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Protein

DNA endonuclease RBBP8

Gene

RBBP8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Endonuclease that cooperates with the MRE11-RAD50-NBN (MRN) complex in DNA-end resection, the first step of double-strand break (DSB) repair through the homologous recombination (HR) pathway. HR is restricted to S and G2 phases of the cell cycle and preferentially repairs DSBs resulting from replication fork collapse. Key determinant of DSB repair pathway choice, as it commits cells to HR by preventing classical non-homologous end-joining (NHEJ). Functions downstream of the MRN complex and ATM, promotes ATR activation and its recruitment to DSBs in the S/G2 phase facilitating the generation of ssDNA. Component of the BRCA1-RBBP8 complex that regulates CHEK1 activation and controls cell cycle G2/M checkpoints on DNA damage (PubMed:10764811, PubMed:10910365, PubMed:15485915, PubMed:16581787, PubMed:16818604, PubMed:17965729, PubMed:19202191, PubMed:19759395, PubMed:20064462, PubMed:20829486). During immunoglobulin heavy chain class-switch recombination, promotes microhomology-mediated alternative end joining (A-NHEJ) and plays an essential role in chromosomal translocations (By similarity).By similarity10 Publications

GO - Molecular functioni

  • damaged DNA binding Source: UniProtKB
  • double-strand/single-strand DNA junction binding Source: GO_Central
  • double-stranded DNA binding Source: GO_Central
  • flap-structured DNA binding Source: GO_Central
  • identical protein binding Source: IntAct
  • RNA polymerase II repressing transcription factor binding Source: BHF-UCL
  • single-stranded DNA binding Source: GO_Central
  • single-stranded DNA endodeoxyribonuclease activity Source: UniProtKB
  • transcription corepressor activity Source: BHF-UCL
  • Y-form DNA binding Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Endonuclease, Hydrolase, Nuclease
Biological processCell cycle, Cell division, DNA damage, DNA repair, Meiosis, Mitosis

Enzyme and pathway databases

ReactomeiR-HSA-5685938 HDR through Single Strand Annealing (SSA)
R-HSA-5685939 HDR through MMEJ (alt-NHEJ)
R-HSA-5685942 HDR through Homologous Recombination (HRR)
R-HSA-5693554 Resolution of D-loop Structures through Synthesis-Dependent Strand Annealing (SDSA)
R-HSA-5693568 Resolution of D-loop Structures through Holliday Junction Intermediates
R-HSA-5693579 Homologous DNA Pairing and Strand Exchange
R-HSA-5693607 Processing of DNA double-strand break ends
R-HSA-5693616 Presynaptic phase of homologous DNA pairing and strand exchange
R-HSA-6804756 Regulation of TP53 Activity through Phosphorylation
R-HSA-69473 G2/M DNA damage checkpoint
R-HSA-8953750 Transcriptional Regulation by E2F6
R-HSA-912446 Meiotic recombination
SIGNORiQ99708

Names & Taxonomyi

Protein namesi
Recommended name:
DNA endonuclease RBBP8 (EC:3.1.-.-)
Alternative name(s):
CtBP-interacting protein
Short name:
CtIP
Retinoblastoma-binding protein 8
Short name:
RBBP-8
Retinoblastoma-interacting protein and myosin-like
Short name:
RIM
Sporulation in the absence of SPO11 protein 2 homolog
Short name:
SAE2
Gene namesi
Name:RBBP8
Synonyms:CTIP
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 18

Organism-specific databases

EuPathDBiHostDB:ENSG00000101773.16
HGNCiHGNC:9891 RBBP8
MIMi604124 gene
neXtProtiNX_Q99708

