TSG101

Functionⁱ

Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses. Required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413). It may also play a role in the extracellular release of microvesicles that differ from the exosomes (PubMed:22315426).7 Publications

GO - Molecular functionⁱ

calcium-dependent protein binding
Source: UniProtKB
Inferred from physical interactionⁱ
- 19520058
DNA binding
Source: ProtInc
Traceable author statementⁱ
- 10888872
nuclear receptor transcription coactivator activity Source: Ensembl
protein-containing complex binding
Source: UniProtKB
Inferred from direct assayⁱ
- 16973552
protein homodimerization activity
Source: UniProtKB
Inferred from physical interactionⁱ
- 14519844
transcription corepressor activity
Source: ProtInc
Traceable author statementⁱ
- 10888872
ubiquitin binding
Source: UniProtKB
Inferred from direct assayⁱ
ubiquitin protein ligase binding
Source: UniProtKB
Inferred from physical interactionⁱ
- 17229889
virion binding
Source: UniProtKB
Inferred from direct assayⁱ
- 11595185

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

autophagosome maturation
Source: ParkinsonsUK-UCL
Traceable author statementⁱ
- 21118109
cell cycle arrest Source: Ensembl
cell division Source: UniProtKB-KW
endosomal transport Source: Reactome
endosome to lysosome transport Source: Ensembl
exosomal secretion Source: Ensembl
extracellular transport
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 22315426
keratinocyte differentiation Source: Ensembl
macroautophagy
Source: ParkinsonsUK-UCL
Traceable author statementⁱ
- 20588296
multivesicular body assembly
Source: ParkinsonsUK-UCL
Traceable author statementⁱ
- 20588296
negative regulation of cell proliferation Source: Ensembl
negative regulation of epidermal growth factor-activated receptor activity
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 16973552
negative regulation of epidermal growth factor receptor signaling pathway
Source: ParkinsonsUK-UCL
Inferred from mutant phenotypeⁱ
- 16554368
negative regulation of transcription, DNA-templated Source: Ensembl
positive regulation of exosomal secretion
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 22660413
- 24105262
positive regulation of ubiquitin-dependent endocytosis Source: Ensembl
positive regulation of viral budding via host ESCRT complex
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 11595185
- 15218037
positive regulation of viral process
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 18005716
positive regulation of viral release from host cell
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 11595185
- 15218037
protein monoubiquitination Source: Ensembl
protein transport Source: UniProtKB-KW
regulation of cell growth Source: Ensembl
regulation of extracellular exosome assembly
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 24105262
regulation of MAP kinase activity
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 17714434
regulation of viral budding via host ESCRT complex
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 18005716
ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway
Source: HGNC
Inferred from direct assayⁱ
- 15611048
viral budding
Source: BHF-UCL
Inferred from mutant phenotypeⁱ
- 15126635
viral budding via host ESCRT complex
Source: ParkinsonsUK-UCL
Traceable author statementⁱ
- 20588296
viral life cycle Source: Reactome

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Biological process	Cell cycle, Cell division, Growth regulation, Host-virus interaction, Protein transport, Transport

Biological process

Cell cycle, Cell division, Growth regulation, Host-virus interaction, Protein transport, Transport

Enzyme and pathway databases

Reactomeⁱ	R-HSA-162588 Budding and maturation of HIV virion R-HSA-174490 Membrane binding and targetting of GAG proteins R-HSA-917729 Endosomal Sorting Complex Required For Transport (ESCRT)

Reactomeⁱ

R-HSA-162588 Budding and maturation of HIV virion
R-HSA-174490 Membrane binding and targetting of GAG proteins
R-HSA-917729 Endosomal Sorting Complex Required For Transport (ESCRT)

Protein family/group databases

MoonDBⁱ	Q99816 Curated

MoonDBⁱ

Q99816 Curated

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Tumor susceptibility gene 101 protein Alternative name(s): ESCRT-I complex subunit TSG101
Gene namesⁱ	Name:TSG101
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 11

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000074319.12
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:15971 TSG101
MIMⁱ	601387 gene
neXtProtⁱ	NX_Q99816

