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Protein

SPRY domain-containing SOCS box protein 2

Gene

SPSB2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

  • ubiquitin-protein transferase activity Source: GO_Central

GO - Biological processi

Keywordsi

Biological processUbl conjugation pathway

Enzyme and pathway databases

ReactomeiR-HSA-8951664 Neddylation
R-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
SPRY domain-containing SOCS box protein 2
Short name:
SSB-2
Alternative name(s):
Gene-rich cluster protein C9
Gene namesi
Name:SPSB2
Synonyms:GRCC9, SSB2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiHostDB:ENSG00000111671.9
HGNCiHGNC:29522 SPSB2
MIMi611658 gene
neXtProtiNX_Q99619

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi116 – 119QTDH → HSVG: Enhances interaction with PAWR. 1 Publication4

Organism-specific databases

OpenTargetsiENSG00000111671
PharmGKBiPA142670872

Chemistry databases

ChEMBLiCHEMBL3325308

Polymorphism and mutation databases

BioMutaiSPSB2
DMDMi74732788

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002384741 – 263SPRY domain-containing SOCS box protein 2Add BLAST263

Proteomic databases

PaxDbiQ99619
PeptideAtlasiQ99619
PRIDEiQ99619
ProteomicsDBi78362

PTM databases

iPTMnetiQ99619
PhosphoSitePlusiQ99619

Expressioni

Gene expression databases

BgeeiENSG00000111671 Expressed in 161 organ(s), highest expression level in right lung
CleanExiHS_SPSB2
ExpressionAtlasiQ99619 baseline and differential
GenevisibleiQ99619 HS

Organism-specific databases

HPAiHPA055225

Interactioni

Subunit structurei

Component of the probable ECS(SPSB2) E3 ubiquitin-protein ligase complex which contains CUL5, RNF7/RBX2, Elongin BC complex and SPSB2 (PubMed:15601820). Interacts with CUL5, RNF7, ELOB and ELOC (PubMed:15601820). Interacts with MET (PubMed:15713673). Interacts (via B30.2/SPRY domain) with PAWR; this interaction occurs in association with the Elongin BC complex (PubMed:16498413, PubMed:20561531).4 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi124227, 16 interactors
ELMiQ99619
IntActiQ99619, 12 interactors
MINTiQ99619
STRINGi9606.ENSP00000428338

Chemistry databases

BindingDBiQ99619

Structurei

Secondary structure

1263
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ99619
SMRiQ99619
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ99619

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini26 – 221B30.2/SPRYPROSITE-ProRule annotationAdd BLAST196
Domaini222 – 263SOCS boxPROSITE-ProRule annotationAdd BLAST42

Domaini

The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes.By similarity

Sequence similaritiesi

Belongs to the SPSB family.Curated

Phylogenomic databases

eggNOGiKOG3953 Eukaryota
ENOG410XQC1 LUCA
GeneTreeiENSGT00390000009402
HOGENOMiHOG000230524
HOVERGENiHBG055793
InParanoidiQ99619
KOiK10344
OMAiCQVRISY
OrthoDBiEOG091G0DF0
PhylomeDBiQ99619
TreeFamiTF312822

Family and domain databases

CDDicd03719 SOCS_SSB2, 1 hit
InterProiView protein in InterPro
IPR001870 B30.2/SPRY
IPR013320 ConA-like_dom_sf
IPR001496 SOCS_box
IPR036036 SOCS_box-like_dom_sf
IPR003877 SPRY_dom
IPR037340 SSB2_SOCS
PfamiView protein in Pfam
PF07525 SOCS_box, 1 hit
PF00622 SPRY, 1 hit
SMARTiView protein in SMART
SM00253 SOCS, 1 hit
SM00969 SOCS_box, 1 hit
SM00449 SPRY, 1 hit
SUPFAMiSSF158235 SSF158235, 1 hit
SSF49899 SSF49899, 1 hit
PROSITEiView protein in PROSITE
PS50188 B302_SPRY, 1 hit
PS50225 SOCS, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q99619-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MGQTALAGGS SSTPTPQALY PDLSCPEGLE ELLSAPPPDL GAQRRHGWNP
60 70 80 90 100
KDCSENIEVK EGGLYFERRP VAQSTDGARG KRGYSRGLHA WEISWPLEQR
110 120 130 140 150
GTHAVVGVAT ALAPLQTDHY AALLGSNSES WGWDIGRGKL YHQSKGPGAP
160 170 180 190 200
QYPAGTQGEQ LEVPERLLVV LDMEEGTLGY AIGGTYLGPA FRGLKGRTLY
210 220 230 240 250
PAVSAVWGQC QVRIRYLGER RAEPHSLLHL SRLCVRHNLG DTRLGQVSAL
260
PLPPAMKRYL LYQ
Length:263
Mass (Da):28,630
Last modified:May 1, 1997 - v1
Checksum:i0729EDDD0B598665
GO
Isoform 2 (identifier: Q99619-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     222-263: AEPHSLLHLS...PAMKRYLLYQ → GEAWGRRGEN...PQPLFSAGKG

Note: No experimental confirmation available.
Show »
Length:267
Mass (Da):28,642
Checksum:i741ACE1245483D0E
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E5RIC2E5RIC2_HUMAN
SPRY domain-containing SOCS box pro...
SPSB2
82Annotation score:

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_057167222 – 263AEPHS…YLLYQ → GEAWGRRGENFLSLVAVVWD GNSSDKSRGDGPSSSLPQPL FSAGKG in isoform 2. 1 PublicationAdd BLAST42

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF403027 mRNA Translation: AAL57346.1
AK297963 mRNA Translation: BAH12697.1
U47924 Genomic DNA Translation: AAB51328.1
CH471116 Genomic DNA Translation: EAW88719.1
CH471116 Genomic DNA Translation: EAW88720.1
BC002983 mRNA Translation: AAH02983.1
BC071933 mRNA Translation: AAH71933.1
CCDSiCCDS8567.1 [Q99619-1]
RefSeqiNP_001139788.1, NM_001146316.1 [Q99619-1]
NP_001306599.1, NM_001319670.1 [Q99619-1]
NP_116030.1, NM_032641.3 [Q99619-1]
UniGeneiHs.479856

Genome annotation databases

EnsembliENST00000519357; ENSP00000431037; ENSG00000111671 [Q99619-2]
ENST00000523102; ENSP00000430872; ENSG00000111671 [Q99619-1]
ENST00000524270; ENSP00000428338; ENSG00000111671 [Q99619-1]
GeneIDi84727
KEGGihsa:84727
UCSCiuc001qrl.4 human [Q99619-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF403027 mRNA Translation: AAL57346.1
AK297963 mRNA Translation: BAH12697.1
U47924 Genomic DNA Translation: AAB51328.1
CH471116 Genomic DNA Translation: EAW88719.1
CH471116 Genomic DNA Translation: EAW88720.1
BC002983 mRNA Translation: AAH02983.1
BC071933 mRNA Translation: AAH71933.1
CCDSiCCDS8567.1 [Q99619-1]
RefSeqiNP_001139788.1, NM_001146316.1 [Q99619-1]
NP_001306599.1, NM_001319670.1 [Q99619-1]
NP_116030.1, NM_032641.3 [Q99619-1]
UniGeneiHs.479856

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3EMWX-ray1.80A26-219[»]
5XN3X-ray1.34A22-220[»]
ProteinModelPortaliQ99619
SMRiQ99619
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi124227, 16 interactors
ELMiQ99619
IntActiQ99619, 12 interactors
MINTiQ99619
STRINGi9606.ENSP00000428338

Chemistry databases

BindingDBiQ99619
ChEMBLiCHEMBL3325308

PTM databases

iPTMnetiQ99619
PhosphoSitePlusiQ99619

Polymorphism and mutation databases

BioMutaiSPSB2
DMDMi74732788

Proteomic databases

PaxDbiQ99619
PeptideAtlasiQ99619
PRIDEiQ99619
ProteomicsDBi78362

Protocols and materials databases

DNASUi84727
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000519357; ENSP00000431037; ENSG00000111671 [Q99619-2]
ENST00000523102; ENSP00000430872; ENSG00000111671 [Q99619-1]
ENST00000524270; ENSP00000428338; ENSG00000111671 [Q99619-1]
GeneIDi84727
KEGGihsa:84727
UCSCiuc001qrl.4 human [Q99619-1]

Organism-specific databases

CTDi84727
EuPathDBiHostDB:ENSG00000111671.9
GeneCardsiSPSB2
HGNCiHGNC:29522 SPSB2
HPAiHPA055225
MIMi611658 gene
neXtProtiNX_Q99619
OpenTargetsiENSG00000111671
PharmGKBiPA142670872
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3953 Eukaryota
ENOG410XQC1 LUCA
GeneTreeiENSGT00390000009402
HOGENOMiHOG000230524
HOVERGENiHBG055793
InParanoidiQ99619
KOiK10344
OMAiCQVRISY
OrthoDBiEOG091G0DF0
PhylomeDBiQ99619
TreeFamiTF312822

Enzyme and pathway databases

UniPathwayi
UPA00143

ReactomeiR-HSA-8951664 Neddylation
R-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation

Miscellaneous databases

EvolutionaryTraceiQ99619
GenomeRNAii84727
PROiPR:Q99619
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000111671 Expressed in 161 organ(s), highest expression level in right lung
CleanExiHS_SPSB2
ExpressionAtlasiQ99619 baseline and differential
GenevisibleiQ99619 HS

Family and domain databases

CDDicd03719 SOCS_SSB2, 1 hit
InterProiView protein in InterPro
IPR001870 B30.2/SPRY
IPR013320 ConA-like_dom_sf
IPR001496 SOCS_box
IPR036036 SOCS_box-like_dom_sf
IPR003877 SPRY_dom
IPR037340 SSB2_SOCS
PfamiView protein in Pfam
PF07525 SOCS_box, 1 hit
PF00622 SPRY, 1 hit
SMARTiView protein in SMART
SM00253 SOCS, 1 hit
SM00969 SOCS_box, 1 hit
SM00449 SPRY, 1 hit
SUPFAMiSSF158235 SSF158235, 1 hit
SSF49899 SSF49899, 1 hit
PROSITEiView protein in PROSITE
PS50188 B302_SPRY, 1 hit
PS50225 SOCS, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSPSB2_HUMAN
AccessioniPrimary (citable) accession number: Q99619
Secondary accession number(s): B7Z4W1, D3DUT0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: May 1, 1997
Last modified: November 7, 2018
This is version 154 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
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