Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

P2X purinoceptor 4

Gene

P2RX4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Receptor for ATP that acts as a ligand-gated ion channel. This receptor is insensitive to the antagonists PPADS and suramin.3 Publications

GO - Molecular functioni

  • ATP binding Source: BHF-UCL
  • cadherin binding Source: BHF-UCL
  • copper ion binding Source: BHF-UCL
  • extracellularly ATP-gated cation channel activity Source: UniProtKB
  • ligand-gated calcium channel activity Source: BHF-UCL
  • protein homodimerization activity Source: BHF-UCL
  • purinergic nucleotide receptor activity Source: BHF-UCL
  • signaling receptor binding Source: BHF-UCL
  • zinc ion binding Source: BHF-UCL

GO - Biological processi

  • apoptotic signaling pathway Source: BHF-UCL
  • behavioral response to pain Source: ARUK-UCL
  • blood coagulation Source: Reactome
  • calcium ion transmembrane transport Source: BHF-UCL
  • calcium-mediated signaling Source: ARUK-UCL
  • cellular response to ATP Source: UniProtKB
  • cellular response to zinc ion Source: UniProtKB
  • endothelial cell activation Source: BHF-UCL
  • ion transmembrane transport Source: UniProtKB
  • membrane depolarization Source: BHF-UCL
  • negative regulation of cardiac muscle hypertrophy Source: BHF-UCL
  • neuronal action potential Source: Ensembl
  • positive regulation of blood vessel endothelial cell migration Source: CAFA
  • positive regulation of calcium ion transport Source: BHF-UCL
  • positive regulation of calcium ion transport into cytosol Source: BHF-UCL
  • positive regulation of calcium-mediated signaling Source: BHF-UCL
  • positive regulation of endothelial cell chemotaxis Source: CAFA
  • positive regulation of microglial cell migration Source: ARUK-UCL
  • positive regulation of nitric oxide biosynthetic process Source: BHF-UCL
  • positive regulation of prostaglandin secretion Source: BHF-UCL
  • positive regulation of protein kinase B signaling Source: ARUK-UCL
  • protein homooligomerization Source: Ensembl
  • purinergic nucleotide receptor signaling pathway Source: BHF-UCL
  • regulation of blood pressure Source: BHF-UCL
  • regulation of cardiac muscle contraction Source: BHF-UCL
  • regulation of chemotaxis Source: ARUK-UCL
  • regulation of sodium ion transport Source: BHF-UCL
  • relaxation of cardiac muscle Source: BHF-UCL
  • response to ATP Source: BHF-UCL
  • response to axon injury Source: ARUK-UCL
  • response to fluid shear stress Source: BHF-UCL
  • response to ischemia Source: ARUK-UCL
  • sensory perception of pain Source: ARUK-UCL
  • sensory perception of touch Source: ARUK-UCL
  • signal transduction Source: BHF-UCL
  • tissue homeostasis Source: BHF-UCL

Keywordsi

Molecular functionIon channel, Ligand-gated ion channel, Receptor
Biological processIon transport, Transport

Enzyme and pathway databases

ReactomeiR-HSA-139853 Elevation of cytosolic Ca2+ levels
R-HSA-418346 Platelet homeostasis

Protein family/group databases

TCDBi1.A.7.1.5 the atp-gated p2x receptor cation channel (p2x receptor) family

Names & Taxonomyi

Protein namesi
Recommended name:
P2X purinoceptor 4
Short name:
P2X4
Alternative name(s):
ATP receptor
Purinergic receptor
Gene namesi
Name:P2RX4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiHostDB:ENSG00000135124.14
HGNCiHGNC:8535 P2RX4
MIMi600846 gene
neXtProtiNX_Q99571

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 33CytoplasmicSequence analysisAdd BLAST33
Transmembranei34 – 54Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini55 – 338ExtracellularSequence analysisAdd BLAST284
Transmembranei339 – 359Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini360 – 388CytoplasmicSequence analysisAdd BLAST29

