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Protein

E3 ubiquitin-protein ligase RNF5

Gene

RNF5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Has E2-dependent E3 ubiquitin-protein ligase activity. May function together with E2 ubiquitin-conjugating enzymes UBE2D1/UBCH5A and UBE2D2/UBC4. Mediates ubiquitination of PXN/paxillin and Salmonella type III secreted protein sopA. May be involved in regulation of cell motility and localization of PXN/paxillin. Mediates the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD; the ubiquitination appears to involve E2 ubiquitin-conjugating enzyme UBE2N. Mediates the 'Lys-48'-linked polyubiquitination of TMEM173 at 'Lys-150' leading to its proteasomal degradation; the ubiquitination occurs in mitochondria after viral transfection and regulates antiviral responses.5 Publications

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri27 – 68RING-typePROSITE-ProRule annotationAdd BLAST42

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • ubiquitin-like protein conjugating enzyme binding Source: GO_Central
  • ubiquitin protein ligase activity Source: ParkinsonsUK-UCL
  • ubiquitin-protein transferase activity Source: UniProtKB
  • zinc ion binding Source: ProtInc

GO - Biological processi

  • cellular protein catabolic process Source: UniProtKB
  • endoplasmic reticulum mannose trimming Source: Reactome
  • ERAD pathway Source: ParkinsonsUK-UCL
  • ER-associated misfolded protein catabolic process Source: UniProtKB
  • negative regulation of autophagy Source: Ensembl
  • protein destabilization Source: Ensembl
  • protein K48-linked ubiquitination Source: UniProtKB
  • protein K63-linked ubiquitination Source: UniProtKB
  • regulation of autophagosome assembly Source: Ensembl
  • response to bacterium Source: Ensembl
  • transmembrane transport Source: Reactome
  • ubiquitin-dependent ERAD pathway Source: ParkinsonsUK-UCL

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-382556 ABC-family proteins mediated transport
R-HSA-5678895 Defective CFTR causes cystic fibrosis
R-HSA-901032 ER Quality Control Compartment (ERQC)
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase RNF5 (EC:2.3.2.27)
Alternative name(s):
Protein G16
RING finger protein 5
RING-type E3 ubiquitin transferase RNF5Curated
Ram1 homolog
Short name:
HsRma1
Gene namesi
Name:RNF5
Synonyms:G16, NG2, RMA1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

EuPathDBiHostDB:ENSG00000204308.7
HGNCiHGNC:10068 RNF5
MIMi602677 gene
neXtProtiNX_Q99942

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei118 – 138HelicalSequence analysisAdd BLAST21
Transmembranei160 – 180HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Mitochondrion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi42C → S: Loss of E3 ubiquitin-protein ligase activity. 1 Publication1

Organism-specific databases

DisGeNETi6048
OpenTargetsiENSG00000204308
PharmGKBiPA34442

Polymorphism and mutation databases

BioMutaiRNF5
DMDMi74762702

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources1 Publication
ChainiPRO_00002403932 – 180E3 ubiquitin-protein ligase RNF5Add BLAST179

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1 Publication1
Modified residuei84PhosphoserineCombined sources1
Modified residuei94PhosphothreonineCombined sources1
Modified residuei107PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ99942
MaxQBiQ99942
PaxDbiQ99942
PeptideAtlasiQ99942
PRIDEiQ99942
ProteomicsDBi78533
TopDownProteomicsiQ99942

PTM databases

iPTMnetiQ99942
PhosphoSitePlusiQ99942

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Gene expression databases

BgeeiENSG00000204308
CleanExiHS_RNF5
ExpressionAtlasiQ99942 baseline and differential
GenevisibleiQ99942 HS

Organism-specific databases

HPAiCAB034107
HPA065032

Interactioni

Subunit structurei

Interacts with PXN. Interacts with Salmonella typhimurium sopA. Interacts with JKAMP. Interacts with TMEM173; the interaction of endogenous proteins is dependent on viral infection.3 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • ubiquitin-like protein conjugating enzyme binding Source: GO_Central

