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Protein

Eukaryotic translation initiation factor 3 subunit C

Gene

EIF3C

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773, PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773).UniRule annotation3 Publications

GO - Molecular functioni

  • RNA binding Source: UniProtKB
  • translation initiation factor activity Source: UniProtKB-UniRule
  • translation initiation factor binding Source: GO_Central

GO - Biological processi

  • formation of cytoplasmic translation initiation complex Source: UniProtKB-UniRule
  • positive regulation of mRNA binding Source: ParkinsonsUK-UCL
  • positive regulation of translation Source: ParkinsonsUK-UCL
  • translational initiation Source: UniProtKB

Keywordsi

Molecular functionInitiation factor
Biological processProtein biosynthesis

Enzyme and pathway databases

ReactomeiR-HSA-156827 L13a-mediated translational silencing of Ceruloplasmin expression
R-HSA-72649 Translation initiation complex formation
R-HSA-72689 Formation of a pool of free 40S subunits
R-HSA-72695 Formation of the ternary complex, and subsequently, the 43S complex
R-HSA-72702 Ribosomal scanning and start codon recognition
R-HSA-72706 GTP hydrolysis and joining of the 60S ribosomal subunit

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 3 subunit CUniRule annotation
Short name:
eIF3cUniRule annotation
Alternative name(s):
Eukaryotic translation initiation factor 3 subunit 8UniRule annotation
eIF3 p110UniRule annotation
Gene namesi
Name:EIF3CUniRule annotation
Synonyms:EIF3S8UniRule annotation
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

EuPathDBiHostDB:ENSG00000184110.14
HGNCiHGNC:3279 EIF3C
MIMi603916 gene
neXtProtiNX_Q99613

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi8663
OpenTargetsiENSG00000184110
PharmGKBiPA162384646

Polymorphism and mutation databases

BioMutaiEIF3C
DMDMi6685539

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001235251 – 913Eukaryotic translation initiation factor 3 subunit CAdd BLAST913

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei9PhosphoserineUniRule annotation1 Publication1
Modified residuei11PhosphoserineUniRule annotation1 Publication1
Modified residuei13PhosphoserineUniRule annotation1 Publication1
Modified residuei15PhosphoserineUniRule annotation1 Publication1
Modified residuei16PhosphoserineUniRule annotation1 Publication1
Modified residuei18PhosphoserineUniRule annotation1 Publication1
Modified residuei39PhosphoserineUniRule annotation1 Publication1
Modified residuei99N6-acetyllysineBy similarity1
Modified residuei166PhosphoserineUniRule annotation1 Publication1
Modified residuei178PhosphoserineBy similarity1
Modified residuei181PhosphoserineBy similarity1
Modified residuei182PhosphoserineBy similarity1
Modified residuei524PhosphothreonineUniRule annotation1 Publication1
Modified residuei643N6-acetyllysineBy similarity1
Modified residuei909PhosphoserineUniRule annotation1 Publication1

Post-translational modificationi

Phosphorylated. Phosphorylation is enhanced upon serum stimulation.UniRule annotation1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ99613
MaxQBiQ99613
PaxDbiQ99613
PeptideAtlasiQ99613
PRIDEiQ99613
ProteomicsDBi78357

PTM databases

iPTMnetiQ99613
PhosphoSitePlusiQ99613
SwissPalmiQ99613

Expressioni

Gene expression databases

BgeeiENSG00000184110 Expressed in 167 organ(s), highest expression level in right testis
CleanExiHS_EIF3C
HS_EIF3CL
ExpressionAtlasiQ99613 baseline and differential
GenevisibleiQ99613 HS

Organism-specific databases

HPAiCAB034045
HPA047097
HPA049495
HPA050112

Interactioni

Subunit structurei

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3. Identified in a HCV IRES-mediated translation complex, at least composed of EIF3C, IGF2BP1, RPS3 and HCV RNA-replicon. Interacts with ALKBH4, IFIT1 and IFIT2.UniRule annotation11 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi114212, 117 interactors
CORUMiQ99613
DIPiDIP-32865N
IntActiQ99613, 52 interactors
MINTiQ99613
STRINGi9606.ENSP00000332604