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Nucleus

Pathology & Biotechi

Involvement in diseasei

Seckel syndrome 2 (SCKL2)2 Publications
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionA rare autosomal recessive disorder characterized by proportionate dwarfism of prenatal onset associated with low birth weight, growth retardation, severe microcephaly with a bird-headed like appearance, and mental retardation.
See also OMIM:606744
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_075824100R → W in SCKL2. 1 PublicationCorresponds to variant dbSNP:rs373804633EnsemblClinVar.1
Jawad syndrome (JWDS)1 Publication
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionA syndrome characterized by congenital microcephaly, moderately severe mental retardation, and symmetrical digital anomalies. Digital malformations of variable degree include hallux valgus, syndactyly of toes 4 and 5, short fifth fingers, single flexion crease of fifth fingers, polydactyly and synpolydactyly.
See also OMIM:251255
Genetic variability in RBBP8 is noted as a factor in BRCA1-associated breast cancer risk (PubMed:21799032). Associated with sensitivity to tamoxifen in certain breast cancer cell lines (PubMed:18171986).2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi31H → A: No effect on RPA focus formation on DNA damage. 1 Publication1
Mutagenesisi35V → A: No effect on RPA focus formation on DNA damage. 1 Publication1
Mutagenesisi41K → A: No effect on RPA focus formation on DNA damage. 1 Publication1
Mutagenesisi45L → A: No effect on RPA focus formation on DNA damage. 1 Publication1
Mutagenesisi62K → R in K12R; defects in ability to promoting DNA resection and homologous recombination; when associated with R-78; R-115; R-132; R-133; R-404; R-572; R-578; R-640; R-759; R-760 and R-782. In K5R; defects in ability to promoting DNA resection and homologous recombination; when associated with R-78; R-115; R-132 and R-133. 1 Publication1
Mutagenesisi78K → R in K12R; defects in ability to promoting DNA resection and homologous recombination; when associated with R-62; R-115; R-132; R-133; R-404; R-572; R-578; R-640; R-759; R-760 and R-782. In K5R; defects in ability to promoting DNA resection and homologous recombination; when associated with R-62; R-115; R-132 and R-133. 1 Publication1
Mutagenesisi115K → R in K12R; defects in ability to promoting DNA resection and homologous recombination; when associated with R-62; R-78; R-132; R-133; R-404; R-572; R-578; R-640; R-759; R-760 and R-782. In K5R; defects in ability to promoting DNA resection and homologous recombination; when associated with R-62; R-78; R-132 and R-133. 1 Publication1
Mutagenesisi132K → R in K12R; defects in ability to promoting DNA resection and homologous recombination; when associated with R-62; R-78; R-115; R-133; R-404; R-572; R-578; R-640; R-759; R-760 and R-782. In K5R; defects in ability to promoting DNA resection and homologous recombination; when associated with R-62; R-78; R-115 and R-133. 1 Publication1
Mutagenesisi133K → R in K12R; defects in ability to promoting DNA resection and homologous recombination; when associated with R-62; R-78; R-115; R-133; R-404; R-572; R-578; R-640; R-759; R-760 and R-782. In K5R; defects in ability to promoting DNA resection and homologous recombination; when associated with R-62; R-78; R-115 and R-132. 1 Publication1
Mutagenesisi179K → A: No effect on FZR1-binding. 1 Publication1
Mutagenesisi276S → A: No effect on PIN1-binding. Impaired PIN1-binding, partially decreased CUL3/KLHL15-mediated proteasomal degradation, no effect on BRCA1-, MRE11-, nor on KLHL15-binding; when associated with A-315. 2 Publications1
