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Protein

Equilibrative nucleoside transporter 1

Gene

SLC29A1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Mediates both influx and efflux of nucleosides across the membrane (equilibrative transporter). It is sensitive (ES) to low concentrations of the inhibitor nitrobenzylmercaptopurine riboside (NBMPR) and is sodium-independent. It has a higher affinity for adenosine. Inhibited by dipyridamole and dilazep (anticancer chemotherapeutics drugs).

Kineticsi

Vmax for adenosine uptake is about the same for SLC29A1 and SLC29A2.
  1. KM=0.62 mM for adenosine1 Publication

    GO - Molecular functioni

    • nucleoside transmembrane transporter activity Source: GO_Central

    GO - Biological processi

    Keywordsi

    Biological processTransport

    Enzyme and pathway databases

    ReactomeiR-HSA-83936 Transport of nucleosides and free purine and pyrimidine bases across the plasma membrane

    Protein family/group databases

    TCDBi2.A.57.1.1 the equilibrative nucleoside transporter (ent) family

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Equilibrative nucleoside transporter 1
    Alternative name(s):
    Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter
    Short name:
    Equilibrative NBMPR-sensitive nucleoside transporter
    Nucleoside transporter, es-type
    Solute carrier family 29 member 1
    Gene namesi
    Name:SLC29A1
    Synonyms:ENT1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    Proteomesi
    • UP000005640 Componenti: Chromosome 6

    Organism-specific databases

    EuPathDBiHostDB:ENSG00000112759.16
    HGNCiHGNC:11003 SLC29A1
    MIMi602193 gene
    neXtProtiNX_Q99808

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Topological domaini2 – 12CytoplasmicSequence analysisAdd BLAST11
    Transmembranei13 – 29HelicalSequence analysisAdd BLAST17
    Topological domaini30 – 82ExtracellularSequence analysisAdd BLAST53
    Transmembranei83 – 107HelicalSequence analysisAdd BLAST25
    Topological domaini108 – 111CytoplasmicSequence analysis4
    Transmembranei112 – 130HelicalSequence analysisAdd BLAST19
    Topological domaini131 – 138ExtracellularSequence analysis8
    Transmembranei139 – 157HelicalSequence analysisAdd BLAST19
    Topological domaini158 – 174CytoplasmicSequence analysisAdd BLAST17
    Transmembranei175 – 199HelicalSequence analysisAdd BLAST25
    Topological domaini200 – 206ExtracellularSequence analysis7
    Transmembranei207 – 227HelicalSequence analysisAdd BLAST21
    Topological domaini228 – 291CytoplasmicSequence analysisAdd BLAST64
    Transmembranei292 – 311HelicalSequence analysisAdd BLAST20
    Topological domaini312 – 323ExtracellularSequence analysisAdd BLAST12
    Transmembranei324 – 342HelicalSequence analysisAdd BLAST19
    Topological domaini343 – 359CytoplasmicSequence analysisAdd BLAST17
    Transmembranei360 – 378HelicalSequence analysisAdd BLAST19
    Topological domaini379 – 393ExtracellularSequence analysisAdd BLAST15
    Transmembranei394 – 413HelicalSequence analysisAdd BLAST20
    Topological domaini414 – 431CytoplasmicSequence analysisAdd BLAST18
    Transmembranei432 – 452HelicalSequence analysisAdd BLAST21
    Topological domaini453 – 456ExtracellularSequence analysis4

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi453 – 456Missing : No effect on subcellular localization and inosine transport. 1 Publication4
    Mutagenesisi453R → A: No effect on subcellular localization and inosine transport. 1 Publication1

    Organism-specific databases

    DisGeNETi2030
    OpenTargetsiENSG00000112759
    PharmGKBiPA154

    Chemistry databases

    ChEMBLiCHEMBL1997
    DrugBankiDB00987 Cytarabine
    DB00900 Didanosine
    DB00898 Ethanol
    DB01073 Fludarabine
    DB00544 Fluorouracil
    DB00441 Gemcitabine
    DB01033 Mercaptopurine
    DB00642 Pemetrexed
    DB00811 Ribavirin
    DB00197 Troglitazone
    DB02745 Uridine
    DB00943 Zalcitabine
    GuidetoPHARMACOLOGYi1117

