UniProtKB - Q99788 (CML1_HUMAN)
Protein
Chemokine-like receptor 1
Gene
CMKLR1
Organism
Homo sapiens (Human)
Status
Functioni
Receptor for the chemoattractant adipokine chemerin/RARRES2 and for the omega-3 fatty acid derived molecule resolvin E1. Interaction with RARRES2 induces activation of intracellular signaling molecules, such as SKY, MAPK1/3 (ERK1/2), MAPK14/P38MAPK and PI3K leading to multifunctional effects, like, reduction of immune responses, enhancing of adipogenesis and angionesis. Resolvin E1 down-regulates cytokine production in macrophages by reducing the activation of MAPK1/3 (ERK1/2) and NF-kappa-B. Positively regulates adipogenesis and adipocyte metabolism. Acts as a coreceptor for several SIV strains (SIVMAC316, SIVMAC239, SIVMACL7E-FR and SIVSM62A), as well as a primary HIV-1 strain (92UG024-2).4 Publications
GO - Molecular functioni
- chemokine receptor activity Source: ProtInc
- complement receptor activity Source: GO_Central
- G protein-coupled receptor activity Source: GO_Central
- signaling receptor activity Source: ProtInc
GO - Biological processi
- chemotaxis Source: UniProtKB
- complement receptor mediated signaling pathway Source: GO_Central
- G protein-coupled receptor signaling pathway Source: UniProtKB
- immune response Source: ProtInc
- inflammatory response Source: GO_Central
- negative regulation of interleukin-12 production Source: UniProtKB
- negative regulation of NF-kappaB transcription factor activity Source: UniProtKB
- phospholipase C-activating G protein-coupled receptor signaling pathway Source: GO_Central
- positive regulation of cold-induced thermogenesis Source: YuBioLab
- positive regulation of cytosolic calcium ion concentration Source: GO_Central
- positive regulation of fat cell differentiation Source: UniProtKB
- positive regulation of macrophage chemotaxis Source: DFLAT
- regulation of calcium-mediated signaling Source: UniProtKB
- skeletal system development Source: ProtInc
Keywordsi
Molecular function | G-protein coupled receptor, Receptor, Transducer |
Enzyme and pathway databases
PathwayCommonsi | Q99788 |
Reactomei | R-HSA-373076, Class A/1 (Rhodopsin-like receptors) |
Protein family/group databases
TCDBi | 9.A.14.13.15, the g-protein-coupled receptor (gpcr) family |
Chemistry databases
SwissLipidsi | SLP:000001606 |
Names & Taxonomyi
Protein namesi | Recommended name: Chemokine-like receptor 1Alternative name(s): G-protein coupled receptor ChemR23 G-protein coupled receptor DEZ |
Gene namesi | Name:CMKLR1 Synonyms:CHEMR23, DEZ |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:2121, CMKLR1 |
MIMi | 602351, gene |
neXtProti | NX_Q99788 |
VEuPathDBi | HostDB:ENSG00000174600.13 |
Subcellular locationi
Plasma membrane
- Cell membrane 2 Publications; Multi-pass membrane protein 2 Publications
Plasma Membrane
- integral component of plasma membrane Source: ProtInc
- plasma membrane Source: UniProtKB
Other locations
- integral component of membrane Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 41 | ExtracellularSequence analysisAdd BLAST | 41 | |
Transmembranei | 42 – 64 | Helical; Name=1Sequence analysisAdd BLAST | 23 | |
Topological domaini | 65 – 75 | CytoplasmicSequence analysisAdd BLAST | 11 | |
Transmembranei | 76 – 97 | Helical; Name=2Sequence analysisAdd BLAST | 22 | |
Topological domaini | 98 – 114 | ExtracellularSequence analysisAdd BLAST | 17 | |
Transmembranei | 115 – 135 | Helical; Name=3Sequence analysisAdd BLAST | 21 | |
