UniProtKB - Q99661 (KIF2C_HUMAN)
Kinesin-like protein KIF2C
KIF2C
Functioni
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 264 | ATPBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 348 – 355 | ATP | 8 |
GO - Molecular functioni
- ATPase activity Source: GO_Central
- ATP binding Source: UniProtKB-KW
- centromeric DNA binding Source: ProtInc
- microtubule binding Source: GO_Central
- microtubule motor activity Source: GO_Central
- microtubule plus-end binding Source: UniProtKB
GO - Biological processi
- antigen processing and presentation of exogenous peptide antigen via MHC class II Source: Reactome
- attachment of mitotic spindle microtubules to kinetochore Source: UniProtKB
- cell division Source: UniProtKB-KW
- establishment or maintenance of microtubule cytoskeleton polarity Source: HGNC-UCL
- metaphase plate congression Source: UniProtKB
- microtubule-based movement Source: GO_Central
- microtubule depolymerization Source: UniProtKB
- mitotic metaphase plate congression Source: UniProtKB
- regulation of chromosome segregation Source: UniProtKB
- retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum Source: Reactome
Keywordsi
Biological process | Cell cycle, Cell division, Chromosome partition, Mitosis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
PathwayCommonsi | Q99661 |
Reactomei | R-HSA-141444, Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal R-HSA-2132295, MHC class II antigen presentation R-HSA-2467813, Separation of Sister Chromatids R-HSA-2500257, Resolution of Sister Chromatid Cohesion R-HSA-5663220, RHO GTPases Activate Formins R-HSA-6811434, COPI-dependent Golgi-to-ER retrograde traffic R-HSA-68877, Mitotic Prometaphase R-HSA-9648025, EML4 and NUDC in mitotic spindle formation R-HSA-983189, Kinesins |
SIGNORi | Q99661 |
Names & Taxonomyi
Protein namesi | Recommended name: Kinesin-like protein KIF2CAlternative name(s): Kinesin-like protein 6 Mitotic centromere-associated kinesin Short name: MCAK |
Gene namesi | Name:KIF2C Synonyms:KNSL6 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:6393, KIF2C |
MIMi | 604538, gene |
neXtProti | NX_Q99661 |
VEuPathDBi | HostDB:ENSG00000142945.12 |
Subcellular locationi
Cytoskeleton
- cytoskeleton 3 Publications
Nucleus
- Nucleus By similarity
Other locations
- centromere 2 Publications
- kinetochore 3 Publications
Note: Associates with the microtubule network at the growing distal tip (the plus-end) of microtubules, probably through interaction with MTUS2/TIP150 and MAPRE1 (By similarity). Association with microtubule plus ends is also mediated by interaction with KIF18B. Centromeric localization requires the presence of BUB1 and SGO2.By similarity2 Publications
Cytoskeleton
- centrosome Source: GO_Central
- kinesin complex Source: GO_Central
- microtubule Source: GO_Central
- microtubule cytoskeleton Source: LIFEdb
- microtubule plus-end Source: UniProtKB
- spindle Source: GO_Central
Cytosol
- cytosol Source: Reactome
Nucleus
- nucleus Source: UniProtKB-SubCell
Other locations
- chromosome, centromeric region Source: UniProtKB
- condensed chromosome kinetochore Source: UniProtKB-SubCell
- kinetochore Source: UniProtKB
- membrane Source: UniProtKB
Keywords - Cellular componenti
Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, Microtubule, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 95 | S → E: Alters interaction with MAPRE1 and association with microtubule growing ends; when associated with E-109 and E-111. 1 Publication | 1 | |
Mutagenesisi | 100 – 101 | IP → NN: Loss of interaction with MAPRE1 and association with microtubule growing ends. 1 Publication | 2 | |
Mutagenesisi | 109 | S → E: Alters interaction with MAPRE1 and association with microtubule growing ends; when associated with E-95 and E-111. 1 Publication | 1 | |
