UniProtKB - Q99750 (MDFI_HUMAN)
MyoD family inhibitor
MDFI
Functioni
Inhibits the transactivation activity of the Myod family of myogenic factors and represses myogenesis. Acts by associating with Myod family members and retaining them in the cytoplasm by masking their nuclear localization signals. Can also interfere with the DNA-binding activity of Myod family members. Plays an important role in trophoblast and chondrogenic differentiation. Regulates the transcriptional activity of TCF7L1/TCF3 by interacting directly with TCF7L1/TCF3 and preventing it from binding DNA. Binds to the axin complex, resulting in an increase in the level of free beta-catenin. Affects axin regulation of the WNT and JNK signaling pathways (By similarity).
By similarityGO - Molecular functioni
- identical protein binding Source: IntAct
- transcription regulator inhibitor activity Source: GO_Central
GO - Biological processi
- cytoplasmic sequestering of transcription factor Source: ProtInc
- dorsal/ventral axis specification Source: UniProtKB
- embryonic skeletal system morphogenesis Source: Ensembl
- negative regulation of DNA binding Source: UniProtKB
- negative regulation of transcription by RNA polymerase II Source: UniProtKB
- negative regulation of Wnt signaling pathway Source: UniProtKB
- trophoblast giant cell differentiation Source: Ensembl
Keywordsi
Molecular function | Developmental protein |
Biological process | Differentiation |
Enzyme and pathway databases
PathwayCommonsi | Q99750 |
SignaLinki | Q99750 |
SIGNORi | Q99750 |
Names & Taxonomyi
Protein namesi | Recommended name: MyoD family inhibitorAlternative name(s): Myogenic repressor I-mf |
Gene namesi | Name:MDFI |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:6967, MDFI |
MIMi | 604971, gene |
neXtProti | NX_Q99750 |
VEuPathDBi | HostDB:ENSG00000112559 |
Pathology & Biotechi
Organism-specific databases
DisGeNETi | 4188 |
OpenTargetsi | ENSG00000112559 |
PharmGKBi | PA30713 |
Miscellaneous databases
Pharosi | Q99750, Tbio |
Genetic variation databases
BioMutai | MDFI |
DMDMi | 25090674 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000096324 | 1 – 246 | MyoD family inhibitorAdd BLAST | 246 |
Proteomic databases
MassIVEi | Q99750 |
MaxQBi | Q99750 |
PaxDbi | Q99750 |
PeptideAtlasi | Q99750 |
PRIDEi | Q99750 |
ProteomicsDBi | 78457 |
PTM databases
iPTMneti | Q99750 |
PhosphoSitePlusi | Q99750 |
Expressioni
Gene expression databases
Bgeei | ENSG00000112559, Expressed in stomach and 175 other tissues |
ExpressionAtlasi | Q99750, baseline and differential |
Genevisiblei | Q99750, HS |
Organism-specific databases
HPAi | ENSG00000112559, Tissue enhanced (esophagus) |
Interactioni
Subunit structurei
The C-terminus interacts with AXIN1 and LEF1 (By similarity).
Interacts with CCNT2 (PubMed:17289077).
By similarity1 PublicationBinary interactionsi
Q99750
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 110353, 518 interactors |
IntActi | Q99750, 409 interactors |
MINTi | Q99750 |
STRINGi | 9606.ENSP00000230321 |
Miscellaneous databases
RNActi | Q99750, protein |
Structurei
3D structure databases
AlphaFoldDBi | Q99750 |
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 99 – 246 | MDFIAdd BLAST | 148 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 76 | DisorderedSequence analysisAdd BLAST | 76 | |
Regioni | 91 – 151 | DisorderedSequence analysisAdd BLAST | 61 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 132 – 151 | Polar residuesSequence analysisAdd BLAST | 20 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | ENOG502RZMC, Eukaryota |
GeneTreei | ENSGT00940000160187 |
HOGENOMi | CLU_067479_1_0_1 |
InParanoidi | Q99750 |
OMAi | CAECDLP |
OrthoDBi | 1487193at2759 |
PhylomeDBi | Q99750 |
TreeFami | TF332113 |
Family and domain databases
InterProi | View protein in InterPro IPR026134, MDFI/MDFIC |
PANTHERi | PTHR15304, PTHR15304, 1 hit |
Pfami | View protein in Pfam PF15316, MDFI, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 6 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MYQVSGQRPS GCDAPYGAPS AAPGPAQTLS LLPGLEVVTG STHPAEAAPE
60 70 80 90 100
EGSLEEAATP MPQGNGPGIP QGLDSTDLDV PTEAVTCQPQ GNPLGCTPLL
110 120 130 140 150
PNDSGHPSEL GGTRRAGNGA LGGPKAHRKL QTHPSLASQG SKKSKSSSKS