Protein-protein interaction databases

BioGridi111975, 60 interactors
DIPiDIP-29268N
IntActiQ99942, 64 interactors
MINTiQ99942
STRINGi9606.ENSP00000364235

Structurei

3D structure databases

ProteinModelPortaliQ99942
SMRiQ99942
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri27 – 68RING-typePROSITE-ProRule annotationAdd BLAST42

Keywords - Domaini

Transmembrane, Transmembrane helix, Zinc-finger

Phylogenomic databases

eggNOGiKOG0823 Eukaryota
ENOG4111IHV LUCA
GeneTreeiENSGT00390000014107
HOGENOMiHOG000238304
HOVERGENiHBG054495
InParanoidiQ99942
KOiK10666
OMAiTVFNTND
OrthoDBiEOG091G0W2I
PhylomeDBiQ99942
TreeFamiTF317334

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR017907 Znf_RING_CS
SMARTiView protein in SMART
SM00184 RING, 1 hit
PROSITEiView protein in PROSITE
PS00518 ZF_RING_1, 1 hit
PS50089 ZF_RING_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99942-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAAEEEDGG PEGPNRERGG AGATFECNIC LETAREAVVS VCGHLYCWPC
60 70 80 90 100
LHQWLETRPE RQECPVCKAG ISREKVVPLY GRGSQKPQDP RLKTPPRPQG
110 120 130 140 150
QRPAPESRGG FQPFGDTGGF HFSFGVGAFP FGFFTTVFNA HEPFRRGTGV
160 170 180
DLGQGHPASS WQDSLFLFLA IFFFFWLLSI
Length:180
Mass (Da):19,881
Last modified:May 1, 1997 - v1
Checksum:iE5AFA4DE6DE85942
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti22G → S in CAB51286 (Ref. 3) Curated1
Sequence conflicti60E → D in CAB51286 (Ref. 3) Curated1
Sequence conflicti117T → A in CAB51286 (Ref. 3) Curated1
Sequence conflicti148T → A in CAB51286 (Ref. 3) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB056869 mRNA Translation: BAB39359.1
AJ243936 mRNA Translation: CAB51286.1
AK311859 mRNA Translation: BAG34800.1
BT007105 mRNA Translation: AAP35769.1
U89336 Genomic DNA Translation: AAB47492.1
AL845464 Genomic DNA No translation available.
AL662884 Genomic DNA No translation available.
AL662830 Genomic DNA No translation available.
BX284686 Genomic DNA No translation available.
BX927239 Genomic DNA No translation available.
CR812478 Genomic DNA No translation available.
CR933878 Genomic DNA No translation available.
BC004155 mRNA Translation: AAH04155.1
BC111392 mRNA Translation: AAI11393.1
BC119741 mRNA Translation: AAI19742.1
BC119742 mRNA Translation: AAI19743.1
BC127651 mRNA Translation: AAI27652.1
BC127652 mRNA Translation: AAI27653.1
BC148255 mRNA Translation: AAI48256.1
CCDSiCCDS4745.1
RefSeqiNP_008844.1, NM_006913.3
UniGeneiHs.731774

Genome annotation databases

EnsembliENST00000375094; ENSP00000364235; ENSG00000204308
ENST00000413786; ENSP00000387879; ENSG00000225452
ENST00000432616; ENSP00000413131; ENSG00000183574
ENST00000445885; ENSP00000401172; ENSG00000227277
ENST00000449794; ENSP00000415784; ENSG00000223767
ENST00000453473; ENSP00000415127; ENSG00000228907
ENST00000456167; ENSP00000388795; ENSG00000228405
GeneIDi6048
KEGGihsa:6048
UCSCiuc003oaj.5 human

Similar proteinsi

Entry informationi

Entry nameiRNF5_HUMAN
AccessioniPrimary (citable) accession number: Q99942
Secondary accession number(s): A2BFI6
, B2R4K3, Q0VDB7, Q9UMQ2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: May 1, 1997
Last modified: July 18, 2018
This is version 170 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways

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