UniProtKB - Q99633 (PRP18_HUMAN)

BLAST

>sp|Q99633|PRP18_HUMAN Pre-mRNA-splicing factor 18 OS=Homo sapiens OX=9606 GN=PRPF18 PE=1 SV=1
MDILKSEILRKRQLVEDRNLLVENKKYFKRSELAKKEEEAYFERCGYKIQPKEEDQKPLT
SSNPVLELELAEEKLPMTLSRQEVIRRLRERGEPIRLFGETDYDAFQRLRKIEILTPEVN
KGLRNDLKAALDKIDQQYLNEIVGGQEPGEEDTQNDLKVHEENTTIEELEALGESLGKGD
DHKDMDIITKFLKFLLGVWAKELNAREDYVKRSVQGKLNSATQKQTESYLRPLFRKLRKR
NLPADIKESITDIIKFMLQREYVKANDAYLQMAIGNAPWPIGVTMVGIHARTGREKIFSK
HVAHVLNDETQRKYIQGLKRLMTICQKHFPTDPSKCVEYNAL

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History
Entry version 157 (12 Sep 2018)
Sequence version 1 (01 May 1997)
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Protein

Pre-mRNA-splicing factor 18

Gene

PRPF18

Organism

Homo sapiens (Human)

Status

Reviewed-Annotation score: -Experimental evidence at protein levelⁱ

Functionⁱ

Participates in the second step of pre-mRNA splicing.1 Publication

GO - Biological processⁱ

generation of catalytic spliceosome for second transesterification step Source: GO_Central
mRNA processing
Source: ProtInc
Traceable author statementⁱ
- 9000057
nuclear retention of unspliced pre-mRNA at the site of transcription Source: GO_Central
RNA splicing
Source: ProtInc
Traceable author statementⁱ
- 9000057

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Biological process	mRNA processing, mRNA splicing

Biological process

mRNA processing, mRNA splicing

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Pre-mRNA-splicing factor 18 Alternative name(s): PRP18 homolog Short name: hPRP18
Gene namesⁱ	Name:PRPF18 Synonyms:HPRP18
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 10

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000165630.13
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:17351 PRPF18
MIMⁱ	604993 gene
neXtProtⁱ	NX_Q99633

Keywords - Cellular componentⁱ

Nucleus, Spliceosome

Pathology & Biotechⁱ

Organism-specific databases

DisGeNET More...DisGeNETⁱ	8559
Open Targets More...OpenTargetsⁱ	ENSG00000165630
PharmGKBⁱ	PA38449

Polymorphism and mutation databases

BioMutaⁱ	PRPF18
DMDMⁱ	37082244

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000058582	1 – 342	Pre-mRNA-splicing factor 18Add BLAST		342

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Modified residueⁱ	1	N-acetylmethionineCombined sources		1

Keywords - PTMⁱ

Acetylation

Proteomic databases

EPDⁱ	Q99633
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q99633
PaxDbⁱ	Q99633
PeptideAtlas More...PeptideAtlasⁱ	Q99633
PRIDEⁱ	Q99633
ProteomicsDBⁱ	78369 78370 [Q99633-2]

PTM databases

iPTMnetⁱ	Q99633
PhosphoSitePlusⁱ	Q99633

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSG00000165630 Expressed in 95 organ(s), highest expression level in testis
CleanExⁱ	HS_PRPF18
ExpressionAtlasⁱ	Q99633 baseline and differential
Genevisibleⁱ	Q99633 HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	HPA037599 HPA047950

HPAⁱ

HPA037599
HPA047950

Interactionⁱ

Subunit structureⁱ

Heterodimer with PPIH. Interacts with PRPF4 and with the spliceosome. Part of a complex containing U4/U6 snRNPs.2 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
PPIH	O43447	4	EBI-2798416,EBI-1055615

With

Entry

#Exp.

IntAct

Notes

PPIH

O43447

EBI-2798416,EBI-1055615

Protein-protein interaction databases

BioGridⁱ	114129, 6 interactors
CORUMⁱ	Q99633
IntActⁱ	Q99633, 83 interactors
STRINGⁱ	9606.ENSP00000367835

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Beta strandⁱ	69 – 71	Combined sources	3
Helixⁱ	81 – 91	Combined sources	11
Helixⁱ	102 – 115	Combined sources	14
Beta strandⁱ	117 – 119	Combined sources	3

Show more details

3D structure databases

ProteinModelPortalⁱ	Q99633
SMRⁱ	Q99633
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ	Q99633

