |
Reactomei | R-HSA-2980766, Nuclear Envelope Breakdown R-HSA-2995383, Initiation of Nuclear Envelope (NE) Reformation |
SignaLinki | Q99986 |
SIGNORi | Q99986 |
Miscellaneous databases
BioGRID-ORCSi | 7443, 442 hits in 1119 CRISPR screens |
ChiTaRSi | VRK1, human |
EvolutionaryTracei | Q99986 |
GeneWikii | VRK1 |
GenomeRNAii | 7443 |
Pharosi | Q99986, Tbio |
PROi | PR:Q99986 |
RNActi | Q99986, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000100749, Expressed in bone marrow and 236 other tissues |
ExpressionAtlasi | Q99986, baseline and differential |
Genevisiblei | Q99986, HS |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | VRK1_HUMAN | |
Accessioni | Q99986Primary (citable) accession number: Q99986 Secondary accession number(s): Q3SYL2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 10, 2003 |
Last sequence update: | May 1, 1997 | |
Last modified: | May 25, 2022 | |
This is version 201 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human and mouse protein kinases
Human and mouse protein kinases: classification and index - Human chromosome 14
Human chromosome 14: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families