Mutagenesisi | 111 | S → E: Alters interaction with MAPRE1 and association with microtubule growing ends; when associated with E-95 and E-109. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 11004 |
OpenTargetsi | ENSG00000142945 |
PharmGKBi | PA30182 |
Miscellaneous databases
Pharosi | Q99661, Tbio |
Chemistry databases
ChEMBLi | CHEMBL5967 |
DrugBanki | DB04395, Phosphoaminophosphonic Acid-Adenylate Ester |
Genetic variation databases
BioMutai | KIF2C |
DMDMi | 20141607 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000125418 | 2 – 725 | Kinesin-like protein KIF2CAdd BLAST | 724 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources | 1 | |
Modified residuei | 6 | PhosphoserineCombined sources | 1 | |
Modified residuei | 22 | PhosphoserineCombined sources | 1 | |
Modified residuei | 95 | Phosphoserine; by AURKB1 Publication | 1 | |
Modified residuei | 106 | PhosphoserineCombined sources | 1 | |
Modified residuei | 109 | PhosphoserineBy similarity | 1 | |
Modified residuei | 111 | PhosphoserineBy similarity | 1 | |
Modified residuei | 115 | PhosphoserineCombined sources | 1 | |
Modified residuei | 166 | PhosphoserineCombined sources | 1 | |
Modified residuei | 175 | PhosphoserineCombined sources | 1 | |
Modified residuei | 187 | PhosphoserineCombined sources | 1 | |
Modified residuei | 192 | PhosphoserineBy similarity | 1 | |
Modified residuei | 519 | PhosphoserineCombined sources | 1 | |
Modified residuei | 621 | PhosphoserineCombined sources | 1 | |
Modified residuei | 633 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q99661 |
jPOSTi | Q99661 |
MassIVEi | Q99661 |
MaxQBi | Q99661 |
PaxDbi | Q99661 |
PeptideAtlasi | Q99661 |
PRIDEi | Q99661 |
ProteomicsDBi | 78382 [Q99661-1] 78383 [Q99661-2] |
PTM databases
iPTMneti | Q99661 |
MetOSitei | Q99661 |
PhosphoSitePlusi | Q99661 |
SwissPalmi | Q99661 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSG00000142945, Expressed in testis and 159 other tissues |
ExpressionAtlasi | Q99661, baseline and differential |
Genevisiblei | Q99661, HS |
Organism-specific databases
HPAi | ENSG00000142945, Tissue enhanced (lymphoid tissue, testis) |
Interactioni
Subunit structurei
Interacts with CENPH.
Interacts with MTUS2/TIP150; the interaction is direct.
Interacts with MAPRE1; the interaction is direct, regulated by phosphorylation and is probably required for targeting to growing microtubule plus ends.
Interacts with KIF18B at microtubule tips; this interaction increases the affinity of both partners for microtubule plus ends and is required for robust microtubule depolymerization. Phosphorylation by AURKA or AURKB strongly reduces KIF18B-binding.
5 PublicationsBinary interactionsi
Hide detailsGO - Molecular functioni
- microtubule binding Source: GO_Central
- microtubule plus-end binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 116195, 101 interactors |
ELMi | Q99661 |
IntActi | Q99661, 61 interactors |
MINTi | Q99661 |
STRINGi | 9606.ENSP00000361298 |
Chemistry databases
BindingDBi | Q99661 |
Miscellaneous databases
RNActi | Q99661, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q99661 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q99661 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 258 – 588 | Kinesin motorPROSITE-ProRule annotationAdd BLAST | 331 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 2 – 254 | GlobularSequence analysisAdd BLAST | 253 | |
Regioni | 207 – 238 | Negative regulator of microtubule-bindingBy similarityAdd BLAST | 32 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 618 – 658 | Sequence analysisAdd BLAST | 41 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 98 – 101 | Microtubule tip localization signal | 4 | |
Motifi | 415 – 418 | Nuclear localization signalSequence analysis | 4 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG0246, Eukaryota |