    Polymorphism and mutation databases

    BioMutaiSLC29A1
    DMDMi9296956

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Initiator methionineiRemoved1 Publication
    ChainiPRO_00002093372 – 456Equilibrative nucleoside transporter 1Add BLAST455

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Glycosylationi48N-linked (GlcNAc...) asparagine1 Publication1
    Modified residuei254PhosphoserineCombined sources1
    Modified residuei269PhosphoserineCombined sources1
    Modified residuei273PhosphoserineCombined sources1

    Post-translational modificationi

    Glycosylated.1 Publication

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    EPDiQ99808
    MaxQBiQ99808
    PaxDbiQ99808
    PeptideAtlasiQ99808
    PRIDEiQ99808
    ProteomicsDBi78487

    PTM databases

    GlyConnecti1219
    iPTMnetiQ99808
    PhosphoSitePlusiQ99808
    SwissPalmiQ99808

    Expressioni

    Tissue specificityi

    Detected in erythrocytes (at protein level). Expressed in heart, brain, mammary gland, erythrocytes and placenta, and also in fetal liver and spleen.2 Publications

    Gene expression databases

    BgeeiENSG00000112759 Expressed in 190 organ(s), highest expression level in lower esophagus
    CleanExiHS_SLC29A1
    GenevisibleiQ99808 HS

    Organism-specific databases

    HPAiHPA012384

    Interactioni

    Subunit structurei

    Identified in a complex with STOM.1 Publication

    Protein-protein interaction databases

    BioGridi108344, 32 interactors
    IntActiQ99808, 8 interactors
    MINTiQ99808
    STRINGi9606.ENSP00000360773

    Chemistry databases

    BindingDBiQ99808

    Structurei

    3D structure databases

    ProteinModelPortaliQ99808
    SMRiQ99808
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG1479 Eukaryota
    ENOG410Y3MT LUCA
    GeneTreeiENSGT00390000002232
    HOGENOMiHOG000007684
    HOVERGENiHBG074626
    InParanoidiQ99808
    KOiK15014
    OMAiMTPWNFF
    OrthoDBiEOG091G09WB
    PhylomeDBiQ99808
    TreeFamiTF313950

    Family and domain databases

    InterProiView protein in InterPro
    IPR030195 ENT1
    IPR034764 ENT1/ENT2
    IPR002259 Eqnu_transpt
    IPR036259 MFS_trans_sf
    PANTHERiPTHR10332 PTHR10332, 1 hit
    PTHR10332:SF9 PTHR10332:SF9, 1 hit
    PfamiView protein in Pfam
    PF01733 Nucleoside_tran, 1 hit
    PIRSFiPIRSF016379 ENT, 1 hit
    PRINTSiPR01130 DERENTRNSPRT
    SUPFAMiSSF103473 SSF103473, 1 hit
    TIGRFAMsiTIGR00939 2a57, 1 hit

    Sequences (2+)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

    This entry has 2 described isoforms and 10 potential isoforms that are computationally mapped.Show allAlign All