Mutagenesisi315T → A: Decreased PIN1-binding. Impaired PIN1-binding, partially decreased CUL3/KLHL15-mediated proteasomal degradation, no effect on BRCA1-, MRE11-, nor on KLHL15-binding; when associated with A-276. 2 Publications1
Mutagenesisi327S → A: Abolishes BRCA1 interaction and ubiquitination. No activation of CHEK1 after DNA damage. 2 Publications1
Mutagenesisi404K → R in K12R; defects in ability to promoting DNA resection and homologous recombination; when associated with R-62; R-78; R-115; R-132; R-133; R-572; R-578; R-640; R-759; R-760 and R-782. 1 Publication1
Mutagenesisi432K → R: Greatly reduced acetylation. Alleviates resection defects caused by depletion of SIRT6; when associated with R-526 and R-604. 1 Publication1
Mutagenesisi467K → A: Impaired FZR1-binding and APC/C-mediated polyubiquitination. Increased stability. No effect on MRE11-binding, nor on CUL3/KLHL15-mediated proteasomal degradation. No effect on DNA-en resection activity. 2 Publications1
Mutagenesisi513K → A: Abolishes damage recruitment capability. 1 Publication1
Mutagenesisi515K → A: Abolishes damage recruitment capability. 1 Publication1
Mutagenesisi526K → R: Greatly reduced acetylation. Alleviates resection defects caused by depletion of SIRT6; when associated with R-432 and R-604. 1 Publication1
Mutagenesisi572K → R in K12R; defects in ability to promoting DNA resection and homologous recombination; when associated with R-62; R-78; R-115; R-132; R-133; R-404; R-578; R-640; R-759; R-760 and R-782. 1 Publication1
Mutagenesisi578K → R in K12R; defects in ability to promoting DNA resection and homologous recombination; when associated with R-62; R-78; R-115; R-132; R-133; R-404; R-572; R-640; R-759; R-760 and R-782. 1 Publication1
Mutagenesisi604K → R: Greatly reduced acetylation. Alleviates resection defects caused by depletion of SIRT6; when associated with R-432 and R-526. 1 Publication1
Mutagenesisi640K → R in K12R; defects in ability to promoting DNA resection and homologous recombination; when associated with R-62; R-78; R-115; R-132; R-133; R-404; R-572; R-578; R-759; R-760 and R-782. 1 Publication1
Mutagenesisi664S → A: Abrogates dissociation of BRCA1. 1 Publication1
Mutagenesisi745S → A: Abrogates dissociation of BRCA1. 1 Publication1
Mutagenesisi759K → R in K12R; defects in ability to promoting DNA resection and homologous recombination; when associated with R-62; R-78; R-115; R-132; R-133; R-404; R-572; R-578; R-640; R-760 and R-782. 1 Publication1
Mutagenesisi760K → R in K12R; defects in ability to promoting DNA resection and homologous recombination; when associated with R-62; R-78; R-115; R-132; R-133; R-404; R-572; R-578; R-640; R-759 and R-782. 1 Publication1
Mutagenesisi782K → R in K12R; defects in ability to promoting DNA resection and homologous recombination; when associated with R-62; R-78; R-115; R-132; R-133; R-404; R-572; R-578; R-640; R-759 and R-760. 1 Publication1
Mutagenesisi839R → A: No effect on CUL3/KLHL15-mediated proteasomal degradation. 1 Publication1
Mutagenesisi840F → A: Decreased CUL3/KLHL15-mediated proteasomal degradation. 1 Publication1
Mutagenesisi842Y → A: Decreased interaction with KLHL15, decreased polyubiquitination and CUL3/KLHL15-mediated proteasomal degradation. No effect on DNA-end resection activity. 1 Publication1
Mutagenesisi842Y → F: No effect on KLHL15-binding, nor on CUL3/KLHL15-mediated proteasomal degradation. 1 Publication1
Mutagenesisi847T → A: Impairs DNA resection. 1 Publication1
Mutagenesisi847T → E: Mimics constitutive phosphorylation. 1 Publication1