EvolutionaryTraceⁱ

Q99633

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the PRP18 family.Curated

Phylogenomic databases

eggNOGⁱ	KOG2808 Eukaryota ENOG410XR90 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000015073
HOGENOMⁱ	HOG000242389
HOVERGENⁱ	HBG053696
InParanoidⁱ	Q99633
KEGG Orthology (KO) More...KOⁱ	K12817
OMAⁱ	DICKHLL
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0ID9
PhylomeDBⁱ	Q99633
TreeFamⁱ	TF315049

Family and domain databases

Gene3Dⁱ	1.10.720.150, 1 hit
InterProⁱ	View protein in InterPro IPR004098 Prp18 IPR014906 PRP4-like IPR036285 PRP4-like_sf
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF02840 Prp18, 1 hit PF08799 PRP4, 1 hit
SMARTⁱ	View protein in SMART SM00500 SFM, 1 hit
SUPFAMⁱ	SSF158230 SSF158230, 1 hit

Sequences (2+)ⁱ

Sequence statusⁱ: Complete.

This entry describes 2 isoformsⁱ produced by alternative splicing. Align Add to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show all Align All

Isoform 1 (identifier: Q99633-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDILKSEILR KRQLVEDRNL LVENKKYFKR SELAKKEEEA YFERCGYKIQ 
        60         70         80         90        100
PKEEDQKPLT SSNPVLELEL AEEKLPMTLS RQEVIRRLRE RGEPIRLFGE 
       110        120        130        140        150
TDYDAFQRLR KIEILTPEVN KGLRNDLKAA LDKIDQQYLN EIVGGQEPGE 
       160        170        180        190        200
EDTQNDLKVH EENTTIEELE ALGESLGKGD DHKDMDIITK FLKFLLGVWA 
       210        220        230        240        250
KELNAREDYV KRSVQGKLNS ATQKQTESYL RPLFRKLRKR NLPADIKESI 
       260        270        280        290        300
TDIIKFMLQR EYVKANDAYL QMAIGNAPWP IGVTMVGIHA RTGREKIFSK 
       310        320        330        340 
HVAHVLNDET QRKYIQGLKR LMTICQKHFP TDPSKCVEYN AL

Length:342

Mass (Da):39,860

Last modified:May 1, 1997 - v1

Checksum:ⁱ269D79E1E733CF6D

Isoform 2 (identifier: Q99633-2) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
22-259: Missing.
286-342: Missing.

« Hide

        10         20         30         40 
MDILKSEILR KRQLVEDRNL LREYVKANDA YLQMAIGNAP WPIGVTM

Note: No experimental confirmation available.

Show »

Length:47

Mass (Da):5,474

Checksum:ⁱ3A4FB4FF31181C8A

Computationally mapped potential isoform sequencesⁱ

There are 2 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

Q5T9P7	Q5T9P7_HUMAN	Pre-mRNA-splicing factor 18 Pre-mRNA-splicing factor 18	PRPF18	249	Annotation score:
Q5T9P8	Q5T9P8_HUMAN	Pre-mRNA-splicing factor 18 Pre-mRNA-splicing factor 18	PRPF18	167	Annotation score:

Alternative sequence

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Alternative sequenceⁱVSP_008327	22 – 259	Missing in isoform 2. 1 PublicationAdd BLAST		238
Alternative sequenceⁱVSP_008328	286 – 342	Missing in isoform 2. 1 PublicationAdd BLAST		57

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U51990 mRNA Translation: AAB41490.1 AL157392 Genomic DNA No translation available. CH471072 Genomic DNA Translation: EAW86281.1 BC000794 mRNA Translation: AAH00794.1 BC002572 mRNA No translation available.
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS7100.1 [Q99633-1]
NCBI Reference Sequences More...RefSeqⁱ	NP_003666.1, NM_003675.3 [Q99633-1]
UniGeneⁱ	Hs.660649 Hs.729158

Genome annotation databases

Ensemblⁱ	ENST00000378572; ENSP00000367835; ENSG00000165630 [Q99633-1]
GeneIDⁱ	8559
KEGGⁱ	hsa:8559
UCSC genome browser More...UCSCⁱ	uc001imp.4 human [Q99633-1]

Keywords - Coding sequence diversityⁱ

Alternative splicing

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q99633	PRP18 pre-mRNA processing factor 18 homolog		PANTR		342	UniRef100_Q99633
PRP18-like protein		MACMU		342
PRP18-like protein		MACFA		342
Uncharacterized protein		PANPA		342
Uncharacterized protein		LOXAF		342
+3

Protein	Similar proteins		Species	Score	Length	Source
Q99633	Pre-mRNA-splicing factor 18		RAT		342	UniRef90_Q99633
Pre-mRNA-splicing factor 18		PONAB		342
Pre-mRNA-splicing factor 18		MOUSE		342
Pre-mRNA-splicing factor 18		BOVIN		342
PRP18 pre-mRNA processing factor 18 homolog		PANTR		342
+98