Topological domaini | 136 – 154 | CytoplasmicSequence analysisAdd BLAST | 19 | |
Transmembranei | 155 – 176 | Helical; Name=4Sequence analysisAdd BLAST | 22 | |
Topological domaini | 177 – 224 | ExtracellularSequence analysisAdd BLAST | 48 | |
Transmembranei | 225 – 245 | Helical; Name=5Sequence analysisAdd BLAST | 21 | |
Topological domaini | 246 – 261 | CytoplasmicSequence analysisAdd BLAST | 16 | |
Transmembranei | 262 – 282 | Helical; Name=6Sequence analysisAdd BLAST | 21 | |
Topological domaini | 283 – 300 | ExtracellularSequence analysisAdd BLAST | 18 | |
Transmembranei | 301 – 320 | Helical; Name=7Sequence analysisAdd BLAST | 20 | |
Topological domaini | 321 – 373 | CytoplasmicSequence analysisAdd BLAST | 53 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 1240 |
OpenTargetsi | ENSG00000174600 |
PharmGKBi | PA26640 |
Miscellaneous databases
Pharosi | Q99788, Tchem |
Chemistry databases
ChEMBLi | CHEMBL3540 |
GuidetoPHARMACOLOGYi | 79 |
Genetic variation databases
BioMutai | CMKLR1 |
DMDMi | 17380487 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000069307 | 1 – 373 | Chemokine-like receptor 1Add BLAST | 373 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 9 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 112 ↔ 189 | PROSITE-ProRule annotation | ||
Glycosylationi | 192 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 339 | PhosphoserineBy similarity | 1 | |
Modified residuei | 342 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 349 | PhosphoserineBy similarity | 1 | |
Modified residuei | 352 | PhosphoserineBy similarity | 1 | |
Modified residuei | 358 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Disulfide bond, Glycoprotein, PhosphoproteinProteomic databases
jPOSTi | Q99788 |
PaxDbi | Q99788 |
PeptideAtlasi | Q99788 |
PRIDEi | Q99788 |
ProteomicsDBi | 78474 [Q99788-1] 78475 [Q99788-2] |
PTM databases
GlyGeni | Q99788, 2 sites |
iPTMneti | Q99788 |
PhosphoSitePlusi | Q99788 |
Expressioni
Tissue specificityi
Prominently expressed in developing osseous and cartilaginous tissue. Also found in adult parathyroid glands. Expressed in cardiovascular system, brain, kidney, gastrointestinal tissues and myeloid tissues. Expressed in a broad array of tissues associated with hematopoietic and immune function including, spleen, thymus, appendix, lymph node, bone marrow and fetal liver. Among leukocyte populations abundant expression in monocyte-derived macrophage and immature dendritic cells (DCs). High expression in blood monocytes and low levels in polymorphonuclear cells and T-cells. Expressed on endothelial cells. Highly expressed in differentiating adipocytes.6 Publications
Developmental stagei
Expressed during bone development.
Inductioni
Up-regulated by inflammatory cytokines TNF-alpha and IFN-gamma in monocytes. Up-regulated by TNF-alpha, IL-1-beta and IL-6 in endothelial cells.3 Publications
Gene expression databases
Bgeei | ENSG00000174600, Expressed in tendon and 157 other tissues |
ExpressionAtlasi | Q99788, baseline and differential |
Genevisiblei | Q99788, HS |
Organism-specific databases
HPAi | ENSG00000174600, Tissue enhanced (lymphoid) |
Interactioni
Protein-protein interaction databases
BioGRIDi | 107644, 79 interactors |
STRINGi | 9606.ENSP00000311733 |
Chemistry databases
BindingDBi | Q99788 |
Miscellaneous databases
RNActi | Q99788, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3656, Eukaryota |
