UniProtKB - Q99943 (PLCA_HUMAN)
Protein
1-acyl-sn-glycerol-3-phosphate acyltransferase alpha
Gene
AGPAT1
Organism
Homo sapiens (Human)
Status
Functioni
Converts 1-acyl-sn-glycerol-3-phosphate (lysophosphatidic acid or LPA) into 1,2-diacyl-sn-glycerol-3-phosphate (phosphatidic acid or PA) by incorporating an acyl moiety at the sn-2 position of the glycerol backbone.2 Publications
Catalytic activityi
- a 1-acyl-sn-glycero-3-phosphate + an acyl-CoA = a 1,2-diacyl-sn-glycero-3-phosphate + CoA2 PublicationsEC:2.3.1.512 PublicationsThis reaction proceeds in the forward2 Publications direction.
- (9Z)-octadecenoyl-CoA + 1-(9Z-octadecenoyl)-sn-glycero-3-phosphate = 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphate + CoA2 PublicationsThis reaction proceeds in the forward2 Publications direction.
- 1-(9Z-octadecenoyl)-sn-glycero-3-phosphate + hexadecanoyl-CoA = 1-(9Z)-octadecenoyl-2-hexadecanoyl-sn-glycero-3-phosphate + CoA2 PublicationsThis reaction proceeds in the forward2 Publications direction.
- 1-(9Z-octadecenoyl)-sn-glycero-3-phosphate + heptadecanoyl-CoA = 1-(9Z)-octadecenoyl-2-heptadecanoyl-sn-glycero-3-phosphate + CoA1 PublicationThis reaction proceeds in the forward1 Publication direction.
- 1-(9Z-octadecenoyl)-sn-glycero-3-phosphate + octadecanoyl-CoA = 1-(9Z-octadecenoyl)-2-octadecanoyl-sn-glycero-3-phosphate + CoA2 PublicationsThis reaction proceeds in the forward2 Publications direction.
- (9Z,12Z)-octadecadienoyl-CoA + 1-(9Z-octadecenoyl)-sn-glycero-3-phosphate = 1-(9Z)-octadecenoyl-2-(9Z,12Z)-octadecadienoyl-sn-glycero-3-phosphate + CoA2 PublicationsThis reaction proceeds in the forward2 Publications direction.
- 1-(9Z-octadecenoyl)-sn-glycero-3-phosphate + tetradecanoyl-CoA = 1-(9Z)-octadecenoyl-2-tetradecanoyl-sn-glycero-3-phosphate + CoA2 PublicationsThis reaction proceeds in the forward2 Publications direction.
- 1-(9Z-octadecenoyl)-sn-glycero-3-phosphate + pentadecanoyl-CoA = 1-(9Z)-octadecenoyl-2-pentadecanoyl-sn-glycero-3-phosphate + CoA1 PublicationThis reaction proceeds in the forward1 Publication direction.
- (9Z)-octadecenoyl-CoA + 1-hexadecanoyl-sn-glycero-3-phosphate = 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphate + CoA1 PublicationThis reaction proceeds in the forward1 Publication direction.
- (9Z)-octadecenoyl-CoA + 1-(9Z,12Z,15Z)-octadecatrienoyl-sn-glycero-3-phosphate = 1-(9Z,12Z,15Z)-octadecatrienoyl-2-(9Z)-octadecenoyl-sn-glycero-3-phosphate + CoA1 PublicationThis reaction proceeds in the forward1 Publication direction.
- (9Z)-octadecenoyl-CoA + 1-(6Z,9Z,12Z-octadecatrienoyl)-sn-glycero-3-phosphate = (6Z,9Z,12Z)-octadecatrienoyl-2-(9Z)-octadecenoyl-sn-glycero-3-phosphate + CoA1 PublicationThis reaction proceeds in the forward1 Publication direction.
- (9Z)-octadecenoyl-CoA + 1-eicosanoyl-sn-glycero-3-phosphate = 1-eicosanoyl-2-(9Z)-octadecenoyl-sn-glycero-3-phosphate + CoA1 PublicationThis reaction proceeds in the forward1 Publication direction.
