UniProtKB - Q997C2 (LAP_MYXVL)
Protein
E3 ubiquitin-protein ligase LAP
Gene
LAP
Organism
Myxoma virus (strain Lausanne) (MYXV)
Status
Functioni
E3 ubiquitin-protein ligase which promotes ubiquitination and subsequent degradation of host MHC-I and CD4 molecules, presumably to prevent lysis of infected cells by cytotoxic T-lymphocytes and NK cell. Binds target molecules through transmembrane interaction. The result of this ubiquitination is the enhancement of the endocytosis of the target chain and the delivery to the lysosome, where it is proteolytically destroyed (By similarity).By similarity2 Publications
Catalytic activityi
- S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine. EC:2.3.2.27
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 15 – 75 | RING-CH-typePROSITE-ProRule annotationAdd BLAST | 61 |
GO - Molecular functioni
- transferase activity Source: UniProtKB-KW
- zinc ion binding Source: InterPro
GO - Biological processi
- modulation by virus of host protein ubiquitination Source: UniProtKB-KW
- suppression by virus of host adaptive immune response Source: UniProtKB-KW
- suppression by virus of host antigen processing and presentation of peptide antigen via MHC class I Source: UniProtKB-KW
Keywordsi
Names & Taxonomyi
Protein namesi | Recommended name: E3 ubiquitin-protein ligase LAP (EC:2.3.2.27)Alternative name(s): Leukemia associated protein Short name: LAP RING-type E3 ubiquitin transferase LAPCurated |
Gene namesi | Name:LAP Ordered Locus Names:m153R |
Organismi | Myxoma virus (strain Lausanne) (MYXV) |
Taxonomic identifieri | 31530 [NCBI] |
Taxonomic lineagei | Viruses › Varidnaviria › Bamfordvirae › Nucleocytoviricota › Pokkesviricetes › Chitovirales › Poxviridae › Chordopoxvirinae › Leporipoxvirus › |
Virus hosti | Oryctolagus cuniculus (Rabbit) [TaxID: 9986] |
Proteomesi |
|
Subcellular locationi
- Host membrane Curated; Multi-pass membrane protein Curated
- host trans-Golgi network membrane 1 Publication
- Host early endosome membrane 1 Publication
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 93 | CytoplasmicSequence analysisAdd BLAST | 93 | |
Transmembranei | 94 – 114 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 115 – 129 | LumenalSequence analysisAdd BLAST | 15 | |
Transmembranei | 130 – 150 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 151 – 206 | CytoplasmicSequence analysisAdd BLAST | 56 |
Keywords - Cellular componenti
Host endosome, Host Golgi apparatus, Host membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000396001 | 1 – 206 | E3 ubiquitin-protein ligase LAPAdd BLAST | 206 |
Family & Domainsi
Domaini
The RING-CH-type zinc finger domain is required for E3 ligase activity.PROSITE-ProRule annotation
Sequence similaritiesi
Belongs to the poxviridae LAP protein family.Curated
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 15 – 75 | RING-CH-typePROSITE-ProRule annotationAdd BLAST | 61 |
Keywords - Domaini
Transmembrane, Transmembrane helix, Zinc-fingerFamily and domain databases
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR011016, Znf_RING-CH IPR013083, Znf_RING/FYVE/PHD |
Pfami | View protein in Pfam PF12906, RINGv, 1 hit |
SMARTi | View protein in SMART SM00744, RINGv, 1 hit |
PROSITEi | View protein in PROSITE PS51292, ZF_RING_CH, 1 hit |
i Sequence
Sequence statusi: Complete.
Q997C2-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MATVVNMDTV VNLDDVSLAD KCCWICKEAC DIVPNYCKCR GDNKIVHKEC
60 70 80 90 100
LEEWINTDVV KNKSCAICES PYNLKRRYKK ITKWRCYKRD CHDSLLVNMS
110 120 130 140 150
LCLIVGGMGG YLLISTEIVK LIASEEVSNI AKVFLVSASM GPFMVSALTM
160 170 180 190 200
VRACIDCRTY FIATRERNTI HEVAEMEDVE EVEEVNDDDG DEYVDAVEEI
VVESPA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF170726 Genomic DNA Translation: AAF15041.2 AF229033 Genomic DNA Translation: AAK00734.1 |
RefSeqi | NP_051866.2, NC_001132.2 |
Genome annotation databases
GeneIDi | 932099 |
KEGGi | vg:932099 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF170726 Genomic DNA Translation: AAF15041.2 AF229033 Genomic DNA Translation: AAK00734.1 |
RefSeqi | NP_051866.2, NC_001132.2 |
3D structure databases
SMRi | Q997C2 |
ModBasei | Search... |
Genome annotation databases
GeneIDi | 932099 |
KEGGi | vg:932099 |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR011016, Znf_RING-CH IPR013083, Znf_RING/FYVE/PHD |
Pfami | View protein in Pfam PF12906, RINGv, 1 hit |
SMARTi | View protein in SMART SM00744, RINGv, 1 hit |
PROSITEi | View protein in PROSITE PS51292, ZF_RING_CH, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | LAP_MYXVL | |
Accessioni | Q997C2Primary (citable) accession number: Q997C2 Secondary accession number(s): Q9Q8F2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 10, 2010 |
Last sequence update: | June 1, 2001 | |
Last modified: | August 12, 2020 | |
This is version 95 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families