UniProtKB - Q99608 (NECD_HUMAN)
Protein
Necdin
Gene
NDN
Organism
Homo sapiens (Human)
Status
Functioni
Growth suppressor that facilitates the entry of the cell into cell cycle arrest. Functionally similar to the retinoblastoma protein it binds to and represses the activity of cell-cycle-promoting proteins such as SV40 large T antigen, adenovirus E1A, and the transcription factor E2F. Necdin also interacts with p53 and works in an additive manner to inhibit cell growth. Functions also as transcription factor and binds directly to specific guanosine-rich DNA sequences (By similarity).By similarity
Miscellaneous
Located in the Prader-Willi syndrome (PWS) chromosome region. Prader-Willi syndrome is a contiguous gene syndrome resulting from deletion of the paternal copies of the imprinted SNRPN gene, the necdin gene, and possibly other genes within the chromosome region 15q11-q13.
GO - Molecular functioni
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: Ensembl
- gamma-tubulin binding Source: Ensembl
- promoter-specific chromatin binding Source: Ensembl
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: Ensembl
GO - Biological processi
- axonal fasciculation Source: Ensembl
- axon extension Source: Ensembl
- central nervous system development Source: Ensembl
- cytokine-mediated signaling pathway Source: Reactome
- genetic imprinting Source: Ensembl
- glial cell migration Source: Ensembl
- multicellular organismal homeostasis Source: Ensembl
- negative regulation of cell population proliferation Source: InterPro
- negative regulation of DNA-binding transcription factor activity Source: Ensembl
- negative regulation of transcription by RNA polymerase II Source: Ensembl
- neuron migration Source: Ensembl
- neurotrophin TRK receptor signaling pathway Source: Ensembl
- positive regulation of protein deacetylation Source: Ensembl
- post-embryonic development Source: Ensembl
- regulation of growth Source: UniProtKB-KW
- respiratory system process Source: Ensembl
- sensory perception of pain Source: Ensembl
Keywordsi
Molecular function | DNA-binding |
Biological process | Growth regulation, Transcription, Transcription regulation |
Enzyme and pathway databases
PathwayCommonsi | Q99608 |
Reactomei | R-HSA-6785807, Interleukin-4 and Interleukin-13 signaling |
SIGNORi | Q99608 |
Names & Taxonomyi
Protein namesi | Recommended name: Necdin |
Gene namesi | Name:NDN |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:7675, NDN |
MIMi | 602117, gene |
neXtProti | NX_Q99608 |
VEuPathDBi | HostDB:ENSG00000182636.5 |
Subcellular locationi
Nucleus
Other locations
Note: Neural perikarya, translocates to the nucleus of postmitotic neurons and interacts with the nuclear matrix.
Cytoskeleton
- centrosome Source: Ensembl
Cytosol
- cytosol Source: HPA
Nucleus
- nucleoplasm Source: HPA
Other locations
- cell projection Source: Ensembl
- perikaryon Source: UniProtKB-SubCell
- protein-containing complex Source: Ensembl
Keywords - Cellular componenti
NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 4692 |
GeneReviewsi | NDN |
MalaCardsi | NDN |
MIMi | 176270, phenotype |
OpenTargetsi | ENSG00000182636 |
Orphaneti | 177910, Prader-Willi syndrome due to imprinting mutation 98754, Prader-Willi syndrome due to maternal uniparental disomy of chromosome 15 177901, Prader-Willi syndrome due to paternal deletion of 15q11q13 type 1 177904, Prader-Willi syndrome due to paternal deletion of 15q11q13 type 2 |
PharmGKBi | PA31479 |
Miscellaneous databases
Pharosi | Q99608, Tbio |
Genetic variation databases
BioMutai | NDN |
DMDMi | 17380142 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000156740 | 1 – 321 | NecdinAdd BLAST | 321 |
Proteomic databases
EPDi | Q99608 |
jPOSTi | Q99608 |
MassIVEi | Q99608 |
PaxDbi | Q99608 |
PeptideAtlasi | Q99608 |
PRIDEi | Q99608 |
ProteomicsDBi | 78353 |
PTM databases
iPTMneti | Q99608 |
PhosphoSitePlusi | Q99608 |
Expressioni
Tissue specificityi
Almost ubiquitous. Detected in fetal brain, lung, liver and kidney; in adult heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine and colon. Not detected in peripheral blood leukocytes. In brain, restricted to post-mitotic neurons.
