UniProtKB - Q99633 (PRP18_HUMAN)
Protein
Pre-mRNA-splicing factor 18
Gene
PRPF18
Organism
Homo sapiens (Human)
Status
Functioni
Participates in the second step of pre-mRNA splicing.1 Publication
GO - Biological processi
- generation of catalytic spliceosome for second transesterification step Source: GO_Central
- mRNA processing Source: ProtInc
- nuclear retention of unspliced pre-mRNA at the site of transcription Source: GO_Central
- RNA splicing Source: ProtInc
Keywordsi
Biological process | mRNA processing, mRNA splicing |
Enzyme and pathway databases
PathwayCommonsi | Q99633 |
Names & Taxonomyi
Protein namesi | Recommended name: Pre-mRNA-splicing factor 18Alternative name(s): PRP18 homolog Short name: hPRP18 |
Gene namesi | Name:PRPF18 Synonyms:HPRP18 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:17351, PRPF18 |
MIMi | 604993, gene |
neXtProti | NX_Q99633 |
VEuPathDBi | HostDB:ENSG00000165630.13 |
Subcellular locationi
Nucleus
- Nucleus speckle By similarity
Note: Colocalizes with spliceosomal snRNPs.By similarity
Nucleus
- nuclear speck Source: UniProtKB-SubCell
- nucleus Source: ProtInc
- spliceosomal complex Source: ProtInc
- U2-type post-spliceosomal complex Source: GO_Central
- U4/U6 x U5 tri-snRNP complex Source: GO_Central
- U5 snRNP Source: GO_Central
Keywords - Cellular componenti
Nucleus, SpliceosomePathology & Biotechi
Organism-specific databases
DisGeNETi | 8559 |
OpenTargetsi | ENSG00000165630 |
PharmGKBi | PA38449 |
Miscellaneous databases
Pharosi | Q99633, Tbio |
Genetic variation databases
BioMutai | PRPF18 |
DMDMi | 37082244 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000058582 | 1 – 342 | Pre-mRNA-splicing factor 18Add BLAST | 342 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineCombined sources | 1 |
Keywords - PTMi
AcetylationProteomic databases
EPDi | Q99633 |
jPOSTi | Q99633 |
MassIVEi | Q99633 |
MaxQBi | Q99633 |
PaxDbi | Q99633 |
PeptideAtlasi | Q99633 |
PRIDEi | Q99633 |
ProteomicsDBi | 78369 [Q99633-1] 78370 [Q99633-2] |
PTM databases
iPTMneti | Q99633 |
MetOSitei | Q99633 |
PhosphoSitePlusi | Q99633 |
Expressioni
Gene expression databases
Bgeei | ENSG00000165630, Expressed in testis and 121 other tissues |
ExpressionAtlasi | Q99633, baseline and differential |
Genevisiblei | Q99633, HS |
Organism-specific databases
HPAi | ENSG00000165630, Low tissue specificity |
Interactioni
Subunit structurei
Heterodimer with PPIH.
Interacts with PRPF4 and with the spliceosome.
Part of a complex containing U4/U6 snRNPs.
2 PublicationsBinary interactionsi
Q99633
Protein-protein interaction databases
BioGRIDi | 114129, 89 interactors |
CORUMi | Q99633 |
IntActi | Q99633, 89 interactors |
STRINGi | 9606.ENSP00000367835 |
Miscellaneous databases
RNActi | Q99633, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | Q99633 |
SMRi | Q99633 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q99633 |
Family & Domainsi
Sequence similaritiesi
Belongs to the PRP18 family.Curated
Phylogenomic databases
eggNOGi | KOG2808, Eukaryota |
GeneTreei | ENSGT00390000015073 |
HOGENOMi | CLU_039675_0_1_1 |
InParanoidi | Q99633 |
OMAi | DICKHLL |
OrthoDBi | 1485522at2759 |
PhylomeDBi | Q99633 |
TreeFami | TF315049 |
Family and domain databases
Gene3Di | 1.10.720.150, 1 hit |
IDEALi | IID00678 |
InterProi | View protein in InterPro IPR004098, Prp18 IPR014906, PRP4-like IPR036285, PRP4-like_sf IPR039979, PRPF18 |
PANTHERi | PTHR13007, PTHR13007, 1 hit |
Pfami | View protein in Pfam PF02840, Prp18, 1 hit PF08799, PRP4, 1 hit |
SMARTi | View protein in SMART SM00500, SFM, 1 hit |
SUPFAMi | SSF158230, SSF158230, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q99633-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MDILKSEILR KRQLVEDRNL LVENKKYFKR SELAKKEEEA YFERCGYKIQ
60 70 80 90 100
PKEEDQKPLT SSNPVLELEL AEEKLPMTLS RQEVIRRLRE RGEPIRLFGE
110 120 130 140 150
TDYDAFQRLR KIEILTPEVN KGLRNDLKAA LDKIDQQYLN EIVGGQEPGE
160 170 180 190 200
EDTQNDLKVH EENTTIEELE ALGESLGKGD DHKDMDIITK FLKFLLGVWA
210 220 230 240 250
KELNAREDYV KRSVQGKLNS ATQKQTESYL RPLFRKLRKR NLPADIKESI
260 270 280 290 300
TDIIKFMLQR EYVKANDAYL QMAIGNAPWP IGVTMVGIHA RTGREKIFSK