Structurei

Secondary structure

1913
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ99613
SMRiQ99613
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini673 – 849PCIPROSITE-ProRule annotationAdd BLAST177

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi164 – 189Asp/Glu-rich (acidic)Add BLAST26
Compositional biasi243 – 246Poly-Glu4
Compositional biasi291 – 294Poly-Glu4

Sequence similaritiesi

Belongs to the eIF-3 subunit C family.UniRule annotation

Phylogenomic databases

eggNOGiKOG1076 Eukaryota
ENOG410XRU3 LUCA
GeneTreeiENSGT00390000017900
HOGENOMiHOG000029414
HOVERGENiHBG035174
InParanoidiQ99613
KOiK03252
OMAiVVMHRSE
OrthoDBiEOG091G02A3
PhylomeDBiQ99613
TreeFamiTF101520

Family and domain databases

Gene3Di1.10.10.10, 1 hit
HAMAPiMF_03002 eIF3c, 1 hit
InterProiView protein in InterPro
IPR027516 EIF3C
IPR008905 EIF3C_N_dom
IPR000717 PCI_dom
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR13937 PTHR13937, 1 hit
PfamiView protein in Pfam
PF05470 eIF-3c_N, 1 hit
PF01399 PCI, 1 hit
SMARTiView protein in SMART
SM00088 PINT, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
PROSITEiView protein in PROSITE
PS50250 PCI, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q99613-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSRFFTTGSD SESESSLSGE ELVTKPVGGN YGKQPLLLSE DEEDTKRVVR
60 70 80 90 100
SAKDKRFEEL TNLIRTIRNA MKIRDVTKCL EEFELLGKAY GKAKSIVDKE
110 120 130 140 150
GVPRFYIRIL ADLEDYLNEL WEDKEGKKKM NKNNAKALST LRQKIRKYNR
160 170 180 190 200
DFESHITSYK QNPEQSADED AEKNEEDSEG SSDEDEDEDG VSAATFLKKK
210 220 230 240 250
SEAPSGESRK FLKKMDDEDE DSEDSEDDED WDTGSTSSDS DSEEEEGKQT
260 270 280 290 300
ALASRFLKKA PTTDEDKKAA EKKREDKAKK KHDRKSKRLD EEEEDNEGGE
310 320 330 340 350
WERVRGGVPL VKEKPKMFAK GTEITHAVVI KKLNEILQAR GKKGTDRAAQ
360 370 380 390 400
IELLQLLVQI AAENNLGEGV IVKIKFNIIA SLYDYNPNLA TYMKPEMWGK
410 420 430 440 450
CLDCINELMD ILFANPNIFV GENILEESEN LHNADQPLRV RGCILTLVER
460 470 480 490 500
MDEEFTKIMQ NTDPHSQEYV EHLKDEAQVC AIIERVQRYL EEKGTTEEVC
510 520 530 540 550
RIYLLRILHT YYKFDYKAHQ RQLTPPEGSS KSEQDQAENE GEDSAVLMER
560 570 580 590 600
LCKYIYAKDR TDRIRTCAIL CHIYHHALHS RWYQARDLML MSHLQDNIQH
610 620 630 640 650
ADPPVQILYN RTMVQLGICA FRQGLTKDAH NALLDIQSSG RAKELLGQGL
660 670 680 690 700
LLRSLQERNQ EQEKVERRRQ VPFHLHINLE LLECVYLVSA MLLEIPYMAA
710 720 730 740 750
HESDARRRMI SKQFHHQLRV GERQPLLGPP ESMREHVVAA SKAMKMGDWK
760 770 780 790 800
TCHSFIINEK MNGKVWDLFP EADKVRTMLV RKIQEESLRT YLFTYSSVYD
810 820 830 840 850
SISMETLSDM FELDLPTVHS IISKMIINEE LMASLDQPTQ TVVMHRTEPT
860 870 880 890 900
AQQNLALQLA EKLGSLVENN ERVFDHKQGT YGGYFRDQKD GYRKNEGYMR
910
RGGYRQQQSQ TAY
Length:913
Mass (Da):105,344
Last modified:May 1, 1997 - v1
Checksum:iCE5029F4EB51C1AA
GO
Isoform 2 (identifier: Q99613-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     120-129: Missing.