- (9Z)-octadecenoyl-CoA + 1-tetradecanoyl-sn-glycerol 3-phosphate = 1-tetradecanoyl-2-(9Z)-octadecenoyl-sn-glycero-3-phosphate + CoA1 PublicationThis reaction proceeds in the forward1 Publication direction.
- (5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA + 1-(9Z-octadecenoyl)-sn-glycero-3-phosphate = 1-(9Z)-octadecenoyl-2-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-sn-glycero-3-phosphate + CoA1 PublicationThis reaction proceeds in the forward1 Publication direction.
- 1-(9Z-octadecenoyl)-sn-glycero-3-phosphate + dodecanoyl-CoA = 1-(9Z)-octadecenoyl-2-dodecanoyl-sn-glycero-3-phosphate + CoA1 PublicationThis reaction proceeds in the forward1 Publication direction.
- (6Z)-octadecenoyl-CoA + 1-(9Z-octadecenoyl)-sn-glycero-3-phosphate = 1-(9Z)-octadecenoyl-2-(6Z)-octadecenoyl-sn-glycero-3-phosphate + CoA1 PublicationThis reaction proceeds in the forward1 Publication direction.
- (11Z)-octadecenoyl-CoA + 1-(9Z-octadecenoyl)-sn-glycero-3-phosphate = 1-(9Z)-octadecenoyl-2-(11Z)-octadecenoyl-sn-glycero-3-phosphate + CoA1 PublicationThis reaction proceeds in the forward1 Publication direction.
- (9Z)-hexadecenoyl-CoA + 1-(9Z-octadecenoyl)-sn-glycero-3-phosphate = 1-(9Z-octadecenoyl)-2-(9Z-hexadecenoyl)-sn-glycero-3-phosphate + CoA1 PublicationThis reaction proceeds in the forward1 Publication direction.
Kineticsi
- KM=137.97 µM for C14:0-CoA1 Publication
- KM=3.04 µM for C15:0-CoA1 Publication
- KM=116.73 µM for C18:0-CoA1 Publication
- KM=39.37 µM for C18:1-CoA1 Publication
- KM=6.0 µM for LPA sn-1 C18:11 Publication
- Vmax=96.21 nmol/min/mg enzyme for C14:0-CoA1 Publication
- Vmax=86.09 nmol/min/mg enzyme for C15:0-CoA1 Publication
- Vmax=91.94 nmol/min/mg enzyme for C18:0-CoA1 Publication
- Vmax=77.57 nmol/min/mg enzyme for C18:1-CoA1 Publication
- Vmax=92.0 nmol/min/mg enzyme for LPA sn-1 C18:11 Publication
: CDP-diacylglycerol biosynthesis Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate.Proteins known to be involved in the 3 steps of the subpathway in this organism are:
- Glycerol-3-phosphate acyltransferase 1, mitochondrial, Glycerol-3-phosphate acyltransferase 2, mitochondrial (GPAT2), Glycerol-3-phosphate acyltransferase 1, mitochondrial (GPAM), Glycerol-3-phosphate acyltransferase 1, mitochondrial (GPAM), Glycerol-3-phosphate acyltransferase 1, mitochondrial (DKFZp451B1115), Glycerol-3-phosphate acyltransferase 1, mitochondrial (GPAM), Glycerol-3-phosphate acyltransferase 4 (GPAT4), Glycerol-3-phosphate acyltransferase 3 (GPAT3)
- 1-acyl-sn-glycerol-3-phosphate acyltransferase epsilon (AGPAT5), 1-acyl-sn-glycerol-3-phosphate acyltransferase gamma (AGPAT3), 1-acyl-sn-glycerol-3-phosphate acyltransferase alpha (AGPAT1), 1-acyl-sn-glycerol-3-phosphate acyltransferase beta (AGPAT2), Lysocardiolipin acyltransferase 1 (LCLAT1), 1-acyl-sn-glycerol-3-phosphate acyltransferase delta (AGPAT4)
- Phosphatidate cytidylyltransferase 1 (CDS1), Phosphatidate cytidylyltransferase 2 (CDS2), Phosphatidate cytidylyltransferase, mitochondrial (TAMM41), CDP-diacylglycerol synthase (TAMM41), Phosphatidate cytidylyltransferase (CDS1), CDP-diacylglycerol synthase (TAMM41), Phosphatidate cytidylyltransferase, Phosphatidate cytidylyltransferase, Phosphatidate cytidylyltransferase, CDP-diacylglycerol synthase (TAMM41), CDP-diacylglycerol synthase (TAMM41), Phosphatidate cytidylyltransferase
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate, the pathway CDP-diacylglycerol biosynthesis and in Phospholipid metabolism.
