UniProtKB - Q99933 (BAG1_HUMAN)
BAG family molecular chaperone regulator 1
BAG1
Functioni
Co-chaperone for HSP70 and HSC70 chaperone proteins. Acts as a nucleotide-exchange factor (NEF) promoting the release of ADP from the HSP70 and HSC70 proteins thereby triggering client/substrate protein release. Nucleotide release is mediated via its binding to the nucleotide-binding domain (NBD) of HSPA8/HSC70 where as the substrate release is mediated via its binding to the substrate-binding domain (SBD) of HSPA8/HSC70 (PubMed:27474739, PubMed:9873016, PubMed:24318877).
Inhibits the pro-apoptotic function of PPP1R15A, and has anti-apoptotic activity (PubMed:12724406).
Markedly increases the anti-cell death function of BCL2 induced by various stimuli (PubMed:9305631).
5 PublicationsGO - Molecular functioni
- adenyl-nucleotide exchange factor activity Source: UniProtKB
- chaperone binding Source: GO_Central
- ubiquitin protein ligase binding Source: ARUK-UCL
GO - Biological processi
- apoptotic process Source: UniProtKB-KW
- cell surface receptor signaling pathway Source: ProtInc
- chaperone cofactor-dependent protein refolding Source: UniProtKB
- negative regulation of apoptotic process Source: ProtInc
- protein stabilization Source: GO_Central
Keywordsi
Molecular function | Chaperone |
Biological process | Apoptosis |
Enzyme and pathway databases
PathwayCommonsi | Q99933 |
Reactomei | R-HSA-3371453, Regulation of HSF1-mediated heat shock response |
SignaLinki | Q99933 |
SIGNORi | Q99933 |
Names & Taxonomyi
Protein namesi | Recommended name: BAG family molecular chaperone regulator 1Short name: BAG-1 Alternative name(s): Bcl-2-associated athanogene 1 |
Gene namesi | Name:BAG1 Synonyms:HAP |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:937, BAG1 |
MIMi | 601497, gene |
neXtProti | NX_Q99933 |
VEuPathDBi | HostDB:ENSG00000107262 |
Subcellular locationi
Cytoplasm and Cytosol
Nucleus
Note: Isoform 4 localizes predominantly to the cytoplasm. The cellular background in which it is expressed can influence whether it resides primarily in the cytoplasm or is also found in the nucleus. In the presence of BCL2, localizes to intracellular membranes (what appears to be the nuclear envelope and perinuclear membranes) as well as punctate cytosolic structures suggestive of mitochondria.
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 308 – 309 | RK → AA: Significant loss of interaction with HSPA8. 1 Publication | 2 |
Organism-specific databases
DisGeNETi | 573 |
OpenTargetsi | ENSG00000107262 |
PharmGKBi | PA25237 |
Miscellaneous databases
Pharosi | Q99933, Tbio |
Chemistry databases
DrugBanki | DB07045, (2R,3R,4S,5R)-2-[6-amino-8-[(3,4-dichlorophenyl)methylamino]purin-9-yl]-5-(hydroxymethyl)oxolane-3,4-diol |
Genetic variation databases
BioMutai | BAG1 |
DMDMi | 296439462 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000088865 | 1 – 345 | BAG family molecular chaperone regulator 1Add BLAST | 345 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 223 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q99933 |
jPOSTi | Q99933 |
MassIVEi | Q99933 |
MaxQBi | Q99933 |
PaxDbi | Q99933 |
PeptideAtlasi | Q99933 |
PRIDEi | Q99933 |
ProteomicsDBi | 78526 [Q99933-1] 78527 [Q99933-2] 78528 [Q99933-3] 78529 [Q99933-4] |
PTM databases
GlyGeni | Q99933, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q99933 |
PhosphoSitePlusi | Q99933 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
Bgeei | ENSG00000107262, Expressed in palpebral conjunctiva and 245 other tissues |
ExpressionAtlasi | Q99933, baseline and differential |
Genevisiblei | Q99933, HS |
Organism-specific databases
HPAi | ENSG00000107262, Low tissue specificity |
Interactioni
Subunit structurei
Homodimer.