GeneTreei | ENSGT00940000154046 |
HOGENOMi | CLU_001485_19_1_1 |
InParanoidi | Q99661 |
OMAi | RTTLECH |
PhylomeDBi | Q99661 |
TreeFami | TF105222 |
Family and domain databases
Gene3Di | 3.40.850.10, 1 hit |
InterProi | View protein in InterPro IPR027640, Kinesin-like_fam IPR019821, Kinesin_motor_CS IPR001752, Kinesin_motor_dom IPR036961, Kinesin_motor_dom_sf IPR027417, P-loop_NTPase |
PANTHERi | PTHR24115, PTHR24115, 1 hit |
Pfami | View protein in Pfam PF00225, Kinesin, 1 hit |
PRINTSi | PR00380, KINESINHEAVY |
SMARTi | View protein in SMART SM00129, KISc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00411, KINESIN_MOTOR_1, 1 hit PS50067, KINESIN_MOTOR_2, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAMDSSLQAR LFPGLAIKIQ RSNGLIHSAN VRTVNLEKSC VSVEWAEGGA
60 70 80 90 100
TKGKEIDFDD VAAINPELLQ LLPLHPKDNL PLQENVTIQK QKRRSVNSKI
110 120 130 140 150
PAPKESLRSR STRMSTVSEL RITAQENDME VELPAAANSR KQFSVPPAPT
160 170 180 190 200
RPSCPAVAEI PLRMVSEEME EQVHSIRGSS SANPVNSVRR KSCLVKEVEK
210 220 230 240 250
MKNKREEKKA QNSEMRMKRA QEYDSSFPNW EFARMIKEFR ATLECHPLTM
260 270 280 290 300
TDPIEEHRIC VCVRKRPLNK QELAKKEIDV ISIPSKCLLL VHEPKLKVDL
310 320 330 340 350
TKYLENQAFC FDFAFDETAS NEVVYRFTAR PLVQTIFEGG KATCFAYGQT
360 370 380 390 400
GSGKTHTMGG DLSGKAQNAS KGIYAMASRD VFLLKNQPCY RKLGLEVYVT
410 420 430 440 450
FFEIYNGKLF DLLNKKAKLR VLEDGKQQVQ VVGLQEHLVN SADDVIKMID
460 470 480 490 500
MGSACRTSGQ TFANSNSSRS HACFQIILRA KGRMHGKFSL VDLAGNERGA
510 520 530 540 550
DTSSADRQTR MEGAEINKSL LALKECIRAL GQNKAHTPFR ESKLTQVLRD
560 570 580 590 600
SFIGENSRTC MIATISPGIS SCEYTLNTLR YADRVKELSP HSGPSGEQLI
610 620 630 640 650
QMETEEMEAC SNGALIPGNL SKEEEELSSQ MSSFNEAMTQ IRELEEKAME
660 670 680 690 700
ELKEIIQQGP DWLELSEMTE QPDYDLETFV NKAESALAQQ AKHFSALRDV
710 720
IKALRLAMQL EEQASRQISS KKRPQ
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ5JR91 | Q5JR91_HUMAN | Kinesin-like protein KIF2C | KIF2C | 336 | Annotation score: | ||
Q5JR89 | Q5JR89_HUMAN | Kinesin-like protein KIF2C | KIF2C | 287 | Annotation score: | ||
H0Y5Z9 | H0Y5Z9_HUMAN | Kinesin-like protein KIF2C | KIF2C | 107 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 698 | R → P in AAH08764 (PubMed:15489334).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_049683 | 449 | I → L3 PublicationsCorresponds to variant dbSNP:rs4342887Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_002866 | 1 – 55 | MAMDS…TKGKE → M in isoform 2. 1 PublicationAdd BLAST | 55 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U63743 mRNA Translation: AAC27660.1 AY026505 mRNA Translation: AAK20168.1 BT006759 mRNA Translation: AAP35405.1 CR450302 mRNA Translation: CAG29298.1 AB264115 mRNA Translation: BAG50306.1 AL592166 Genomic DNA No translation available. CH471059 Genomic DNA Translation: EAX07025.1 BC008764 mRNA Translation: AAH08764.1 BC014924 mRNA Translation: AAH14924.1 |
CCDSi | CCDS512.1 [Q99661-1] CCDS72774.1 [Q99661-2] |
RefSeqi | NP_001284584.1, NM_001297655.1 NP_001284585.1, NM_001297656.1 [Q99661-2] NP_001284586.1, NM_001297657.1 NP_006836.2, NM_006845.3 [Q99661-1] |
Genome annotation databases
Ensembli | ENST00000372217; ENSP00000361291; ENSG00000142945 [Q99661-2] ENST00000372224; ENSP00000361298; ENSG00000142945 [Q99661-1] |
GeneIDi | 11004 |
KEGGi | hsa:11004 |
UCSCi | uc001cmg.5, human [Q99661-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U63743 mRNA Translation: AAC27660.1 AY026505 mRNA Translation: AAK20168.1 BT006759 mRNA Translation: AAP35405.1 CR450302 mRNA Translation: CAG29298.1 AB264115 mRNA Translation: BAG50306.1 AL592166 Genomic DNA No translation available. CH471059 Genomic DNA Translation: EAX07025.1 BC008764 mRNA Translation: AAH08764.1 BC014924 mRNA Translation: AAH14924.1 |
CCDSi | CCDS512.1 [Q99661-1] CCDS72774.1 [Q99661-2] |