    Isoform 1 (identifier: Q99808-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide
            10         20         30         40         50
    MTTSHQPQDR YKAVWLIFFM LGLGTLLPWN FFMTATQYFT NRLDMSQNVS
    60 70 80 90 100
    LVTAELSKDA QASAAPAAPL PERNSLSAIF NNVMTLCAML PLLLFTYLNS
    110 120 130 140 150
    FLHQRIPQSV RILGSLVAIL LVFLITAILV KVQLDALPFF VITMIKIVLI
    160 170 180 190 200
    NSFGAILQGS LFGLAGLLPA SYTAPIMSGQ GLAGFFASVA MICAIASGSE
    210 220 230 240 250
    LSESAFGYFI TACAVIILTI ICYLGLPRLE FYRYYQQLKL EGPGEQETKL
    260 270 280 290 300
    DLISKGEEPR AGKEESGVSV SNSQPTNESH SIKAILKNIS VLAFSVCFIF
    310 320 330 340 350
    TITIGMFPAV TVEVKSSIAG SSTWERYFIP VSCFLTFNIF DWLGRSLTAV
    360 370 380 390 400
    FMWPGKDSRW LPSLVLARLV FVPLLLLCNI KPRRYLTVVF EHDAWFIFFM
    410 420 430 440 450
    AAFAFSNGYL ASLCMCFGPK KVKPAEAETA GAIMAFFLCL GLALGAVFSF

    LFRAIV
    Length:456
    Mass (Da):50,219
    Last modified:January 23, 2007 - v3
    Checksum:iF18535A95DEBC95D
    GO
    Isoform 2 (identifier: Q99808-2) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-1: M → MRRERTRGPQ...PGKTENTITM

    Note: No experimental confirmation available.
    Show »
    Length:535
    Mass (Da):58,963
    Checksum:iE58D6674A3C519D7
    GO

    Computationally mapped potential isoform sequencesi

    There are 10 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
    EntryEntry nameProtein names
    Gene namesLengthAnnotation
    A0A2U3TZM6A0A2U3TZM6_HUMAN
    Equilibrative nucleoside transporte...
    SLC29A1
    210Annotation score:
    A0A2U3TZJ7A0A2U3TZJ7_HUMAN
    Equilibrative nucleoside transporte...
    SLC29A1
    229Annotation score:
    A0A2R8Y6D1A0A2R8Y6D1_HUMAN
    Equilibrative nucleoside transporte...
    SLC29A1
    248Annotation score:
    A0A2U3TZM5A0A2U3TZM5_HUMAN
    Equilibrative nucleoside transporte...
    SLC29A1
    177Annotation score:
    A0A2R8YFB9A0A2R8YFB9_HUMAN
    Equilibrative nucleoside transporte...
    SLC29A1
    136Annotation score:
    A0A2R8YFI6A0A2R8YFI6_HUMAN
    Equilibrative nucleoside transporte...
    SLC29A1
    169Annotation score:
    A0A2R8YEH8A0A2R8YEH8_HUMAN
    Equilibrative nucleoside transporte...
    SLC29A1
    83Annotation score:
    A0A2R8Y801A0A2R8Y801_HUMAN
    Equilibrative nucleoside transporte...
    SLC29A1
    110Annotation score:
    A0A2R8Y567A0A2R8Y567_HUMAN
    Equilibrative nucleoside transporte...
    SLC29A1
    78Annotation score:
    A0A2R8Y854A0A2R8Y854_HUMAN
    Equilibrative nucleoside transporte...
    SLC29A1
    164Annotation score:

    Natural variant

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Natural variantiVAR_053668216I → T. Corresponds to variant dbSNP:rs45573936Ensembl.1
    Natural variantiVAR_036221293A → T in a colorectal cancer sample; somatic mutation. 1 Publication1
    Natural variantiVAR_053669391E → K. Corresponds to variant dbSNP:rs45458701Ensembl.1
    Natural variantiVAR_036222455I → V in a colorectal cancer sample; somatic mutation. 1 PublicationCorresponds to variant dbSNP:rs767108156Ensembl.1