Keywords - Diseasei

Disease mutation, Dwarfism, Mental retardation

Organism-specific databases

DisGeNETi5932
GeneReviewsiRBBP8
MalaCardsiRBBP8
MIMi251255 phenotype
606744 phenotype
OpenTargetsiENSG00000101773
Orphaneti313795 Jawad syndrome
808 Seckel syndrome
PharmGKBiPA34255

Polymorphism and mutation databases

BioMutaiRBBP8
DMDMi116242745

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000971791 – 897DNA endonuclease RBBP8Add BLAST897

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki62Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki115Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki193Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei233PhosphoserineCombined sources1
Modified residuei276Phosphoserine1 Publication1
Modified residuei315Phosphothreonine; by CDK2Combined sources1 Publication1
Modified residuei326Phosphoserine1 Publication1
Modified residuei327PhosphoserineCombined sources1 Publication1
Modified residuei349Phosphoserine1 Publication1
Cross-linki360Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki378Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei379PhosphoserineCombined sources1
Cross-linki396Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki404Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki410Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei432N6-acetyllysine1 Publication1
Cross-linki438Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki449Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei526N6-acetyllysine; alternate1 Publication1
Cross-linki526Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateCombined sources
Cross-linki530Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki572Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki578Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei604N6-acetyllysine; alternate1 Publication1
Cross-linki604Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateCombined sources
Cross-linki613Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki638Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki640Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei664Phosphoserine; by ATM1 Publication1
Cross-linki676Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei679Phosphoserine1 Publication1
Cross-linki719Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei723PhosphoserineCombined sources1
Modified residuei745Phosphoserine; by ATM1 Publication1
Cross-linki782Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei847Phosphothreonine; by CDK11 Publication1
Cross-linki869Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources

Post-translational modificationi

Acetylated. Deacetylation by SIRT6 upon DNA damage promotes DNA end resection.1 Publication
Hyperphosphorylation upon ionizing radiation results in dissociation from BRCA1. Phosphorylation at Thr-847 by CDK1 is essential for the recruitment to DNA and the DNA repair function. Phosphorylated on Ser-327 as cells enter G2 phase. This phosphorylation is required for binding BRCA1 and for the G2/M DNA damage transition checkpoint control. Phosphorylation at Thr-315, probably catalyzed by CDK2, is required for PIN1-binding, while phosphorylation at Ser-276 serves as a PIN1 isomerization site. Phosphorylation at Thr-315 is cell-cycle dependent. It steadily increases during S phase, peaks at late S/G2 phase, and drops at G1 (PubMed:23623683).5 Publications
Ubiquitinated (PubMed:14654780, PubMed:16818604, PubMed:27561354). Ubiquitination at multiple sites by BRCA1 (via its N-terminal RING domain) does not lead to its proteosomal degradation but instead the ubiquitinated RBBP8 binds to chromatin following DNA damage and may play a role in G2/M checkpoint control (PubMed:16818604). Ubiquitinated by RNF138 at its N-terminus (PubMed:26502057). Ubiquitinated through 'Lys-48' by the E3 CUL3-KLHL15 complex; this modification leads to proteasomal degradation (PubMed:27561354). Ubiquitinated by the E3 FZR1/APC/C complex; this modification leads to proteasomal degradation (PubMed:25349192).5 Publications

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ99708
MaxQBiQ99708
PaxDbiQ99708
PeptideAtlasiQ99708
PRIDEiQ99708
ProteomicsDBi78424
78425 [Q99708-2]

PTM databases

iPTMnetiQ99708
PhosphoSitePlusiQ99708

Expressioni

Tissue specificityi

Expressed in ER-positive breast cancer lines, but tends to be down-regulated ER-negative cells (at protein level).1 Publication

Inductioni

Expression is cell-cycle regulated. Levels increase as dividing cells traverse the G1/S boundary (PubMed:18171986). The protein is degraded by the proteasome pathway during mitotic exit. Also degraded in response to DNA damage in G2 cells; this degradation is mediated by the E3 FZR1/APC/C complex (PubMed:25349192).2 Publications

Gene expression databases

BgeeiENSG00000101773 Expressed in 212 organ(s), highest expression level in testis
CleanExiHS_RBBP8
ExpressionAtlasiQ99708 baseline and differential
GenevisibleiQ99708 HS

