UniProtKB - Q99932 (SPAG8_HUMAN)
Sperm-associated antigen 8
SPAG8
Functioni
Plays a role in spermatogenesis by enhancing the binding of CREM isoform tau to its coactivator FHL5 and increasing the FHL5-regulated transcriptional activation of CREM isoform tau (By similarity).
Involved in the acrosome reaction and in binding of sperm to the zona pellucida (By similarity).
Plays a role in regulation of the cell cycle by controlling progression through the G2/M phase, possibly by delaying the activation of CDK1 which is required for entry into mitosis (PubMed:19548270).
May play a role in fertility and microtubule formation through interaction with RANBP9 (PubMed:10500252).
By similarity2 PublicationsGO - Molecular functioni
- microtubule binding Source: InterPro
GO - Biological processi
- cell cycle Source: UniProtKB-KW
- cell differentiation Source: UniProtKB-KW
- positive regulation of protein binding Source: Ensembl
- positive regulation of transcription by RNA polymerase II Source: GO_Central
- single fertilization Source: UniProtKB-KW
- spermatogenesis Source: UniProtKB-KW
Keywordsi
Biological process | Cell cycle, Differentiation, Fertilization, Spermatogenesis |
Enzyme and pathway databases
PathwayCommonsi | Q99932 |
SignaLinki | Q99932 |
Names & Taxonomyi
Protein namesi | Recommended name: Sperm-associated antigen 8Alternative name(s): HSD-1 Sperm membrane protein 1 Short name: SMP-1 Sperm membrane protein BS-84 |
Gene namesi | Name:SPAG8 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:14105, SPAG8 |
MIMi | 605731, gene |
neXtProti | NX_Q99932 |
VEuPathDBi | HostDB:ENSG00000137098 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Cytoskeleton
- microtubule organizing center 1 Publication
- spindle 1 Publication
Nucleus
- Nucleus By similarity
Other locations
- acrosome 3 Publications
Note: In mature sperm cells, detected in the acrosomal region of the head and in the middle piece of the tail (By similarity). Localized to the nucleus and cytoplasm of spermatocytes and round spermatids while, in elongating spermatids, expressed in the cytoplasm but not in the nucleus (By similarity). During the cell cycle, localized on the microtubule-organizing center (MTOC) during prophase. In metaphase, extends along spindle microtubules. In anaphase, detected on the astral microtubules and mid-zone. In telophase, remains at the mid-zone. After cytokinesis, returns to the MTOC (PubMed:19548270).By similarity1 Publication
Cytoskeleton
- spindle Source: UniProtKB-SubCell
Nucleus
- nucleus Source: GO_Central
Other locations
- acrosomal vesicle Source: UniProtKB-SubCell
- cytoplasm Source: GO_Central
- membrane Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 26206 |
MalaCardsi | SPAG8 |
OpenTargetsi | ENSG00000137098 |
PharmGKBi | PA37843 |
Miscellaneous databases
Pharosi | Q99932, Tbio |
Genetic variation databases
BioMutai | SPAG8 |
DMDMi | 62511128 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000072099 | 1 – 485 | Sperm-associated antigen 8Add BLAST | 485 |
Proteomic databases
jPOSTi | Q99932 |
MassIVEi | Q99932 |
PaxDbi | Q99932 |
PeptideAtlasi | Q99932 |
PRIDEi | Q99932 |
ProteomicsDBi | 19756 78524 [Q99932-1] 78525 [Q99932-2] |
PTM databases
iPTMneti | Q99932 |
PhosphoSitePlusi | Q99932 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000137098, Expressed in right uterine tube and 224 other tissues |
ExpressionAtlasi | Q99932, baseline and differential |
Genevisiblei | Q99932, HS |
Organism-specific databases
HPAi | ENSG00000137098, Tissue enhanced (choroid plexus, fallopian tube, testis) |
Interactioni
Subunit structurei
Interacts with FHL5 (via second LIM domain) (By similarity).
Interacts with RANBP9 (PubMed:15014887).
By similarity1 PublicationBinary interactionsi
Q99932
With | #Exp. | IntAct |
---|---|---|
CSTF2 [P33240] | 3 | EBI-954419,EBI-711360 |
DTX2 [Q86UW9] | 3 | EBI-954419,EBI-740376 |
KLHL32 [Q5THT1] | 3 | EBI-954419,EBI-10247181 |
SNRPC [Q5TAL4] | 3 | EBI-954419,EBI-10246938 |
Isoform 2 [Q99932-2]
GO - Molecular functioni
- microtubule binding Source: InterPro
Protein-protein interaction databases
BioGRIDi | 117606, 89 interactors |
IntActi | Q99932, 111 interactors |
MINTi | Q99932 |
STRINGi | 9606.ENSP00000340982 |
Miscellaneous databases
RNActi | Q99932, protein |
Structurei
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 42 | DisorderedSequence analysisAdd BLAST | 42 | |
Regioni | 75 – 99 | DisorderedSequence analysisAdd BLAST | 25 | |
Regioni | 127 – 215 | DisorderedSequence analysisAdd BLAST | 89 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 13 – 37 | Polar residuesSequence analysisAdd BLAST | 25 | |
Compositional biasi | 127 – 157 | Polar residuesSequence analysisAdd BLAST | 31 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | ENOG502S06E, Eukaryota |
GeneTreei | ENSGT00640000091617 |
HOGENOMi | CLU_052284_0_0_1 |
InParanoidi | Q99932 |
OMAi | WKPPEVE |
OrthoDBi | 1153567at2759 |
PhylomeDBi | Q99932 |
TreeFami | TF329075 |
Family and domain databases
InterProi | View protein in InterPro IPR026124, Sperm-assoc_Ag8 |
PANTHERi | PTHR15510, PTHR15510, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
METNESTEGS RSRSRSLDIQ PSSEGLGPTS EPFPSSDDSP RSALAAATAA
60 70 80 90 100
AAAAASAAAA TAAFTTAKAA ALSTKTPAPC SEFMEPSSDP SLLGEPCAGP
110 120 130 140 150
GFTHNIAHGS LGFEPVYVSC IAQDTCTTTD HSSNPGPVPG SSSGPVLGSS
160 170 180 190 200
SGAGHGSGSG SGPGCGSVPG SGSGPGPGSG PGSGPGHGSG SHPGPASGPG
210 220 230 240 250
PDTGPDSELS PCIPPGFRNL VADRVPNYTS WSQHCPWEPQ KQPPWEFLQV
260 270 280 290 300
LEPGARGLWK PPDIKGKLMV CYETLPRGQC LLYNWEEERA TNHLDQVPSM
310 320 330 340 350
QDGSESFFFR HGHRGLLTMQ LKSPMPSSTT QKDSYQPPGN VYWPLRGKRE
360 370 380 390 400
AMLEMLLQHQ ICKEVQAEQE PTRKLFEVES VTHHDYRMEL AQAGTPAPTK
410 420 430 440 450
PHDYRQEQPE TFWIQRAPQL PGVSNIRTLD TPFRKNCSFS TPVPLSLGKL
460 470 480
LPYEPENYPY QLGEISSLPC PGGRLGGGGG RMTPF
The sequence of this isoform differs from the canonical sequence as follows:
422-426: GVSNI → VCEGD
427-485: Missing.
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketC9JAA8 | C9JAA8_HUMAN | Sperm-associated antigen 8 | SPAG8 | 477 | Annotation score: | ||
H7C4J6 | H7C4J6_HUMAN | Sperm-associated antigen 8 | SPAG8 | 483 | Annotation score: | ||
F8WBP3 | F8WBP3_HUMAN | Sperm-associated antigen 8 | SPAG8 | 96 | Annotation score: | ||
F2Z302 | F2Z302_HUMAN | Sperm-associated antigen 8 | SPAG8 | 69 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 5 | E → G in AAH19247 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 48 | T → A in BAG63651 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 49 | A → S in AAB46833 (PubMed:8788182).Curated | 1 | |
Sequence conflicti | 176 – 179 | Missing in BAG63651 (PubMed:14702039).Curated | 4 | |
Sequence conflicti | 269 | M → Q in AAB46833 (PubMed:8788182).Curated | 1 | |
Sequence conflicti | 322 | K → T in AAB46833 (PubMed:8788182).Curated | 1 | |
Sequence conflicti | 438 | S → G in BAG63651 (PubMed:14702039).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_056992 | 69 | A → T. Corresponds to variant dbSNP:rs13299596Ensembl. | 1 | |
Natural variantiVAR_069166 | 226 | P → L1 PublicationCorresponds to variant dbSNP:rs17851728Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_059990 | 422 – 485 | GVSNI…RMTPF → TWWPLPTQVPAAEDYLTWKE WGFTGVQEVLSALLRATPGE YSVNICGMNEHPVCSRTWTN RLCHQEMGSKKTVTQEDRGW in isoform 2. Add BLAST | 64 | |
Alternative sequenceiVSP_059991 | 422 – 426 | GVSNI → VCEGD in isoform 1. | 5 | |