Gene expression databases
Bgeei | ENSG00000182636, Expressed in metanephros and 240 other tissues |
ExpressionAtlasi | Q99608, baseline and differential |
Genevisiblei | Q99608, HS |
Organism-specific databases
HPAi | ENSG00000182636, Low tissue specificity |
Interactioni
Subunit structurei
Binds to the transactivation domains of E2F1 and p53. Binds also SV40 large T antigen and adenovirus E1A.
Interacts with nucleobindin 1 and 2 (By similarity).
By similarityBinary interactionsi
Hide detailsQ99608
GO - Molecular functioni
- gamma-tubulin binding Source: Ensembl
Protein-protein interaction databases
BioGRIDi | 110772, 384 interactors |
IntActi | Q99608, 39 interactors |
STRINGi | 9606.ENSP00000332643 |
Miscellaneous databases
RNActi | Q99608, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 98 – 297 | MAGEPROSITE-ProRule annotationAdd BLAST | 200 |
Phylogenomic databases
eggNOGi | KOG4562, Eukaryota |
GeneTreei | ENSGT00940000163084 |
HOGENOMi | CLU_039582_1_1_1 |
InParanoidi | Q99608 |
OMAi | RMIIWFP |
OrthoDBi | 1195799at2759 |
PhylomeDBi | Q99608 |
TreeFami | TF328505 |
Family and domain databases
Gene3Di | 1.10.10.1200, 1 hit 1.10.10.1210, 1 hit |
InterProi | View protein in InterPro IPR037445, MAGE IPR041898, MAGE_WH1 IPR041899, MAGE_WH2 IPR002190, MHD_dom IPR030086, Necdin |
PANTHERi | PTHR11736, PTHR11736, 1 hit PTHR11736:SF10, PTHR11736:SF10, 1 hit |
Pfami | View protein in Pfam PF01454, MAGE, 1 hit |
SMARTi | View protein in SMART SM01373, MAGE, 1 hit |
PROSITEi | View protein in PROSITE PS50838, MAGE, 1 hit |
i Sequence
Sequence statusi: Complete.
Q99608-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSEQSKDLSD PNFAAEAPNS EVHSSPGVSE GVPPSATLAE PQSPPLGPTA
60 70 80 90 100
APQAAPPPQA PNDEGDPKAL QQAAEEGRAH QAPSAAQPGP APPAPAQLVQ
110 120 130 140 150
KAHELMWYVL VKDQKKMIIW FPDMVKDVIG SYKKWCRSIL RRTSLILARV
160 170 180 190 200
FGLHLRLTSL HTMEFALVKA LEPEELDRVA LSNRMPMTGL LLMILSLIYV
210 220 230 240 250
KGRGARESAV WNVLRILGLR PWKKHSTFGD VRKLITEEFV QMNYLKYQRV
260 270 280 290 300
PYVEPPEYEF FWGSRASREI TKMQIMEFLA RVFKKDPQAW PSRYREALEE
310 320
ARALREANPT AHYPRSSVSE D
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_065889 | 318 | V → A1 PublicationCorresponds to variant dbSNP:rs114077338EnsemblClinVar. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U35139 mRNA Translation: AAB39469.1 AB007828 Genomic DNA Translation: BAA22660.1 AK312779 mRNA Translation: BAG35642.1 BC008750 mRNA Translation: AAH08750.1 |
CCDSi | CCDS10014.1 |
RefSeqi | NP_002478.1, NM_002487.2 |
Genome annotation databases
Ensembli | ENST00000649030; ENSP00000497916; ENSG00000182636 |
GeneIDi | 4692 |
KEGGi | hsa:4692 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U35139 mRNA Translation: AAB39469.1 AB007828 Genomic DNA Translation: BAA22660.1 AK312779 mRNA Translation: BAG35642.1 BC008750 mRNA Translation: AAH08750.1 |
CCDSi | CCDS10014.1 |
RefSeqi | NP_002478.1, NM_002487.2 |
3D structure databases