GO - Molecular functioni
- 1-acylglycerol-3-phosphate O-acyltransferase activity Source: UniProtKB
GO - Biological processi
- CDP-diacylglycerol biosynthetic process Source: UniProtKB-UniPathway
- phosphatidic acid biosynthetic process Source: BHF-UCL
- phospholipid metabolic process Source: ProtInc
- positive regulation of cellular metabolic process Source: Reactome
- positive regulation of cytokine-mediated signaling pathway Source: BHF-UCL
- positive regulation of cytokine production Source: BHF-UCL
Keywordsi
Molecular function | Acyltransferase, Transferase |
Biological process | Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism |
Enzyme and pathway databases
BioCyci | MetaCyc:HS09762-MONOMER |
BRENDAi | 2.3.1.51, 2681 |
PathwayCommonsi | Q99943 |
Reactomei | R-HSA-1483166, Synthesis of PA R-HSA-163765, ChREBP activates metabolic gene expression |
UniPathwayi | UPA00557;UER00613 |
Chemistry databases
SwissLipidsi | SLP:000000095 |
Names & Taxonomyi
Protein namesi | Recommended name: 1-acyl-sn-glycerol-3-phosphate acyltransferase alpha (EC:2.3.1.512 Publications)Alternative name(s): 1-acylglycerol-3-phosphate O-acyltransferase 1 Short name: 1-AGP acyltransferase 1 Short name: 1-AGPAT 1 Lysophosphatidic acid acyltransferase alpha1 Publication Short name: LPAAT-alpha Protein G15 |
Gene namesi | Name:AGPAT1 Synonyms:G15 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:324, AGPAT1 |
MIMi | 603099, gene |
neXtProti | NX_Q99943 |
VEuPathDBi | HostDB:ENSG00000204310.10 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane 2 Publications; Multi-pass membrane protein Sequence analysis
Endoplasmic reticulum
- endoplasmic reticulum Source: UniProtKB
- endoplasmic reticulum membrane Source: Reactome
Other locations
- integral component of membrane Source: UniProtKB-KW
- membrane Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 27 – 37 | Lumenal1 PublicationAdd BLAST | 11 | |
Transmembranei | 38 – 58 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 59 – 127 | Cytoplasmic1 PublicationAdd BLAST | 69 | |
Transmembranei | 128 – 148 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 149 – 283 | Lumenal1 PublicationAdd BLAST | 135 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 10554 |
OpenTargetsi | ENSG00000204310 |
PharmGKBi | PA24621 |
Miscellaneous databases
Pharosi | Q99943, Tbio |
Chemistry databases
ChEMBLi | CHEMBL3583 |
Genetic variation databases
BioMutai | AGPAT1 |
DMDMi | 3914372 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 26 | Sequence analysisAdd BLAST | 26 | |
ChainiPRO_0000208190 | 27 – 283 | 1-acyl-sn-glycerol-3-phosphate acyltransferase alphaAdd BLAST | 257 |
Proteomic databases
EPDi | Q99943 |
jPOSTi | Q99943 |
MassIVEi | Q99943 |
MaxQBi | Q99943 |
PaxDbi | Q99943 |
PeptideAtlasi | Q99943 |
PRIDEi | Q99943 |
ProteomicsDBi | 78534 |
PTM databases
iPTMneti | Q99943 |
PhosphoSitePlusi | Q99943 |
SwissPalmi | Q99943 |
Expressioni
Tissue specificityi
Widely expressed. Expressed in adipose tissue and at high levels in testis and pancreas. Expressed at lower levels in tissues such as heart, brain, placenta, kidney, lung, spleen, thymus, prostate, ovary, intestine, colon, leukocyte and liver.1 Publication
Gene expression databases