Keywords - Cellular componentⁱ

Cytoplasm, Cytoskeleton, Endosome, Membrane, Nucleus

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	43	V → A: Reduces interaction with ubiquitin; inhibits down-regulation of EGFR. 1 Publication	1
Mutagenesisⁱ	45	N → A: Reduces interaction with ubiquitin. No effect on MGRN1-binding. 3 Publications	1
Mutagenesisⁱ	46	D → A: Reduces interaction with ubiquitin. 1 Publication	1
Mutagenesisⁱ	63	Y → A: Reduces interaction with HIV-1 p6; impairs HIV-1 budding. 1 Publication	1
Mutagenesisⁱ	88	F → A: Reduces interaction with ubiquitin; no effect on in interaction with HIV-1 p6. 1 Publication	1
Mutagenesisⁱ	89	V → A: No change in interaction with p6; no effect on HIV-1 budding. 1 Publication	1
Mutagenesisⁱ	95	M → A: Reduces interaction with VPS37B and HIV-1 p6; abolishes interaction with PDCD6IP; impairs HIV-1 budding; inhibits down-regulation of EGFR. Abolishes MGRN1-binding. Loss of interaction with ARRDC1. 6 Publications	1
Mutagenesisⁱ	141	V → A: Reduces interaction with HIV-1 p6. 1 Publication	1
Mutagenesisⁱ	158 – 162	Missing : Abolishes interaction with CEP55 and midbody localization; no effect on interaction with ESCRT-I proteins, PDCD6IP and viral proteins. 1 Publication	5
Mutagenesisⁱ	158 – 160	PPN → AAA: Abolishes interaction with CEP55. 1 Publication	3
Mutagenesisⁱ	368 – 371	RKQF → AAAA: Loss of interaction with VPS28. No effect on interaction with VPS37C. 1 Publication	4

Organism-specific databases

DisGeNET More...DisGeNETⁱ	7251
MalaCards human disease database More...MalaCardsⁱ	TSG101
Open Targets More...OpenTargetsⁱ	ENSG00000074319
PharmGKBⁱ	PA38068

Chemistry databases

ChEMBLⁱ	CHEMBL6157

ChEMBLⁱ

CHEMBL6157

Polymorphism and mutation databases

BioMutaⁱ	TSG101
DMDMⁱ	9789790

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Initiator methionineⁱ		RemovedCombined sources
ChainⁱPRO_0000082606	2 – 390	Tumor susceptibility gene 101 proteinAdd BLAST		389

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Modified residueⁱ	2	N-acetylalanineCombined sources		1
Modified residueⁱ	220	PhosphothreonineCombined sources		1

Post-translational modificationⁱ

Monoubiquitinated at multiple sites by LRSAM1 and by MGRN1. Ubiquitination inactivates it, possibly by regulating its shuttling between an active membrane-bound protein and an inactive soluble form. Ubiquitination by MGRN1 requires the presence of UBE2D1.3 Publications

Keywords - PTMⁱ

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDⁱ	Q99816
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q99816
PaxDbⁱ	Q99816
PeptideAtlas More...PeptideAtlasⁱ	Q99816
PRIDEⁱ	Q99816
ProteomicsDBⁱ	78490 78491 [Q99816-2]

PTM databases

iPTMnetⁱ	Q99816
PhosphoSitePlusⁱ	Q99816

Expressionⁱ

Tissue specificityⁱ

Heart, brain, placenta, lung, liver, skeletal, kidney and pancreas.

Gene expression databases

Bgeeⁱ	ENSG00000074319 Expressed in 229 organ(s), highest expression level in oocyte
CleanExⁱ	HS_TSG101
ExpressionAtlasⁱ	Q99816 baseline and differential
Genevisibleⁱ	Q99816 HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	CAB004283 HPA006161

HPAⁱ

CAB004283
HPA006161

Interactionⁱ

Subunit structureⁱ

Component of the ESCRT-I complex (endosomal sorting complex required for transport I) which consists of TSG101, VPS28, a VPS37 protein (VPS37A to -D) and MVB12A or MVB12B in a 1:1:1:1 stoichiometry (PubMed:18005716). Interacts with VPS37A, VPS37B and VPS37C (PubMed:15218037, PubMed:15509564). Interacts with DMAP1 (PubMed:10888872). Interacts with ubiquitin (PubMed:11595185). Interacts with stathmin, GMCL and AATF (By similarity). Component of an ESCRT-I complex (endosomal sorting complex required for transport I) which consists of TSG101, VPS28, VPS37A and UBAP1 in a 1:1:1:1 stoichiometry (PubMed:21757351). Interacts with HGS; the interaction mediates the association with the ESCRT-0 complex. Interacts with GGA1 and GGA3 (PubMed:15143060, PubMed:15039775). Interacts (via UEV domain) with PDCD6IP/AIP1 (PubMed:14505570, PubMed:14519844). Interacts with VPS28, SNF8 and VPS36 (PubMed:14505570). Self-associates (PubMed:14505570, PubMed:14519844). Interacts with MVB12A; the association appears to be mediated by the TSG101-VPS37 binary subcomplex. Interacts with VPS37D. Interacts with LRSAM1. Interacts with CEP55; the interaction is required for cytokinesis but not for viral budding (PubMed:17853893). Interacts with PDCD6 (PubMed:18256029). Interacts with LITAF (PubMed:23166352). Interacts with MGRN1 (PubMed:17229889). Interacts with ARRDC1; recruits TSG101 to the plasma membrane (PubMed:21191027, PubMed:22315426).By similarity19 Publications

(Microbial infection) Interacts with HIV-1 p6.5 Publications

(Microbial infection) Interacts with human spumavirus Gag.1 Publication

(Microbial infection) Interacts with HTLV-1 Gag.1 Publication

(Microbial infection) Interacts with Ebola virus VP40.1 Publication

(Microbial infection) Interacts with EIAV p9; the interaction has been shown in vitro.1 Publication

(Microbial infection) Interacts with Lassa virus protein Z.1 Publication

(Microbial infection) Interacts with hepatitis E virus protein ORF3.1 Publication

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
BAG4	O95429	4	EBI-346882,EBI-2949658
CD2AP	Q9Y5K6	2	EBI-346882,EBI-298152
CEP55	Q53EZ4	23	EBI-346882,EBI-747776
DAXX	Q9UER7	4	EBI-346882,EBI-77321
FAM9B	Q8IZU0	3	EBI-346882,EBI-10175124
gag	P04591	3	EBI-15891993,EBI-10634977	From Human immunodeficiency virus type 1 group M subtype B (isolate HXB2).
HAUS1	Q96CS2	5	EBI-346882,EBI-2514791
HGS	O14964	4	EBI-346882,EBI-740220
Hgs	Q99LI8	3	EBI-346882,EBI-2119135	From Mus musculus.
IQGAP1	P46940	5	EBI-346882,EBI-297509
KIFC3	Q9BVG8	3	EBI-346882,EBI-2125614
KRT13	A1A4E9	3	EBI-346882,EBI-10171552
KRT18	P05783	3	EBI-346882,EBI-297888
KRT31	Q15323	5	EBI-346882,EBI-948001
LITAF	Q99732	3	EBI-346882,EBI-725647
LRSAM1	Q6UWE0	9	EBI-346882,EBI-720984
MGRN1	O60291	4	EBI-346882,EBI-2129851
PDLIM7	Q9NR12	5	EBI-346882,EBI-350517
PTPN23	Q9H3S7	2	EBI-346882,EBI-724478
ROCK1	Q13464	4	EBI-346882,EBI-876651
SYCE1	Q8N0S2	5	EBI-346882,EBI-6872807
TAX1BP1	Q86VP1	5	EBI-346882,EBI-529518
USHBP1	Q8N6Y0	3	EBI-346882,EBI-739895
VPS28	Q9UK41	9	EBI-346882,EBI-727424
VPS37A	Q8NEZ2	5	EBI-346882,EBI-2850578
VPS37A	Q8NEZ2-2	5	EBI-346882,EBI-10270911
VPS37C	A5D8V6	7	EBI-346882,EBI-2559305

