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UniProtKB - Q99816 (TS101_HUMAN)

Protein

Tumor susceptibility gene 101 protein

Gene

TSG101

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses. Required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413). It may also play a role in the extracellular release of microvesicles that differ from the exosomes (PubMed:22315426).7 Publications

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

View the complete GO annotation on QuickGO ...

GO - Biological processi

View the complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Biological processCell cycle, Cell division, Growth regulation, Host-virus interaction, Protein transport, Transport

Enzyme and pathway databases

Reactome - a knowledgebase of biological pathways and processes

More...Reactomei		R-HSA-162588 Budding and maturation of HIV virion
R-HSA-174490 Membrane binding and targetting of GAG proteins
R-HSA-917729 Endosomal Sorting Complex Required For Transport (ESCRT)

Protein family/group databases

MoonDB Database of extreme multifunctional and moonlighting proteins

More...MoonDBi		Q99816 Curated

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Tumor susceptibility gene 101 protein
Alternative name(s):
ESCRT-I complex subunit TSG101
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:TSG101
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiHomo sapiens (Human)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri9606 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000005640 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 11

Organism-specific databases

Eukaryotic Pathogen Database Resources

More...EuPathDBi		HostDB:ENSG00000074319.12

Human Gene Nomenclature Database

More...HGNCi		HGNC:15971 TSG101

Online Mendelian Inheritance in Man (OMIM)

More...MIMi		601387 gene

neXtProt; the human protein knowledge platform

More...neXtProti		NX_Q99816

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Endosome, Membrane, Nucleus

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology_and_biotech_section">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi43V → A: Reduces interaction with ubiquitin; inhibits down-regulation of EGFR. 1 Publication1
Mutagenesisi45N → A: Reduces interaction with ubiquitin. No effect on MGRN1-binding. 3 Publications1
Mutagenesisi46D → A: Reduces interaction with ubiquitin. 1 Publication1
Mutagenesisi63Y → A: Reduces interaction with HIV-1 p6; impairs HIV-1 budding. 1 Publication1
Mutagenesisi88F → A: Reduces interaction with ubiquitin; no effect on in interaction with HIV-1 p6. 1 Publication1
Mutagenesisi89V → A: No change in interaction with p6; no effect on HIV-1 budding. 1 Publication1
Mutagenesisi95M → A: Reduces interaction with VPS37B and HIV-1 p6; abolishes interaction with PDCD6IP; impairs HIV-1 budding; inhibits down-regulation of EGFR. Abolishes MGRN1-binding. Loss of interaction with ARRDC1. 6 Publications1
Mutagenesisi141V → A: Reduces interaction with HIV-1 p6. 1 Publication1
Mutagenesisi158 – 162Missing : Abolishes interaction with CEP55 and midbody localization; no effect on interaction with ESCRT-I proteins, PDCD6IP and viral proteins. 1 Publication5
Mutagenesisi158 – 160PPN → AAA: Abolishes interaction with CEP55. 1 Publication3
Mutagenesisi368 – 371RKQF → AAAA: Loss of interaction with VPS28. No effect on interaction with VPS37C. 1 Publication4

Organism-specific databases

DisGeNET

More...DisGeNETi		7251

MalaCards human disease database

More...MalaCardsi		TSG101

Open Targets

More...OpenTargetsi		ENSG00000074319

The Pharmacogenetics and Pharmacogenomics Knowledge Base

More...PharmGKBi		PA38068

Chemistry databases

ChEMBL database of bioactive drug-like small molecules

More...ChEMBLi		CHEMBL6157

Polymorphism and mutation databases

BioMuta curated single-nucleotide variation and disease association database

More...BioMutai		TSG101

Domain mapping of disease mutations (DMDM)

More...DMDMi		9789790

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/ptm_processing_section">PTM / Processing</a> section indicates that the initiator methionine is cleaved from the mature protein.<p><a href='/help/init_met' target='_top'>More...</a></p>Initiator methionineiRemovedCombined sources
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000826062 – 390Tumor susceptibility gene 101 proteinAdd BLAST389

