UniProtKB - Q99733 (NP1L4_HUMAN)
Protein
Nucleosome assembly protein 1-like 4
Gene
NAP1L4
Organism
Homo sapiens (Human)
Status
Functioni
Acts as histone chaperone in nucleosome assembly.1 Publication
GO - Molecular functioni
- chromatin binding Source: GO_Central
- histone binding Source: GO_Central
- nucleosome binding Source: UniProtKB
- RNA binding Source: UniProtKB
- unfolded protein binding Source: ProtInc
GO - Biological processi
- nucleosome assembly Source: UniProtKB
Keywordsi
Molecular function | Chaperone |
Enzyme and pathway databases
PathwayCommonsi | Q99733 |
Names & Taxonomyi
Protein namesi | Recommended name: Nucleosome assembly protein 1-like 4Alternative name(s): Nucleosome assembly protein 21 Publication Short name: NAP-21 Publication |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:7640, NAP1L4 |
MIMi | 601651, gene |
neXtProti | NX_Q99733 |
VEuPathDBi | HostDB:ENSG00000205531.12 |
Subcellular locationi
Nucleus
- Nucleus 2 Publications
Cytoplasm and Cytosol
- Cytoplasm 2 Publications
Note: Present in the cytoplasm and excluded from the nucleus during G0/G1 phase, then relocates to the nucleus by the time cells are in S phase (PubMed:9325046). Phosphorylated form localizes in the cytoplasm during the G0/G1 transition, whereas dephosphorylation leads to relocalization into the nucleus at the G1/S-boundary (PubMed:10764593).2 Publications
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 4676 |
OpenTargetsi | ENSG00000205531 |
PharmGKBi | PA31442 |
Miscellaneous databases
Pharosi | Q99733, Tbio |
Chemistry databases
ChEMBLi | CHEMBL3886061 |
Genetic variation databases
BioMutai | NAP1L4 |
DMDMi | 2498672 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000185658 | 2 – 375 | Nucleosome assembly protein 1-like 4Add BLAST | 374 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources | 1 | |
Modified residuei | 5 | PhosphoserineCombined sources | 1 | |
Modified residuei | 7 | PhosphoserineCombined sources | 1 | |
Modified residuei | 12 | PhosphoserineCombined sources | 1 | |
Modified residuei | 51 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 53 | PhosphoserineBy similarity | 1 | |
Modified residuei | 54 | PhosphoserineCombined sources | 1 | |
Modified residuei | 58 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 105 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 125 | PhosphoserineCombined sources | 1 | |
Modified residuei | 146 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 304 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Phosphorylated at the G0/G1 boundary but it is not phosphorylated in S-phase. Phosphorylated protein remains in the cytoplasm in a complex with histones during the G0/G1 transition, whereas dephosphorylation triggers its transport into the nucleus at the G1/S-boundary.1 Publication
Polyglutamylated by TTLL4, a modification that occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Some residues may also be monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human (By similarity).By similarityCurated
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q99733 |
jPOSTi | Q99733 |
MassIVEi | Q99733 |
MaxQBi | Q99733 |
PaxDbi | Q99733 |
PeptideAtlasi | Q99733 |
PRIDEi | Q99733 |
ProteomicsDBi | 27835 78450 [Q99733-1] |
PTM databases
iPTMneti | Q99733 |
MetOSitei | Q99733 |
PhosphoSitePlusi | Q99733 |
SwissPalmi | Q99733 |
Expressioni
Tissue specificityi
Ubiquitous. Biallelically expressed in fetal and adult tissues. Highest levels in testis.1 Publication
Gene expression databases
Bgeei | ENSG00000205531, Expressed in testis and 243 other tissues |
ExpressionAtlasi | Q99733, baseline and differential |
Genevisiblei | Q99733, HS |
Organism-specific databases
HPAi | ENSG00000205531, Low tissue specificity |
Interactioni
Subunit structurei
Interacts with core (H2A, H2B, H3, H4) and linker (H1) histones.
