UniProtKB - Q99942 (RNF5_HUMAN)
Protein
E3 ubiquitin-protein ligase RNF5
Gene
RNF5
Organism
Homo sapiens (Human)
Status
Functioni
Has E2-dependent E3 ubiquitin-protein ligase activity. May function together with E2 ubiquitin-conjugating enzymes UBE2D1/UBCH5A and UBE2D2/UBC4. Mediates ubiquitination of PXN/paxillin and Salmonella type III secreted protein sopA. May be involved in regulation of cell motility and localization of PXN/paxillin. Mediates the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD; the ubiquitination appears to involve E2 ubiquitin-conjugating enzyme UBE2N. Mediates the 'Lys-48'-linked polyubiquitination of STING1 at 'Lys-150' leading to its proteasomal degradation; the ubiquitination occurs in mitochondria after viral transfection and regulates antiviral responses.5 Publications
Catalytic activityi
- S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine. EC:2.3.2.27
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 27 – 68 | RING-typePROSITE-ProRule annotationAdd BLAST | 42 |
GO - Molecular functioni
- identical protein binding Source: IntAct
- protein-containing complex binding Source: ParkinsonsUK-UCL
- ubiquitin-like protein conjugating enzyme binding Source: GO_Central
- ubiquitin protein ligase activity Source: GO_Central
- ubiquitin-protein transferase activity Source: UniProtKB
- zinc ion binding Source: ProtInc
GO - Biological processi
- cellular protein catabolic process Source: UniProtKB
- endoplasmic reticulum mannose trimming Source: Reactome
- ERAD pathway Source: ParkinsonsUK-UCL
- ER-associated misfolded protein catabolic process Source: UniProtKB
- negative regulation of autophagy Source: Ensembl
- protein destabilization Source: Ensembl
- protein K48-linked ubiquitination Source: UniProtKB
- protein K63-linked ubiquitination Source: UniProtKB
- regulation of autophagosome assembly Source: Ensembl
- response to bacterium Source: Ensembl
- transmembrane transport Source: Reactome
- ubiquitin-dependent ERAD pathway Source: ParkinsonsUK-UCL
- ubiquitin-dependent protein catabolic process Source: GO_Central
Keywordsi
Molecular function | Transferase |
Biological process | Ubl conjugation pathway |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | Q99942 |
Reactomei | R-HSA-382556, ABC-family proteins mediated transport R-HSA-5678895, Defective CFTR causes cystic fibrosis R-HSA-901032, ER Quality Control Compartment (ERQC) |
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: E3 ubiquitin-protein ligase RNF5 (EC:2.3.2.27)Alternative name(s): Protein G16 RING finger protein 5 RING-type E3 ubiquitin transferase RNF5Curated Ram1 homolog Short name: HsRma1 |
Gene namesi | Name:RNF5 Synonyms:G16, NG2, RMA1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000204308.7 |
HGNCi | HGNC:10068, RNF5 |
MIMi | 602677, gene |
neXtProti | NX_Q99942 |
Subcellular locationi
Endoplasmic reticulum
Mitochondrion
Other locations
Note: Predominantly located in the plasma membrane, with some localization occurring within cytoplasmic organelles.
Endoplasmic reticulum
- endoplasmic reticulum Source: HPA
- endoplasmic reticulum membrane Source: Reactome
- endoplasmic reticulum quality control compartment Source: GOC
Mitochondrion
- mitochondrial membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 118 – 138 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 160 – 180 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Endoplasmic reticulum, Membrane, MitochondrionPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 42 | C → S: Loss of E3 ubiquitin-protein ligase activity. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 6048 |
OpenTargetsi | ENSG00000204308 |
PharmGKBi | PA34442 |
Miscellaneous databases
Pharosi | Q99942, Tbio |
Polymorphism and mutation databases
BioMutai | RNF5 |
DMDMi | 74762702 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources1 Publication | |||
ChainiPRO_0000240393 | 2 – 180 | E3 ubiquitin-protein ligase RNF5Add BLAST | 179 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources1 Publication | 1 | |
Modified residuei | 84 | PhosphoserineCombined sources | 1 | |
Modified residuei | 94 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 107 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q99942 |
jPOSTi | Q99942 |
MassIVEi | Q99942 |
MaxQBi | Q99942 |
PaxDbi | Q99942 |
PeptideAtlasi | Q99942 |
PRIDEi | Q99942 |
ProteomicsDBi | 78533 |
TopDownProteomicsi | Q99942 |
PTM databases
iPTMneti | Q99942 |
PhosphoSitePlusi | Q99942 |
Expressioni
Tissue specificityi
Widely expressed.1 Publication
Gene expression databases
Bgeei | ENSG00000204308, Expressed in heart left ventricle and 121 other tissues |
ExpressionAtlasi | Q99942, baseline and differential |
Genevisiblei | Q99942, HS |
Organism-specific databases
HPAi | ENSG00000204308, Low tissue specificity |
Interactioni
Subunit structurei
Interacts with PXN.