Alternative sequenceiVSP_059992 | 427 – 485 | Missing in isoform 1. Add BLAST | 59 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S83157 mRNA Translation: AAB46833.1 Sequence problems. U12978 mRNA Translation: AAA21129.2 AK302313 mRNA Translation: BAG63651.1 AL133410 Genomic DNA No translation available. BC019247 mRNA Translation: AAH19247.1 |
CCDSi | CCDS43798.1 [Q99932-3] CCDS6592.1 [Q99932-2] |
RefSeqi | NP_001034681.1, NM_001039592.1 [Q99932-3] NP_758516.1, NM_172312.1 [Q99932-2] |
Genome annotation databases
Ensembli | ENST00000340291; ENSP00000340982; ENSG00000137098 [Q99932-2] ENST00000396638; ENSP00000379878; ENSG00000137098 ENST00000475644; ENSP00000418530; ENSG00000137098 [Q99932-1] |
GeneIDi | 26206 |
KEGGi | hsa:26206 |
MANE-Selecti | ENST00000396638.7; ENSP00000379878.2; NM_001039592.2; NP_001034681.1 |
UCSCi | uc003zye.4, human [Q99932-3] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S83157 mRNA Translation: AAB46833.1 Sequence problems. U12978 mRNA Translation: AAA21129.2 AK302313 mRNA Translation: BAG63651.1 AL133410 Genomic DNA No translation available. BC019247 mRNA Translation: AAH19247.1 |
CCDSi | CCDS43798.1 [Q99932-3] CCDS6592.1 [Q99932-2] |
RefSeqi | NP_001034681.1, NM_001039592.1 [Q99932-3] NP_758516.1, NM_172312.1 [Q99932-2] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 117606, 89 interactors |
IntActi | Q99932, 111 interactors |
MINTi | Q99932 |
STRINGi | 9606.ENSP00000340982 |
PTM databases
iPTMneti | Q99932 |
PhosphoSitePlusi | Q99932 |
Genetic variation databases
BioMutai | SPAG8 |
DMDMi | 62511128 |
Proteomic databases
jPOSTi | Q99932 |
MassIVEi | Q99932 |
PaxDbi | Q99932 |
PeptideAtlasi | Q99932 |
PRIDEi | Q99932 |
ProteomicsDBi | 19756 78524 [Q99932-1] 78525 [Q99932-2] |
Protocols and materials databases
Antibodypediai | 26087, 223 antibodies from 26 providers |
DNASUi | 26206 |
Genome annotation databases
Ensembli | ENST00000340291; ENSP00000340982; ENSG00000137098 [Q99932-2] ENST00000396638; ENSP00000379878; ENSG00000137098 ENST00000475644; ENSP00000418530; ENSG00000137098 [Q99932-1] |
GeneIDi | 26206 |
KEGGi | hsa:26206 |
MANE-Selecti | ENST00000396638.7; ENSP00000379878.2; NM_001039592.2; NP_001034681.1 |
UCSCi | uc003zye.4, human [Q99932-3] |
Organism-specific databases
CTDi | 26206 |
DisGeNETi | 26206 |
GeneCardsi | SPAG8 |
HGNCi | HGNC:14105, SPAG8 |
HPAi | ENSG00000137098, Tissue enhanced (choroid plexus, fallopian tube, testis) |
MalaCardsi | SPAG8 |
MIMi | 605731, gene |
neXtProti | NX_Q99932 |
OpenTargetsi | ENSG00000137098 |
PharmGKBi | PA37843 |
VEuPathDBi | HostDB:ENSG00000137098 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502S06E, Eukaryota |
GeneTreei | ENSGT00640000091617 |
HOGENOMi | CLU_052284_0_0_1 |
InParanoidi | Q99932 |
OMAi | WKPPEVE |
OrthoDBi | 1153567at2759 |
PhylomeDBi | Q99932 |
TreeFami | TF329075 |
Enzyme and pathway databases
PathwayCommonsi | Q99932 |
SignaLinki | Q99932 |
Miscellaneous databases
BioGRID-ORCSi | 26206, 1 hit in 1030 CRISPR screens |
ChiTaRSi | SPAG8, human |
GeneWikii | SPAG8 |
GenomeRNAii | 26206 |
Pharosi | Q99932, Tbio |
PROi | PR:Q99932 |
RNActi | Q99932, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000137098, Expressed in right uterine tube and 224 other tissues |
ExpressionAtlasi | Q99932, baseline and differential |
Genevisiblei | Q99932, HS |
Family and domain databases
InterProi | View protein in InterPro IPR026124, Sperm-assoc_Ag8 |
PANTHERi | PTHR15510, PTHR15510, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SPAG8_HUMAN | |
Accessioni | Q99932Primary (citable) accession number: Q99932 Secondary accession number(s): B4DY89 Q8WWB4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 12, 2005 |
Last sequence update: | January 16, 2019 | |
Last modified: | February 23, 2022 | |
This is version 153 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 9
Human chromosome 9: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families