160 170 180 190 200
TTSQIPLQAQ EDCCVHCILS CLFCEFLTLC NIVLDCATCG SCSSEDSCLC
210 220 230 240
CCCCGSGECA DCDLPCDLDC GILDACCESA DCLEICMECC GLCFSS
Computationally mapped potential isoform sequencesi
There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketB1AKB6 | B1AKB6_HUMAN | MyoD family inhibitor | MDFI | 185 | Annotation score: | ||
B1AKB9 | B1AKB9_HUMAN | MyoD family inhibitor | MDFI | 142 | Annotation score: | ||
B1AKB7 | B1AKB7_HUMAN | MyoD family inhibitor | MDFI | 231 | Annotation score: | ||
B1AKB8 | B1AKB8_HUMAN | MyoD family inhibitor | MDFI | 213 | Annotation score: | ||
B1AKC0 | B1AKC0_HUMAN | MyoD family inhibitor | MDFI | 128 | Annotation score: | ||
B1AKC1 | B1AKC1_HUMAN | MyoD family inhibitor | MDFI | 98 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U78313 mRNA Translation: AAB39748.1 AL035588 Genomic DNA No translation available. BC007836 mRNA Translation: AAH07836.1 |
CCDSi | CCDS4857.1 |
RefSeqi | NP_001287733.1, NM_001300804.1 NP_001287734.1, NM_001300805.1 NP_001287735.1, NM_001300806.1 NP_005577.1, NM_005586.3 |
Genome annotation databases
Ensembli | ENST00000230321.11; ENSP00000230321.6; ENSG00000112559.15 ENST00000373051.6; ENSP00000362142.2; ENSG00000112559.15 |
GeneIDi | 4188 |
KEGGi | hsa:4188 |
MANE-Selecti | ENST00000230321.11; ENSP00000230321.6; NM_005586.4; NP_005577.1 |
UCSCi | uc003oqq.5, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U78313 mRNA Translation: AAB39748.1 AL035588 Genomic DNA No translation available. BC007836 mRNA Translation: AAH07836.1 |
CCDSi | CCDS4857.1 |
RefSeqi | NP_001287733.1, NM_001300804.1 NP_001287734.1, NM_001300805.1 NP_001287735.1, NM_001300806.1 NP_005577.1, NM_005586.3 |
3D structure databases
AlphaFoldDBi | Q99750 |
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 110353, 518 interactors |
IntActi | Q99750, 409 interactors |
MINTi | Q99750 |
STRINGi | 9606.ENSP00000230321 |
PTM databases
iPTMneti | Q99750 |
PhosphoSitePlusi | Q99750 |
Genetic variation databases
BioMutai | MDFI |
DMDMi | 25090674 |
Proteomic databases
MassIVEi | Q99750 |
MaxQBi | Q99750 |
PaxDbi | Q99750 |
PeptideAtlasi | Q99750 |
PRIDEi | Q99750 |
ProteomicsDBi | 78457 |
Protocols and materials databases
Antibodypediai | 30051, 166 antibodies from 29 providers |
DNASUi | 4188 |
Genome annotation databases
Ensembli | ENST00000230321.11; ENSP00000230321.6; ENSG00000112559.15 ENST00000373051.6; ENSP00000362142.2; ENSG00000112559.15 |
GeneIDi | 4188 |
KEGGi | hsa:4188 |
MANE-Selecti | ENST00000230321.11; ENSP00000230321.6; NM_005586.4; NP_005577.1 |
UCSCi | uc003oqq.5, human |
Organism-specific databases
CTDi | 4188 |
DisGeNETi | 4188 |
GeneCardsi | MDFI |
HGNCi | HGNC:6967, MDFI |
HPAi | ENSG00000112559, Tissue enhanced (esophagus) |
MIMi | 604971, gene |
neXtProti | NX_Q99750 |
OpenTargetsi | ENSG00000112559 |
PharmGKBi | PA30713 |
VEuPathDBi | HostDB:ENSG00000112559 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502RZMC, Eukaryota |
GeneTreei | ENSGT00940000160187 |
HOGENOMi | CLU_067479_1_0_1 |
InParanoidi | Q99750 |
OMAi | CAECDLP |
OrthoDBi | 1487193at2759 |
PhylomeDBi | Q99750 |
TreeFami | TF332113 |
Enzyme and pathway databases
PathwayCommonsi | Q99750 |
SignaLinki | Q99750 |
SIGNORi | Q99750 |
Miscellaneous databases
BioGRID-ORCSi | 4188, 8 hits in 1046 CRISPR screens |
ChiTaRSi | MDFI, human |
GeneWikii | MDFI |
GenomeRNAii | 4188 |
Pharosi | Q99750, Tbio |
PROi | PR:Q99750 |
RNActi | Q99750, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000112559, Expressed in stomach and 175 other tissues |
ExpressionAtlasi | Q99750, baseline and differential |
Genevisiblei | Q99750, HS |
Family and domain databases
InterProi | View protein in InterPro IPR026134, MDFI/MDFIC |
PANTHERi | PTHR15304, PTHR15304, 1 hit |
Pfami | View protein in Pfam PF15316, MDFI, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MDFI_HUMAN | |
Accessioni | Q99750Primary (citable) accession number: Q99750 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 15, 2002 |
Last sequence update: | May 1, 1997 | |
Last modified: | May 25, 2022 | |
This is version 167 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 6
Human chromosome 6: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families