PRMT1

Functionⁱ

Arginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, PIAS1, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15, EWS, HABP4 and SERBP1 (PubMed:16879614, PubMed:26876602). Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 'Arg-4' (H4R3me1 and H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation. Together with dimethylated PIAS1, represses STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. May be involved in the regulation of TAF15 transcriptional activity, act as an activator of estrogen receptor (ER)-mediated transactivation, play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation, by modulating p38 MAPK pathway. Methylates RBM15, promoting ubiquitination and degradation of RBM15 (PubMed:26575292). Methylates FOXO1 and retains it in the nucleus increasing its transcriptional activity. Methylates CHTOP and this methylation is critical for its 5-hydroxymethylcytosine (5hmC)-binding activity (PubMed:25284789). Methylates H4R3 in genes involved in glioblastomagenesis in a CHTOP- and/or TET1-dependent manner (PubMed:25284789).15 Publications

Catalytic activityⁱ

2 S-adenosyl-L-methionine + [protein]-L-arginine = 2 S-adenosyl-L-homocysteine + [protein]-N(omega),N(omega)-dimethyl-L-arginine.5 Publications

Activity regulationⁱ

By BTG1, BTG2 and ILF3.

Kineticsⁱ

Sites

Feature key	Position(s)	DescriptionActions	Length
Binding siteⁱ	63	S-adenosyl-L-methionineBy similarity	1
Binding siteⁱ	72	S-adenosyl-L-methionineBy similarity	1
Binding siteⁱ	96	S-adenosyl-L-methionine; via carbonyl oxygenBy similarity	1
Binding siteⁱ	118	S-adenosyl-L-methionineBy similarity	1
Binding siteⁱ	147	S-adenosyl-L-methionineBy similarity	1
Active siteⁱ	162	By similarity	1
Active siteⁱ	171	By similarity	1

GO - Molecular functionⁱ

enzyme binding
Source: UniProtKB
Inferred from physical interactionⁱ
- 19460357
histone methyltransferase activity
Source: UniProtKB
Inferred from direct assayⁱ
- 19405910
histone methyltransferase activity (H4-R3 specific)
Source: UniProtKB
Inferred from direct assayⁱ
- 11448779
identical protein binding
Source: UniProtKB
Inferred from direct assayⁱ
- 26876602
methyl-CpG binding
Source: UniProtKB
Inferred from direct assayⁱ
- 25284789
methyltransferase activity
Source: ProtInc
Traceable author statementⁱ
- 9545638
mitogen-activated protein kinase p38 binding
Source: BHF-UCL
Inferred from physical interactionⁱ
- 23483889
N-methyltransferase activity
Source: UniProtKB
Inferred from direct assayⁱ
- 18657504
protein-arginine N-methyltransferase activity
Source: MGI
Inferred from direct assayⁱ
- 18495660
protein-arginine omega-N asymmetric methyltransferase activity
Source: UniProtKB
Inferred from direct assayⁱ
- 26575292
protein-arginine omega-N monomethyltransferase activity
Source: UniProtKB
Inferred from direct assayⁱ
- 26575292
protein methyltransferase activity
Source: UniProtKB
Inferred from direct assayⁱ
- 19460357
RNA binding
Source: UniProtKB
Inferred from high throughput direct assayⁱ
- 22681889
S-adenosyl-L-methionine binding
Source: UniProtKB
Inferred from direct assayⁱ
- 26876602

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

cell surface receptor signaling pathway
Source: ProtInc
Traceable author statementⁱ
- 9029147
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest Source: Reactome
histone H4-R3 methylation
Source: UniProtKB
Inferred from direct assayⁱ
- 11448779
histone methylation
Source: UniProtKB
Inferred from direct assayⁱ
- 19405910
in utero embryonic development Source: Ensembl
negative regulation of megakaryocyte differentiation
Source: UniProtKB
Inferred from direct assayⁱ
- 20442406
neuron projection development
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 18773938
peptidyl-arginine methylation
Source: UniProtKB
Inferred from direct assayⁱ
positive regulation of cell proliferation
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 28040436
positive regulation of erythrocyte differentiation
Source: BHF-UCL
Inferred from mutant phenotypeⁱ
- 23483889
positive regulation of hemoglobin biosynthetic process Source: BHF-UCL
positive regulation of p38MAPK cascade Source: BHF-UCL
protein homooligomerization
Source: UniProtKB
Inferred from direct assayⁱ
- 26876602
protein methylation
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 26876602
- 28040436
regulation of megakaryocyte differentiation
Source: UniProtKB
Inferred from direct assayⁱ
- 26575292
regulation of transcription, DNA-templated Source: GO_Central