Note: No experimental confirmation available.
Show »
Length:903
Mass (Da):104,101
Checksum:iF877B86AD029CD67
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H3BPE4H3BPE4_HUMAN
Eukaryotic translation initiation f...
EIF3C
148Annotation score:
H3BPE3H3BPE3_HUMAN
Eukaryotic translation initiation f...
EIF3C
132Annotation score:
H3BTY8H3BTY8_HUMAN
Eukaryotic translation initiation f...
EIF3C
79Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti313 – 314EK → VR in AAC27674 (PubMed:10493829).Curated2
Sequence conflicti737V → A in BAG64747 (PubMed:14702039).Curated1

Mass spectrometryi

Molecular mass is 106143.8 Da from positions 1 - 913. 1 Publication
Molecular mass is 106855±40 Da from positions 1 - 913. Determined by MALDI. 1 Publication

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_055472120 – 129Missing in isoform 2. 1 Publication10

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U46025 Genomic DNA Translation: AAD03462.1
AC002544 Genomic DNA Translation: AAC27426.1
U91326 Genomic DNA Translation: AAC27674.1
AK000739 mRNA Translation: BAA91352.1
AK292155 mRNA Translation: BAF84844.1
AK303790 mRNA Translation: BAG64747.1
AC145285 Genomic DNA No translation available.
BC000533 mRNA Translation: AAH00533.1
BC001571 mRNA Translation: AAH01571.1
BC071705 mRNA Translation: AAH71705.1
BC157842 mRNA Translation: AAI57843.1
BC157849 mRNA Translation: AAI57850.1
CH878380 Genomic DNA Translation: EAW50492.1
BT007335 mRNA Translation: AAP35999.1
CCDSiCCDS10638.1 [Q99613-1]
CCDS66993.1 [Q99613-2]
RefSeqiNP_001032897.1, NM_001037808.2 [Q99613-1]
NP_001186071.1, NM_001199142.1 [Q99613-1]
NP_001254503.1, NM_001267574.2 [Q99613-1]
NP_001273407.1, NM_001286478.1 [Q99613-2]
NP_003743.1, NM_003752.4 [Q99613-1]
UniGeneiHs.567374

Genome annotation databases

EnsembliENST00000331666; ENSP00000332604; ENSG00000184110 [Q99613-1]
ENST00000395587; ENSP00000378953; ENSG00000184110 [Q99613-1]
ENST00000564243; ENSP00000456416; ENSG00000184110 [Q99613-2]
ENST00000566501; ENSP00000457963; ENSG00000184110 [Q99613-1]
ENST00000566866; ENSP00000457418; ENSG00000184110 [Q99613-1]
GeneIDi8663
KEGGihsa:8663
UCSCiuc002dqs.6 human [Q99613-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U46025 Genomic DNA Translation: AAD03462.1
AC002544 Genomic DNA Translation: AAC27426.1
U91326 Genomic DNA Translation: AAC27674.1
AK000739 mRNA Translation: BAA91352.1
AK292155 mRNA Translation: BAF84844.1
AK303790 mRNA Translation: BAG64747.1
AC145285 Genomic DNA No translation available.
BC000533 mRNA Translation: AAH00533.1
BC001571 mRNA Translation: AAH01571.1
BC071705 mRNA Translation: AAH71705.1
BC157842 mRNA Translation: AAI57843.1
BC157849 mRNA Translation: AAI57850.1
CH878380 Genomic DNA Translation: EAW50492.1
BT007335 mRNA Translation: AAP35999.1
CCDSiCCDS10638.1 [Q99613-1]
CCDS66993.1 [Q99613-2]
RefSeqiNP_001032897.1, NM_001037808.2 [Q99613-1]
NP_001186071.1, NM_001199142.1 [Q99613-1]
NP_001254503.1, NM_001267574.2 [Q99613-1]
NP_001273407.1, NM_001286478.1 [Q99613-2]
NP_003743.1, NM_003752.4 [Q99613-1]
UniGeneiHs.567374

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3J8Belectron microscopy-C326-846[»]
3J8Celectron microscopy-C326-846[»]
ProteinModelPortaliQ99613
SMRiQ99613
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114212, 117 interactors
CORUMiQ99613
DIPiDIP-32865N
IntActiQ99613, 52 interactors
MINTiQ99613
STRINGi9606.ENSP00000332604

PTM databases

iPTMnetiQ99613
PhosphoSitePlusiQ99613
SwissPalmiQ99613

Polymorphism and mutation databases

BioMutaiEIF3C
DMDMi6685539

Proteomic databases

EPDiQ99613
MaxQBiQ99613
PaxDbiQ99613
PeptideAtlasiQ99613
PRIDEiQ99613
ProteomicsDBi78357

Protocols and materials databases

DNASUi8663
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000331666; ENSP00000332604; ENSG00000184110 [Q99613-1]
ENST00000395587; ENSP00000378953; ENSG00000184110 [Q99613-1]
ENST00000564243; ENSP00000456416; ENSG00000184110 [Q99613-2]
ENST00000566501; ENSP00000457963; ENSG00000184110 [Q99613-1]
ENST00000566866; ENSP00000457418; ENSG00000184110 [Q99613-1]
GeneIDi8663
KEGGihsa:8663
UCSCiuc002dqs.6 human [Q99613-1]

Organism-specific databases

CTDi8663
DisGeNETi8663
EuPathDBiHostDB:ENSG00000184110.14
GeneCardsiEIF3C
H-InvDBiHIX0038592
HGNCiHGNC:3279 EIF3C
HPAiCAB034045
HPA047097
HPA049495
HPA050112
MIMi603916 gene
neXtProtiNX_Q99613
OpenTargetsiENSG00000184110
PharmGKBiPA162384646
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1076 Eukaryota
ENOG410XRU3 LUCA
GeneTreeiENSGT00390000017900
HOGENOMiHOG000029414
HOVERGENiHBG035174
InParanoidiQ99613
KOiK03252
OMAiVVMHRSE
OrthoDBiEOG091G02A3
PhylomeDBiQ99613
TreeFamiTF101520

Enzyme and pathway databases

ReactomeiR-HSA-156827 L13a-mediated translational silencing of Ceruloplasmin expression
R-HSA-72649 Translation initiation complex formation
R-HSA-72689 Formation of a pool of free 40S subunits
R-HSA-72695 Formation of the ternary complex, and subsequently, the 43S complex
R-HSA-72702 Ribosomal scanning and start codon recognition
R-HSA-72706 GTP hydrolysis and joining of the 60S ribosomal subunit

Miscellaneous databases

ChiTaRSiEIF3C human
GeneWikiiEIF3C
GenomeRNAii8663
PROiPR:Q99613
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000184110 Expressed in 167 organ(s), highest expression level in right testis
CleanExiHS_EIF3C
HS_EIF3CL
ExpressionAtlasiQ99613 baseline and differential
GenevisibleiQ99613 HS

Family and domain databases

Gene3Di1.10.10.10, 1 hit
HAMAPiMF_03002 eIF3c, 1 hit
InterProiView protein in InterPro
IPR027516 EIF3C
IPR008905 EIF3C_N_dom
IPR000717 PCI_dom
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR13937 PTHR13937, 1 hit
PfamiView protein in Pfam
PF05470 eIF-3c_N, 1 hit
PF01399 PCI, 1 hit
SMARTiView protein in SMART
SM00088 PINT, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
PROSITEiView protein in PROSITE
PS50250 PCI, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiEIF3C_HUMAN
AccessioniPrimary (citable) accession number: Q99613
Secondary accession number(s): A8K7Z0
, B2RXG3, B4E1D5, H3BRV0, O00215, Q9BW98
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1997
Last modified: November 7, 2018
This is version 190 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  3. Translation initiation factors
    List of translation initiation factor entries
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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