    Alternative sequence

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Alternative sequenceiVSP_0565791M → MRRERTRGPQAWEFPSPTKS GCSLQSLSRDLRELREGEKP EDQAETEESWQGLARKTPGK ACAPEGGSCQPGKTENTITM in isoform 2. 1 Publication1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U81375 mRNA Translation: AAC51103.1
    AF190884 Genomic DNA Translation: AAF02777.1
    AF079117 mRNA Translation: AAC62495.1
    AF495730 Genomic DNA Translation: AAM11785.1
    AK090491 mRNA Translation: BAG52169.1
    AK090615 mRNA Translation: BAG52197.1
    AL139392 Genomic DNA No translation available.
    CH471081 Genomic DNA Translation: EAX04255.1
    BC001382 mRNA Translation: AAH01382.1
    BC008954 mRNA Translation: AAH08954.1
    CCDSiCCDS4908.1 [Q99808-1]
    RefSeqiNP_001071643.1, NM_001078175.2 [Q99808-1]
    NP_001071645.1, NM_001078177.1 [Q99808-1]
    NP_001291391.1, NM_001304462.1 [Q99808-2]
    NP_001291392.1, NM_001304463.1
    NP_001291394.1, NM_001304465.1
    NP_001291395.1, NM_001304466.1
    XP_005248935.1, XM_005248878.3 [Q99808-1]
    XP_005248936.1, XM_005248879.3 [Q99808-1]
    XP_005248937.1, XM_005248880.3 [Q99808-1]
    XP_005248938.1, XM_005248881.3 [Q99808-1]
    XP_005248939.1, XM_005248882.3 [Q99808-1]
    UniGeneiHs.25450

    Genome annotation databases

    EnsembliENST00000371708; ENSP00000360773; ENSG00000112759 [Q99808-1]
    ENST00000371713; ENSP00000360778; ENSG00000112759 [Q99808-1]
    ENST00000371724; ENSP00000360789; ENSG00000112759 [Q99808-1]
    ENST00000371755; ENSP00000360820; ENSG00000112759 [Q99808-1]
    ENST00000393844; ENSP00000377427; ENSG00000112759 [Q99808-1]
    ENST00000427851; ENSP00000392668; ENSG00000112759 [Q99808-1]
    GeneIDi2030
    KEGGihsa:2030
    UCSCiuc003owu.2 human [Q99808-1]

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Similar proteinsi

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U81375 mRNA Translation: AAC51103.1
    AF190884 Genomic DNA Translation: AAF02777.1
    AF079117 mRNA Translation: AAC62495.1
    AF495730 Genomic DNA Translation: AAM11785.1
    AK090491 mRNA Translation: BAG52169.1
    AK090615 mRNA Translation: BAG52197.1
    AL139392 Genomic DNA No translation available.
    CH471081 Genomic DNA Translation: EAX04255.1
    BC001382 mRNA Translation: AAH01382.1
    BC008954 mRNA Translation: AAH08954.1
    CCDSiCCDS4908.1 [Q99808-1]
    RefSeqiNP_001071643.1, NM_001078175.2 [Q99808-1]
    NP_001071645.1, NM_001078177.1 [Q99808-1]
    NP_001291391.1, NM_001304462.1 [Q99808-2]
    NP_001291392.1, NM_001304463.1
    NP_001291394.1, NM_001304465.1
    NP_001291395.1, NM_001304466.1
    XP_005248935.1, XM_005248878.3 [Q99808-1]
    XP_005248936.1, XM_005248879.3 [Q99808-1]
    XP_005248937.1, XM_005248880.3 [Q99808-1]
    XP_005248938.1, XM_005248881.3 [Q99808-1]
    XP_005248939.1, XM_005248882.3 [Q99808-1]
    UniGeneiHs.25450

    3D structure databases

    ProteinModelPortaliQ99808
    SMRiQ99808
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi108344, 32 interactors
    IntActiQ99808, 8 interactors
    MINTiQ99808
    STRINGi9606.ENSP00000360773

    Chemistry databases

    BindingDBiQ99808
    ChEMBLiCHEMBL1997
    DrugBankiDB00987 Cytarabine
    DB00900 Didanosine
    DB00898 Ethanol
    DB01073 Fludarabine
    DB00544 Fluorouracil
    DB00441 Gemcitabine
    DB01033 Mercaptopurine
    DB00642 Pemetrexed
    DB00811 Ribavirin
    DB00197 Troglitazone
    DB02745 Uridine
    DB00943 Zalcitabine
    GuidetoPHARMACOLOGYi1117

    Protein family/group databases

    TCDBi2.A.57.1.1 the equilibrative nucleoside transporter (ent) family

    PTM databases

    GlyConnecti1219
    iPTMnetiQ99808
    PhosphoSitePlusiQ99808
    SwissPalmiQ99808

    Polymorphism and mutation databases

    BioMutaiSLC29A1
    DMDMi9296956

    Proteomic databases

    EPDiQ99808
    MaxQBiQ99808
    PaxDbiQ99808
    PeptideAtlasiQ99808
    PRIDEiQ99808
    ProteomicsDBi78487

    Protocols and materials databases

    DNASUi2030
    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENST00000371708; ENSP00000360773; ENSG00000112759 [Q99808-1]
    ENST00000371713; ENSP00000360778; ENSG00000112759 [Q99808-1]
    ENST00000371724; ENSP00000360789; ENSG00000112759 [Q99808-1]
    ENST00000371755; ENSP00000360820; ENSG00000112759 [Q99808-1]
    ENST00000393844; ENSP00000377427; ENSG00000112759 [Q99808-1]
    ENST00000427851; ENSP00000392668; ENSG00000112759 [Q99808-1]
    GeneIDi2030
    KEGGihsa:2030
    UCSCiuc003owu.2 human [Q99808-1]

    Organism-specific databases

    CTDi2030
    DisGeNETi2030
    EuPathDBiHostDB:ENSG00000112759.16
    GeneCardsiSLC29A1
    HGNCiHGNC:11003 SLC29A1
    HPAiHPA012384
    MIMi602193 gene
    neXtProtiNX_Q99808
    OpenTargetsiENSG00000112759
    PharmGKBiPA154
    GenAtlasiSearch...

    Phylogenomic databases

    eggNOGiKOG1479 Eukaryota
    ENOG410Y3MT LUCA
    GeneTreeiENSGT00390000002232
    HOGENOMiHOG000007684
    HOVERGENiHBG074626
    InParanoidiQ99808
    KOiK15014
    OMAiMTPWNFF
    OrthoDBiEOG091G09WB
    PhylomeDBiQ99808
    TreeFamiTF313950

    Enzyme and pathway databases

    ReactomeiR-HSA-83936 Transport of nucleosides and free purine and pyrimidine bases across the plasma membrane

    Miscellaneous databases

    ChiTaRSiSLC29A1 human
    GeneWikiiEquilibrative_nucleoside_transporter_1
    GenomeRNAii2030
    PROiPR:Q99808
    SOURCEiSearch...

    Gene expression databases

    BgeeiENSG00000112759 Expressed in 190 organ(s), highest expression level in lower esophagus
    CleanExiHS_SLC29A1
    GenevisibleiQ99808 HS

    Family and domain databases

    InterProiView protein in InterPro
    IPR030195 ENT1
    IPR034764 ENT1/ENT2
    IPR002259 Eqnu_transpt
    IPR036259 MFS_trans_sf
    PANTHERiPTHR10332 PTHR10332, 1 hit
    PTHR10332:SF9 PTHR10332:SF9, 1 hit
    PfamiView protein in Pfam
    PF01733 Nucleoside_tran, 1 hit
    PIRSFiPIRSF016379 ENT, 1 hit
    PRINTSiPR01130 DERENTRNSPRT
    SUPFAMiSSF103473 SSF103473, 1 hit
    TIGRFAMsiTIGR00939 2a57, 1 hit
    ProtoNetiSearch...

    Entry informationi

    Entry nameiS29A1_HUMAN
    AccessioniPrimary (citable) accession number: Q99808
    Secondary accession number(s): B3KQV7
    , B3KQY5, Q5T9W9, Q9UJY2
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: January 23, 2007
    Last modified: November 7, 2018
    This is version 171 of the entry and version 3 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. Human chromosome 6
      Human chromosome 6: entries, gene names and cross-references to MIM
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