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Methyltransferase, Transferase
Ligand	S-adenosyl-L-methionine

Enzyme and pathway databases

BioCycⁱ	MetaCyc:HS05019-MONOMER
BRENDAⁱ	2.1.1.126 2681
Reactomeⁱ	R-HSA-3214858 RMTs methylate histone arginines R-HSA-6804114 TP53 Regulates Transcription of Genes Involved in G2 Cell Cycle Arrest R-HSA-8936459 RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function R-HSA-9018519 Estrogen-dependent gene expression
SABIO-RKⁱ	Q99873
SignaLinkⁱ	Q99873
SIGNORⁱ	Q99873

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Protein arginine N-methyltransferase 1Curated (EC:2.1.1.3195 Publications) Alternative name(s): Histone-arginine N-methyltransferase PRMT1 Interferon receptor 1-bound protein 4
Gene namesⁱ	Name:PRMT1Imported Synonyms:HMT2, HRMT1L22 Publications, IR1B4
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 19

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000126457.21
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:5187 PRMT1
MIMⁱ	602950 gene
neXtProtⁱ	NX_Q99873

Keywords - Cellular componentⁱ

Cytoplasm, Nucleus

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	92	V → A: Loss of FOXO1 methylation, its nuclear retention, and transcriptional activity. 1 Publication	1
Mutagenesisⁱ	93	L → A: Loss of FOXO1 methylation, its nuclear retention, and transcriptional activity. 1 Publication	1
Mutagenesisⁱ	94	D → A: Loss of FOXO1 methylation, its nuclear retention, and transcriptional activity. 1 Publication	1
Mutagenesisⁱ	280	Y → A: No effect on S-adenosyl-L-methionine binding but reduced EWS protein methylation; when associated with A-322 and A-359. No effect on homodimerization but loss of homooligomerization; when associated with A-322 and A-359. 1 Publication	1
Mutagenesisⁱ	322	Y → A: No effect on S-adenosyl-L-methionine binding but reduced EWS protein methylation; when associated with A-280 and A-359. No effect on homodimerization but loss of homooligomerization; when associated with A-280 and A-359. 1 Publication	1
Mutagenesisⁱ	359	L → A: No effect on S-adenosyl-L-methionine binding but reduced EWS protein methylation; when associated with A-280 and A-322. No effect on homodimerization but loss of homooligomerization; when associated with A-280 and A-322. 1 Publication	1

Organism-specific databases

DisGeNET More...DisGeNETⁱ	3276
Open Targets More...OpenTargetsⁱ	ENSG00000126457
PharmGKBⁱ	PA29461

Chemistry databases

ChEMBLⁱ	CHEMBL5524
Drug and drug target database More...DrugBankⁱ	DB01752 S-Adenosyl-L-Homocysteine
IUPHAR/BPS Guide to PHARMACOLOGY More...GuidetoPHARMACOLOGYⁱ	1252

Polymorphism and mutation databases

DMDMⁱ	161789011

DMDMⁱ

161789011

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000212321	1 – 371	Protein arginine N-methyltransferase 1Add BLAST		371

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	134	N6-succinyllysineBy similarity	1
Modified residueⁱ	304	PhosphoserineCombined sources	1
Modified residueⁱ	307	PhosphoserineCombined sources	1

Keywords - PTMⁱ

Phosphoprotein

Proteomic databases

EPDⁱ	Q99873
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q99873
PaxDbⁱ	Q99873
PeptideAtlas More...PeptideAtlasⁱ	Q99873
PRIDEⁱ	Q99873
ProteomicsDBⁱ	78511 78512 [Q99873-2] 78513 [Q99873-3]

PTM databases

iPTMnetⁱ	Q99873
PhosphoSitePlusⁱ	Q99873
SwissPalmⁱ	Q99873

Expressionⁱ

Tissue specificityⁱ

Widely expressed (PubMed:11097842). Expressed strongly in colorectal cancer cells (at protein level) (PubMed:28040436). Expressed strongly in colorectal cancer tissues compared to wild-type colon samples (at protein level) (PubMed:28040436). Expressed strongly in colorectal cancer tissues compared to wild-type colon samples (PubMed:28040436).2 Publications

Gene expression databases

Bgeeⁱ	ENSG00000126457 Expressed in 219 organ(s), highest expression level in embryo
CleanExⁱ	HS_PRMT1
ExpressionAtlasⁱ	Q99873 baseline and differential
Genevisibleⁱ	Q99873 HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	CAB022550 HPA069769 HPA072136

HPAⁱ

CAB022550
HPA069769
HPA072136

Interactionⁱ

Subunit structureⁱ

Homodimer (PubMed:16879614, PubMed:26876602). Homooctamer; individual homodimers associates to form a homooctamer (PubMed:26876602). Individual homodimers can associate to form a homohexamer. Heterodimer with PRMT8. Interacts with BTG1, BTG2, NFATC2IP and IFNAR1 (By similarity). Interacts with and methylates CHTOP, thereby enabling the interaction of CHTOP with the 5FMC complex (PubMed:25284789). Interacts with ILF3 and SUPT5H. Interacts with and methylates FOXO1, leading to the nuclear retention of FOXO1 and the stimulation of FOXO1 transcriptional activity. Methylation of FOXO1 is increased upon oxidative stress. Interacts with and probably methylates ATXN2L (PubMed:25748791). Component of the methylosome, a 20S complex containing at least CLNS1A/pICln, PRMT5/SKB1, WDR77/MEP50, PRMT1 and ERH (PubMed:25284789). Interacts with DHX9 (via RGG region) (PubMed:15084609). Interacts (via N-terminus) with HABP4 (PubMed:16879614).By similarity10 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
itself		4	EBI-78738,EBI-78738
CHTOP	Q9Y3Y2	2	EBI-78738,EBI-347794
DCAF8	Q5TAQ9	2	EBI-78738,EBI-740686
DHX9	Q08211	2	EBI-78738,EBI-352022
EWSR1	Q01844	2	EBI-78738,EBI-739737
FAM9A	Q8IZU1	2	EBI-78738,EBI-8468186
gag	P04591	2	EBI-78738,EBI-6179727	From Human immunodeficiency virus type 1 group M subtype B (isolate HXB2).
HABP4	Q5JVS0	2	EBI-78738,EBI-523625
HIST2H4B	P62805	2	EBI-78738,EBI-302023
HNRNPK	P61978	3	EBI-78738,EBI-304185
HNRNPR	O43390	4	EBI-78738,EBI-713419
ILF3	Q12906	2	EBI-78738,EBI-78756
NR4A1	P22736	6	EBI-78738,EBI-721550
NRIP1	P48552	4	EBI-78738,EBI-746484
OFCC1	Q8IZS5	2	EBI-78738,EBI-8477661
PRMT8	Q9NR22	4	EBI-17165527,EBI-745545
PRMT8	Q9NR22-2	5	EBI-78738,EBI-10186886
SCN5A	Q14524	2	EBI-78738,EBI-726858
SPEG	Q15772	3	EBI-78738,EBI-1384196
SPSB1	Q96BD6	2	EBI-78738,EBI-2659201
SPSB2	Q99619	2	EBI-78738,EBI-2323209
SYNCRIP	O60506	2	EBI-78738,EBI-1024357
VHL	P40337	2	EBI-78738,EBI-301246
WDYHV1	Q96HA8	3	EBI-78738,EBI-741158

GO - Molecular functionⁱ

enzyme binding
Source: UniProtKB
Inferred from physical interactionⁱ
- 19460357
identical protein binding
Source: UniProtKB
Inferred from direct assayⁱ
- 26876602
mitogen-activated protein kinase p38 binding
Source: BHF-UCL
Inferred from physical interactionⁱ
- 23483889

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	109512, 218 interactors
CORUMⁱ	Q99873
Database of interacting proteins More...DIPⁱ	DIP-30878N
IntActⁱ	Q99873, 98 interactors
Molecular INTeraction database More...MINTⁱ	Q99873
STRINGⁱ	9606.ENSP00000406162

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q99873
SMRⁱ	Q99873
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	50 – 361	SAM-dependent MTase PRMT-typePROSITE-ProRule annotationAdd BLAST		312

Sequence similaritiesⁱ

Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N-methyltransferase family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGⁱ	KOG1499 Eukaryota ENOG410XQYH LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00550000074406
HOGENOMⁱ	HOG000198521
HOVERGENⁱ	HBG001793
InParanoidⁱ	Q99873
KEGG Orthology (KO) More...KOⁱ	K11434
OMAⁱ	RCHKRIG
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0ADC
PhylomeDBⁱ	Q99873
TreeFamⁱ	TF300608

Family and domain databases

InterProⁱ	View protein in InterPro IPR025799 Arg_MeTrfase IPR029063 SAM-dependent_MTases
SUPFAMⁱ	SSF53335 SSF53335, 1 hit
PROSITEⁱ	View protein in PROSITE PS51678 SAM_MT_PRMT, 1 hit

Sequences (4+)ⁱ

Sequence statusⁱ: Complete.

This entry describes 4 isoformsⁱ produced by alternative splicing. Align Add to basket

This entry has 4 described isoforms and 8 potential isoforms that are computationally mapped.Show all Align All

Isoform 1 (identifier: Q99873-1) [UniParc]FASTA Add to basket

Also known as: V2

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAAEAANCI MENFVATLAN GMSLQPPLEE VSCGQAESSE KPNAEDMTSK 
        60         70         80         90        100
DYYFDSYAHF GIHEEMLKDE VRTLTYRNSM FHNRHLFKDK VVLDVGSGTG 
       110        120        130        140        150
ILCMFAAKAG ARKVIGIECS SISDYAVKIV KANKLDHVVT IIKGKVEEVE 
       160        170        180        190        200
LPVEKVDIII SEWMGYCLFY ESMLNTVLYA RDKWLAPDGL IFPDRATLYV 
       210        220        230        240        250
TAIEDRQYKD YKIHWWENVY GFDMSCIKDV AIKEPLVDVV DPKQLVTNAC 
       260        270        280        290        300
LIKEVDIYTV KVEDLTFTSP FCLQVKRNDY VHALVAYFNI EFTRCHKRTG 
       310        320        330        340        350
FSTSPESPYT HWKQTVFYME DYLTVKTGEE IFGTIGMRPN AKNNRDLDFT 
       360        370 
IDLDFKGQLC ELSCSTDYRM R

Length:371

Mass (Da):42,462

Last modified:March 28, 2018 - v3

Checksum:ⁱ544349801B0E1396

GO

Isoform 2 (identifier: Q99873-2) [UniParc]FASTA Add to basket

Also known as: V3

The sequence of this isoform differs from the canonical sequence as follows:
1-29: MAAAEAANCIMENFVATLANGMSLQPPLE → MVGVA

« Hide

        10         20         30         40         50
MVGVAEVSCG QAESSEKPNA EDMTSKDYYF DSYAHFGIHE EMLKDEVRTL 
        60         70         80         90        100
TYRNSMFHNR HLFKDKVVLD VGSGTGILCM FAAKAGARKV IGIECSSISD 
       110        120        130        140        150
YAVKIVKANK LDHVVTIIKG KVEEVELPVE KVDIIISEWM GYCLFYESML 
       160        170        180        190        200
NTVLYARDKW LAPDGLIFPD RATLYVTAIE DRQYKDYKIH WWENVYGFDM 
       210        220        230        240        250
SCIKDVAIKE PLVDVVDPKQ LVTNACLIKE VDIYTVKVED LTFTSPFCLQ 
       260        270        280        290        300
VKRNDYVHAL VAYFNIEFTR CHKRTGFSTS PESPYTHWKQ TVFYMEDYLT 
       310        320        330        340 
VKTGEEIFGT IGMRPNAKNN RDLDFTIDLD FKGQLCELSC STDYRMR

Show »

Length:347

Mass (Da):39,929

Checksum:ⁱ2546A25EF744E265

GO

Isoform 3 (identifier: Q99873-3) [UniParc]FASTA Add to basket

Also known as: V1

The sequence of this isoform differs from the canonical sequence as follows:
11-30: MENFVATLANGMSLQPPLEE → ME

« Hide

        10         20         30         40         50
MAAAEAANCI MEVSCGQAES SEKPNAEDMT SKDYYFDSYA HFGIHEEMLK 
        60         70         80         90        100
DEVRTLTYRN SMFHNRHLFK DKVVLDVGSG TGILCMFAAK AGARKVIGIE 
       110        120        130        140        150
CSSISDYAVK IVKANKLDHV VTIIKGKVEE VELPVEKVDI IISEWMGYCL 
       160        170        180        190        200
FYESMLNTVL YARDKWLAPD GLIFPDRATL YVTAIEDRQY KDYKIHWWEN 
       210        220        230        240        250
VYGFDMSCIK DVAIKEPLVD VVDPKQLVTN ACLIKEVDIY TVKVEDLTFT 
       260        270        280        290        300
SPFCLQVKRN DYVHALVAYF NIEFTRCHKR TGFSTSPESP YTHWKQTVFY 
       310        320        330        340        350
MEDYLTVKTG EEIFGTIGMR PNAKNNRDLD FTIDLDFKGQ LCELSCSTDY 

RMR

Show »

Length:353

Mass (Da):40,548

Checksum:ⁱ1704BA3762F3E264

GO

Isoform 4 (identifier: Q99873-5) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
13-30: Missing.
186-253: Missing.

« Hide

        10         20         30         40         50
MAAAEAANCI MEVSCGQAES SEKPNAEDMT SKDYYFDSYA HFGIHEEMLK 
        60         70         80         90        100
DEVRTLTYRN SMFHNRHLFK DKVVLDVGSG TGILCMFAAK AGARKVIGIE 
       110        120        130        140        150
CSSISDYAVK IVKANKLDHV VTIIKGKVEE VELPVEKVDI IISEWMGYCL 
       160        170        180        190        200
FYESMLNTVL YARDKWLEVD IYTVKVEDLT FTSPFCLQVK RNDYVHALVA 
       210        220        230        240        250
YFNIEFTRCH KRTGFSTSPE SPYTHWKQTV FYMEDYLTVK TGEEIFGTIG 
       260        270        280 
MRPNAKNNRD LDFTIDLDFK GQLCELSCST DYRMR

Show »

Length:285

Mass (Da):32,693

Checksum:ⁱ7D985F187D73F7E3

GO

Computationally mapped potential isoform sequencesⁱ

There are 8 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

H0YDE4	H0YDE4_HUMAN	Protein arginine N-methyltransferas... Protein arginine N-methyltransferase 1	PRMT1	238	Annotation score:
E9PKG1	E9PKG1_HUMAN	Protein arginine N-methyltransferas... Protein arginine N-methyltransferase 1	PRMT1	325	Annotation score:
E9PMW9	E9PMW9_HUMAN	Protein arginine N-methyltransferas... Protein arginine N-methyltransferase 1	PRMT1	110	Annotation score:
E9PQ98	E9PQ98_HUMAN	Protein arginine N-methyltransferas... Protein arginine N-methyltransferase 1	PRMT1	205	Annotation score:
E9PNR9	E9PNR9_HUMAN	Protein arginine N-methyltransferas... Protein arginine N-methyltransferase 1	PRMT1	160	Annotation score:
E9PIX6	E9PIX6_HUMAN	Protein arginine N-methyltransferas... Protein arginine N-methyltransferase 1	PRMT1	207	Annotation score:
E9PMZ2	E9PMZ2_HUMAN	Protein arginine N-methyltransferas... Protein arginine N-methyltransferase 1	PRMT1	63	Annotation score:
E9PIE2	E9PIE2_HUMAN	Protein arginine N-methyltransferas... Protein arginine N-methyltransferase 1	PRMT1	54	Annotation score:

Sequence cautionⁱ

The sequence AAF62894 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

The sequence AAF62895 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

The sequence AAI09283 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

The sequence AAI09284 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

The sequence BAA11029 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

The sequence CAA71764 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

The sequence CAA71765 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	118	E → V in CAA71763 (PubMed:9545638).Curated	1
Sequence conflictⁱ	118	E → V in CAA71764 (PubMed:9545638).Curated	1
Sequence conflictⁱ	118	E → V in CAA71765 (PubMed:9545638).Curated	1
Sequence conflictⁱ	118	E → V in BAA11029 (PubMed:8675017).Curated	1
Sequence conflictⁱ	157 – 185	DIIIS…RDKWL → ASSSASGWATASSTSPCSTP CSMPGTSV in BAA11029 (PubMed:8675017).CuratedAdd BLAST	29
Sequence conflictⁱ	212	K → E in BAG65435 (PubMed:14702039).Curated	1

Natural variant

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Natural variantⁱVAR_037501	88	K → M. Corresponds to variant dbSNP:rs1804486Ensembl.		1
Natural variantⁱVAR_037502	168	L → F. Corresponds to variant dbSNP:rs11673683Ensembl.		1

Alternative sequence

Feature key	Position(s)	DescriptionActions	Length
Alternative sequenceⁱVSP_005208	1 – 29	MAAAE…QPPLE → MVGVA in isoform 2. 1 PublicationAdd BLAST	29
Alternative sequenceⁱVSP_005209	11 – 30	MENFV…PPLEE → ME in isoform 3. 2 PublicationsAdd BLAST	20
Alternative sequenceⁱVSP_059419	13 – 30	Missing in isoform 4. Add BLAST	18
Alternative sequenceⁱVSP_059420	186 – 253	Missing in isoform 4. Add BLAST	68

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	Y10805 mRNA Translation: CAA71763.1 Y10806 mRNA Translation: CAA71764.1 Different initiation. Y10807 mRNA Translation: CAA71765.1 Different initiation. D66904 mRNA Translation: BAA11029.1 Different initiation. AF222689 Genomic DNA Translation: AAF62893.1 AF222689 Genomic DNA Translation: AAF62894.1 Different initiation. AF222689 Genomic DNA Translation: AAF62895.1 Different initiation. AK304660 mRNA Translation: BAG65435.1 CR407608 mRNA Translation: CAG28536.1 AC011495 Genomic DNA No translation available. CH471177 Genomic DNA Translation: EAW52521.1 CH471177 Genomic DNA Translation: EAW52519.1 BC019268 mRNA Translation: AAH19268.2 BC109282 mRNA Translation: AAI09283.2 Different initiation. BC109283 mRNA Translation: AAI09284.2 Different initiation.
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS42592.1 [Q99873-3] CCDS46145.1 [Q99873-1] CCDS74425.1 [Q99873-5]
NCBI Reference Sequences More...RefSeqⁱ	NP_001193971.1, NM_001207042.2 [Q99873-5] NP_001527.3, NM_001536.5 [Q99873-1] NP_938074.2, NM_198318.4 [Q99873-3] XP_016882223.1, XM_017026734.1 [Q99873-2] XP_016882224.1, XM_017026735.1 [Q99873-2]
UniGeneⁱ	Hs.20521

Genome annotation databases

Ensemblⁱ	ENST00000391851; ENSP00000375724; ENSG00000126457 [Q99873-3] ENST00000454376; ENSP00000406162; ENSG00000126457 [Q99873-1] ENST00000610806; ENSP00000484505; ENSG00000126457 [Q99873-5]
GeneIDⁱ	3276
KEGGⁱ	hsa:3276
UCSC genome browser More...UCSCⁱ	uc002ppe.4 human [Q99873-1] uc010enf.3 human

Keywords - Coding sequence diversityⁱ

Alternative splicing, Polymorphism

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q99873	PRMT1 isoform 11		PANTR		371	UniRef100_Q99873
Uncharacterized protein		MACMU		371
protein arginine N-methyltransferase 1 isoform X1		TARSY		371
PRMT1 isoform 11		PONAB		371
Uncharacterized protein		CERAT		371
+17
Q99873-2	Uncharacterized protein		ICTTR		347	UniRef100_A0A287D936
Uncharacterized protein		PROCO		347
protein arginine N-methyltransferase 1 isoform X4		Enhydra lutris kenyoni		347
Uncharacterized protein		DESRO		347
protein arginine N-methyltransferase 1 isoform X3		DIPOR		347
Q99873-3	Protein arginine N-methyltransferase 1 isoform 3		MACMU		353	UniRef100_I2CUI2
PRMT1 isoform 6		PANTR		353
HMT1 hnRNP methyltransferase-like 2 isoform 3		BOVIN		353
Macaca fascicularis brain cDNA clone: QbsB-10377, similar to human HMT1 hnRNP methyltransferase-like 2 (S. cerevisiae)(HRMT1L2), transcript variant 3, mRNA, RefSeq: NM_198318.1		MACFA		353
protein arginine N-methyltransferase 1 isoform X2		TARSY		353
+17
Q99873-5	PRMT1 isoform 7		PANTR		285	UniRef100_K7D7S7
Uncharacterized protein		MACMU		285
protein arginine N-methyltransferase 1 isoform X5		Enhydra lutris kenyoni		285
PRMT1 isoform 7		PONAB		285
Uncharacterized protein		CERAT		285
+9

Protein	Similar proteins		Species	Score	Length	Source
Q99873	Protein arginine N-methyltransferase 1		MOUSE		371	UniRef90_Q99873
Protein arginine N-methyltransferase 1-A		XENLA		369
PRMT1 isoform 11		PANTR		371
Uncharacterized protein		MACMU		371
protein arginine N-methyltransferase 1 isoform X1		TARSY		371
+142
Q99873-2	Protein arginine N-methyltransferase 1	)	RAT		353	UniRef90_Q63009
Protein arginine N-methyltransferase 1		XENTR		351
Uncharacterized protein		NEOLE		353
Uncharacterized protein		MOUSE		353
Protein arginine methyltransferase 1		FELCA		355
+152
Q99873-3	Protein arginine N-methyltransferase 1	)	RAT		353	UniRef90_Q63009
Protein arginine N-methyltransferase 1		XENTR		351
Uncharacterized protein		NEOLE		353
Uncharacterized protein		MOUSE		353
Protein arginine methyltransferase 1		FELCA		355
+152
Q99873-5	Protein arginine N-methyltransferase 1 (Fragment)		MOUSE		217	UniRef90_A0A140LHS4
Protein arginine methyltransferase 1		SAIBB		291
PRMT1 isoform 7		PANTR		285
Uncharacterized protein		MACMU		285
protein arginine N-methyltransferase 1 isoform X5		Enhydra lutris kenyoni		285
+30

Protein	Similar proteins		Species	Score	Length	Source
Q99873	Protein arginine N-methyltransferase 1		MOUSE		371	UniRef50_Q99873
Protein arginine N-methyltransferase 1-A		XENLA		369
Protein arginine N-methyltransferase 1.1		ARATH		390
Probable protein arginine N-methyltransferase 1		ORYSI		387
Probable protein arginine N-methyltransferase 1		ORYSJ		387
+615
Q99873-2	Protein arginine N-methyltransferase 1	)	RAT		353	UniRef50_Q63009
Protein arginine N-methyltransferase 1		XENTR		351
Uncharacterized protein		NEOLE		353
Uncharacterized protein		MOUSE		353
Protein arginine methyltransferase 1		FELCA		355
+514
Q99873-3	Protein arginine N-methyltransferase 1	)	RAT		353	UniRef50_Q63009
Protein arginine N-methyltransferase 1		XENTR		351
Uncharacterized protein		NEOLE		353
Uncharacterized protein		MOUSE		353
Protein arginine methyltransferase 1		FELCA		355
+514
Q99873-5	Uncharacterized protein		BOVIN		336	UniRef50_F1MP07
Uncharacterized protein		PANPA		325
Uncharacterized protein		PANTR		325
Uncharacterized protein		GORGO		323
Uncharacterized protein		NOMLE		301
+54

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	Y10805 mRNA Translation: CAA71763.1 Y10806 mRNA Translation: CAA71764.1 Different initiation. Y10807 mRNA Translation: CAA71765.1 Different initiation. D66904 mRNA Translation: BAA11029.1 Different initiation. AF222689 Genomic DNA Translation: AAF62893.1 AF222689 Genomic DNA Translation: AAF62894.1 Different initiation. AF222689 Genomic DNA Translation: AAF62895.1 Different initiation. AK304660 mRNA Translation: BAG65435.1 CR407608 mRNA Translation: CAG28536.1 AC011495 Genomic DNA No translation available. CH471177 Genomic DNA Translation: EAW52521.1 CH471177 Genomic DNA Translation: EAW52519.1 BC019268 mRNA Translation: AAH19268.2 BC109282 mRNA Translation: AAI09283.2 Different initiation. BC109283 mRNA Translation: AAI09284.2 Different initiation.
CCDSⁱ	CCDS42592.1 [Q99873-3] CCDS46145.1 [Q99873-1] CCDS74425.1 [Q99873-5]
RefSeqⁱ	NP_001193971.1, NM_001207042.2 [Q99873-5] NP_001527.3, NM_001536.5 [Q99873-1] NP_938074.2, NM_198318.4 [Q99873-3] XP_016882223.1, XM_017026734.1 [Q99873-2] XP_016882224.1, XM_017026735.1 [Q99873-2]
UniGeneⁱ	Hs.20521

3D structure databases

ProteinModelPortalⁱ	Q99873
SMRⁱ	Q99873
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	109512, 218 interactors
CORUMⁱ	Q99873
DIPⁱ	DIP-30878N
IntActⁱ	Q99873, 98 interactors
MINTⁱ	Q99873
STRINGⁱ	9606.ENSP00000406162

Chemistry databases

ChEMBLⁱ	CHEMBL5524
DrugBankⁱ	DB01752 S-Adenosyl-L-Homocysteine
GuidetoPHARMACOLOGYⁱ	1252

PTM databases

iPTMnetⁱ	Q99873
PhosphoSitePlusⁱ	Q99873
SwissPalmⁱ	Q99873

Polymorphism and mutation databases

DMDMⁱ	161789011

DMDMⁱ

161789011

Proteomic databases

EPDⁱ	Q99873
MaxQBⁱ	Q99873
PaxDbⁱ	Q99873
PeptideAtlasⁱ	Q99873
PRIDEⁱ	Q99873
ProteomicsDBⁱ	78511 78512 [Q99873-2] 78513 [Q99873-3]

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	3276
Structural Biology Knowledgebase	Search...

Genome annotation databases

Ensemblⁱ	ENST00000391851; ENSP00000375724; ENSG00000126457 [Q99873-3] ENST00000454376; ENSP00000406162; ENSG00000126457 [Q99873-1] ENST00000610806; ENSP00000484505; ENSG00000126457 [Q99873-5]
GeneIDⁱ	3276
KEGGⁱ	hsa:3276
UCSCⁱ	uc002ppe.4 human [Q99873-1] uc010enf.3 human

Organism-specific databases

CTDⁱ	3276
DisGeNETⁱ	3276
EuPathDBⁱ	HostDB:ENSG00000126457.21
GeneCardsⁱ	PRMT1
HGNCⁱ	HGNC:5187 PRMT1
HPAⁱ	CAB022550 HPA069769 HPA072136
MIMⁱ	602950 gene
neXtProtⁱ	NX_Q99873
OpenTargetsⁱ	ENSG00000126457
PharmGKBⁱ	PA29461
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	KOG1499 Eukaryota ENOG410XQYH LUCA
GeneTreeⁱ	ENSGT00550000074406
HOGENOMⁱ	HOG000198521
HOVERGENⁱ	HBG001793
InParanoidⁱ	Q99873
KOⁱ	K11434
OMAⁱ	RCHKRIG
OrthoDBⁱ	EOG091G0ADC
PhylomeDBⁱ	Q99873
TreeFamⁱ	TF300608

Enzyme and pathway databases

BioCycⁱ	MetaCyc:HS05019-MONOMER
BRENDAⁱ	2.1.1.126 2681
Reactomeⁱ	R-HSA-3214858 RMTs methylate histone arginines R-HSA-6804114 TP53 Regulates Transcription of Genes Involved in G2 Cell Cycle Arrest R-HSA-8936459 RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function R-HSA-9018519 Estrogen-dependent gene expression
SABIO-RKⁱ	Q99873
SignaLinkⁱ	Q99873
SIGNORⁱ	Q99873

Miscellaneous databases

ChiTaRSⁱ	PRMT1 human
GeneWikiⁱ	PRMT1
GenomeRNAiⁱ	3276
Protein Ontology More...PROⁱ	PR:Q99873
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000126457 Expressed in 219 organ(s), highest expression level in embryo
CleanExⁱ	HS_PRMT1
ExpressionAtlasⁱ	Q99873 baseline and differential
Genevisibleⁱ	Q99873 HS

Family and domain databases

InterProⁱ	View protein in InterPro IPR025799 Arg_MeTrfase IPR029063 SAM-dependent_MTases
SUPFAMⁱ	SSF53335 SSF53335, 1 hit
PROSITEⁱ	View protein in PROSITE PS51678 SAM_MT_PRMT, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	ANM1_HUMAN
Accessionⁱ	Q99873Primary (citable) accession number: Q99873 Secondary accession number(s): A0A087X1W2 Q9NZ06
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
	Last sequence update:	March 28, 2018
	Last modified:	October 10, 2018
	This is version 170 of the entry and version 3 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations
SIMILARITY comments
Index of protein domains and families
Human chromosome 19
Human chromosome 19: entries, gene names and cross-references to MIM
Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

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UniProtKB - Q99873 (ANM1_HUMAN)

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Protein arginine N-methyltransferase 1

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

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<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

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Functionⁱ

Kineticsⁱ

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Keywords - Cellular componentⁱ

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PTM / Processingⁱ

Keywords - PTMⁱ

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Structureⁱ

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Keywords - Coding sequence diversityⁱ

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Keywords - Technical termⁱ