Organism-specific databases

HPAiHPA039890
HPA052946

Interactioni

Subunit structurei

Homodimer; dimerizes via the coiled coil domain (PubMed:15084581). Interacts (via the PXDLS motif) with CTBP1; the interaction is disrupted via binding of the adenovirus E1A to CTBP1 (PubMed:9535825). Component of the BRCA1-RBBP8 complex. Interacts (the Ser-327 phosphorylated form) with BRCA1 (via the C-terminal BRCA1 domains): the interaction occurs in the G2 phase, ubiquitinates RBBP8 and involves RBBP8 in BRCA1-dependent G2/M checkpoint control on DNA damage (PubMed:10764811, PubMed:15485915, PubMed:16818604, PubMed:17965729, PubMed:23623683). Interacts with RB1 (PubMed:9721205). Interacts with the MRN complex. Interacts directly with MRE11; the interaction is required for efficient homologous recombination (HR) and regulation of the MRN complex (PubMed:19759395, PubMed:23623683). Interacts directly with RAD50 (PubMed:19759395). Interacts directly with NBN (PubMed:19759395). Interacts with SIRT6; the interaction deacetylates RBBP8 upon DNA damage (PubMed:20829486). Interacts with LM04 (via the LIM zinc-binding 1 domain) (PubMed:11751867). Interacts with SIAH1 (PubMed:14654780). Interacts with RNF138 (PubMed:26502057). Interacts with EXD2 (PubMed:26807646). Interacts with CUL3 and KLHL15; this interaction leads to RBBP8 proteasomal degradation (PubMed:27561354). Directly interacts with PIN1; this interaction depends upon RBBP8 phosphorylation, predominantly at Thr-315 (PubMed:23623683). Interacts with FZR1; this interaction leads to APC/C-mediated RBBP8 proteasomal degradation (PubMed:25349192). Interacts with AUNIP; leading to recruit RBBP8 to sites of DNA damage (PubMed:29042561, PubMed:10764811, PubMed:11751867, PubMed:14654780, PubMed:15084581, PubMed:15485915, PubMed:16818604, PubMed:17965729, PubMed:19759395, PubMed:20829486, PubMed:23623683, PubMed:25349192, PubMed:26502057, PubMed:26807646, PubMed:27561354, PubMed:9535825, PubMed:9721205). Interacts with SAMHD1 (PubMed:28834754).18 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi111867, 73 interactors
CORUMiQ99708
DIPiDIP-24244N
ELMiQ99708
IntActiQ99708, 42 interactors
MINTiQ99708
STRINGi9606.ENSP00000323050

Structurei

Secondary structure

1897
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ99708
SMRiQ99708
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ99708

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni22 – 45Essential for binding to the MRN complex and for RPA focus formation on DNA damageAdd BLAST24
Regioni509 – 557Damage-recruitment motifAdd BLAST49

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili28 – 157Sequence analysisAdd BLAST130

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi490 – 494PXDLS motif5
Motifi840 – 842KLHL15-binding1 Publication3

Domaini

The PXDLS motif binds to a cleft in CtBP proteins.
The damage-recruitment motif is required for DNA binding and translocation to sites of DNA damage.

Sequence similaritiesi

Belongs to the COM1/SAE2/CtIP family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IJ39 Eukaryota
ENOG410ZSBE LUCA
GeneTreeiENSGT00530000063835
HOGENOMiHOG000293331
HOVERGENiHBG057046
InParanoidiQ99708
KOiK20773
PhylomeDBiQ99708
TreeFamiTF106469

Family and domain databases

InterProiView protein in InterPro
IPR019518 CtIP_N
IPR013882 Ctp1_C
IPR033594 RBBP8
IPR033316 RBBP8-like
PANTHERiPTHR15107 PTHR15107, 1 hit
PTHR15107:SF4 PTHR15107:SF4, 1 hit
PfamiView protein in Pfam
PF10482 CtIP_N, 1 hit
PF08573 SAE2, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 11 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q99708-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MNISGSSCGS PNSADTSSDF KDLWTKLKEC HDREVQGLQV KVTKLKQERI
60 70 80 90 100
LDAQRLEEFF TKNQQLREQQ KVLHETIKVL EDRLRAGLCD RCAVTEEHMR
110 120 130 140 150
KKQQEFENIR QQNLKLITEL MNERNTLQEE NKKLSEQLQQ KIENDQQHQA
160 170 180 190 200
AELECEEDVI PDSPITAFSF SGVNRLRRKE NPHVRYIEQT HTKLEHSVCA
210 220 230 240 250
NEMRKVSKSS THPQHNPNEN EILVADTYDQ SQSPMAKAHG TSSYTPDKSS
260 270 280 290 300
FNLATVVAET LGLGVQEESE TQGPMSPLGD ELYHCLEGNH KKQPFEESTR
310 320 330 340 350
NTEDSLRFSD STSKTPPQEE LPTRVSSPVF GATSSIKSGL DLNTSLSPSL
360 370 380 390 400
LQPGKKKHLK TLPFSNTCIS RLEKTRSKSE DSALFTHHSL GSEVNKIIIQ
410 420 430 440 450
SSNKQILINK NISESLGEQN RTEYGKDSNT DKHLEPLKSL GGRTSKRKKT
460 470 480 490 500
EEESEHEVSC PQASFDKENA FPFPMDNQFS MNGDCVMDKP LDLSDRFSAI
510 520 530 540 550
QRQEKSQGSE TSKNKFRQVT LYEALKTIPK GFSSSRKASD GNCTLPKDSP
560 570 580 590 600
GEPCSQECII LQPLNKCSPD NKPSLQIKEE NAVFKIPLRP RESLETENVL
610 620 630 640 650
DDIKSAGSHE PIKIQTRSDH GGCELASVLQ LNPCRTGKIK SLQNNQDVSF
660 670 680 690 700
ENIQWSIDPG ADLSQYKMDV TVIDTKDGSQ SKLGGETVDM DCTLVSETVL
710 720 730 740 750
LKMKKQEQKG EKSSNEERKM NDSLEDMFDR TTHEEYESCL ADSFSQAADE
760 770 780 790 800
EEELSTATKK LHTHGDKQDK VKQKAFVEPY FKGDERETSL QNFPHIEVVR
810 820 830 840 850
KKEERRKLLG HTCKECEIYY ADMPAEEREK KLASCSRHRF RYIPPNTPEN
860 870 880 890
FWEVGFPSTQ TCMERGYIKE DLDPCPRPKR RQPYNAIFSP KGKEQKT
Length:897
Mass (Da):101,942
Last modified:October 17, 2006 - v2
Checksum:iE028DE56DE55C0F2
GO
Isoform 2 (identifier: Q99708-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     714-714: S → SMLFYI

Show »
Length:902
Mass (Da):102,610
Checksum:i43DEA11A0349322B
GO
Isoform 3 (identifier: Q99708-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     786-867: RETSLQNFPH...STQTCMERGY → SIMQICQQKK...QKARSRRHRR
     868-897: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:867
Mass (Da):98,434
Checksum:iA8CFDB72587619E3
GO

Computationally mapped potential isoform sequencesi

There are 11 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
I6L8A6I6L8A6_HUMAN
DNA endonuclease RBBP8
RBBP8
902Annotation score:
F6Q6H0F6Q6H0_HUMAN
DNA endonuclease RBBP8
RBBP8
580Annotation score:
J3QLW6J3QLW6_HUMAN
DNA endonuclease RBBP8
RBBP8
205Annotation score:
J3QL00J3QL00_HUMAN
DNA endonuclease RBBP8
RBBP8
160Annotation score:
J3QL93J3QL93_HUMAN
DNA endonuclease RBBP8
RBBP8
84Annotation score:
J3KSA4J3KSA4_HUMAN
DNA endonuclease RBBP8
RBBP8
64Annotation score:
J3QLH2J3QLH2_HUMAN
DNA endonuclease RBBP8
RBBP8
75Annotation score:
J3QSH7J3QSH7_HUMAN
DNA endonuclease RBBP8
RBBP8
324Annotation score:
J3QRM0J3QRM0_HUMAN
DNA endonuclease RBBP8
RBBP8
29Annotation score:
V9GZ40V9GZ40_HUMAN
DNA endonuclease RBBP8
RBBP8
45Annotation score:
There is more potential isoformShow all

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti4S → L in AAC14371 (PubMed:9721205).Curated1
Sequence conflicti74H → Q in BX648221 (PubMed:17974005).Curated1
Sequence conflicti92C → Y in BAF85170 (PubMed:14702039).Curated1
Sequence conflicti123E → G in BAF85170 (PubMed:14702039).Curated1
Sequence conflicti341D → G in BX648221 (PubMed:17974005).Curated1
Sequence conflicti515K → R in BX648221 (PubMed:17974005).Curated1
Sequence conflicti521L → P in BAF85170 (PubMed:14702039).Curated1
Sequence conflicti642L → P in BX648221 (PubMed:17974005).Curated1
Isoform 3 (identifier: Q99708-3)
Sequence conflicti862S → G in BX648221 (PubMed:17974005).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_075824100R → W in SCKL2. 1 PublicationCorresponds to variant dbSNP:rs373804633EnsemblClinVar.1
Natural variantiVAR_051308357K → N. Corresponds to variant dbSNP:rs34678569EnsemblClinVar.1
Natural variantiVAR_028308387H → Y. Corresponds to variant dbSNP:rs1804732Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_043220714S → SMLFYI in isoform 2. 1 Publication1
Alternative sequenceiVSP_045247786 – 867RETSL…MERGY → SIMQICQQKKEKRNWLPAQD TDSATFHPTHQRIFGKLVFL PLRLVWKEVILRKILILVLV QKDVSLTTQYFLQKARSRRH RR in isoform 3. 1 PublicationAdd BLAST82
Alternative sequenceiVSP_045248868 – 897Missing in isoform 3. 1 PublicationAdd BLAST30

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF043431 mRNA Translation: AAC34368.1
U72066 mRNA Translation: AAC14371.1
AK292481 mRNA Translation: BAF85170.1
BX648221 mRNA No translation available.
AC091147 Genomic DNA No translation available.
AC106033 Genomic DNA No translation available.
CH471088 Genomic DNA Translation: EAX01144.1
BC030590 mRNA Translation: AAH30590.1
CCDSiCCDS11874.1 [Q99708-3]
CCDS11875.1 [Q99708-1]
RefSeqiNP_002885.1, NM_002894.2 [Q99708-1]
NP_976036.1, NM_203291.1 [Q99708-1]
NP_976037.1, NM_203292.1 [Q99708-3]
XP_006722582.1, XM_006722519.2 [Q99708-1]
XP_006722583.1, XM_006722520.2 [Q99708-1]
XP_006722584.1, XM_006722521.2 [Q99708-1]
XP_011524434.1, XM_011526132.2 [Q99708-1]
UniGeneiHs.546282

Genome annotation databases

EnsembliENST00000327155; ENSP00000323050; ENSG00000101773 [Q99708-1]
ENST00000399722; ENSP00000382628; ENSG00000101773 [Q99708-1]
ENST00000399725; ENSP00000382630; ENSG00000101773 [Q99708-3]
GeneIDi5932
KEGGihsa:5932
UCSCiuc002ktw.4 human [Q99708-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF043431 mRNA Translation: AAC34368.1
U72066 mRNA Translation: AAC14371.1
AK292481 mRNA Translation: BAF85170.1
BX648221 mRNA No translation available.
AC091147 Genomic DNA No translation available.
AC106033 Genomic DNA No translation available.
CH471088 Genomic DNA Translation: EAX01144.1
BC030590 mRNA Translation: AAH30590.1
CCDSiCCDS11874.1 [Q99708-3]
CCDS11875.1 [Q99708-1]
RefSeqiNP_002885.1, NM_002894.2 [Q99708-1]
NP_976036.1, NM_203291.1 [Q99708-1]
NP_976037.1, NM_203292.1 [Q99708-3]
XP_006722582.1, XM_006722519.2 [Q99708-1]
XP_006722583.1, XM_006722520.2 [Q99708-1]
XP_006722584.1, XM_006722521.2 [Q99708-1]
XP_011524434.1, XM_011526132.2 [Q99708-1]
UniGeneiHs.546282