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei2N-acetylalanineCombined sources1
Modified residuei220PhosphothreonineCombined sources1

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm_processing_section">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

Monoubiquitinated at multiple sites by LRSAM1 and by MGRN1. Ubiquitination inactivates it, possibly by regulating its shuttling between an active membrane-bound protein and an inactive soluble form. Ubiquitination by MGRN1 requires the presence of UBE2D1.3 Publications

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

Encyclopedia of Proteome Dynamics

More...EPDi		Q99816

jPOST - Japan Proteome Standard Repository/Database

More...jPOSTi		Q99816

MaxQB - The MaxQuant DataBase

More...MaxQBi		Q99816

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		Q99816

PeptideAtlas

More...PeptideAtlasi		Q99816

PRoteomics IDEntifications database

More...PRIDEi		Q99816

ProteomicsDB human proteome resource

More...ProteomicsDBi		78490
78491 [Q99816-2]

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...iPTMneti		Q99816

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

More...PhosphoSitePlusi		Q99816

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the ‘Expression’ section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified ‘at protein level’. <br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

Heart, brain, placenta, lung, liver, skeletal, kidney and pancreas.

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...Bgeei		ENSG00000074319 Expressed in 229 organ(s), highest expression level in oocyte

CleanEx database of gene expression profiles

More...CleanExi		HS_TSG101

ExpressionAtlas, Differential and Baseline Expression

More...ExpressionAtlasi		Q99816 baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

More...Genevisiblei		Q99816 HS

Organism-specific databases

Human Protein Atlas

More...HPAi		CAB004283
HPA006161

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Component of the ESCRT-I complex (endosomal sorting complex required for transport I) which consists of TSG101, VPS28, a VPS37 protein (VPS37A to -D) and MVB12A or MVB12B in a 1:1:1:1 stoichiometry (PubMed:18005716). Interacts with VPS37A, VPS37B and VPS37C (PubMed:15218037, PubMed:15509564). Interacts with DMAP1 (PubMed:10888872). Interacts with ubiquitin (PubMed:11595185). Interacts with stathmin, GMCL and AATF (By similarity). Component of an ESCRT-I complex (endosomal sorting complex required for transport I) which consists of TSG101, VPS28, VPS37A and UBAP1 in a 1:1:1:1 stoichiometry (PubMed:21757351). Interacts with HGS; the interaction mediates the association with the ESCRT-0 complex. Interacts with GGA1 and GGA3 (PubMed:15143060, PubMed:15039775). Interacts (via UEV domain) with PDCD6IP/AIP1 (PubMed:14505570, PubMed:14519844). Interacts with VPS28, SNF8 and VPS36 (PubMed:14505570). Self-associates (PubMed:14505570, PubMed:14519844). Interacts with MVB12A; the association appears to be mediated by the TSG101-VPS37 binary subcomplex. Interacts with VPS37D. Interacts with LRSAM1. Interacts with CEP55; the interaction is required for cytokinesis but not for viral budding (PubMed:17853893). Interacts with PDCD6 (PubMed:18256029). Interacts with LITAF (PubMed:23166352). Interacts with MGRN1 (PubMed:17229889). Interacts with ARRDC1; recruits TSG101 to the plasma membrane (PubMed:21191027, PubMed:22315426).By similarity19 Publications
(Microbial infection) Interacts with HIV-1 p6.5 Publications
(Microbial infection) Interacts with human spumavirus Gag.1 Publication
(Microbial infection) Interacts with HTLV-1 Gag.1 Publication
(Microbial infection) Interacts with Ebola virus VP40.1 Publication
(Microbial infection) Interacts with EIAV p9; the interaction has been shown in vitro.1 Publication
(Microbial infection) Interacts with Lassa virus protein Z.1 Publication
(Microbial infection) Interacts with hepatitis E virus protein ORF3.1 Publication

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction_section%27">Interaction</a> section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="http://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated on a monthly basis. Each binary interaction is displayed on a separate line.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGrid)