Bgeei | ENSG00000204310, Expressed in right frontal lobe and 234 other tissues |
ExpressionAtlasi | Q99943, baseline and differential |
Genevisiblei | Q99943, HS |
Organism-specific databases
HPAi | ENSG00000204310, Low tissue specificity |
Interactioni
Binary interactionsi
Q99943
With | #Exp. | IntAct |
---|---|---|
MEOX2 [P50222] | 3 | EBI-3893468,EBI-748397 |
Protein-protein interaction databases
BioGRIDi | 115805, 34 interactors |
IntActi | Q99943, 10 interactors |
STRINGi | 9606.ENSP00000378877 |
Miscellaneous databases
RNActi | Q99943, protein |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 104 – 109 | HXXXXD motifBy similarity | 6 | |
Motifi | 178 – 181 | EGTR motif1 Publication | 4 |
Domaini
The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.By similarity
Sequence similaritiesi
Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.Curated
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG2848, Eukaryota |
GeneTreei | ENSGT00390000008726 |
HOGENOMi | CLU_027938_10_1_1 |
InParanoidi | Q99943 |
OMAi | FHMAIDQ |
OrthoDBi | 1623097at2759 |
PhylomeDBi | Q99943 |
TreeFami | TF314867 |
Family and domain databases
InterProi | View protein in InterPro IPR004552, AGP_acyltrans IPR002123, Plipid/glycerol_acylTrfase |
Pfami | View protein in Pfam PF01553, Acyltransferase, 1 hit |
SMARTi | View protein in SMART SM00563, PlsC, 1 hit |
TIGRFAMsi | TIGR00530, AGP_acyltrn, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q99943-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDLWPGAWML LLLLFLLLLF LLPTLWFCSP SAKYFFKMAF YNGWILFLAV
60 70 80 90 100
LAIPVCAVRG RNVENMKILR LMLLHIKYLY GIRVEVRGAH HFPPSQPYVV
110 120 130 140 150
VSNHQSSLDL LGMMEVLPGR CVPIAKRELL WAGSAGLACW LAGVIFIDRK
160 170 180 190 200
RTGDAISVMS EVAQTLLTQD VRVWVFPEGT RNHNGSMLPF KRGAFHLAVQ
210 220 230 240 250
AQVPIVPIVM SSYQDFYCKK ERRFTSGQCQ VRVLPPVPTE GLTPDDVPAL
260 270 280
ADRVRHSMLT VFREISTDGR GGGDYLKKPG GGG
Sequence cautioni
The sequence AAB47493 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_050593 | 30 | P → S. Corresponds to variant dbSNP:rs11964847Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U56417 mRNA Translation: AAB58775.1 U75971 mRNA Translation: AAB96378.1 Y09565 mRNA Translation: CAA70758.1 U89336 Genomic DNA Translation: AAB47493.1 Different initiation. AL662884 Genomic DNA No translation available. AL662828 Genomic DNA No translation available. AL845464 Genomic DNA No translation available. BX284686 Genomic DNA No translation available. BX927239 Genomic DNA No translation available. CR933878 Genomic DNA No translation available. CR812478 Genomic DNA No translation available. CH471081 Genomic DNA Translation: EAX03600.1 BC002402 mRNA Translation: AAH02402.1 BC003007 mRNA Translation: AAH03007.1 BC004310 mRNA Translation: AAH04310.1 BC090849 mRNA Translation: AAH90849.1 |
CCDSi | CCDS4744.1 |
RefSeqi | NP_006402.1, NM_006411.3 NP_116130.2, NM_032741.4 XP_005248863.1, XM_005248806.2 XP_011512536.1, XM_011514234.1 |
Genome annotation databases