Interacts with Salmonella typhimurium sopA.
Interacts with JKAMP.
Interacts with STING1; the interaction of endogenous proteins is dependent on viral infection.
3 PublicationsBinary interactionsi
Hide detailsQ99942
GO - Molecular functioni
- identical protein binding Source: IntAct
- ubiquitin-like protein conjugating enzyme binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 111975, 81 interactors |
DIPi | DIP-29268N |
IntActi | Q99942, 68 interactors |
MINTi | Q99942 |
STRINGi | 9606.ENSP00000364235 |
Miscellaneous databases
RNActi | Q99942, protein |
Family & Domainsi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 27 – 68 | RING-typePROSITE-ProRule annotationAdd BLAST | 42 |
Keywords - Domaini
Transmembrane, Transmembrane helix, Zinc-fingerPhylogenomic databases
eggNOGi | KOG0823, Eukaryota |
GeneTreei | ENSGT00390000014107 |
HOGENOMi | CLU_055198_2_1_1 |
InParanoidi | Q99942 |
OMAi | WPCLHQV |
OrthoDBi | 1510545at2759 |
PhylomeDBi | Q99942 |
TreeFami | TF317334 |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD IPR017907, Znf_RING_CS |
SMARTi | View protein in SMART SM00184, RING, 1 hit |
PROSITEi | View protein in PROSITE PS00518, ZF_RING_1, 1 hit PS50089, ZF_RING_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q99942-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAAAEEEDGG PEGPNRERGG AGATFECNIC LETAREAVVS VCGHLYCWPC
60 70 80 90 100
LHQWLETRPE RQECPVCKAG ISREKVVPLY GRGSQKPQDP RLKTPPRPQG
110 120 130 140 150
QRPAPESRGG FQPFGDTGGF HFSFGVGAFP FGFFTTVFNA HEPFRRGTGV
160 170 180
DLGQGHPASS WQDSLFLFLA IFFFFWLLSI
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A140TA09 | A0A140TA09_HUMAN | E3 ubiquitin-protein ligase RNF5 | RNF5 | 91 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 22 | G → S in CAB51286 (Ref. 3) Curated | 1 | |
Sequence conflicti | 60 | E → D in CAB51286 (Ref. 3) Curated | 1 | |
Sequence conflicti | 117 | T → A in CAB51286 (Ref. 3) Curated | 1 | |
Sequence conflicti | 148 | T → A in CAB51286 (Ref. 3) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB056869 mRNA Translation: BAB39359.1 AJ243936 mRNA Translation: CAB51286.1 AK311859 mRNA Translation: BAG34800.1 BT007105 mRNA Translation: AAP35769.1 U89336 Genomic DNA Translation: AAB47492.1 AL845464 Genomic DNA No translation available. AL662884 Genomic DNA No translation available. AL662830 Genomic DNA No translation available. BX284686 Genomic DNA No translation available. BX927239 Genomic DNA No translation available. CR812478 Genomic DNA No translation available. CR933878 Genomic DNA No translation available. BC004155 mRNA Translation: AAH04155.1 BC111392 mRNA Translation: AAI11393.1 BC119741 mRNA Translation: AAI19742.1 BC119742 mRNA Translation: AAI19743.1 BC127651 mRNA Translation: AAI27652.1 BC127652 mRNA Translation: AAI27653.1 BC148255 mRNA Translation: AAI48256.1 |
CCDSi | CCDS4745.1 |
RefSeqi | NP_008844.1, NM_006913.3 |
Genome annotation databases