GeneTreei | ENSGT01010000222468 |
HOGENOMi | CLU_009579_8_0_1 |
InParanoidi | Q99788 |
OMAi | NIAYTAM |
OrthoDBi | 910274at2759 |
PhylomeDBi | Q99788 |
TreeFami | TF330976 |
Family and domain databases
InterProi | View protein in InterPro IPR002258, Chemokine-like_recpt_1 IPR000826, Formyl_rcpt-rel IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM |
PANTHERi | PTHR24225, PTHR24225, 1 hit PTHR24225:SF49, PTHR24225:SF49, 1 hit |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR01126, DEZORPHANR PR00237, GPCRRHODOPSN |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
Isoform A (identifier: Q99788-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MRMEDEDYNT SISYGDEYPD YLDSIVVLED LSPLEARVTR IFLVVVYSIV
60 70 80 90 100
CFLGILGNGL VIIIATFKMK KTVNMVWFLN LAVADFLFNV FLPIHITYAA
110 120 130 140 150
MDYHWVFGTA MCKISNFLLI HNMFTSVFLL TIISSDRCIS VLLPVWSQNH
160 170 180 190 200
RSVRLAYMAC MVIWVLAFFL SSPSLVFRDT ANLHGKISCF NNFSLSTPGS
210 220 230 240 250
SSWPTHSQMD PVGYSRHMVV TVTRFLCGFL VPVLIITACY LTIVCKLQRN
260 270 280 290 300
RLAKTKKPFK IIVTIIITFF LCWCPYHTLN LLELHHTAMP GSVFSLGLPL
310 320 330 340 350
ATALAIANSC MNPILYVFMG QDFKKFKVAL FSRLVNALSE DTGHSSYPSH
360 370
RSFTKMSSMN ERTSMNERET GML
Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF8VYN7 | F8VYN7_HUMAN | Chemokine-like receptor 1 | CMKLR1 | 147 | Annotation score: | ||
F8VSC8 | F8VSC8_HUMAN | Chemokine-like receptor 1 | CMKLR1 | 143 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 248 | Q → H in AAC51258 (PubMed:9603476).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_001985 | 1 – 2 | Missing in isoform B. 3 Publications | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U79526 mRNA Translation: AAC51258.1 U79527 mRNA Translation: AAC51259.1 Y14838 Genomic DNA Translation: CAA75112.1 AB065871 Genomic DNA Translation: BAC06089.1 AY497547 mRNA Translation: AAR90850.1 AK291800 mRNA Translation: BAF84489.1 BT019556 mRNA Translation: AAV38363.1 BT019557 mRNA Translation: AAV38364.1 AC009729 Genomic DNA No translation available. AC063957 Genomic DNA No translation available. CH471054 Genomic DNA Translation: EAW97810.1 CH471054 Genomic DNA Translation: EAW97811.1 BC106927 mRNA Translation: AAI06928.1 BC106928 mRNA Translation: AAI06929.1 |
CCDSi | CCDS41829.1 [Q99788-2] CCDS44965.1 [Q99788-1] |
RefSeqi | NP_001135815.1, NM_001142343.1 [Q99788-1] NP_001135816.1, NM_001142344.1 [Q99788-1] NP_001135817.1, NM_001142345.1 [Q99788-1] NP_004063.1, NM_004072.2 [Q99788-2] XP_016874309.1, XM_017018820.1 [Q99788-2] |
Genome annotation databases
Ensembli | ENST00000312143; ENSP00000311733; ENSG00000174600 [Q99788-1] ENST00000412676; ENSP00000401293; ENSG00000174600 [Q99788-1] ENST00000550402; ENSP00000449716; ENSG00000174600 [Q99788-1] ENST00000552995; ENSP00000447579; ENSG00000174600 [Q99788-2] |
GeneIDi | 1240 |
KEGGi | hsa:1240 |
UCSCi | uc001tmv.4, human [Q99788-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U79526 mRNA Translation: AAC51258.1 U79527 mRNA Translation: AAC51259.1 Y14838 Genomic DNA Translation: CAA75112.1 AB065871 Genomic DNA Translation: BAC06089.1 AY497547 mRNA Translation: AAR90850.1 AK291800 mRNA Translation: BAF84489.1 BT019556 mRNA Translation: AAV38363.1 BT019557 mRNA Translation: AAV38364.1 AC009729 Genomic DNA No translation available. AC063957 Genomic DNA No translation available. CH471054 Genomic DNA Translation: EAW97810.1 CH471054 Genomic DNA Translation: EAW97811.1 BC106927 mRNA Translation: AAI06928.1 BC106928 mRNA Translation: AAI06929.1 |