More...BioGridi		113102, 186 interactors

CORUM comprehensive resource of mammalian protein complexes

More...CORUMi		Q99816

Database of interacting proteins

More...DIPi		DIP-31809N

The Eukaryotic Linear Motif resource for Functional Sites in Proteins

More...ELMi		Q99816

Protein interaction database and analysis system

More...IntActi		Q99816, 135 interactors

Molecular INTeraction database

More...MINTi		Q99816

STRING: functional protein association networks

More...STRINGi		9606.ENSP00000251968

Chemistry databases

BindingDB database of measured binding affinities

More...BindingDBi		Q99816

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1390
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...PDBei

Protein Data Bank RCSB

More...RCSB PDBi

Protein Data Bank Japan

More...PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1KPPNMR-A1-145[»]
1KPQNMR-A1-145[»]
1M4PNMR-A1-145[»]
1M4QNMR-A1-145[»]
1S1QX-ray2.00A/C1-145[»]
2F0RX-ray2.26A/B1-145[»]
3IV1X-ray2.50A/B/C/D/E/F/G/H229-304[»]
3OBQX-ray1.40A2-145[»]
3OBSX-ray1.50A2-145[»]
3OBUX-ray1.60A2-145[»]
3OBXX-ray1.60A2-145[»]
3P9GX-ray1.80A2-145[»]
3P9HX-ray1.80A2-145[»]
4EJEX-ray2.20A/B1-145[»]
4YC1X-ray2.00A/B/C1-145[»]
4ZNYX-ray2.40A4-145[»]
5VKGNMR-A2-145[»]

Protein Model Portal of the PSI-Nature Structural Biology Knowledgebase

More...ProteinModelPortali		Q99816

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		Q99816

Database of comparative protein structure models

More...ModBasei		Search...

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

More...EvolutionaryTracei		Q99816

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini2 – 145UEVPROSITE-ProRule annotationAdd BLAST144
Domaini322 – 390SBPROSITE-ProRule annotationAdd BLAST69

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and Domains’ section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni158 – 162Interaction with CEP555

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and domains’ section denotes the positions of regions of coiled coil within the protein.<p><a href='/help/coiled' target='_top'>More...</a></p>Coiled coili235 – 316Sequence analysisAdd BLAST82

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and Domains’ section describes a short (usually not more than 20 amino acids) conserved sequence motif of biological significance.<p><a href='/help/motif' target='_top'>More...</a></p>Motifi320 – 323PTAP motif4

<p>This subsection of the ‘Family and domains’ section provides general information on the biological role of a domain. The term ‘domain’ is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

The UEV domain is required for the interaction of the complex with ubiquitin. It also mediates the interaction with PTAP/PSAP motifs of HIV-1 P6 protein and human spumaretrovirus Gag protein.
The coiled coil domain may interact with stathmin.
The UEV domain binds ubiquitin and P-[ST]-A-P peptide motif independently.

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family. UEV subfamily.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		KOG2391 Eukaryota
ENOG410XT2Q LUCA

Ensembl GeneTree

More...GeneTreei		ENSGT00940000153903

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		HOG000247008

The HOVERGEN Database of Homologous Vertebrate Genes

More...HOVERGENi		HBG057450

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		Q99816

KEGG Orthology (KO)

More...KOi		K12183

Identification of Orthologs from Complete Genome Data

More...OMAi		DHNGKVY

Database of Orthologous Groups

More...OrthoDBi		1435538at2759

Database for complete collections of gene phylogenies

More...PhylomeDBi		Q99816

TreeFam database of animal gene trees

More...TreeFami		TF312917

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...Gene3Di		3.10.110.10, 1 hit

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR037202 ESCRT_assembly_dom
IPR017916 SB_dom
IPR016135 UBQ-conjugating_enzyme/RWD
IPR008883 UEV_N

Pfam protein domain database

More...Pfami		View protein in Pfam
PF05743 UEV, 1 hit
PF09454 Vps23_core, 1 hit

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF140111 SSF140111, 1 hit
SSF54495 SSF54495, 1 hit

PROSITE; a protein domain and family database

More...PROSITEi		View protein in PROSITE
PS51312 SB, 1 hit
PS51322 UEV, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (2+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is in its mature form or if it represents the precursor.<p><a href='/help/sequence_processing' target='_top'>More...</a></p>Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 <p>This subsection of the ‘Sequence’ section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket
Note: Additional isoforms seem to exist. Several shorter isoforms are detected in primary breast cancers and other tumors.

This entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q99816-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAVSESQLKK MVSKYKYRDL TVRETVNVIT LYKDLKPVLD SYVFNDGSSR 
        60         70         80         90        100
ELMNLTGTIP VPYRGNTYNI PICLWLLDTY PYNPPICFVK PTSSMTIKTG 
       110        120        130        140        150
KHVDANGKIY LPYLHEWKHP QSDLLGLIQV MIVVFGDEPP VFSRPISASY 
       160        170        180        190        200
PPYQATGPPN TSYMPGMPGG ISPYPSGYPP NPSGYPGCPY PPGGPYPATT 
       210        220        230        240        250
SSQYPSQPPV TTVGPSRDGT ISEDTIRASL ISAVSDKLRW RMKEEMDRAQ 
       260        270        280        290        300
AELNALKRTE EDLKKGHQKL EEMVTRLDQE VAEVDKNIEL LKKKDEELSS 
       310        320        330        340        350
ALEKMENQSE NNDIDEVIIP TAPLYKQILN LYAEENAIED TIFYLGEALR 
       360        370        380        390
RGVIDLDVFL KHVRLLSRKQ FQLRALMQKA RKTAGLSDLY
Length:390
Mass (Da):43,944
Last modified:August 1, 1998 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iADD6912FC22DF162
GO
Isoform 2 (identifier: Q99816-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     15-119: Missing.

Note: Detected in normal as well as cancer tissues.
Show »
Length:285
Mass (Da):31,732
Checksum:i035C70BEC887273D
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F5H442F5H442_HUMAN
Tumor susceptibility gene 101 prote...
TSG101
365Annotation score:

Annotation score:2 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
J3QKS4J3QKS4_HUMAN
Tumor susceptibility gene 101 prote...
TSG101
77Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
J3QRU6J3QRU6_HUMAN
Tumor susceptibility gene 101 prote...
TSG101
63Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti343F → L in AAH02487 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section describes natural variant(s) of the protein sequence.<p><a href='/help/variant' target='_top'>More...</a></p>Natural variantiVAR_034572167M → I. Corresponds to variant dbSNP:rs34385327Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting. The information stored in this subsection is used to automatically construct alternative protein sequence(s) for display.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_00444015 – 119Missing in isoform 2. CuratedAdd BLAST105

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
U82130 mRNA Translation: AAC52083.1
BC002487 mRNA Translation: AAH02487.1

The Consensus CDS (CCDS) project

More...CCDSi		CCDS7842.1 [Q99816-1]

NCBI Reference Sequences

More...RefSeqi		NP_006283.1, NM_006292.3 [Q99816-1]

UniGene gene-oriented nucleotide sequence clusters

More...UniGenei		Hs.523512

Genome annotation databases

Ensembl eukaryotic genome annotation project

More...Ensembli		ENST00000251968; ENSP00000251968; ENSG00000074319 [Q99816-1]

Database of genes from NCBI RefSeq genomes

More...GeneIDi		7251

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		hsa:7251

UCSC genome browser

More...UCSCi		uc001mor.4 human [Q99816-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U82130 mRNA Translation: AAC52083.1
BC002487 mRNA Translation: AAH02487.1
CCDSiCCDS7842.1 [Q99816-1]
RefSeqiNP_006283.1, NM_006292.3 [Q99816-1]
UniGeneiHs.523512

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1KPPNMR-A1-145[»]
1KPQNMR-A1-145[»]
1M4PNMR-A1-145[»]
1M4QNMR-A1-145[»]
1S1QX-ray2.00A/C1-145[»]
2F0RX-ray2.26A/B1-145[»]
3IV1X-ray2.50A/B/C/D/E/F/G/H229-304[»]
3OBQX-ray1.40A2-145[»]
3OBSX-ray1.50A2-145[»]
3OBUX-ray1.60A2-145[»]
3OBXX-ray1.60A2-145[»]
3P9GX-ray1.80A2-145[»]
3P9HX-ray1.80A2-145[»]
4EJEX-ray2.20A/B1-145[»]
4YC1X-ray2.00A/B/C1-145[»]
4ZNYX-ray2.40A4-145[»]
5VKGNMR-A2-145[»]
ProteinModelPortaliQ99816
SMRiQ99816
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi113102, 186 interactors
CORUMiQ99816
DIPiDIP-31809N
ELMiQ99816
IntActiQ99816, 135 interactors
MINTiQ99816
STRINGi9606.ENSP00000251968

Chemistry databases

BindingDBiQ99816
ChEMBLiCHEMBL6157

Protein family/group databases

MoonDBiQ99816 Curated

PTM databases

iPTMnetiQ99816
PhosphoSitePlusiQ99816

Polymorphism and mutation databases

BioMutaiTSG101
DMDMi9789790

Proteomic databases

EPDiQ99816
jPOSTiQ99816
MaxQBiQ99816
PaxDbiQ99816
PeptideAtlasiQ99816
PRIDEiQ99816
ProteomicsDBi78490
78491 [Q99816-2]

Protocols and materials databases

The DNASU plasmid repository

More...DNASUi		7251
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000251968; ENSP00000251968; ENSG00000074319 [Q99816-1]
GeneIDi7251
KEGGihsa:7251
UCSCiuc001mor.4 human [Q99816-1]

Organism-specific databases

Comparative Toxicogenomics Database

More...CTDi		7251
DisGeNETi7251
EuPathDBiHostDB:ENSG00000074319.12

GeneCards: human genes, protein and diseases

More...GeneCardsi		TSG101
HGNCiHGNC:15971 TSG101
HPAiCAB004283
HPA006161
MalaCardsiTSG101
MIMi601387 gene
neXtProtiNX_Q99816
OpenTargetsiENSG00000074319
PharmGKBiPA38068

GenAtlas: human gene database

More...GenAtlasi		Search...

Phylogenomic databases

eggNOGiKOG2391 Eukaryota
ENOG410XT2Q LUCA
GeneTreeiENSGT00940000153903
HOGENOMiHOG000247008
HOVERGENiHBG057450
InParanoidiQ99816
KOiK12183
OMAiDHNGKVY
OrthoDBi1435538at2759
PhylomeDBiQ99816
TreeFamiTF312917

Enzyme and pathway databases

ReactomeiR-HSA-162588 Budding and maturation of HIV virion
R-HSA-174490 Membrane binding and targetting of GAG proteins
R-HSA-917729 Endosomal Sorting Complex Required For Transport (ESCRT)

Miscellaneous databases

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

More...ChiTaRSi		TSG101 human
EvolutionaryTraceiQ99816

The Gene Wiki collection of pages on human genes and proteins

More...GeneWikii		TSG101

Database of phenotypes from RNA interference screens in Drosophila and Homo sapiens

More...GenomeRNAii		7251

Protein Ontology

More...PROi		PR:Q99816

The Stanford Online Universal Resource for Clones and ESTs

More...SOURCEi		Search...

Gene expression databases

BgeeiENSG00000074319 Expressed in 229 organ(s), highest expression level in oocyte
CleanExiHS_TSG101
ExpressionAtlasiQ99816 baseline and differential
GenevisibleiQ99816 HS

Family and domain databases

Gene3Di3.10.110.10, 1 hit
InterProiView protein in InterPro
IPR037202 ESCRT_assembly_dom
IPR017916 SB_dom
IPR016135 UBQ-conjugating_enzyme/RWD
IPR008883 UEV_N
PfamiView protein in Pfam
PF05743 UEV, 1 hit
PF09454 Vps23_core, 1 hit
SUPFAMiSSF140111 SSF140111, 1 hit
SSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS51312 SB, 1 hit
PS51322 UEV, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...ProtoNeti		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiTS101_HUMAN
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q99816
Secondary accession number(s): Q9BUM5
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: August 1, 1998
Last modified: January 16, 2019
This is version 210 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
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