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UniProtKB - Q99932 (SPAG8_HUMAN)

Entry version 153 (23 Feb 2022)
Sequence version 3 (16 Jan 2019)
Previous versions | rss
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Protein

Sperm-associated antigen 8

Gene

SPAG8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Plays a role in spermatogenesis by enhancing the binding of CREM isoform tau to its coactivator FHL5 and increasing the FHL5-regulated transcriptional activation of CREM isoform tau (By similarity).

Involved in the acrosome reaction and in binding of sperm to the zona pellucida (By similarity).

Plays a role in regulation of the cell cycle by controlling progression through the G2/M phase, possibly by delaying the activation of CDK1 which is required for entry into mitosis (PubMed:19548270).

May play a role in fertility and microtubule formation through interaction with RANBP9 (PubMed:10500252).

By similarity2 Publications

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Biological processCell cycle, Differentiation, Fertilization, Spermatogenesis

Enzyme and pathway databases

Pathway Commons web resource for biological pathway data

More...PathwayCommonsi		Q99932

SignaLink: a signaling pathway resource with multi-layered regulatory networks

More...SignaLinki		Q99932

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Sperm-associated antigen 8
Alternative name(s):
HSD-1
Sperm membrane protein 1
Short name:
SMP-1
Sperm membrane protein BS-84
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:SPAG8
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiHomo sapiens (Human)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri9606 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000005640 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 9

Organism-specific databases

Human Gene Nomenclature Database

More...HGNCi		HGNC:14105, SPAG8

Online Mendelian Inheritance in Man (OMIM)

More...MIMi		605731, gene

neXtProt; the human protein knowledge platform

More...neXtProti		NX_Q99932

Eukaryotic Pathogen, Vector and Host Database Resources

More...VEuPathDBi		HostDB:ENSG00000137098

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Nucleus

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Organism-specific databases

DisGeNET

More...DisGeNETi		26206

MalaCards human disease database

More...MalaCardsi		SPAG8

Open Targets

More...OpenTargetsi		ENSG00000137098

The Pharmacogenetics and Pharmacogenomics Knowledge Base

More...PharmGKBi		PA37843

Miscellaneous databases

Pharos NIH Druggable Genome Knowledgebase

More...Pharosi		Q99932, Tbio

Genetic variation databases

BioMuta curated single-nucleotide variation and disease association database

More...BioMutai		SPAG8

Domain mapping of disease mutations (DMDM)

More...DMDMi		62511128

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000720991 – 485Sperm-associated antigen 8Add BLAST485

Proteomic databases

jPOST - Japan Proteome Standard Repository/Database

More...jPOSTi		Q99932

MassIVE - Mass Spectrometry Interactive Virtual Environment

More...MassIVEi		Q99932

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		Q99932

PeptideAtlas

More...PeptideAtlasi		Q99932

PRoteomics IDEntifications database

More...PRIDEi		Q99932

ProteomicsDB: a multi-organism proteome resource

More...ProteomicsDBi		19756
78524 [Q99932-1]
78525 [Q99932-2]

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...iPTMneti		Q99932

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

More...PhosphoSitePlusi		Q99932

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified 'at protein level'.<br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

Expressed in testis (germ cells), but not in liver, kidney, prostate and small intestine.1 Publication

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...Bgeei		ENSG00000137098, Expressed in right uterine tube and 224 other tissues

ExpressionAtlas, Differential and Baseline Expression

More...ExpressionAtlasi		Q99932, baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

More...Genevisiblei		Q99932, HS

Organism-specific databases

Human Protein Atlas

More...HPAi		ENSG00000137098, Tissue enhanced (choroid plexus, fallopian tube, testis)

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Interacts with FHL5 (via second LIM domain) (By similarity).

Interacts with RANBP9 (PubMed:15014887).

By similarity1 Publication

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection">Interaction</a>' section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

Q99932
With#Exp.IntAct
CSTF2 [P33240]3EBI-954419,EBI-711360
DTX2 [Q86UW9]3EBI-954419,EBI-740376
KLHL32 [Q5THT1]3EBI-954419,EBI-10247181
SNRPC [Q5TAL4]3EBI-954419,EBI-10246938
Isoform 2 [Q99932-2]
With#Exp.IntAct
APP [P05067]3EBI-11959123,EBI-77613
APP - isoform APP305 [P05067-2]3EBI-11959123,EBI-17264467
ARMC5 [Q96C12]3EBI-11959123,EBI-6425121
ATXN1 [P54253]6EBI-11959123,EBI-930964
ATXN3 [P54252]6EBI-11959123,EBI-946046
B9D2 [Q9BPU9]3EBI-11959123,EBI-6958971
BATF2 [Q8N1L9]3EBI-11959123,EBI-742695
BSCL2 [J3KQ12]3EBI-11959123,EBI-11532900
C19orf54 - isoform 3 [Q5BKX5-3]4EBI-11959123,EBI-11976299
CCDC120 [Q96HB5]3EBI-11959123,EBI-744556
CCNC [P24863]3EBI-11959123,EBI-395261
CCT5 [P48643]3EBI-11959123,EBI-355710
CDC23 [Q9UJX2]3EBI-11959123,EBI-396137
CFP [P27918]4EBI-11959123,EBI-9038570
CISD2 [Q8N5K1]3EBI-11959123,EBI-1045797
CSTF2 [P33240]3EBI-11959123,EBI-711360
CSTF2T [Q9H0L4]3EBI-11959123,EBI-747012
CTAG1B [P78358]5EBI-11959123,EBI-1188472
DCTN1 - isoform 5 [Q14203-5]3EBI-11959123,EBI-25840379
DMRT3 [Q9NQL9]3EBI-11959123,EBI-9679045
DMWD [G5E9A7]3EBI-11959123,EBI-10976677
DOK3 - isoform 3 [Q7L591-3]3EBI-11959123,EBI-10694655
DYNLL2 [Q96FJ2]3EBI-11959123,EBI-742371
EGFL7 [Q9UHF1]3EBI-11959123,EBI-949532
FAM168B - isoform 2 [A1KXE4-2]3EBI-11959123,EBI-12193763
FAM222B [Q8WU58]3EBI-11959123,EBI-2807642
FRS3 [O43559]3EBI-11959123,EBI-725515
GARS1 [P41250]3EBI-11959123,EBI-724143
GIGYF1 [O75420]3EBI-11959123,EBI-947774
GLYCTK [Q8IVS8]3EBI-11959123,EBI-748515
GPSM3 [Q9Y4H4]3EBI-11959123,EBI-347538
HEMK1 [Q9Y5R4]3EBI-11959123,EBI-10329202
HR [O43593]3EBI-11959123,EBI-2880706
HSPB1 [P04792]3EBI-11959123,EBI-352682
HTT [P42858]18EBI-11959123,EBI-466029
INCA1 [Q0VD86]3EBI-11959123,EBI-6509505
INTS11 [Q5TA45]3EBI-11959123,EBI-748258
KIF1B - isoform 2 [O60333-2]3EBI-11959123,EBI-10975473
KRT34 [O76011]3EBI-11959123,EBI-1047093
KRTAP10-8 [P60410]3EBI-11959123,EBI-10171774
KRTAP11-1 [Q8IUC1]3EBI-11959123,EBI-1052037
KRTAP13-3 [Q3SY46]3EBI-11959123,EBI-10241252
KRTAP15-1 [Q3LI76]3EBI-11959123,EBI-11992140
KRTAP19-2 [Q3LHN2]3EBI-11959123,EBI-12196745
KRTAP19-6 [Q3LI70]3EBI-11959123,EBI-12805508
KRTAP19-7 [Q3SYF9]3EBI-11959123,EBI-10241353
KRTAP21-2 [Q3LI59]3EBI-11959123,EBI-18395721
KRTAP26-1 [Q6PEX3]3EBI-11959123,EBI-3957672
KRTAP3-1 [Q9BYR8]3EBI-11959123,EBI-9996449
KRTAP6-2 [Q3LI66]5EBI-11959123,EBI-11962084
KRTAP7-1 [Q8IUC3]3EBI-11959123,EBI-18394498
LASP1 - isoform 2 [Q14847-2]3EBI-11959123,EBI-9088686
MEOX1 [P50221]3EBI-11959123,EBI-2864512
METTL15 - isoform 4 [A6NJ78-4]3EBI-11959123,EBI-10174029
NDRG1 [Q92597]3EBI-11959123,EBI-716486
NDUFV1 [P49821]3EBI-11959123,EBI-748312
NEFL [P07196]3EBI-11959123,EBI-475646
NHLRC4 [P0CG21]3EBI-11959123,EBI-12868744
NUDT21 [O43809]3EBI-11959123,EBI-355720
PATZ1 - isoform 4 [Q9HBE1-4]3EBI-11959123,EBI-11022007
PEF1 [Q9UBV8]3EBI-11959123,EBI-724639
PEX7 - isoform 2 [O00628-2]3EBI-11959123,EBI-25882083
PFDN5 [Q99471]3EBI-11959123,EBI-357275
PHF1 [O43189]3EBI-11959123,EBI-530034
PINK1 [Q9BXM7]3EBI-11959123,EBI-2846068
PITX1 [P78337]4EBI-11959123,EBI-748265
PLA2G10 [O15496]3EBI-11959123,EBI-726466
PPT1 [P50897]3EBI-11959123,EBI-1237011
PRKN - isoform 5 [O60260-5]6EBI-11959123,EBI-21251460
PRPS1 [P60891]3EBI-11959123,EBI-749195
PRR35 [P0CG20]3EBI-11959123,EBI-11986293
PSEN1 - isoform 2 [P49768-2]6EBI-11959123,EBI-11047108
RBP3 [P10745]3EBI-11959123,EBI-12806054
RIPK4 - isoform 2 [P57078-2]3EBI-11959123,EBI-12854608
RNF11 [Q9Y3C5]3EBI-11959123,EBI-396669
RUSC1 [Q9BVN2]3EBI-11959123,EBI-6257312
SERGEF [Q9UGK8]3EBI-11959123,EBI-465368
SMUG1 - isoform 2 [Q53HV7-2]3EBI-11959123,EBI-12275818
SMYD1 [Q8NB12]3EBI-11959123,EBI-8463848
SNCA [P37840]3EBI-11959123,EBI-985879
SNRPB - isoform SM-B [P14678-2]3EBI-11959123,EBI-372475
SNRPC [P09234]3EBI-11959123,EBI-766589
SPRED1 [Q7Z699]3EBI-11959123,EBI-5235340
SYNGAP1 [Q96PV0]3EBI-11959123,EBI-2682386
TARDBP [Q13148]6EBI-11959123,EBI-372899
TEKT3 [Q9BXF9]4EBI-11959123,EBI-8644516
TEKT5 [Q96M29]3EBI-11959123,EBI-10239812
TENT5B [Q96A09]3EBI-11959123,EBI-752030
TRIB3 [Q96RU7]3EBI-11959123,EBI-492476
TRIM42 [Q8IWZ5]5EBI-11959123,EBI-5235829
TSC1 [Q86WV8]6EBI-11959123,EBI-12806590
TTC23 - isoform 3 [Q5W5X9-3]3EBI-11959123,EBI-9090990
UBAP2 [Q5T6F2]3EBI-11959123,EBI-2514383
UBQLN1 [Q9UMX0]3EBI-11959123,EBI-741480
USP2 [O75604]3EBI-11959123,EBI-743272
VPS37C [A5D8V6]3EBI-11959123,EBI-2559305
WFS1 [O76024]3EBI-11959123,EBI-720609
YARS1 [P54577]3EBI-11959123,EBI-1048893
ZBTB32 [A0A0C4DGF1]7EBI-11959123,EBI-10188476
ZIC1 [Q15915]5EBI-11959123,EBI-11963196
ZNF581 [Q9P0T4]3EBI-11959123,EBI-745520

GO - Molecular functioni

Complete GO annotation on QuickGO ...

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...BioGRIDi		117606, 89 interactors

Protein interaction database and analysis system

More...IntActi		Q99932, 111 interactors

Molecular INTeraction database

More...MINTi		Q99932

STRING: functional protein association networks

More...STRINGi		9606.ENSP00000340982

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...RNActi		Q99932, protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni1 – 42DisorderedSequence analysisAdd BLAST42
Regioni75 – 99DisorderedSequence analysisAdd BLAST25
Regioni127 – 215DisorderedSequence analysisAdd BLAST89

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes the position of regions of compositional bias within the protein and the particular type of amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi13 – 37Polar residuesSequence analysisAdd BLAST25
Compositional biasi127 – 157Polar residuesSequence analysisAdd BLAST31

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the SPAG8 family.Curated

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		ENOG502S06E, Eukaryota

Ensembl GeneTree

More...GeneTreei		ENSGT00640000091617

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		CLU_052284_0_0_1

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		Q99932

Identification of Orthologs from Complete Genome Data

More...OMAi		WKPPEVE

Database of Orthologous Groups

More...OrthoDBi		1153567at2759

Database for complete collections of gene phylogenies

More...PhylomeDBi		Q99932

TreeFam database of animal gene trees

More...TreeFami		TF329075

Family and domain databases

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR026124, Sperm-assoc_Ag8

The PANTHER Classification System

More...PANTHERi		PTHR15510, PTHR15510, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (3+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry describes 3 <p>This subsection of the 'Sequence' section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All

Isoform 3 (identifier: Q99932-3) [UniParc]FASTAAdd to basket

This isoform has been chosen as the <p><strong>What is the canonical sequence?</strong><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
METNESTEGS RSRSRSLDIQ PSSEGLGPTS EPFPSSDDSP RSALAAATAA 
        60         70         80         90        100
AAAAASAAAA TAAFTTAKAA ALSTKTPAPC SEFMEPSSDP SLLGEPCAGP 
       110        120        130        140        150
GFTHNIAHGS LGFEPVYVSC IAQDTCTTTD HSSNPGPVPG SSSGPVLGSS 
       160        170        180        190        200
SGAGHGSGSG SGPGCGSVPG SGSGPGPGSG PGSGPGHGSG SHPGPASGPG 
       210        220        230        240        250
PDTGPDSELS PCIPPGFRNL VADRVPNYTS WSQHCPWEPQ KQPPWEFLQV 
       260        270        280        290        300
LEPGARGLWK PPDIKGKLMV CYETLPRGQC LLYNWEEERA TNHLDQVPSM 
       310        320        330        340        350
QDGSESFFFR HGHRGLLTMQ LKSPMPSSTT QKDSYQPPGN VYWPLRGKRE 
       360        370        380        390        400
AMLEMLLQHQ ICKEVQAEQE PTRKLFEVES VTHHDYRMEL AQAGTPAPTK 
       410        420        430        440        450
PHDYRQEQPE TFWIQRAPQL PGVSNIRTLD TPFRKNCSFS TPVPLSLGKL 
       460        470        480 
LPYEPENYPY QLGEISSLPC PGGRLGGGGG RMTPF
Length:485
Mass (Da):51,139
Last modified:January 16, 2019 - v3
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i206A2AE68C5A4887
GO
Isoform 1 (identifier: Q99932-1) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     422-426: GVSNI → VCEGD
     427-485: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.Curated
Show »
Length:426
Mass (Da):44,819
Checksum:i7CFF3447202CA270
GO
Isoform 2 (identifier: Q99932-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     422-485: GVSNIRTLDT...GGGGGRMTPF → TWWPLPTQVP...KTVTQEDRGW

Show »
Length:501
Mass (Da):53,507
Checksum:iF7A14ED49DF6CE03
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
C9JAA8C9JAA8_HUMAN
Sperm-associated antigen 8
SPAG8
477Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
H7C4J6H7C4J6_HUMAN
Sperm-associated antigen 8
SPAG8
483Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
F8WBP3F8WBP3_HUMAN
Sperm-associated antigen 8
SPAG8
96Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
F2Z302F2Z302_HUMAN
Sperm-associated antigen 8
SPAG8
69Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

<p>This subsection of the 'Sequence' section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

The sequence AAB46833 differs from that shown. Reason: Erroneous termination. Extended C-terminus.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti5E → G in AAH19247 (PubMed:15489334).Curated1
Sequence conflicti48T → A in BAG63651 (PubMed:14702039).Curated1
Sequence conflicti49A → S in AAB46833 (PubMed:8788182).Curated1
Sequence conflicti176 – 179Missing in BAG63651 (PubMed:14702039).Curated4
Sequence conflicti269M → Q in AAB46833 (PubMed:8788182).Curated1
Sequence conflicti322K → T in AAB46833 (PubMed:8788182).Curated1
Sequence conflicti438S → G in BAG63651 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes natural variant(s) of the protein sequence.<p><a href='/help/variant' target='_top'>More...</a></p>Natural variantiVAR_05699269A → T. Corresponds to variant dbSNP:rs13299596Ensembl.1
Natural variantiVAR_069166226P → L1 PublicationCorresponds to variant dbSNP:rs17851728Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_059990422 – 485GVSNI…RMTPF → TWWPLPTQVPAAEDYLTWKE WGFTGVQEVLSALLRATPGE YSVNICGMNEHPVCSRTWTN RLCHQEMGSKKTVTQEDRGW in isoform 2. Add BLAST64
Alternative sequenceiVSP_059991422 – 426GVSNI → VCEGD in isoform 1. 5
Alternative sequenceiVSP_059992427 – 485Missing in isoform 1. Add BLAST59

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
S83157 mRNA Translation: AAB46833.1 Sequence problems.
U12978 mRNA Translation: AAA21129.2
AK302313 mRNA Translation: BAG63651.1
AL133410 Genomic DNA No translation available.
BC019247 mRNA Translation: AAH19247.1

The Consensus CDS (CCDS) project

More...CCDSi		CCDS43798.1 [Q99932-3]
CCDS6592.1 [Q99932-2]

NCBI Reference Sequences

More...RefSeqi		NP_001034681.1, NM_001039592.1 [Q99932-3]
NP_758516.1, NM_172312.1 [Q99932-2]

Genome annotation databases

Ensembl eukaryotic genome annotation project

More...Ensembli		ENST00000340291; ENSP00000340982; ENSG00000137098 [Q99932-2]
ENST00000396638; ENSP00000379878; ENSG00000137098
ENST00000475644; ENSP00000418530; ENSG00000137098 [Q99932-1]

Database of genes from NCBI RefSeq genomes

More...GeneIDi		26206

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		hsa:26206

Matched Annotation from NCBI and EMBL-EBI (MANE) - Phase one

More...MANE-Selecti		ENST00000396638.7; ENSP00000379878.2; NM_001039592.2; NP_001034681.1

UCSC genome browser

More...UCSCi		uc003zye.4, human [Q99932-3]

Keywords - Coding sequence diversityi

Alternative splicing

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S83157 mRNA Translation: AAB46833.1 Sequence problems.
U12978 mRNA Translation: AAA21129.2
AK302313 mRNA Translation: BAG63651.1
AL133410 Genomic DNA No translation available.
BC019247 mRNA Translation: AAH19247.1
CCDSiCCDS43798.1 [Q99932-3]
CCDS6592.1 [Q99932-2]
RefSeqiNP_001034681.1, NM_001039592.1 [Q99932-3]
NP_758516.1, NM_172312.1 [Q99932-2]

3D structure databases

Database of comparative protein structure models

More...ModBasei		Search...

SWISS-MODEL Interactive Workspace

More...SWISS-MODEL-Workspacei		Submit a new modelling project...

Protein-protein interaction databases

BioGRIDi117606, 89 interactors
IntActiQ99932, 111 interactors
MINTiQ99932
STRINGi9606.ENSP00000340982

PTM databases

iPTMnetiQ99932
PhosphoSitePlusiQ99932

Genetic variation databases

BioMutaiSPAG8
DMDMi62511128

Proteomic databases

jPOSTiQ99932
MassIVEiQ99932
PaxDbiQ99932
PeptideAtlasiQ99932
PRIDEiQ99932
ProteomicsDBi19756
78524 [Q99932-1]
78525 [Q99932-2]

Protocols and materials databases

Antibodypedia a portal for validated antibodies

More...Antibodypediai		26087, 223 antibodies from 26 providers

The DNASU plasmid repository

More...DNASUi		26206

Genome annotation databases

EnsembliENST00000340291; ENSP00000340982; ENSG00000137098 [Q99932-2]
ENST00000396638; ENSP00000379878; ENSG00000137098
ENST00000475644; ENSP00000418530; ENSG00000137098 [Q99932-1]
GeneIDi26206
KEGGihsa:26206
MANE-SelectiENST00000396638.7; ENSP00000379878.2; NM_001039592.2; NP_001034681.1
UCSCiuc003zye.4, human [Q99932-3]

Organism-specific databases

Comparative Toxicogenomics Database

More...CTDi		26206
DisGeNETi26206

GeneCards: human genes, protein and diseases

More...GeneCardsi		SPAG8
HGNCiHGNC:14105, SPAG8
HPAiENSG00000137098, Tissue enhanced (choroid plexus, fallopian tube, testis)
MalaCardsiSPAG8
MIMi605731, gene
neXtProtiNX_Q99932
OpenTargetsiENSG00000137098
PharmGKBiPA37843
VEuPathDBiHostDB:ENSG00000137098

GenAtlas: human gene database

More...GenAtlasi		Search...

Phylogenomic databases

eggNOGiENOG502S06E, Eukaryota
GeneTreeiENSGT00640000091617
HOGENOMiCLU_052284_0_0_1
InParanoidiQ99932
OMAiWKPPEVE
OrthoDBi1153567at2759
PhylomeDBiQ99932
TreeFamiTF329075

Enzyme and pathway databases

PathwayCommonsiQ99932
SignaLinkiQ99932

Miscellaneous databases

BioGRID ORCS database of CRISPR phenotype screens

More...BioGRID-ORCSi		26206, 1 hit in 1030 CRISPR screens

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

More...ChiTaRSi		SPAG8, human

The Gene Wiki collection of pages on human genes and proteins

More...GeneWikii		SPAG8

Database of phenotypes from RNA interference screens in Drosophila and Homo sapiens

More...GenomeRNAii		26206
PharosiQ99932, Tbio

Protein Ontology

More...PROi		PR:Q99932
RNActiQ99932, protein

The Stanford Online Universal Resource for Clones and ESTs

More...SOURCEi		Search...

Gene expression databases

BgeeiENSG00000137098, Expressed in right uterine tube and 224 other tissues
ExpressionAtlasiQ99932, baseline and differential
GenevisibleiQ99932, HS

Family and domain databases

InterProiView protein in InterPro
IPR026124, Sperm-assoc_Ag8
PANTHERiPTHR15510, PTHR15510, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiSPAG8_HUMAN
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q99932
Secondary accession number(s): B4DY89
, E9PDV6, Q12937, Q5TCV8, Q8WWB4
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: January 16, 2019
Last modified: February 23, 2022
This is version 153 of the entry and version 3 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
  2. Human entries with genetic variants
    List of human entries with genetic variants
  3. Human variants curated from literature reports
    Index of human variants curated from literature reports
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families
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