Protein	Similar proteins		Species	Score	Length	Source
Q99633	Pre-mRNA-splicing factor 18		RAT		342	UniRef50_Q99633
Pre-mRNA-splicing factor 18		MOUSE		342
Pre-mRNA-splicing factor 18		PONAB		342
Pre-mRNA-splicing factor 18		BOVIN		342
PRP18 pre-mRNA processing factor 18 homolog		PANTR		342
+266

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U51990 mRNA Translation: AAB41490.1 AL157392 Genomic DNA No translation available. CH471072 Genomic DNA Translation: EAW86281.1 BC000794 mRNA Translation: AAH00794.1 BC002572 mRNA No translation available.
CCDSⁱ	CCDS7100.1 [Q99633-1]
RefSeqⁱ	NP_003666.1, NM_003675.3 [Q99633-1]
UniGeneⁱ	Hs.660649 Hs.729158

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	2DK4	NMR	-	A	60-122	[»]
ProteinModelPortalⁱ	Q99633
SMRⁱ	Q99633
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	114129, 6 interactors
CORUMⁱ	Q99633
IntActⁱ	Q99633, 83 interactors
STRINGⁱ	9606.ENSP00000367835

PTM databases

iPTMnetⁱ	Q99633
PhosphoSitePlusⁱ	Q99633

Polymorphism and mutation databases

BioMutaⁱ	PRPF18
DMDMⁱ	37082244

Proteomic databases

EPDⁱ	Q99633
MaxQBⁱ	Q99633
PaxDbⁱ	Q99633
PeptideAtlasⁱ	Q99633
PRIDEⁱ	Q99633
ProteomicsDBⁱ	78369 78370 [Q99633-2]

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	8559
Structural Biology Knowledgebase	Search...

Genome annotation databases

Ensemblⁱ	ENST00000378572; ENSP00000367835; ENSG00000165630 [Q99633-1]
GeneIDⁱ	8559
KEGGⁱ	hsa:8559
UCSCⁱ	uc001imp.4 human [Q99633-1]

Organism-specific databases

CTDⁱ	8559
DisGeNETⁱ	8559
EuPathDBⁱ	HostDB:ENSG00000165630.13
GeneCardsⁱ	PRPF18
HGNCⁱ	HGNC:17351 PRPF18
HPAⁱ	HPA037599 HPA047950
MIMⁱ	604993 gene
neXtProtⁱ	NX_Q99633
OpenTargetsⁱ	ENSG00000165630
PharmGKBⁱ	PA38449
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	KOG2808 Eukaryota ENOG410XR90 LUCA
GeneTreeⁱ	ENSGT00390000015073
HOGENOMⁱ	HOG000242389
HOVERGENⁱ	HBG053696
InParanoidⁱ	Q99633
KOⁱ	K12817
OMAⁱ	DICKHLL
OrthoDBⁱ	EOG091G0ID9
PhylomeDBⁱ	Q99633
TreeFamⁱ	TF315049

Miscellaneous databases

ChiTaRSⁱ	PRPF18 human
EvolutionaryTraceⁱ	Q99633
GenomeRNAiⁱ	8559
Protein Ontology More...PROⁱ	PR:Q99633
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000165630 Expressed in 95 organ(s), highest expression level in testis
CleanExⁱ	HS_PRPF18
ExpressionAtlasⁱ	Q99633 baseline and differential
Genevisibleⁱ	Q99633 HS

Family and domain databases

Gene3Dⁱ	1.10.720.150, 1 hit
InterProⁱ	View protein in InterPro IPR004098 Prp18 IPR014906 PRP4-like IPR036285 PRP4-like_sf
Pfamⁱ	View protein in Pfam PF02840 Prp18, 1 hit PF08799 PRP4, 1 hit
SMARTⁱ	View protein in SMART SM00500 SFM, 1 hit
SUPFAMⁱ	SSF158230 SSF158230, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	PRP18_HUMAN
Accessionⁱ	Q99633Primary (citable) accession number: Q99633 Secondary accession number(s): Q5T9P9, Q9BUI9
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	September 26, 2003
	Last sequence update:	May 1, 1997
	Last modified:	September 12, 2018
	This is version 157 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
PDB cross-references
Index of Protein Data Bank (PDB) cross-references
Human chromosome 10
Human chromosome 10: entries, gene names and cross-references to MIM
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q99633 (PRP18_HUMAN)

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Pre-mRNA-splicing factor 18

PRPF18

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Nucleus

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Organism-specific databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Alternative sequence

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Polymorphism and mutation databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

UniProt is an ELIXIR core data resource

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Functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