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi119I → V: Does not change ATP-induced inward current. Does not change affinity for ATP. 1 Publication1

Organism-specific databases

DisGeNETi5025
OpenTargetsiENSG00000135124
PharmGKBiPA32864

Chemistry databases

ChEMBLiCHEMBL2104
DrugBankiDB09119 Eslicarbazepine acetate
GuidetoPHARMACOLOGYi481

Polymorphism and mutation databases

BioMutaiP2RX4
DMDMi116242696

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001615531 – 388P2X purinoceptor 4Add BLAST388

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi75N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi110N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi116 ↔ 165By similarity
Disulfide bondi126 ↔ 149By similarity
Disulfide bondi132 ↔ 159By similarity
Glycosylationi153N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi184N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi199N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi208N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi217 ↔ 227By similarity
Disulfide bondi261 ↔ 270By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiQ99571
MaxQBiQ99571
PaxDbiQ99571
PeptideAtlasiQ99571
PRIDEiQ99571
ProteomicsDBi78331

PTM databases

iPTMnetiQ99571
PhosphoSitePlusiQ99571

Expressioni

Gene expression databases

BgeeiENSG00000135124
CleanExiHS_P2RX4
ExpressionAtlasiQ99571 baseline and differential
GenevisibleiQ99571 HS

Organism-specific databases

HPAiHPA039494

Interactioni

Subunit structurei

Functional P2XRs are organized as homomeric and heteromeric trimers.By similarity

Binary interactionsi

Show more details

GO - Molecular functioni

  • cadherin binding Source: BHF-UCL
  • protein homodimerization activity Source: BHF-UCL
  • signaling receptor binding Source: BHF-UCL

Protein-protein interaction databases

BioGridi111064, 34 interactors
IntActiQ99571, 8 interactors
STRINGi9606.ENSP00000336607

Chemistry databases

BindingDBiQ99571

Structurei

3D structure databases

ProteinModelPortaliQ99571
SMRiQ99571
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the P2X receptor family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IFJF Eukaryota
ENOG410XR0C LUCA
GeneTreeiENSGT00390000016028
HOGENOMiHOG000232042
HOVERGENiHBG053086
InParanoidiQ99571
KOiK05218
OMAiTMINVGS
OrthoDBiEOG091G0A2U
PhylomeDBiQ99571
TreeFamiTF328633

Family and domain databases

Gene3Di2.60.490.10, 1 hit
InterProiView protein in InterPro
IPR003047 P2X4_purnocptor
IPR027309 P2X_extracellular_dom_sf
IPR001429 P2X_purnocptor
PANTHERiPTHR10125 PTHR10125, 1 hit
PfamiView protein in Pfam
PF00864 P2X_receptor, 1 hit
PIRSFiPIRSF005713 P2X_purinoceptor, 1 hit
PRINTSiPR01311 P2X4RECEPTOR
PR01307 P2XRECEPTOR
TIGRFAMsiTIGR00863 P2X, 1 hit
PROSITEiView protein in PROSITE
PS01212 P2X_RECEPTOR, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q99571-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGCCAALAA FLFEYDTPRI VLIRSRKVGL MNRAVQLLIL AYVIGWVFVW
60 70 80 90 100
EKGYQETDSV VSSVTTKVKG VAVTNTSKLG FRIWDVADYV IPAQEENSLF
110 120 130 140 150
VMTNVILTMN QTQGLCPEIP DATTVCKSDA SCTAGSAGTH SNGVSTGRCV
160 170 180 190 200
AFNGSVKTCE VAAWCPVEDD THVPQPAFLK AAENFTLLVK NNIWYPKFNF
210 220 230 240 250
SKRNILPNIT TTYLKSCIYD AKTDPFCPIF RLGKIVENAG HSFQDMAVEG
260 270 280 290 300
GIMGIQVNWD CNLDRAASLC LPRYSFRRLD TRDVEHNVSP GYNFRFAKYY
310 320 330 340 350
RDLAGNEQRT LIKAYGIRFD IIVFGKAGKF DIIPTMINIG SGLALLGMAT
360 370 380
VLCDIIVLYC MKKRLYYREK KYKYVEDYEQ GLASELDQ
Length:388
Mass (Da):43,369
Last modified:October 17, 2006 - v2
Checksum:iBA3BE7F30114C2A5
GO
Isoform 2 (identifier: Q99571-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     45-45: G → GCYHPHLAEVEMESPRR