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2L4ZNMR-A641-685[»]
4D2HX-ray1.90A/B/C/D/E/F/G/H18-52[»]
ProteinModelPortaliQ99708
SMRiQ99708
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111867, 73 interactors
CORUMiQ99708
DIPiDIP-24244N
ELMiQ99708
IntActiQ99708, 42 interactors
MINTiQ99708
STRINGi9606.ENSP00000323050

PTM databases

iPTMnetiQ99708
PhosphoSitePlusiQ99708

Polymorphism and mutation databases

BioMutaiRBBP8
DMDMi116242745

Proteomic databases

EPDiQ99708
MaxQBiQ99708
PaxDbiQ99708
PeptideAtlasiQ99708
PRIDEiQ99708
ProteomicsDBi78424
78425 [Q99708-2]

Protocols and materials databases

DNASUi5932
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000327155; ENSP00000323050; ENSG00000101773 [Q99708-1]
ENST00000399722; ENSP00000382628; ENSG00000101773 [Q99708-1]
ENST00000399725; ENSP00000382630; ENSG00000101773 [Q99708-3]
GeneIDi5932
KEGGihsa:5932
UCSCiuc002ktw.4 human [Q99708-1]

Organism-specific databases

CTDi5932
DisGeNETi5932
EuPathDBiHostDB:ENSG00000101773.16
GeneCardsiRBBP8
GeneReviewsiRBBP8
HGNCiHGNC:9891 RBBP8
HPAiHPA039890
HPA052946
MalaCardsiRBBP8
MIMi251255 phenotype
604124 gene
606744 phenotype
neXtProtiNX_Q99708
OpenTargetsiENSG00000101773
Orphaneti313795 Jawad syndrome
808 Seckel syndrome
PharmGKBiPA34255
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IJ39 Eukaryota
ENOG410ZSBE LUCA
GeneTreeiENSGT00530000063835
HOGENOMiHOG000293331
HOVERGENiHBG057046
InParanoidiQ99708
KOiK20773
PhylomeDBiQ99708
TreeFamiTF106469

Enzyme and pathway databases

ReactomeiR-HSA-5685938 HDR through Single Strand Annealing (SSA)
R-HSA-5685939 HDR through MMEJ (alt-NHEJ)
R-HSA-5685942 HDR through Homologous Recombination (HRR)
R-HSA-5693554 Resolution of D-loop Structures through Synthesis-Dependent Strand Annealing (SDSA)
R-HSA-5693568 Resolution of D-loop Structures through Holliday Junction Intermediates
R-HSA-5693579 Homologous DNA Pairing and Strand Exchange
R-HSA-5693607 Processing of DNA double-strand break ends
R-HSA-5693616 Presynaptic phase of homologous DNA pairing and strand exchange
R-HSA-6804756 Regulation of TP53 Activity through Phosphorylation
R-HSA-69473 G2/M DNA damage checkpoint
R-HSA-8953750 Transcriptional Regulation by E2F6
R-HSA-912446 Meiotic recombination
SIGNORiQ99708

Miscellaneous databases

ChiTaRSiRBBP8 human
EvolutionaryTraceiQ99708
GeneWikiiRBBP8
GenomeRNAii5932
PROiPR:Q99708
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000101773 Expressed in 212 organ(s), highest expression level in testis
CleanExiHS_RBBP8
ExpressionAtlasiQ99708 baseline and differential
GenevisibleiQ99708 HS

Family and domain databases

InterProiView protein in InterPro
IPR019518 CtIP_N
IPR013882 Ctp1_C
IPR033594 RBBP8
IPR033316 RBBP8-like
PANTHERiPTHR15107 PTHR15107, 1 hit
PTHR15107:SF4 PTHR15107:SF4, 1 hit
PfamiView protein in Pfam
PF10482 CtIP_N, 1 hit
PF08573 SAE2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCTIP_HUMAN
AccessioniPrimary (citable) accession number: Q99708
Secondary accession number(s): A6NKN2
, A8K8W6, E7ETY1, O75371, Q8NHQ3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: October 17, 2006
Last modified: November 7, 2018
This is version 176 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human chromosome 18
    Human chromosome 18: entries, gene names and cross-references to MIM
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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