RefSeqi | NP_001284584.1, NM_001297655.1 NP_001284585.1, NM_001297656.1 [Q99661-2] NP_001284586.1, NM_001297657.1 NP_006836.2, NM_006845.3 [Q99661-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2HEH | X-ray | 2.15 | A | 225-593 | [»] | |
4UBF | X-ray | 3.00 | A/B/C/D | 225-593 | [»] | |
P | 709-720 | [»] | ||||
4Y05 | X-ray | 2.59 | A | 216-599 | [»] | |
5MIO | X-ray | 3.19 | C | 216-598 | [»] | |
SMRi | Q99661 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 116195, 101 interactors |
ELMi | Q99661 |
IntActi | Q99661, 61 interactors |
MINTi | Q99661 |
STRINGi | 9606.ENSP00000361298 |
Chemistry databases
BindingDBi | Q99661 |
ChEMBLi | CHEMBL5967 |
DrugBanki | DB04395, Phosphoaminophosphonic Acid-Adenylate Ester |
PTM databases
iPTMneti | Q99661 |
MetOSitei | Q99661 |
PhosphoSitePlusi | Q99661 |
SwissPalmi | Q99661 |
Genetic variation databases
BioMutai | KIF2C |
DMDMi | 20141607 |
Proteomic databases
EPDi | Q99661 |
jPOSTi | Q99661 |
MassIVEi | Q99661 |
MaxQBi | Q99661 |
PaxDbi | Q99661 |
PeptideAtlasi | Q99661 |
PRIDEi | Q99661 |
ProteomicsDBi | 78382 [Q99661-1] 78383 [Q99661-2] |
Protocols and materials databases
Antibodypediai | 1212, 365 antibodies |
CPTCi | Q99661, 3 antibodies |
DNASUi | 11004 |
Genome annotation databases
Ensembli | ENST00000372217; ENSP00000361291; ENSG00000142945 [Q99661-2] ENST00000372224; ENSP00000361298; ENSG00000142945 [Q99661-1] |
GeneIDi | 11004 |
KEGGi | hsa:11004 |
UCSCi | uc001cmg.5, human [Q99661-1] |
Organism-specific databases
CTDi | 11004 |
DisGeNETi | 11004 |
GeneCardsi | KIF2C |
HGNCi | HGNC:6393, KIF2C |
HPAi | ENSG00000142945, Tissue enhanced (lymphoid tissue, testis) |
MIMi | 604538, gene |
neXtProti | NX_Q99661 |
OpenTargetsi | ENSG00000142945 |
PharmGKBi | PA30182 |
VEuPathDBi | HostDB:ENSG00000142945.12 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0246, Eukaryota |
GeneTreei | ENSGT00940000154046 |
HOGENOMi | CLU_001485_19_1_1 |
InParanoidi | Q99661 |
OMAi | RTTLECH |
PhylomeDBi | Q99661 |
TreeFami | TF105222 |
Enzyme and pathway databases
PathwayCommonsi | Q99661 |
Reactomei | R-HSA-141444, Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal R-HSA-2132295, MHC class II antigen presentation R-HSA-2467813, Separation of Sister Chromatids R-HSA-2500257, Resolution of Sister Chromatid Cohesion R-HSA-5663220, RHO GTPases Activate Formins R-HSA-6811434, COPI-dependent Golgi-to-ER retrograde traffic R-HSA-68877, Mitotic Prometaphase R-HSA-9648025, EML4 and NUDC in mitotic spindle formation R-HSA-983189, Kinesins |
SIGNORi | Q99661 |
Miscellaneous databases
BioGRID-ORCSi | 11004, 164 hits in 887 CRISPR screens |
ChiTaRSi | KIF2C, human |
EvolutionaryTracei | Q99661 |
GeneWikii | KIF2C |
GenomeRNAii | 11004 |
Pharosi | Q99661, Tbio |
PROi | PR:Q99661 |
RNActi | Q99661, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000142945, Expressed in testis and 159 other tissues |
ExpressionAtlasi | Q99661, baseline and differential |
Genevisiblei | Q99661, HS |
Family and domain databases
Gene3Di | 3.40.850.10, 1 hit |
InterProi | View protein in InterPro IPR027640, Kinesin-like_fam IPR019821, Kinesin_motor_CS IPR001752, Kinesin_motor_dom IPR036961, Kinesin_motor_dom_sf IPR027417, P-loop_NTPase |
PANTHERi | PTHR24115, PTHR24115, 1 hit |
Pfami | View protein in Pfam PF00225, Kinesin, 1 hit |
PRINTSi | PR00380, KINESINHEAVY |
SMARTi | View protein in SMART SM00129, KISc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00411, KINESIN_MOTOR_1, 1 hit PS50067, KINESIN_MOTOR_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | KIF2C_HUMAN | |
Accessioni | Q99661Primary (citable) accession number: Q99661 Secondary accession number(s): B3ITR9 Q9BWV8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 11, 2001 |
Last sequence update: | January 23, 2002 | |
Last modified: | February 10, 2021 | |
This is version 207 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families