Note: No experimental confirmation available.
Show »
Length:404
Mass (Da):45,305
Checksum:i0A8CB40D3D899F21
GO
Isoform 3 (identifier: Q99571-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     149-175: Missing.

Show »
Length:361
Mass (Da):40,481
Checksum:i9D09FA497870DDB1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti121D → S in AAB66834 (Ref. 8) Curated1
Sequence conflicti148R → W in AAB66834 (Ref. 8) Curated1
Sequence conflicti179L → F in AAB66834 (Ref. 8) Curated1
Sequence conflicti238N → I in AAB66834 (Ref. 8) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_0798563G → C1 PublicationCorresponds to variant dbSNP:rs200492184Ensembl.1
Natural variantiVAR_0283076A → S Polymorphism; does not change ATP-induced inward current; does not change affinity for ATP. 4 PublicationsCorresponds to variant dbSNP:rs1044249Ensembl.1
Natural variantiVAR_079857135G → S Polymorphism; does not change protein expression; does not affect membrane subcellular location; increases ATP-induced inward current. 1 PublicationCorresponds to variant dbSNP:rs765866317Ensembl.1
Natural variantiVAR_014942242S → G Polymorphism; does not change ATP-induced inward current; does not change affinity for ATP. 3 PublicationsCorresponds to variant dbSNP:rs25644Ensembl.1
Natural variantiVAR_079858315Y → C Rare polymorphism; may influence susceptibility to multiple sclerosis in the presence of variants M-205 and S-361 in P2RX7; does not affect membrane subcellular location; reduces ATP-induced inward current; decreases affinity for ATP. 4 PublicationsCorresponds to variant dbSNP:rs28360472Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_05381245G → GCYHPHLAEVEMESPRR in isoform 2. Curated1
Alternative sequenceiVSP_053813149 – 175Missing in isoform 3. 1 PublicationAdd BLAST27

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y07684 mRNA Translation: CAA68948.1
U87270
, U85971, U85972, U85973, U85974, U85975 Genomic DNA Translation: AAD00556.1
U83993 mRNA Translation: AAD00553.1
AF191093 Genomic DNA Translation: AAF06661.1
BT019738 mRNA Translation: AAV38543.1
BT019739 mRNA Translation: AAV38544.1
AC069209 Genomic DNA No translation available.
BC033826 mRNA Translation: AAH33826.1
AF000234 mRNA Translation: AAB58405.1
AF012903 mRNA Translation: AAB66834.1
CCDSiCCDS58282.1 [Q99571-2]
CCDS9214.1 [Q99571-1]
RefSeqiNP_001243725.1, NM_001256796.1 [Q99571-2]
NP_001248326.1, NM_001261397.1 [Q99571-3]
NP_002551.2, NM_002560.2 [Q99571-1]
UniGeneiHs.321709

Genome annotation databases

EnsembliENST00000337233; ENSP00000336607; ENSG00000135124 [Q99571-1]
ENST00000359949; ENSP00000353032; ENSG00000135124 [Q99571-2]
ENST00000542067; ENSP00000438329; ENSG00000135124 [Q99571-3]
GeneIDi5025
KEGGihsa:5025
UCSCiuc001tzr.4 human [Q99571-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiP2RX4_HUMAN
AccessioniPrimary (citable) accession number: Q99571
Secondary accession number(s): E7EPF7
, F6RU17, O00450, O14722, Q5U089, Q5U090, Q8N4N1, Q9UBG9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: October 17, 2006
Last modified: July 18, 2018
This is version 170 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health