GO - Molecular functionⁱ

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	113102, 175 interactors
CORUMⁱ	Q99816
Database of interacting proteins More...DIPⁱ	DIP-31809N
ELMⁱ	Q99816
IntActⁱ	Q99816, 135 interactors
Molecular INTeraction database More...MINTⁱ	Q99816
STRINGⁱ	9606.ENSP00000251968

Chemistry databases

BindingDBⁱ

Q99816

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Helixⁱ	5 – 11	Combined sources	7
Turnⁱ	12 – 14	Combined sources	3
Helixⁱ	18 – 31	Combined sources	14
Beta strandⁱ	35 – 42	Combined sources	8
Turnⁱ	47 – 49	Combined sources	3
Beta strandⁱ	51 – 62	Combined sources	12
Beta strandⁱ	67 – 75	Combined sources	9
Turnⁱ	78 – 82	Combined sources	5
Beta strandⁱ	86 – 89	Combined sources	4
Beta strandⁱ	95 – 97	Combined sources	3
Beta strandⁱ	100 – 103	Combined sources	4
Beta strandⁱ	107 – 109	Combined sources	3
Helixⁱ	112 – 115	Combined sources	4
Turnⁱ	119 – 121	Combined sources	3
Helixⁱ	124 – 137	Combined sources	14
Beta strandⁱ	140 – 143	Combined sources	4
Helixⁱ	230 – 299	Combined sources	70

Show more details

3D structure databases

ProteinModelPortalⁱ	Q99816
SMRⁱ	Q99816
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ	Q99816

EvolutionaryTraceⁱ

Q99816

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	2 – 145	UEVPROSITE-ProRule annotationAdd BLAST		144
Domainⁱ	322 – 390	SBPROSITE-ProRule annotationAdd BLAST		69

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	158 – 162	Interaction with CEP55		5

Coiled coil

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Coiled coilⁱ	235 – 316	Sequence analysisAdd BLAST		82

Motif

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Motifⁱ	320 – 323	PTAP motif		4

Domainⁱ

The UEV domain is required for the interaction of the complex with ubiquitin. It also mediates the interaction with PTAP/PSAP motifs of HIV-1 P6 protein and human spumaretrovirus Gag protein.

The coiled coil domain may interact with stathmin.

The UEV domain binds ubiquitin and P-[ST]-A-P peptide motif independently.

Sequence similaritiesⁱ

Belongs to the ubiquitin-conjugating enzyme family. UEV subfamily.Curated

Keywords - Domainⁱ

Coiled coil

Phylogenomic databases

eggNOGⁱ	KOG2391 Eukaryota ENOG410XT2Q LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00530000064004
HOGENOMⁱ	HOG000247008
HOVERGENⁱ	HBG057450
InParanoidⁱ	Q99816
KEGG Orthology (KO) More...KOⁱ	K12183
OMAⁱ	DHNGKVY
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0HRA
PhylomeDBⁱ	Q99816
TreeFamⁱ	TF312917

Family and domain databases

Gene3Dⁱ	3.10.110.10, 1 hit
InterProⁱ	View protein in InterPro IPR037202 ESCRT_assembly_dom IPR017916 SB_dom IPR016135 UBQ-conjugating_enzyme/RWD IPR008883 UEV_N
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF05743 UEV, 1 hit PF09454 Vps23_core, 1 hit
SUPFAMⁱ	SSF140111 SSF140111, 1 hit SSF54495 SSF54495, 1 hit
PROSITEⁱ	View protein in PROSITE PS51312 SB, 1 hit PS51322 UEV, 1 hit

Sequences (2+)ⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsⁱ produced by alternative splicing. Align Add to basket

Note: Additional isoforms seem to exist. Several shorter isoforms are detected in primary breast cancers and other tumors.

This entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show all Align All

Isoform 1 (identifier: Q99816-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAVSESQLKK MVSKYKYRDL TVRETVNVIT LYKDLKPVLD SYVFNDGSSR 
        60         70         80         90        100
ELMNLTGTIP VPYRGNTYNI PICLWLLDTY PYNPPICFVK PTSSMTIKTG 
       110        120        130        140        150
KHVDANGKIY LPYLHEWKHP QSDLLGLIQV MIVVFGDEPP VFSRPISASY 
       160        170        180        190        200
PPYQATGPPN TSYMPGMPGG ISPYPSGYPP NPSGYPGCPY PPGGPYPATT 
       210        220        230        240        250
SSQYPSQPPV TTVGPSRDGT ISEDTIRASL ISAVSDKLRW RMKEEMDRAQ 
       260        270        280        290        300
AELNALKRTE EDLKKGHQKL EEMVTRLDQE VAEVDKNIEL LKKKDEELSS 
       310        320        330        340        350
ALEKMENQSE NNDIDEVIIP TAPLYKQILN LYAEENAIED TIFYLGEALR 
       360        370        380        390
RGVIDLDVFL KHVRLLSRKQ FQLRALMQKA RKTAGLSDLY

Length:390

Mass (Da):43,944

Last modified:August 1, 1998 - v2

Checksum:ⁱADD6912FC22DF162

GO

Isoform 2 (identifier: Q99816-2) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
15-119: Missing.

« Hide

        10         20         30         40         50
MAVSESQLKK MVSKPQSDLL GLIQVMIVVF GDEPPVFSRP ISASYPPYQA 
        60         70         80         90        100
TGPPNTSYMP GMPGGISPYP SGYPPNPSGY PGCPYPPGGP YPATTSSQYP 
       110        120        130        140        150
SQPPVTTVGP SRDGTISEDT IRASLISAVS DKLRWRMKEE MDRAQAELNA 
       160        170        180        190        200
LKRTEEDLKK GHQKLEEMVT RLDQEVAEVD KNIELLKKKD EELSSALEKM 
       210        220        230        240        250
ENQSENNDID EVIIPTAPLY KQILNLYAEE NAIEDTIFYL GEALRRGVID 
       260        270        280 
LDVFLKHVRL LSRKQFQLRA LMQKARKTAG LSDLY

Note: Detected in normal as well as cancer tissues.

Show »

Length:285

Mass (Da):31,732

Checksum:ⁱ035C70BEC887273D

GO

Computationally mapped potential isoform sequencesⁱ

There are 3 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

F5H442	F5H442_HUMAN	Tumor susceptibility gene 101 prote... Tumor susceptibility gene 101 protein	TSG101	365	Annotation score:
J3QRU6	J3QRU6_HUMAN	Tumor susceptibility gene 101 prote... Tumor susceptibility gene 101 protein	TSG101	63	Annotation score:
J3QKS4	J3QKS4_HUMAN	Tumor susceptibility gene 101 prote... Tumor susceptibility gene 101 protein	TSG101	77	Annotation score:

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	343	F → L in AAH02487 (PubMed:15489334).Curated		1

Natural variant

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Natural variantⁱVAR_034572	167	M → I. Corresponds to variant dbSNP:rs34385327Ensembl.		1

Alternative sequence

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Alternative sequenceⁱVSP_004440	15 – 119	Missing in isoform 2. CuratedAdd BLAST		105

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U82130 mRNA Translation: AAC52083.1 BC002487 mRNA Translation: AAH02487.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS7842.1 [Q99816-1]
NCBI Reference Sequences More...RefSeqⁱ	NP_006283.1, NM_006292.3 [Q99816-1]
UniGeneⁱ	Hs.523512

Genome annotation databases

Ensemblⁱ	ENST00000251968; ENSP00000251968; ENSG00000074319 [Q99816-1]
GeneIDⁱ	7251
KEGGⁱ	hsa:7251
UCSC genome browser More...UCSCⁱ	uc001mor.4 human [Q99816-1]

Keywords - Coding sequence diversityⁱ

Alternative splicing, Polymorphism

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q99816	TSG101 isoform 1		PANTR		390	UniRef100_Q99816
ESCRT-I complex subunit TSG101		MACMU		390
ESCRT-I complex subunit TSG101		MACFA		390
Uncharacterized protein		RHIRO		390
Uncharacterized protein		PAPAN		390
+38

Protein	Similar proteins		Species	Score	Length	Source
Q99816	Tumor susceptibility gene 101 protein		MOUSE		391	UniRef90_Q99816
Tumor susceptibility gene 101 protein		RAT		391
TSG101 isoform 1		PANTR		390
ESCRT-I complex subunit TSG101		MACMU		390
ESCRT-I complex subunit TSG101		MACFA		390
+200
Q99816-2	Tumor susceptibility gene 101 protein (Fragment)		MOUSE		148	UniRef90_Q99816-2
UPI0003D74854		MOUSE		356

Protein	Similar proteins		Species	Score	Length	Source
Q99816	Tumor susceptibility gene 101 protein		MOUSE		391	UniRef50_Q99816
Tumor susceptibility gene 101 protein		RAT		391
TSG101 isoform 1		PANTR		390
ESCRT-I complex subunit TSG101		MACMU		390
ESCRT-I complex subunit TSG101		MACFA		390
+389
Q99816-2	Tumor susceptibility gene 101 protein (Fragment)		MOUSE		148	UniRef50_Q99816-2
Tumor susceptibility protein 101 (Fragment)		MACMU		342
LOW QUALITY PROTEIN: tumor susceptibility gene 101 protein-like		PHYCD		421
Tumor susceptibility protein 101 protein		AMAAE		369
tumor susceptibility gene 101 protein isoform X2		ICTPU		369
+44

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U82130 mRNA Translation: AAC52083.1 BC002487 mRNA Translation: AAH02487.1
CCDSⁱ	CCDS7842.1 [Q99816-1]
RefSeqⁱ	NP_006283.1, NM_006292.3 [Q99816-1]
UniGeneⁱ	Hs.523512

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1KPP	NMR	-	A	1-145	[»]
	1KPQ	NMR	-	A	1-145	[»]
	1M4P	NMR	-	A	1-145	[»]
	1M4Q	NMR	-	A	1-145	[»]
	1S1Q	X-ray	2.00	A/C	1-145	[»]
	2F0R	X-ray	2.26	A/B	1-145	[»]
	3IV1	X-ray	2.50	A/B/C/D/E/F/G/H	229-304	[»]
	3OBQ	X-ray	1.40	A	2-145	[»]
	3OBS	X-ray	1.50	A	2-145	[»]
	3OBU	X-ray	1.60	A	2-145	[»]
	3OBX	X-ray	1.60	A	2-145	[»]
	3P9G	X-ray	1.80	A	2-145	[»]
	3P9H	X-ray	1.80	A	2-145	[»]
	4EJE	X-ray	2.20	A/B	1-145	[»]
	4YC1	X-ray	2.00	A/B/C	1-145	[»]
	4ZNY	X-ray	2.40	A	4-145	[»]
	5VKG	NMR	-	A	2-145	[»]
ProteinModelPortalⁱ	Q99816
SMRⁱ	Q99816
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	113102, 175 interactors
CORUMⁱ	Q99816
DIPⁱ	DIP-31809N
ELMⁱ	Q99816
IntActⁱ	Q99816, 135 interactors
MINTⁱ	Q99816
STRINGⁱ	9606.ENSP00000251968

Chemistry databases

BindingDBⁱ	Q99816
ChEMBLⁱ	CHEMBL6157

Protein family/group databases

MoonDBⁱ	Q99816 Curated

MoonDBⁱ

Q99816 Curated

PTM databases

iPTMnetⁱ	Q99816
PhosphoSitePlusⁱ	Q99816

Polymorphism and mutation databases

BioMutaⁱ	TSG101
DMDMⁱ	9789790

Proteomic databases

EPDⁱ	Q99816
MaxQBⁱ	Q99816
PaxDbⁱ	Q99816
PeptideAtlasⁱ	Q99816
PRIDEⁱ	Q99816
ProteomicsDBⁱ	78490 78491 [Q99816-2]

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	7251
Structural Biology Knowledgebase	Search...

Genome annotation databases

Ensemblⁱ	ENST00000251968; ENSP00000251968; ENSG00000074319 [Q99816-1]
GeneIDⁱ	7251
KEGGⁱ	hsa:7251
UCSCⁱ	uc001mor.4 human [Q99816-1]

Organism-specific databases

CTDⁱ	7251
DisGeNETⁱ	7251
EuPathDBⁱ	HostDB:ENSG00000074319.12
GeneCardsⁱ	TSG101
HGNCⁱ	HGNC:15971 TSG101
HPAⁱ	CAB004283 HPA006161
MalaCardsⁱ	TSG101
MIMⁱ	601387 gene
neXtProtⁱ	NX_Q99816
OpenTargetsⁱ	ENSG00000074319
PharmGKBⁱ	PA38068
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	KOG2391 Eukaryota ENOG410XT2Q LUCA
GeneTreeⁱ	ENSGT00530000064004
HOGENOMⁱ	HOG000247008
HOVERGENⁱ	HBG057450
InParanoidⁱ	Q99816
KOⁱ	K12183
OMAⁱ	DHNGKVY
OrthoDBⁱ	EOG091G0HRA
PhylomeDBⁱ	Q99816
TreeFamⁱ	TF312917

Enzyme and pathway databases

Reactomeⁱ	R-HSA-162588 Budding and maturation of HIV virion R-HSA-174490 Membrane binding and targetting of GAG proteins R-HSA-917729 Endosomal Sorting Complex Required For Transport (ESCRT)

Reactomeⁱ

R-HSA-162588 Budding and maturation of HIV virion
R-HSA-174490 Membrane binding and targetting of GAG proteins
R-HSA-917729 Endosomal Sorting Complex Required For Transport (ESCRT)

Miscellaneous databases

ChiTaRSⁱ	TSG101 human
EvolutionaryTraceⁱ	Q99816
GeneWikiⁱ	TSG101
GenomeRNAiⁱ	7251
Protein Ontology More...PROⁱ	PR:Q99816
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000074319 Expressed in 229 organ(s), highest expression level in oocyte
CleanExⁱ	HS_TSG101
ExpressionAtlasⁱ	Q99816 baseline and differential
Genevisibleⁱ	Q99816 HS

Family and domain databases

Gene3Dⁱ	3.10.110.10, 1 hit
InterProⁱ	View protein in InterPro IPR037202 ESCRT_assembly_dom IPR017916 SB_dom IPR016135 UBQ-conjugating_enzyme/RWD IPR008883 UEV_N
Pfamⁱ	View protein in Pfam PF05743 UEV, 1 hit PF09454 Vps23_core, 1 hit
SUPFAMⁱ	SSF140111 SSF140111, 1 hit SSF54495 SSF54495, 1 hit
PROSITEⁱ	View protein in PROSITE PS51312 SB, 1 hit PS51322 UEV, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	TS101_HUMAN
Accessionⁱ	Q99816Primary (citable) accession number: Q99816 Secondary accession number(s): Q9BUM5
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
	Last sequence update:	August 1, 1998
	Last modified:	November 7, 2018
	This is version 208 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations
Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
PDB cross-references
Index of Protein Data Bank (PDB) cross-references
Human chromosome 11
Human chromosome 11: entries, gene names and cross-references to MIM

UniProtKB

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UniProtKB - Q99816 (TS101_HUMAN)

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Tumor susceptibility gene 101 protein

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

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GO - Biological processi

Enzyme and pathway databases

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Cytoskeleton

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Other locations

Cytoskeleton

Cytosol

Endosome

Extracellular region or secreted

Nucleus

Plasma Membrane

Other locations

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Organism-specific databases

Chemistry databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

Chemistry databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

Coiled coil

Motif

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Experimental Info

Natural variant

Alternative sequence

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Chemistry databases

Protein family/group databases

PTM databases

Polymorphism and mutation databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