CCDSi | CCDS41829.1 [Q99788-2] CCDS44965.1 [Q99788-1] |
RefSeqi | NP_001135815.1, NM_001142343.1 [Q99788-1] NP_001135816.1, NM_001142344.1 [Q99788-1] NP_001135817.1, NM_001142345.1 [Q99788-1] NP_004063.1, NM_004072.2 [Q99788-2] XP_016874309.1, XM_017018820.1 [Q99788-2] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 107644, 79 interactors |
STRINGi | 9606.ENSP00000311733 |
Chemistry databases
BindingDBi | Q99788 |
ChEMBLi | CHEMBL3540 |
GuidetoPHARMACOLOGYi | 79 |
SwissLipidsi | SLP:000001606 |
Protein family/group databases
TCDBi | 9.A.14.13.15, the g-protein-coupled receptor (gpcr) family |
GPCRDBi | Search... |
PTM databases
GlyGeni | Q99788, 2 sites |
iPTMneti | Q99788 |
PhosphoSitePlusi | Q99788 |
Genetic variation databases
BioMutai | CMKLR1 |
DMDMi | 17380487 |
Proteomic databases
jPOSTi | Q99788 |
PaxDbi | Q99788 |
PeptideAtlasi | Q99788 |
PRIDEi | Q99788 |
ProteomicsDBi | 78474 [Q99788-1] 78475 [Q99788-2] |
Protocols and materials databases
Antibodypediai | 9299, 563 antibodies |
DNASUi | 1240 |
Genome annotation databases
Ensembli | ENST00000312143; ENSP00000311733; ENSG00000174600 [Q99788-1] ENST00000412676; ENSP00000401293; ENSG00000174600 [Q99788-1] ENST00000550402; ENSP00000449716; ENSG00000174600 [Q99788-1] ENST00000552995; ENSP00000447579; ENSG00000174600 [Q99788-2] |
GeneIDi | 1240 |
KEGGi | hsa:1240 |
UCSCi | uc001tmv.4, human [Q99788-1] |
Organism-specific databases
CTDi | 1240 |
DisGeNETi | 1240 |
GeneCardsi | CMKLR1 |
HGNCi | HGNC:2121, CMKLR1 |
HPAi | ENSG00000174600, Tissue enhanced (lymphoid) |
MIMi | 602351, gene |
neXtProti | NX_Q99788 |
OpenTargetsi | ENSG00000174600 |
PharmGKBi | PA26640 |
VEuPathDBi | HostDB:ENSG00000174600.13 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3656, Eukaryota |
GeneTreei | ENSGT01010000222468 |
HOGENOMi | CLU_009579_8_0_1 |
InParanoidi | Q99788 |
OMAi | NIAYTAM |
OrthoDBi | 910274at2759 |
PhylomeDBi | Q99788 |
TreeFami | TF330976 |
Enzyme and pathway databases
PathwayCommonsi | Q99788 |
Reactomei | R-HSA-373076, Class A/1 (Rhodopsin-like receptors) |
Miscellaneous databases
BioGRID-ORCSi | 1240, 91 hits in 866 CRISPR screens |
ChiTaRSi | CMKLR1, human |
GeneWikii | CMKLR1 |
GenomeRNAii | 1240 |
Pharosi | Q99788, Tchem |
PROi | PR:Q99788 |
RNActi | Q99788, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000174600, Expressed in tendon and 157 other tissues |
ExpressionAtlasi | Q99788, baseline and differential |
Genevisiblei | Q99788, HS |
Family and domain databases
InterProi | View protein in InterPro IPR002258, Chemokine-like_recpt_1 IPR000826, Formyl_rcpt-rel IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM |
PANTHERi | PTHR24225, PTHR24225, 1 hit PTHR24225:SF49, PTHR24225:SF49, 1 hit |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR01126, DEZORPHANR PR00237, GPCRRHODOPSN |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CML1_HUMAN | |
Accessioni | Q99788Primary (citable) accession number: Q99788 Secondary accession number(s): A8K6Y5 Q99789 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | November 16, 2001 | |
Last modified: | February 10, 2021 | |
This is version 178 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 12
Human chromosome 12: entries, gene names and cross-references to MIM - 7-transmembrane G-linked receptors
List of 7-transmembrane G-linked receptor entries - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families