1 PublicationBinary interactionsi
Hide detailsQ99733
With | #Exp. | IntAct |
---|---|---|
NAP1L1 [P55209] | 2 | EBI-2255116,EBI-356392 |
tat [P04608] from Human immunodeficiency virus type 1 group M subtype B (isolate HXB2). | 3 | EBI-2255116,EBI-6164389 |
GO - Molecular functioni
- histone binding Source: GO_Central
- unfolded protein binding Source: ProtInc
Protein-protein interaction databases
BioGRIDi | 110757, 125 interactors |
IntActi | Q99733, 60 interactors |
MINTi | Q99733 |
STRINGi | 9606.ENSP00000369915 |
Miscellaneous databases
RNActi | Q99733, protein |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 265 – 271 | Nuclear localization signalSequence analysis | 7 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 126 – 131 | Poly-Glu | 6 | |
Compositional biasi | 301 – 310 | Asp/Glu-rich (acidic) | 10 | |
Compositional biasi | 347 – 370 | Asp/Glu-rich (acidic)Add BLAST | 24 |
Sequence similaritiesi
Belongs to the nucleosome assembly protein (NAP) family.Curated
Phylogenomic databases
eggNOGi | KOG1507, Eukaryota |
GeneTreei | ENSGT00940000153362 |
HOGENOMi | CLU_038841_3_0_1 |
InParanoidi | Q99733 |
OMAi | EYDEDHG |
PhylomeDBi | Q99733 |
TreeFami | TF314349 |
Family and domain databases
InterProi | View protein in InterPro IPR037231, NAP-like_sf IPR002164, NAP_family |
PANTHERi | PTHR11875, PTHR11875, 1 hit |
Pfami | View protein in Pfam PF00956, NAP, 1 hit |
SUPFAMi | SSF143113, SSF143113, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 12 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q99733-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MADHSFSDGV PSDSVEAAKN ASNTEKLTDQ VMQNPRVLAA LQERLDNVPH
60 70 80 90 100
TPSSYIETLP KAVKRRINAL KQLQVRCAHI EAKFYEEVHD LERKYAALYQ
110 120 130 140 150
PLFDKRREFI TGDVEPTDAE SEWHSENEEE EKLAGDMKSK VVVTEKAAAT
160 170 180 190 200
AEEPDPKGIP EFWFTIFRNV DMLSELVQEY DEPILKHLQD IKVKFSDPGQ
210 220 230 240 250
PMSFVLEFHF EPNDYFTNSV LTKTYKMKSE PDKADPFSFE GPEIVDCDGC
260 270 280 290 300
TIDWKKGKNV TVKTIKKKQK HKGRGTVRTI TKQVPNESFF NFFNPLKASG
310 320 330 340 350
DGESLDEDSE FTLASDFEIG HFFRERIVPR AVLYFTGEAI EDDDNFEEGE
360 370
EGEEEELEGD EEGEDEDDAE INPKV
Computationally mapped potential isoform sequencesi
There are 12 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9PP22 | E9PP22_HUMAN | Nucleosome assembly protein 1-like ... | NAP1L4 | 154 | Annotation score: | ||
E9PS34 | E9PS34_HUMAN | Nucleosome assembly protein 1-like ... | NAP1L4 | 133 | Annotation score: | ||
C9J6D1 | C9J6D1_HUMAN | Nucleosome assembly protein 1-like ... | NAP1L4 | 178 | Annotation score: | ||
E9PNW0 | E9PNW0_HUMAN | Nucleosome assembly protein 1-like ... | NAP1L4 | 107 | Annotation score: | ||
E9PJJ2 | E9PJJ2_HUMAN | Nucleosome assembly protein 1-like ... | NAP1L4 | 128 | Annotation score: | ||
E9PKT8 | E9PKT8_HUMAN | Nucleosome assembly protein 1-like ... | NAP1L4 | 169 | Annotation score: | ||
E9PNJ7 | E9PNJ7_HUMAN | Nucleosome assembly protein 1-like ... | NAP1L4 | 140 | Annotation score: | ||
C9J1B1 | C9J1B1_HUMAN | Nucleosome assembly protein 1-like ... | NAP1L4 | 101 | Annotation score: | ||
E9PKI2 | E9PKI2_HUMAN | Nucleosome assembly protein 1-like ... | NAP1L4 | 78 | Annotation score: | ||
A8MXH2 | A8MXH2_HUMAN | Nucleosome assembly protein 1-like ... | NAP1L4 | 156 | Annotation score: | ||
There are more potential isoformsShow all |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 107 | R → K in BAG35491 (PubMed:14702039).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_053910 | 375 | V → KEPSQPAECKQQ in isoform 2. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U77456 mRNA Translation: AAC50870.1 AB500094 mRNA Translation: BAH95827.1 AK312599 mRNA Translation: BAG35491.1 AC131971 Genomic DNA No translation available. BC022090 mRNA Translation: AAH22090.1 |