Forms a heteromeric complex with HSP70/HSC70 (PubMed:9305631). Binds to the ATPase domain of HSP/HSC70 chaperones. Isoform 1, isoform 3 and isoform 4 but not isoform 2 interact with HSPA8/HSC70 (PubMed:27474739, PubMed:24318877, PubMed:9305631, PubMed:9679980).
Interacts with NR3C1 (PubMed:10477749).
Interacts with the N-terminal region of STK19 (PubMed:15986447).
Interacts with PPP1R15A (PubMed:12724406).
Interacts with BCL2 in an ATP-dependent manner. Isoform 2 does not interact with BCL2 (PubMed:9305631).
Interacts with SIAH1 (PubMed:9582267).
Interacts with HSPA8 (via NBD) (PubMed:27474739, PubMed:24318877).
Interacts with HSPA1A (via NBD) and HSPA1B (via NBD) (PubMed:24318877).
Interacts with SIAH2 (By similarity).
By similarity9 PublicationsBinary interactionsi
Q99933
GO - Molecular functioni
- chaperone binding Source: GO_Central
- ubiquitin protein ligase binding Source: ARUK-UCL
Protein-protein interaction databases
BioGRIDi | 107049, 154 interactors |
CORUMi | Q99933 |
DIPi | DIP-3341N |
IntActi | Q99933, 57 interactors |
MINTi | Q99933 |
STRINGi | 9606.ENSP00000420514 |
Miscellaneous databases
RNActi | Q99933, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q99933 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q99933 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 96 – 101 | 1 | 6 | |
Repeati | 102 – 107 | 2 | 6 | |
Repeati | 108 – 113 | 3 | 6 | |
Repeati | 114 – 119 | 4 | 6 | |
Repeati | 120 – 125 | 5 | 6 | |
Repeati | 126 – 131 | 6 | 6 | |
Repeati | 132 – 137 | 7 | 6 | |
Domaini | 144 – 224 | Ubiquitin-likePROSITE-ProRule annotationAdd BLAST | 81 | |
Domaini | 246 – 326 | BAGPROSITE-ProRule annotationAdd BLAST | 81 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 137 | DisorderedSequence analysisAdd BLAST | 137 | |
Regioni | 96 – 137 | 7 X 6 AA tandem repeat of E-E-X(4)Add BLAST | 42 | |
Regioni | 172 – 219 | Interaction with HSPA8Add BLAST | 48 | |
Regioni | 216 – 345 | Interaction with PPP1R15A1 PublicationAdd BLAST | 130 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 7 – 29 | Basic and acidic residuesSequence analysisAdd BLAST | 23 | |
Compositional biasi | 98 – 113 | Polar residuesSequence analysisAdd BLAST | 16 | |
Compositional biasi | 114 – 137 | Basic and acidic residuesSequence analysisAdd BLAST | 24 |
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | ENOG502RN5W, Eukaryota |
GeneTreei | ENSGT00450000040296 |
HOGENOMi | CLU_055378_0_1_1 |
InParanoidi | Q99933 |
OMAi | NFSDCRM |
OrthoDBi | 1450573at2759 |
PhylomeDBi | Q99933 |
Family and domain databases
Gene3Di | 1.20.58.120, 1 hit |
IDEALi | IID00495 |
InterProi | View protein in InterPro IPR017093, BAG-1 IPR039773, BAG_chaperone_regulator IPR036533, BAG_dom_sf IPR003103, BAG_domain IPR000626, Ubiquitin-like_dom IPR029071, Ubiquitin-like_domsf |
PANTHERi | PTHR12329, PTHR12329, 1 hit PTHR12329:SF16, PTHR12329:SF16, 1 hit |
Pfami | View protein in Pfam PF02179, BAG, 1 hit PF00240, ubiquitin, 1 hit |
SMARTi | View protein in SMART SM00264, BAG, 1 hit SM00213, UBQ, 1 hit |
SUPFAMi | SSF54236, SSF54236, 1 hit SSF63491, SSF63491, 1 hit |
PROSITEi | View protein in PROSITE PS51035, BAG, 1 hit PS50053, UBIQUITIN_2, 1 hit |
s (4+)i Sequence
Sequence statusi: Complete.
This entry describes 4 produced by isoformsialternative splicing and alternative initiation. AlignAdd to basketThis entry has 4 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAQRGGARRP RGDRERLGSR LRALRPGREP RQSEPPAQRG PPPSGRPPAR
60 70 80 90 100
STASGHDRPT RGAAAGARRP RMKKKTRRRS TRSEELTRSE ELTLSEEATW
110 120 130 140 150
SEEATQSEEA TQGEEMNRSQ EVTRDEESTR SEEVTREEMA AAGLTVTVTH
160 170 180 190 200
SNEKHDLHVT SQQGSSEPVV QDLAQVVEEV IGVPQSFQKL IFKGKSLKEM
210 220 230 240 250
ETPLSALGIQ DGCRVMLIGK KNSPQEEVEL KKLKHLEKSV EKIADQLEEL
260 270 280 290 300
NKELTGIQQG FLPKDLQAEA LCKLDRRVKA TIEQFMKILE EIDTLILPEN
310 320 330 340
FKDSRLKRKG LVKKVQAFLA ECDTVEQNIC QETERLQSTN FALAE
Computationally mapped potential isoform sequencesi
There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketJ3QTA2 | J3QTA2_HUMAN | BAG family molecular chaperone regu... | BAG1 | 345 | Annotation score: | ||
C9JYK5 | C9JYK5_HUMAN | BAG family molecular chaperone regu... | BAG1 | 150 | Annotation score: | ||
C9J9Y9 | C9J9Y9_HUMAN | BAG family molecular chaperone regu... | BAG1 | 48 | Annotation score: | ||
A0A0U1RR89 | A0A0U1RR89_HUMAN | BAG family molecular chaperone regu... | BAG1 | 63 | Annotation score: | ||
A0A0U1RQD7 | A0A0U1RQD7_HUMAN | BAG family molecular chaperone regu... | BAG1 | 94 | Annotation score: | ||
F1LLU6 | F1LLU6_HUMAN | BAG family molecular chaperone regu... | BAG1 | 160 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 45 | G → R in AAC34258 (PubMed:8812483).Curated | 1 | |
Sequence conflicti | 45 | G → R in BAD96469 (Ref. 5) Curated | 1 | |
Sequence conflicti | 45 | G → R in AAH01936 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 45 | G → R in AAH14774 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 79 | R → F in AAD11467 (PubMed:8812483).Curated | 1 | |
Sequence conflicti | 84 | E → K in AAD11467 (PubMed:8812483).Curated | 1 | |
Sequence conflicti | 90 | E → K in AAD11467 (PubMed:8812483).Curated | 1 | |
Sequence conflicti | 245 | D → N in AAD11467 (PubMed:8812483).Curated | 1 | |
Sequence conflicti | 293 | D → H in AAD11467 (PubMed:8812483).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_038394 | 1 – 115 | Missing in isoform 4. CuratedAdd BLAST | 115 | |
Alternative sequenceiVSP_038395 | 1 – 71 | Missing in isoform 3. CuratedAdd BLAST | 71 | |
Alternative sequenceiVSP_000453 | 302 – 345 | KDSRL…FALAE → PTLTLVLNEK in isoform 2. 1 PublicationAdd BLAST | 44 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z35491 mRNA Translation: CAA84624.1 Different initiation. U46917 mRNA Translation: AAD11467.1 Different initiation. AF022224 mRNA Translation: AAC34258.1 AF116273 mRNA Translation: AAD25045.1 Different initiation. AK222749 mRNA Translation: BAD96469.1 Different initiation. AL161445 Genomic DNA No translation available. AL356472 Genomic DNA No translation available. CH471071 Genomic DNA Translation: EAW58514.1 CH471071 Genomic DNA Translation: EAW58515.1 Sequence problems. BC001936 mRNA Translation: AAH01936.2 BC014774 mRNA Translation: AAH14774.2 |
CCDSi | CCDS35004.1 [Q99933-1] CCDS55301.1 [Q99933-4] |
RefSeqi | NP_001165886.1, NM_001172415.1 [Q99933-4] NP_004314.5, NM_004323.5 [Q99933-1] |
Genome annotation databases
Ensembli | ENST00000379704; ENSP00000369026; ENSG00000107262 [Q99933-4] ENST00000634734; ENSP00000489189; ENSG00000107262 |
GeneIDi | 573 |
KEGGi | hsa:573 |
MANE-Selecti | ENST00000634734.3; ENSP00000489189.2; NM_004323.6; NP_004314.6 |
UCSCi | uc064sos.1, human [Q99933-1] |
Keywords - Coding sequence diversityi
Alternative initiation, Alternative splicingSimilar proteinsi
Cross-referencesi
Web resourcesi
Atlas of Genetics and Cytogenetics in Oncology and Haematology |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z35491 mRNA Translation: CAA84624.1 Different initiation. U46917 mRNA Translation: AAD11467.1 Different initiation. AF022224 mRNA Translation: AAC34258.1 AF116273 mRNA Translation: AAD25045.1 Different initiation. AK222749 mRNA Translation: BAD96469.1 Different initiation. AL161445 Genomic DNA No translation available. AL356472 Genomic DNA No translation available. CH471071 Genomic DNA Translation: EAW58514.1 CH471071 Genomic DNA Translation: EAW58515.1 Sequence problems. BC001936 mRNA Translation: AAH01936.2 BC014774 mRNA Translation: AAH14774.2 |
CCDSi | CCDS35004.1 [Q99933-1] CCDS55301.1 [Q99933-4] |
RefSeqi | NP_001165886.1, NM_001172415.1 [Q99933-4] NP_004314.5, NM_004323.5 [Q99933-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1HX1 | X-ray | 1.90 | B | 222-334 | [»] | |
1WXV | NMR | - | A | 144-222 | [»] | |
3FZF | X-ray | 2.20 | B | 222-334 | [»] | |
3FZH | X-ray | 2.00 | B | 222-334 | [»] | |
3FZK | X-ray | 2.10 | B | 222-334 | [»] | |
3FZL | X-ray | 2.20 | B | 222-334 | [»] | |
3FZM | X-ray | 2.30 | B | 222-334 | [»] | |
3LDQ | X-ray | 1.90 | B | 222-334 | [»] | |
3M3Z | X-ray | 2.10 | B | 222-334 | [»] | |
5AQF | X-ray | 1.88 | B/D | 222-334 | [»] | |
5AQG | X-ray | 2.24 | B/D/F | 222-334 | [»] | |
5AQH | X-ray | 2.00 | B | 222-334 | [»] | |
5AQI | X-ray | 1.98 | B/D | 222-334 | [»] | |
5AQJ | X-ray | 1.96 | B/D/F | 222-334 | [»] | |
5AQK | X-ray | 2.09 | B | 222-334 | [»] | |
5AQL | X-ray | 1.69 | B/D | 222-334 | [»] | |
5AQM | X-ray | 1.63 | B/D | 222-334 | [»] | |
5AQN | X-ray | 2.45 | B/D/F | 222-334 | [»] | |
5AQO | X-ray | 2.12 | B/D/F | 222-334 | [»] | |
5AQP | X-ray | 2.08 | B/D/F | 222-334 | [»] | |
5AQQ | X-ray | 2.72 | B/D/F | 222-334 | [»] | |
5AQR | X-ray | 1.91 | B/D/F | 222-334 | [»] | |
5AQS | X-ray | 2.00 | B/D | 222-334 | [»] | |
5AQT | X-ray | 1.90 | B | 222-334 | [»] | |
5AQU | X-ray | 1.92 | B | 222-334 | [»] | |
5AQV | X-ray | 1.75 | B | 222-334 | [»] | |
SMRi | Q99933 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 107049, 154 interactors |
CORUMi | Q99933 |
DIPi | DIP-3341N |
IntActi | Q99933, 57 interactors |
MINTi | Q99933 |
STRINGi | 9606.ENSP00000420514 |
Chemistry databases
DrugBanki | DB07045, (2R,3R,4S,5R)-2-[6-amino-8-[(3,4-dichlorophenyl)methylamino]purin-9-yl]-5-(hydroxymethyl)oxolane-3,4-diol |
PTM databases
GlyGeni | Q99933, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q99933 |
PhosphoSitePlusi | Q99933 |
Genetic variation databases
BioMutai | BAG1 |
DMDMi | 296439462 |
Proteomic databases
EPDi | Q99933 |
jPOSTi | Q99933 |
MassIVEi | Q99933 |
MaxQBi | Q99933 |
PaxDbi | Q99933 |
PeptideAtlasi | Q99933 |
PRIDEi | Q99933 |
ProteomicsDBi | 78526 [Q99933-1] 78527 [Q99933-2] 78528 [Q99933-3] 78529 [Q99933-4] |
Protocols and materials databases
Antibodypediai | 2968, 536 antibodies from 41 providers |
DNASUi | 573 |
Genome annotation databases
Ensembli | ENST00000379704; ENSP00000369026; ENSG00000107262 [Q99933-4] ENST00000634734; ENSP00000489189; ENSG00000107262 |
GeneIDi | 573 |
KEGGi | hsa:573 |
MANE-Selecti | ENST00000634734.3; ENSP00000489189.2; NM_004323.6; NP_004314.6 |
UCSCi | uc064sos.1, human [Q99933-1] |
Organism-specific databases
CTDi | 573 |
DisGeNETi | 573 |
GeneCardsi | BAG1 |
HGNCi | HGNC:937, BAG1 |
HPAi | ENSG00000107262, Low tissue specificity |
MIMi | 601497, gene |
neXtProti | NX_Q99933 |
OpenTargetsi | ENSG00000107262 |
PharmGKBi | PA25237 |
VEuPathDBi | HostDB:ENSG00000107262 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502RN5W, Eukaryota |
GeneTreei | ENSGT00450000040296 |
HOGENOMi | CLU_055378_0_1_1 |
InParanoidi | Q99933 |
OMAi | NFSDCRM |
OrthoDBi | 1450573at2759 |
PhylomeDBi | Q99933 |
Enzyme and pathway databases
PathwayCommonsi | Q99933 |
Reactomei | R-HSA-3371453, Regulation of HSF1-mediated heat shock response |
SignaLinki | Q99933 |
SIGNORi | Q99933 |
Miscellaneous databases
BioGRID-ORCSi | 573, 9 hits in 1052 CRISPR screens |
ChiTaRSi | BAG1, human |
EvolutionaryTracei | Q99933 |
GeneWikii | BAG1 |
GenomeRNAii | 573 |
Pharosi | Q99933, Tbio |
PROi | PR:Q99933 |
RNActi | Q99933, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000107262, Expressed in palpebral conjunctiva and 245 other tissues |
ExpressionAtlasi | Q99933, baseline and differential |
Genevisiblei | Q99933, HS |
Family and domain databases
Gene3Di | 1.20.58.120, 1 hit |
IDEALi | IID00495 |
InterProi | View protein in InterPro IPR017093, BAG-1 IPR039773, BAG_chaperone_regulator IPR036533, BAG_dom_sf IPR003103, BAG_domain IPR000626, Ubiquitin-like_dom IPR029071, Ubiquitin-like_domsf |
PANTHERi | PTHR12329, PTHR12329, 1 hit PTHR12329:SF16, PTHR12329:SF16, 1 hit |
Pfami | View protein in Pfam PF02179, BAG, 1 hit PF00240, ubiquitin, 1 hit |
SMARTi | View protein in SMART SM00264, BAG, 1 hit SM00213, UBQ, 1 hit |
SUPFAMi | SSF54236, SSF54236, 1 hit SSF63491, SSF63491, 1 hit |
PROSITEi | View protein in PROSITE PS51035, BAG, 1 hit PS50053, UBIQUITIN_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | BAG1_HUMAN | |
Accessioni | Q99933Primary (citable) accession number: Q99933 Secondary accession number(s): O75315 Q9Y2V4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | May 18, 2010 | |
Last modified: | February 23, 2022 | |
This is version 211 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 9
Human chromosome 9: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references