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U56417 mRNA Translation: AAB58775.1 U75971 mRNA Translation: AAB96378.1 Y09565 mRNA Translation: CAA70758.1 U89336 Genomic DNA Translation: AAB47493.1 Different initiation. AL662884 Genomic DNA No translation available. AL662828 Genomic DNA No translation available. AL845464 Genomic DNA No translation available. BX284686 Genomic DNA No translation available. BX927239 Genomic DNA No translation available. CR933878 Genomic DNA No translation available. CR812478 Genomic DNA No translation available. CH471081 Genomic DNA Translation: EAX03600.1 BC002402 mRNA Translation: AAH02402.1 BC003007 mRNA Translation: AAH03007.1 BC004310 mRNA Translation: AAH04310.1 BC090849 mRNA Translation: AAH90849.1 |
CCDSi | CCDS4744.1 |
RefSeqi | NP_006402.1, NM_006411.3 NP_116130.2, NM_032741.4 XP_005248863.1, XM_005248806.2 XP_011512536.1, XM_011514234.1 |
3D structure databases
SMRi | Q99943 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 115805, 34 interactors |
IntActi | Q99943, 10 interactors |
STRINGi | 9606.ENSP00000378877 |
Chemistry databases
ChEMBLi | CHEMBL3583 |
SwissLipidsi | SLP:000000095 |
PTM databases
iPTMneti | Q99943 |
PhosphoSitePlusi | Q99943 |
SwissPalmi | Q99943 |
Genetic variation databases
BioMutai | AGPAT1 |
DMDMi | 3914372 |
Proteomic databases
EPDi | Q99943 |
jPOSTi | Q99943 |
MassIVEi | Q99943 |
MaxQBi | Q99943 |
PaxDbi | Q99943 |
PeptideAtlasi | Q99943 |
PRIDEi | Q99943 |
ProteomicsDBi | 78534 |
Protocols and materials databases
Antibodypediai | 28453, 130 antibodies |
Genome annotation databases
Organism-specific databases
CTDi | 10554 |
DisGeNETi | 10554 |
GeneCardsi | AGPAT1 |
HGNCi | HGNC:324, AGPAT1 |
HPAi | ENSG00000204310, Low tissue specificity |
MIMi | 603099, gene |
neXtProti | NX_Q99943 |
OpenTargetsi | ENSG00000204310 |
PharmGKBi | PA24621 |
VEuPathDBi | HostDB:ENSG00000204310.10 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2848, Eukaryota |
GeneTreei | ENSGT00390000008726 |
HOGENOMi | CLU_027938_10_1_1 |
InParanoidi | Q99943 |
OMAi | FHMAIDQ |
OrthoDBi | 1623097at2759 |
PhylomeDBi | Q99943 |
TreeFami | TF314867 |
Enzyme and pathway databases
UniPathwayi | UPA00557;UER00613 |
BioCyci | MetaCyc:HS09762-MONOMER |
BRENDAi | 2.3.1.51, 2681 |
PathwayCommonsi | Q99943 |
Reactomei | R-HSA-1483166, Synthesis of PA R-HSA-163765, ChREBP activates metabolic gene expression |
Miscellaneous databases
BioGRID-ORCSi | 10554, 27 hits in 873 CRISPR screens |
ChiTaRSi | AGPAT1, human |
GeneWikii | AGPAT1 |
GenomeRNAii | 10554 |
Pharosi | Q99943, Tbio |
PROi | PR:Q99943 |
RNActi | Q99943, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000204310, Expressed in right frontal lobe and 234 other tissues |
ExpressionAtlasi | Q99943, baseline and differential |
Genevisiblei | Q99943, HS |
Family and domain databases
InterProi | View protein in InterPro IPR004552, AGP_acyltrans IPR002123, Plipid/glycerol_acylTrfase |
Pfami | View protein in Pfam PF01553, Acyltransferase, 1 hit |
SMARTi | View protein in SMART SM00563, PlsC, 1 hit |
TIGRFAMsi | TIGR00530, AGP_acyltrn, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PLCA_HUMAN | |
Accessioni | Q99943Primary (citable) accession number: Q99943 Secondary accession number(s): A2BFI5, Q5BL03 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 15, 1998 |
Last sequence update: | January 1, 1998 | |
Last modified: | February 10, 2021 | |
This is version 181 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 6
Human chromosome 6: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families