310 320 330 340
HVAHVLNDET QRKYIQGLKR LMTICQKHFP TDPSKCVEYN AL
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ5T9P7 | Q5T9P7_HUMAN | PRP18 homolog | PRPF18 | 249 | Annotation score: | ||
Q5T9P8 | Q5T9P8_HUMAN | Pre-mRNA-splicing factor 18 | PRPF18 | 167 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_008327 | 22 – 259 | Missing in isoform 2. 1 PublicationAdd BLAST | 238 | |
Alternative sequenceiVSP_008328 | 286 – 342 | Missing in isoform 2. 1 PublicationAdd BLAST | 57 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U51990 mRNA Translation: AAB41490.1 AL157392 Genomic DNA No translation available. CH471072 Genomic DNA Translation: EAW86281.1 BC000794 mRNA Translation: AAH00794.1 BC002572 mRNA No translation available. |
CCDSi | CCDS7100.1 [Q99633-1] |
RefSeqi | NP_003666.1, NM_003675.3 [Q99633-1] |
Genome annotation databases
Ensembli | ENST00000378572; ENSP00000367835; ENSG00000165630 [Q99633-1] |
GeneIDi | 8559 |
KEGGi | hsa:8559 |
UCSCi | uc001imp.4, human [Q99633-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U51990 mRNA Translation: AAB41490.1 AL157392 Genomic DNA No translation available. CH471072 Genomic DNA Translation: EAW86281.1 BC000794 mRNA Translation: AAH00794.1 BC002572 mRNA No translation available. |
CCDSi | CCDS7100.1 [Q99633-1] |
RefSeqi | NP_003666.1, NM_003675.3 [Q99633-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2DK4 | NMR | - | A | 60-122 | [»] | |
BMRBi | Q99633 | |||||
SMRi | Q99633 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 114129, 89 interactors |
CORUMi | Q99633 |
IntActi | Q99633, 89 interactors |
STRINGi | 9606.ENSP00000367835 |
PTM databases
iPTMneti | Q99633 |
MetOSitei | Q99633 |
PhosphoSitePlusi | Q99633 |
Genetic variation databases
BioMutai | PRPF18 |
DMDMi | 37082244 |
Proteomic databases
EPDi | Q99633 |
jPOSTi | Q99633 |
MassIVEi | Q99633 |
MaxQBi | Q99633 |
PaxDbi | Q99633 |
PeptideAtlasi | Q99633 |
PRIDEi | Q99633 |
ProteomicsDBi | 78369 [Q99633-1] 78370 [Q99633-2] |
Protocols and materials databases
Antibodypediai | 35190, 117 antibodies |
DNASUi | 8559 |
Genome annotation databases
Ensembli | ENST00000378572; ENSP00000367835; ENSG00000165630 [Q99633-1] |
GeneIDi | 8559 |
KEGGi | hsa:8559 |
UCSCi | uc001imp.4, human [Q99633-1] |
Organism-specific databases
CTDi | 8559 |
DisGeNETi | 8559 |
GeneCardsi | PRPF18 |
HGNCi | HGNC:17351, PRPF18 |
HPAi | ENSG00000165630, Low tissue specificity |
MIMi | 604993, gene |
neXtProti | NX_Q99633 |
OpenTargetsi | ENSG00000165630 |
PharmGKBi | PA38449 |
VEuPathDBi | HostDB:ENSG00000165630.13 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2808, Eukaryota |
GeneTreei | ENSGT00390000015073 |
HOGENOMi | CLU_039675_0_1_1 |
InParanoidi | Q99633 |
OMAi | DICKHLL |
OrthoDBi | 1485522at2759 |
PhylomeDBi | Q99633 |
TreeFami | TF315049 |
Enzyme and pathway databases
PathwayCommonsi | Q99633 |
Miscellaneous databases
BioGRID-ORCSi | 8559, 467 hits in 988 CRISPR screens |
ChiTaRSi | PRPF18, human |
EvolutionaryTracei | Q99633 |
GenomeRNAii | 8559 |
Pharosi | Q99633, Tbio |
PROi | PR:Q99633 |
RNActi | Q99633, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000165630, Expressed in testis and 121 other tissues |
ExpressionAtlasi | Q99633, baseline and differential |
Genevisiblei | Q99633, HS |
Family and domain databases
Gene3Di | 1.10.720.150, 1 hit |
IDEALi | IID00678 |
InterProi | View protein in InterPro IPR004098, Prp18 IPR014906, PRP4-like IPR036285, PRP4-like_sf IPR039979, PRPF18 |
PANTHERi | PTHR13007, PTHR13007, 1 hit |
Pfami | View protein in Pfam PF02840, Prp18, 1 hit PF08799, PRP4, 1 hit |
SMARTi | View protein in SMART SM00500, SFM, 1 hit |
SUPFAMi | SSF158230, SSF158230, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PRP18_HUMAN | |
Accessioni | Q99633Primary (citable) accession number: Q99633 Secondary accession number(s): Q5T9P9, Q9BUI9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 26, 2003 |
Last sequence update: | May 1, 1997 | |
Last modified: | April 7, 2021 | |
This is version 172 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 10
Human chromosome 10: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families