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB056869 mRNA Translation: BAB39359.1 AJ243936 mRNA Translation: CAB51286.1 AK311859 mRNA Translation: BAG34800.1 BT007105 mRNA Translation: AAP35769.1 U89336 Genomic DNA Translation: AAB47492.1 AL845464 Genomic DNA No translation available. AL662884 Genomic DNA No translation available. AL662830 Genomic DNA No translation available. BX284686 Genomic DNA No translation available. BX927239 Genomic DNA No translation available. CR812478 Genomic DNA No translation available. CR933878 Genomic DNA No translation available. BC004155 mRNA Translation: AAH04155.1 BC111392 mRNA Translation: AAI11393.1 BC119741 mRNA Translation: AAI19742.1 BC119742 mRNA Translation: AAI19743.1 BC127651 mRNA Translation: AAI27652.1 BC127652 mRNA Translation: AAI27653.1 BC148255 mRNA Translation: AAI48256.1 |
CCDSi | CCDS4745.1 |
RefSeqi | NP_008844.1, NM_006913.3 |
3D structure databases
SMRi | Q99942 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 111975, 81 interactors |
DIPi | DIP-29268N |
IntActi | Q99942, 68 interactors |
MINTi | Q99942 |
STRINGi | 9606.ENSP00000364235 |
PTM databases
iPTMneti | Q99942 |
PhosphoSitePlusi | Q99942 |
Polymorphism and mutation databases
BioMutai | RNF5 |
DMDMi | 74762702 |
Proteomic databases
EPDi | Q99942 |
jPOSTi | Q99942 |
MassIVEi | Q99942 |
MaxQBi | Q99942 |
PaxDbi | Q99942 |
PeptideAtlasi | Q99942 |
PRIDEi | Q99942 |
ProteomicsDBi | 78533 |
TopDownProteomicsi | Q99942 |
Protocols and materials databases
Antibodypediai | 28471, 222 antibodies |
DNASUi | 6048 |
Genome annotation databases
Organism-specific databases
CTDi | 6048 |
DisGeNETi | 6048 |
EuPathDBi | HostDB:ENSG00000204308.7 |
GeneCardsi | RNF5 |
HGNCi | HGNC:10068, RNF5 |
HPAi | ENSG00000204308, Low tissue specificity |
MIMi | 602677, gene |
neXtProti | NX_Q99942 |
OpenTargetsi | ENSG00000204308 |
PharmGKBi | PA34442 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0823, Eukaryota |
GeneTreei | ENSGT00390000014107 |
HOGENOMi | CLU_055198_2_1_1 |
InParanoidi | Q99942 |
OMAi | WPCLHQV |
OrthoDBi | 1510545at2759 |
PhylomeDBi | Q99942 |
TreeFami | TF317334 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
PathwayCommonsi | Q99942 |
Reactomei | R-HSA-382556, ABC-family proteins mediated transport R-HSA-5678895, Defective CFTR causes cystic fibrosis R-HSA-901032, ER Quality Control Compartment (ERQC) |
Miscellaneous databases
BioGRID-ORCSi | 6048, 89 hits in 845 CRISPR screens |
GeneWikii | RNF5 |
GenomeRNAii | 6048 |
Pharosi | Q99942, Tbio |
PROi | PR:Q99942 |
RNActi | Q99942, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000204308, Expressed in heart left ventricle and 121 other tissues |
ExpressionAtlasi | Q99942, baseline and differential |
Genevisiblei | Q99942, HS |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD IPR017907, Znf_RING_CS |
SMARTi | View protein in SMART SM00184, RING, 1 hit |
PROSITEi | View protein in PROSITE PS00518, ZF_RING_1, 1 hit PS50089, ZF_RING_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RNF5_HUMAN | |
Accessioni | Q99942Primary (citable) accession number: Q99942 Secondary accession number(s): A2BFI6 Q9UMQ2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 27, 2006 |
Last sequence update: | May 1, 1997 | |
Last modified: | December 2, 2020 | |
This is version 188 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 6
Human chromosome 6: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways