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Protein

Mothers against decapentaplegic homolog 5

Gene

SMAD5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi65ZincBy similarity1
Metal bindingi110ZincBy similarity1
Metal bindingi122ZincBy similarity1
Metal bindingi127ZincBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-201451 Signaling by BMP
SignaLinkiQ99717
SIGNORiQ99717

Names & Taxonomyi

Protein namesi
Recommended name:
Mothers against decapentaplegic homolog 5
Short name:
MAD homolog 5
Short name:
Mothers against DPP homolog 5
Alternative name(s):
JV5-1
SMAD family member 5
Short name:
SMAD 5
Short name:
Smad5
Short name:
hSmad5
Gene namesi
Name:SMAD5
Synonyms:MADH5
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

EuPathDBiHostDB:ENSG00000113658.16
HGNCiHGNC:6771 SMAD5
MIMi603110 gene
neXtProtiNX_Q99717

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi419G → S: Loss of phosphorylation. 1 Publication1

Organism-specific databases

DisGeNETi4090
OpenTargetsiENSG00000113658
PharmGKBiPA30528

Polymorphism and mutation databases

BioMutaiSMAD5
DMDMi13959566

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources1 Publication
ChainiPRO_00000908652 – 465Mothers against decapentaplegic homolog 5Add BLAST464

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylthreonineCombined sources1 Publication1
Modified residuei463PhosphoserineCombined sources1
Modified residuei465PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated on serine by BMP (bone morphogenetic proteins) type 1 receptor kinase.
Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ99717
MaxQBiQ99717
PaxDbiQ99717
PeptideAtlasiQ99717
PRIDEiQ99717
ProteomicsDBi78432

PTM databases

iPTMnetiQ99717
PhosphoSitePlusiQ99717

Expressioni

Tissue specificityi

Ubiquitous.

Gene expression databases

BgeeiENSG00000113658 Expressed in 231 organ(s), highest expression level in female gonad
CleanExiHS_SMAD5
ExpressionAtlasiQ99717 baseline and differential
GenevisibleiQ99717 HS

Organism-specific databases

HPAiHPA058931

Interactioni

Subunit structurei

May form trimers with the co-SMAD SMAD4. Interacts with PEBP2-alpha subunit and SMURF1. Interacts with SUV39H1 and SUV39H2. Interacts (via MH2 domain) with LEMD3. Interacts with WWP1. Interacts with TMEM119 (By similarity). Interacts with ZNF8 (PubMed:12370310). Interacts with RANBP3L (PubMed:25755279).By similarity4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
DDX5P178443EBI-6391136,EBI-351962

GO - Molecular functioni

Protein-protein interaction databases

BioGridi110265, 62 interactors
DIPiDIP-57571N
IntActiQ99717, 33 interactors
MINTiQ99717
STRINGi9606.ENSP00000441954

Structurei

3D structure databases

ProteinModelPortaliQ99717
SMRiQ99717
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini13 – 137MH1PROSITE-ProRule annotationAdd BLAST125
Domaini271 – 465MH2PROSITE-ProRule annotationAdd BLAST195

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi40 – 46Poly-Lys7

Sequence similaritiesi

Belongs to the dwarfin/SMAD family.Curated

Phylogenomic databases

eggNOGiKOG3701 Eukaryota
ENOG410XQKU LUCA
GeneTreeiENSGT00760000119091
HOVERGENiHBG053353
InParanoidiQ99717
KOiK16790
OMAiDVQPVEY
OrthoDBiEOG091G082C
PhylomeDBiQ99717

Family and domain databases

Gene3Di2.60.200.10, 1 hit
3.90.520.10, 1 hit
InterProiView protein in InterPro
IPR013790 Dwarfin
IPR003619 MAD_homology1_Dwarfin-type
IPR013019 MAD_homology_MH1
IPR017855 SMAD-like_dom_sf
IPR001132 SMAD_dom_Dwarfin-type
IPR008984 SMAD_FHA_dom_sf
IPR036578 SMAD_MH1_sf
PANTHERiPTHR13703 PTHR13703, 1 hit
PfamiView protein in Pfam
PF03165 MH1, 1 hit
PF03166 MH2, 1 hit
SMARTiView protein in SMART
SM00523 DWA, 1 hit
SM00524 DWB, 1 hit
SUPFAMiSSF49879 SSF49879, 1 hit
SSF56366 SSF56366, 1 hit
PROSITEiView protein in PROSITE
PS51075 MH1, 1 hit
PS51076 MH2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 5 potential isoforms that are computationally mapped.Show allAlign All

Q99717-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTSMASLFSF TSPAVKRLLG WKQGDEEEKW AEKAVDALVK KLKKKKGAME
60 70 80 90 100
ELEKALSSPG QPSKCVTIPR SLDGRLQVSH RKGLPHVIYC RVWRWPDLQS
110 120 130 140 150
HHELKPLDIC EFPFGSKQKE VCINPYHYKR VESPVLPPVL VPRHNEFNPQ
160 170 180 190 200
HSLLVQFRNL SHNEPHMPQN ATFPDSFHQP NNTPFPLSPN SPYPPSPASS
210 220 230 240 250
TYPNSPASSG PGSPFQLPAD TPPPAYMPPD DQMGQDNSQP MDTSNNMIPQ
260 270 280 290 300
IMPSISSRDV QPVAYEEPKH WCSIVYYELN NRVGEAFHAS STSVLVDGFT
310 320 330 340 350
DPSNNKSRFC LGLLSNVNRN STIENTRRHI GKGVHLYYVG GEVYAECLSD
360 370 380 390 400
SSIFVQSRNC NFHHGFHPTT VCKIPSSCSL KIFNNQEFAQ LLAQSVNHGF
410 420 430 440 450
EAVYELTKMC TIRMSFVKGW GAEYHRQDVT STPCWIEIHL HGPLQWLDKV
460
LTQMGSPLNP ISSVS
Length:465
Mass (Da):52,258
Last modified:May 1, 1997 - v1
Checksum:i4A1FD7EC7BD06728
GO

Computationally mapped potential isoform sequencesi

There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D6RBB4D6RBB4_HUMAN
Mothers against decapentaplegic hom...
SMAD5
134Annotation score:
D6RIZ9D6RIZ9_HUMAN
Mothers against decapentaplegic hom...
SMAD5
128Annotation score:
D6R9D4D6R9D4_HUMAN
Mothers against decapentaplegic hom...
SMAD5
80Annotation score:
H0YAK7H0YAK7_HUMAN
Mothers against decapentaplegic hom...
SMAD5
174Annotation score:
H0YAM3H0YAM3_HUMAN
Mothers against decapentaplegic hom...
SMAD5
60Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti175D → H in AAC50791 (PubMed:8673135).Curated1
Sequence conflicti237N → P in AAC50791 (PubMed:8673135).Curated1
Sequence conflicti293S → R in AAC50791 (PubMed:8673135).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U59913 mRNA Translation: AAC50791.1
AF010601 mRNA Translation: AAB66353.1
AF010607
, AF010602, AF010603, AF010604, AF010605, AF010606 Genomic DNA Translation: AAB92396.1
U73825 mRNA Translation: AAB95090.1
AF009744
, AF009739, AF009740, AF009741, AF009742, AF009743 Genomic DNA Translation: AAB82655.1
AF009678 mRNA Translation: AAB72180.1
BC009682 mRNA Translation: AAH09682.1
CCDSiCCDS75308.1
RefSeqiNP_001001419.1, NM_001001419.2
NP_001001420.1, NM_001001420.2
NP_005894.3, NM_005903.6
XP_016864959.1, XM_017009470.1
UniGeneiHs.167700

Genome annotation databases

EnsembliENST00000509297; ENSP00000426696; ENSG00000113658
ENST00000545279; ENSP00000441954; ENSG00000113658
ENST00000545620; ENSP00000446474; ENSG00000113658
GeneIDi4090
KEGGihsa:4090
UCSCiuc032vlw.2 human

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U59913 mRNA Translation: AAC50791.1
AF010601 mRNA Translation: AAB66353.1
AF010607
, AF010602, AF010603, AF010604, AF010605, AF010606 Genomic DNA Translation: AAB92396.1
U73825 mRNA Translation: AAB95090.1
AF009744
, AF009739, AF009740, AF009741, AF009742, AF009743 Genomic DNA Translation: AAB82655.1
AF009678 mRNA Translation: AAB72180.1
BC009682 mRNA Translation: AAH09682.1
CCDSiCCDS75308.1
RefSeqiNP_001001419.1, NM_001001419.2
NP_001001420.1, NM_001001420.2
NP_005894.3, NM_005903.6
XP_016864959.1, XM_017009470.1
UniGeneiHs.167700

3D structure databases

ProteinModelPortaliQ99717
SMRiQ99717
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110265, 62 interactors
DIPiDIP-57571N
IntActiQ99717, 33 interactors
MINTiQ99717
STRINGi9606.ENSP00000441954

PTM databases

iPTMnetiQ99717
PhosphoSitePlusiQ99717

Polymorphism and mutation databases

BioMutaiSMAD5
DMDMi13959566

Proteomic databases

EPDiQ99717
MaxQBiQ99717
PaxDbiQ99717
PeptideAtlasiQ99717
PRIDEiQ99717
ProteomicsDBi78432

Protocols and materials databases

DNASUi4090
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000509297; ENSP00000426696; ENSG00000113658
ENST00000545279; ENSP00000441954; ENSG00000113658
ENST00000545620; ENSP00000446474; ENSG00000113658
GeneIDi4090
KEGGihsa:4090
UCSCiuc032vlw.2 human

Organism-specific databases

CTDi4090
DisGeNETi4090
EuPathDBiHostDB:ENSG00000113658.16
GeneCardsiSMAD5
HGNCiHGNC:6771 SMAD5
HPAiHPA058931
MIMi603110 gene
neXtProtiNX_Q99717
OpenTargetsiENSG00000113658
PharmGKBiPA30528
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3701 Eukaryota
ENOG410XQKU LUCA
GeneTreeiENSGT00760000119091
HOVERGENiHBG053353
InParanoidiQ99717
KOiK16790
OMAiDVQPVEY
OrthoDBiEOG091G082C
PhylomeDBiQ99717

Enzyme and pathway databases

ReactomeiR-HSA-201451 Signaling by BMP
SignaLinkiQ99717
SIGNORiQ99717

Miscellaneous databases

ChiTaRSiSMAD5 human
GeneWikiiMothers_against_decapentaplegic_homolog_5
GenomeRNAii4090
PROiPR:Q99717
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000113658 Expressed in 231 organ(s), highest expression level in female gonad
CleanExiHS_SMAD5
ExpressionAtlasiQ99717 baseline and differential
GenevisibleiQ99717 HS

Family and domain databases

Gene3Di2.60.200.10, 1 hit
3.90.520.10, 1 hit
InterProiView protein in InterPro
IPR013790 Dwarfin
IPR003619 MAD_homology1_Dwarfin-type
IPR013019 MAD_homology_MH1
IPR017855 SMAD-like_dom_sf
IPR001132 SMAD_dom_Dwarfin-type
IPR008984 SMAD_FHA_dom_sf
IPR036578 SMAD_MH1_sf
PANTHERiPTHR13703 PTHR13703, 1 hit
PfamiView protein in Pfam
PF03165 MH1, 1 hit
PF03166 MH2, 1 hit
SMARTiView protein in SMART
SM00523 DWA, 1 hit
SM00524 DWB, 1 hit
SUPFAMiSSF49879 SSF49879, 1 hit
SSF56366 SSF56366, 1 hit
PROSITEiView protein in PROSITE
PS51075 MH1, 1 hit
PS51076 MH2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSMAD5_HUMAN
AccessioniPrimary (citable) accession number: Q99717
Secondary accession number(s): O14688, Q15798, Q9UQA1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 4, 2001
Last sequence update: May 1, 1997
Last modified: November 7, 2018
This is version 193 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
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Main funding by: National Institutes of Health

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