CCDSi | CCDS41599.1 [Q99733-1] |
RefSeqi | NP_005960.1, NM_005969.3 [Q99733-1] |
Genome annotation databases
Ensembli | ENST00000380542; ENSP00000369915; ENSG00000205531 [Q99733-1] ENST00000526115; ENSP00000436397; ENSG00000205531 [Q99733-2] ENST00000616696; ENSP00000478654; ENSG00000273562 [Q99733-2] ENST00000619695; ENSP00000483728; ENSG00000273562 [Q99733-1] ENST00000620138; ENSP00000481412; ENSG00000205531 [Q99733-2] ENST00000632791; ENSP00000487984; ENSG00000273562 [Q99733-2] |
GeneIDi | 4676 |
KEGGi | hsa:4676 |
UCSCi | uc001lxc.4, human [Q99733-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U77456 mRNA Translation: AAC50870.1 AB500094 mRNA Translation: BAH95827.1 AK312599 mRNA Translation: BAG35491.1 AC131971 Genomic DNA No translation available. BC022090 mRNA Translation: AAH22090.1 |
CCDSi | CCDS41599.1 [Q99733-1] |
RefSeqi | NP_005960.1, NM_005969.3 [Q99733-1] |
3D structure databases
SMRi | Q99733 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 110757, 125 interactors |
IntActi | Q99733, 60 interactors |
MINTi | Q99733 |
STRINGi | 9606.ENSP00000369915 |
Chemistry databases
ChEMBLi | CHEMBL3886061 |
PTM databases
iPTMneti | Q99733 |
MetOSitei | Q99733 |
PhosphoSitePlusi | Q99733 |
SwissPalmi | Q99733 |
Genetic variation databases
BioMutai | NAP1L4 |
DMDMi | 2498672 |
Proteomic databases
EPDi | Q99733 |
jPOSTi | Q99733 |
MassIVEi | Q99733 |
MaxQBi | Q99733 |
PaxDbi | Q99733 |
PeptideAtlasi | Q99733 |
PRIDEi | Q99733 |
ProteomicsDBi | 27835 78450 [Q99733-1] |
Protocols and materials databases
Antibodypediai | 23223, 72 antibodies |
DNASUi | 4676 |
Genome annotation databases
Ensembli | ENST00000380542; ENSP00000369915; ENSG00000205531 [Q99733-1] ENST00000526115; ENSP00000436397; ENSG00000205531 [Q99733-2] ENST00000616696; ENSP00000478654; ENSG00000273562 [Q99733-2] ENST00000619695; ENSP00000483728; ENSG00000273562 [Q99733-1] ENST00000620138; ENSP00000481412; ENSG00000205531 [Q99733-2] ENST00000632791; ENSP00000487984; ENSG00000273562 [Q99733-2] |
GeneIDi | 4676 |
KEGGi | hsa:4676 |
UCSCi | uc001lxc.4, human [Q99733-1] |
Organism-specific databases
CTDi | 4676 |
DisGeNETi | 4676 |
GeneCardsi | NAP1L4 |
HGNCi | HGNC:7640, NAP1L4 |
HPAi | ENSG00000205531, Low tissue specificity |
MIMi | 601651, gene |
neXtProti | NX_Q99733 |
OpenTargetsi | ENSG00000205531 |
PharmGKBi | PA31442 |
VEuPathDBi | HostDB:ENSG00000205531.12 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1507, Eukaryota |
GeneTreei | ENSGT00940000153362 |
HOGENOMi | CLU_038841_3_0_1 |
InParanoidi | Q99733 |
OMAi | EYDEDHG |
PhylomeDBi | Q99733 |
TreeFami | TF314349 |
Enzyme and pathway databases
PathwayCommonsi | Q99733 |
Miscellaneous databases
BioGRID-ORCSi | 4676, 5 hits in 999 CRISPR screens |
ChiTaRSi | NAP1L4, human |
GeneWikii | NAP1L4 |
GenomeRNAii | 4676 |
Pharosi | Q99733, Tbio |
PROi | PR:Q99733 |
RNActi | Q99733, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000205531, Expressed in testis and 243 other tissues |
ExpressionAtlasi | Q99733, baseline and differential |
Genevisiblei | Q99733, HS |
Family and domain databases
InterProi | View protein in InterPro IPR037231, NAP-like_sf IPR002164, NAP_family |
PANTHERi | PTHR11875, PTHR11875, 1 hit |
Pfami | View protein in Pfam PF00956, NAP, 1 hit |
SUPFAMi | SSF143113, SSF143113, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NP1L4_HUMAN | |
Accessioni | Q99733Primary (citable) accession number: Q99733 Secondary accession number(s): B2R6J4, F5HFY4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | May 1, 1997 | |
Last modified: | April 7, 2021 | |
This is version 178 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 11
Human chromosome 11: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families