SMRi | Q99608 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 110772, 384 interactors |
IntActi | Q99608, 39 interactors |
STRINGi | 9606.ENSP00000332643 |
PTM databases
iPTMneti | Q99608 |
PhosphoSitePlusi | Q99608 |
Genetic variation databases
BioMutai | NDN |
DMDMi | 17380142 |
Proteomic databases
EPDi | Q99608 |
jPOSTi | Q99608 |
MassIVEi | Q99608 |
PaxDbi | Q99608 |
PeptideAtlasi | Q99608 |
PRIDEi | Q99608 |
ProteomicsDBi | 78353 |
Protocols and materials databases
Antibodypediai | 22276, 356 antibodies |
DNASUi | 4692 |
Genome annotation databases
Ensembli | ENST00000649030; ENSP00000497916; ENSG00000182636 |
GeneIDi | 4692 |
KEGGi | hsa:4692 |
Organism-specific databases
CTDi | 4692 |
DisGeNETi | 4692 |
GeneCardsi | NDN |
GeneReviewsi | NDN |
HGNCi | HGNC:7675, NDN |
HPAi | ENSG00000182636, Low tissue specificity |
MalaCardsi | NDN |
MIMi | 176270, phenotype 602117, gene |
neXtProti | NX_Q99608 |
OpenTargetsi | ENSG00000182636 |
Orphaneti | 177910, Prader-Willi syndrome due to imprinting mutation 98754, Prader-Willi syndrome due to maternal uniparental disomy of chromosome 15 177901, Prader-Willi syndrome due to paternal deletion of 15q11q13 type 1 177904, Prader-Willi syndrome due to paternal deletion of 15q11q13 type 2 |
PharmGKBi | PA31479 |
VEuPathDBi | HostDB:ENSG00000182636.5 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG4562, Eukaryota |
GeneTreei | ENSGT00940000163084 |
HOGENOMi | CLU_039582_1_1_1 |
InParanoidi | Q99608 |
OMAi | RMIIWFP |
OrthoDBi | 1195799at2759 |
PhylomeDBi | Q99608 |
TreeFami | TF328505 |
Enzyme and pathway databases
PathwayCommonsi | Q99608 |
Reactomei | R-HSA-6785807, Interleukin-4 and Interleukin-13 signaling |
SIGNORi | Q99608 |
Miscellaneous databases
BioGRID-ORCSi | 4692, 6 hits in 997 CRISPR screens |
ChiTaRSi | NDN, human |
GeneWikii | NDN_(gene) |
GenomeRNAii | 4692 |
Pharosi | Q99608, Tbio |
PROi | PR:Q99608 |
RNActi | Q99608, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000182636, Expressed in metanephros and 240 other tissues |
ExpressionAtlasi | Q99608, baseline and differential |
Genevisiblei | Q99608, HS |
Family and domain databases
Gene3Di | 1.10.10.1200, 1 hit 1.10.10.1210, 1 hit |
InterProi | View protein in InterPro IPR037445, MAGE IPR041898, MAGE_WH1 IPR041899, MAGE_WH2 IPR002190, MHD_dom IPR030086, Necdin |
PANTHERi | PTHR11736, PTHR11736, 1 hit PTHR11736:SF10, PTHR11736:SF10, 1 hit |
Pfami | View protein in Pfam PF01454, MAGE, 1 hit |
SMARTi | View protein in SMART SM01373, MAGE, 1 hit |
PROSITEi | View protein in PROSITE PS50838, MAGE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NECD_HUMAN | |
Accessioni | Q99608Primary (citable) accession number: Q99608 Secondary accession number(s): B2R6Z5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 18, 2001 |
Last sequence update: | May 1, 1997 | |
Last modified: | April 7, 2021 | |
This is version 169 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 15
Human chromosome 15: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot