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Entry version 168 (12 Aug 2020)
Sequence version 2 (01 Feb 2005)
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Protein

M-phase phosphoprotein 8

Gene

MPHOSPH8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Heterochromatin component that specifically recognizes and binds methylated 'Lys-9' of histone H3 (H3K9me) and promotes recruitment of proteins that mediate epigenetic repression (PubMed:20871592, PubMed:26022416). Mediates recruitment of the HUSH complex to H3K9me3 sites: the HUSH complex is recruited to genomic loci rich in H3K9me3 and is required to maintain transcriptional silencing by promoting recruitment of SETDB1, a histone methyltransferase that mediates further deposition of H3K9me3, as well as MORC2 (PubMed:26022416, PubMed:28581500). Binds H3K9me and promotes DNA methylation by recruiting DNMT3A to target CpG sites; these can be situated within the coding region of the gene (PubMed:20871592). Mediates down-regulation of CDH1 expression (PubMed:20871592). Also represses L1 retrotransposons in collaboration with MORC2 and, probably, SETDB1, the silencing is dependent of repressive epigenetic modifications, such as H3K9me3 mark. Silencing events often occur within introns of transcriptionally active genes, and lead to the down-regulation of host gene expression (PubMed:29211708). The HUSH complex is also involved in the silencing of unintegrated retroviral DNA by being recruited by ZNF638: some part of the retroviral DNA formed immediately after infection remains unintegrated in the host genome and is transcriptionally repressed (PubMed:30487602).5 Publications

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionRepressor
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

Pathway Commons web resource for biological pathway data

More...
PathwayCommonsi
Q99549

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
M-phase phosphoprotein 8
Alternative name(s):
Two hybrid-associated protein 3 with RanBPM1 Publication
Short name:
Twa31 Publication
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:MPHOSPH8Imported
Synonyms:MPP8
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiHomo sapiens (Human)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri9606 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000005640 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 13

Organism-specific databases

Eukaryotic Pathogen Database Resources

More...
EuPathDBi
HostDB:ENSG00000196199.13

Human Gene Nomenclature Database

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HGNCi
HGNC:29810, MPHOSPH8

Online Mendelian Inheritance in Man (OMIM)

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MIMi
611626, gene

neXtProt; the human protein knowledge platform

More...
neXtProti
NX_Q99549

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Nucleus

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi80W → A: Abolishes interaction with histone H3K9me3 and prevents recruitment of the HUSH complex to heterochromatin. Impaired ability to mediate silencing of unintegrated retroviral DNA. 3 Publications1
Mutagenesisi149S → A in STA mutant; fails to dissociate from chromatin during early mitosis; when associated with A-164; A-334 and A-385. 1 Publication1
Mutagenesisi164S → A in STA mutant; fails to dissociate from chromatin during early mitosis; when associated with A-149; A-334 and A-385. 1 Publication1
Mutagenesisi334T → A in STA mutant; fails to dissociate from chromatin during early mitosis; when associated with A-149; A-164 and A-385. 1 Publication1
Mutagenesisi385T → A in STA mutant; fails to dissociate from chromatin during early mitosis; when associated with A-149; A-164; and A-334. 1 Publication1

Organism-specific databases

DisGeNET

More...
DisGeNETi
54737

Open Targets

More...
OpenTargetsi
ENSG00000196199

The Pharmacogenetics and Pharmacogenomics Knowledge Base

More...
PharmGKBi
PA162396090

Miscellaneous databases

Pharos NIH Druggable Genome Knowledgebase

More...
Pharosi
Q99549, Tbio

Chemistry databases

ChEMBL database of bioactive drug-like small molecules

More...
ChEMBLi
CHEMBL1741210

Polymorphism and mutation databases

BioMuta curated single-nucleotide variation and disease association database

More...
BioMutai
MPHOSPH8

Domain mapping of disease mutations (DMDM)

More...
DMDMi
93204602

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000802441 – 860M-phase phosphoprotein 8Add BLAST860

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei1N-acetylmethionineCombined sources1
Modified residuei51PhosphoserineCombined sources1
Modified residuei85PhosphoserineBy similarity1
Modified residuei136PhosphoserineCombined sources1
Modified residuei138PhosphoserineCombined sources1
Modified residuei144PhosphothreonineBy similarity1
Modified residuei149Phosphoserine; by CDK1Combined sources1 Publication1
Modified residuei164Phosphoserine; by CDK11 Publication1
Modified residuei188PhosphoserineCombined sources1
Modified residuei189PhosphoserineCombined sources1
Modified residuei192PhosphoserineCombined sources1
Modified residuei266PhosphoserineBy similarity1
Modified residuei272PhosphoserineCombined sources1
Modified residuei279PhosphoserineCombined sources1
Modified residuei319PhosphoserineCombined sources1
Modified residuei334Phosphothreonine; by CDK11 Publication1
Modified residuei385Phosphothreonine; by CDK11 Publication1
Modified residuei392PhosphoserineCombined sources1
Modified residuei400PhosphoserineCombined sources1
Modified residuei403PhosphoserineCombined sources1
Modified residuei454PhosphothreonineCombined sources1

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

Phosphorylated in M (mitotic) phase. Phosphorylation by CDK1 promotes dissociation from chromatin.2 Publications

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

Encyclopedia of Proteome Dynamics

More...
EPDi
Q99549

jPOST - Japan Proteome Standard Repository/Database

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jPOSTi
Q99549

MassIVE - Mass Spectrometry Interactive Virtual Environment

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MassIVEi
Q99549

MaxQB - The MaxQuant DataBase

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MaxQBi
Q99549

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
Q99549

PeptideAtlas

More...
PeptideAtlasi
Q99549

PRoteomics IDEntifications database

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PRIDEi
Q99549

ProteomicsDB: a multi-organism proteome resource

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ProteomicsDBi
78321 [Q99549-1]
78322 [Q99549-2]

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...
iPTMneti
Q99549

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

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PhosphoSitePlusi
Q99549

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Bgee dataBase for Gene Expression Evolution

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Bgeei
ENSG00000196199, Expressed in frontal cortex and 234 other tissues

ExpressionAtlas, Differential and Baseline Expression

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ExpressionAtlasi
Q99549, baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

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Genevisiblei
Q99549, HS

Organism-specific databases

Human Protein Atlas

More...
HPAi
ENSG00000196199, Low tissue specificity

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Homodimer (PubMed:21419134, PubMed:22022377, PubMed:22086334).

Interacts (via chromo domain) with histone H3K9me3 (PubMed:20871592). Has the highest affinity for H3K9me3, and lesser affinity for H3K9me2 and H3K9me1 (PubMed:20871592).

Component of the HUSH complex; at least composed of TASOR, PPHLN1 and MPHOSPH8 (PubMed:26022416).

Interacts with DNMT3, EHMT1 and SETDB1 (PubMed:20871592, PubMed:22086334).

Interacts with MORC2; the interaction associateS MORC2 with the HUSH complex which recruits MORC2 to heterochromatic loci (PubMed:28581500).

Interacts with ZNF638; leading to recruitment of the HUSH complex to unintegrated retroviral DNA (PubMed:30487602).

7 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection describes interesting single amino acid sites on the sequence that are not defined in any other subsection. This subsection can be displayed in different sections ('Function', 'PTM / Processing', 'Pathology and Biotech') according to its content.<p><a href='/help/site' target='_top'>More...</a></p>Sitei59Interaction with histone H3K9me31 Publication1

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection">Interaction</a>' section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

Hide details

GO - Molecular functioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

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BioGRIDi
120119, 69 interactors

CORUM comprehensive resource of mammalian protein complexes

More...
CORUMi
Q99549

Database of interacting proteins

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DIPi
DIP-56224N

Protein interaction database and analysis system

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IntActi
Q99549, 54 interactors

Molecular INTeraction database

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MINTi
Q99549

STRING: functional protein association networks

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STRINGi
9606.ENSP00000355388

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...
RNActi
Q99549, protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1860
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

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SMRi
Q99549

Database of comparative protein structure models

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ModBasei
Search...

Protein Data Bank in Europe - Knowledge Base

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PDBe-KBi
Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

More...
EvolutionaryTracei
Q99549

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini59 – 118ChromoPROSITE-ProRule annotationAdd BLAST60
<p>This subsection of the 'Family and Domains' section indicates the positions and types of repeated sequence motifs or repeated domains within the protein.<p><a href='/help/repeat' target='_top'>More...</a></p>Repeati600 – 629ANK 1Add BLAST30
Repeati633 – 662ANK 2Add BLAST30
Repeati666 – 695ANK 3Add BLAST30
Repeati699 – 728ANK 4Add BLAST30

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni80 – 87Histone H3K9me3 binding1 Publication8

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes the position of regions of compositional bias within the protein and the particular amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi151 – 256Lys-richAdd BLAST106

<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

The chromo domain mediates interaction with methylated 'Lys-9' of histone H3 (H3K9me), with the highest affinity for the trimethylated form (H3K9me3).2 Publications

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
KOG0504, Eukaryota
KOG1911, Eukaryota

Ensembl GeneTree

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GeneTreei
ENSGT00730000111087

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

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HOGENOMi
CLU_332588_0_0_1

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
Q99549

KEGG Orthology (KO)

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KOi
K21871

Identification of Orthologs from Complete Genome Data

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OMAi
KYEPKEK

Database of Orthologous Groups

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OrthoDBi
1628171at2759

Database for complete collections of gene phylogenies

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PhylomeDBi
Q99549

TreeFam database of animal gene trees

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TreeFami
TF106394

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

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Gene3Di
1.25.40.20, 1 hit

Intrinsically Disordered proteins with Extensive Annotations and Literature

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IDEALi
IID00392

Integrated resource of protein families, domains and functional sites

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InterProi
View protein in InterPro
IPR002110, Ankyrin_rpt
IPR020683, Ankyrin_rpt-contain_dom
IPR036770, Ankyrin_rpt-contain_sf
IPR016197, Chromo-like_dom_sf
IPR000953, Chromo/chromo_shadow_dom
IPR023780, Chromo_domain
IPR023779, Chromodomain_CS

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF12796, Ank_2, 2 hits
PF00385, Chromo, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM00248, ANK, 4 hits
SM00298, CHROMO, 1 hit

Superfamily database of structural and functional annotation

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SUPFAMi
SSF48403, SSF48403, 1 hit
SSF54160, SSF54160, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS50297, ANK_REP_REGION, 1 hit
PS50088, ANK_REPEAT, 3 hits
PS00598, CHROMO_1, 1 hit
PS50013, CHROMO_2, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (2+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry describes 2 <p>This subsection of the 'Sequence' section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform. This section is only present in reviewed entries, i.e. in UniProtKB/Swiss-Prot.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q99549-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the <div> <p><b>What is the canonical sequence?</b><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MEQVAEGARV TAVPVSAADS TEELAEVEEG VGVVGEDNDA AARGAEAFGD
60 70 80 90 100
SEEDGEDVFE VEKILDMKTE GGKVLYKVRW KGYTSDDDTW EPEIHLEDCK
110 120 130 140 150
EVLLEFRKKI AENKAKAVRK DIQRLSLNND IFEANSDSDQ QSETKEDTSP
160 170 180 190 200
KKKKKKLRQR EEKSPDDLKK KKAKAGKLKD KSKPDLESSL ESLVFDLRTK
210 220 230 240 250
KRISEAKEEL KESKKPKKDE VKETKELKKV KKGEIRDLKT KTREDPKENR
260 270 280 290 300
KTKKEKFVES QVESESSVLN DSPFPEDDSE GLHSDSREEK QNTKSARERA
310 320 330 340 350
GQDMGLEHGF EKPLDSAMSA EEDTDVRGRR KKKTPRKAED TRENRKLENK
360 370 380 390 400
NAFLEKKTVP KKQRNQDRSK SAAELEKLMP VSAQTPKGRR LSGEERGLWS
410 420 430 440 450
TDSAEEDKET KRNESKEKYQ KRHDSDKEEK GRKEPKGLKT LKEIRNAFDL
460 470 480 490 500
FKLTPEEKND VSENNRKREE IPLDFKTIDD HKTKENKQSL KERRNTRDET
510 520 530 540 550
DTWAYIAAEG DQEVLDSVCQ ADENSDGRQQ ILSLGMDLQL EWMKLEDFQK
560 570 580 590 600
HLDGKDENFA ATDAIPSNVL RDAVKNGDYI TVKVALNSNE EYNLDQEDSS
610 620 630 640 650
GMTLVMLAAA GGQDDLLRLL ITKGAKVNGR QKNGTTALIH AAEKNFLTTV
660 670 680 690 700
AILLEAGAFV NVQQSNGETA LMKACKRGNS DIVRLVIECG ADCNILSKHQ
710 720 730 740 750
NSALHFAKQS NNVLVYDLLK NHLETLSRVA EETIKDYFEA RLALLEPVFP
760 770 780 790 800
IACHRLCEGP DFSTDFNYKP PQNIPEGSGI LLFIFHANFL GKEVIARLCG
810 820 830 840 850
PCSVQAVVLN DKFQLPVFLD SHFVYSFSPV AGPNKLFIRL TEAPSAKVKL
860
LIGAYRVQLQ
Length:860
Mass (Da):97,182
Last modified:February 1, 2005 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iDD75D14C3DBF5E95
GO
Isoform 2 (identifier: Q99549-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     820-860: DSHFVYSFSP...LIGAYRVQLQ → TGSRSVVQAG...GLKMHATTSG

Show »
Length:867
Mass (Da):97,637
Checksum:i5638702150529EA3
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0A0MT47A0A0A0MT47_HUMAN
M-phase phosphoprotein 8
MPHOSPH8
311Annotation score:

Annotation score:2 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
H0Y6J1H0Y6J1_HUMAN
M-phase phosphoprotein 8
MPHOSPH8
150Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

<p>This subsection of the 'Sequence' section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

The sequence AAH46214 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence BAB71284 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence CAI46172 differs from that shown. Reason: Frameshift.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti526 – 530DGRQQ → GEFGI in CAA66912 (PubMed:8885239).Curated5
Sequence conflicti860Q → QPNRRDWAEFS in AAH46214 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_031523820 – 860DSHFV…RVQLQ → TGSRSVVQAGVQWRGLQLTG VLTSQAQAILPPQPPNYLGL KMHATTSG in isoform 2. 2 PublicationsAdd BLAST41

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

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EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

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DDBJi
Links Updated
AK056785 mRNA Translation: BAB71284.1 Different initiation.
AK300258 mRNA Translation: BAH13245.1
AL832864 mRNA Translation: CAI46172.1 Frameshift.
AL354808 Genomic DNA No translation available.
AL359457 Genomic DNA No translation available.
CH471075 Genomic DNA Translation: EAX08228.1
CH471075 Genomic DNA Translation: EAX08230.1
BC003542 mRNA Translation: AAH03542.2
BC046214 mRNA Translation: AAH46214.1 Different initiation.
X98259 mRNA Translation: CAA66912.1

The Consensus CDS (CCDS) project

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CCDSi
CCDS9287.1 [Q99549-1]

NCBI Reference Sequences

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RefSeqi
NP_059990.2, NM_017520.3 [Q99549-1]

Genome annotation databases

Ensembl eukaryotic genome annotation project

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Ensembli
ENST00000361479; ENSP00000355388; ENSG00000196199 [Q99549-1]

Database of genes from NCBI RefSeq genomes

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GeneIDi
54737

KEGG: Kyoto Encyclopedia of Genes and Genomes

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KEGGi
hsa:54737

UCSC genome browser

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UCSCi
uc001umh.4, human [Q99549-1]

Keywords - Coding sequence diversityi

Alternative splicing

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK056785 mRNA Translation: BAB71284.1 Different initiation.
AK300258 mRNA Translation: BAH13245.1
AL832864 mRNA Translation: CAI46172.1 Frameshift.
AL354808 Genomic DNA No translation available.
AL359457 Genomic DNA No translation available.
CH471075 Genomic DNA Translation: EAX08228.1
CH471075 Genomic DNA Translation: EAX08230.1
BC003542 mRNA Translation: AAH03542.2
BC046214 mRNA Translation: AAH46214.1 Different initiation.
X98259 mRNA Translation: CAA66912.1
CCDSiCCDS9287.1 [Q99549-1]
RefSeqiNP_059990.2, NM_017520.3 [Q99549-1]

3D structure databases

Select the link destinations:

Protein Data Bank Europe

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PDBei

Protein Data Bank RCSB

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RCSB PDBi

Protein Data Bank Japan

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PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3LWEX-ray2.05A/B55-116[»]
3QO2X-ray2.49A/B/C/D55-116[»]
3R93X-ray2.06A/B/C/D55-116[»]
3SVMX-ray2.31A55-116[»]
6V2SX-ray1.60A/B55-116[»]
SMRiQ99549
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGRIDi120119, 69 interactors
CORUMiQ99549
DIPiDIP-56224N
IntActiQ99549, 54 interactors
MINTiQ99549
STRINGi9606.ENSP00000355388

Chemistry databases

ChEMBLiCHEMBL1741210

PTM databases

iPTMnetiQ99549
PhosphoSitePlusiQ99549

Polymorphism and mutation databases

BioMutaiMPHOSPH8
DMDMi93204602

Proteomic databases

EPDiQ99549
jPOSTiQ99549
MassIVEiQ99549
MaxQBiQ99549
PaxDbiQ99549
PeptideAtlasiQ99549
PRIDEiQ99549
ProteomicsDBi78321 [Q99549-1]
78322 [Q99549-2]

Protocols and materials databases

ABCD curated depository of sequenced antibodies

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ABCDi
Q99549, 1 sequenced antibody

Antibodypedia a portal for validated antibodies

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Antibodypediai
22243, 78 antibodies

Genome annotation databases

EnsembliENST00000361479; ENSP00000355388; ENSG00000196199 [Q99549-1]
GeneIDi54737
KEGGihsa:54737
UCSCiuc001umh.4, human [Q99549-1]

Organism-specific databases

Comparative Toxicogenomics Database

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CTDi
54737
DisGeNETi54737
EuPathDBiHostDB:ENSG00000196199.13

GeneCards: human genes, protein and diseases

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GeneCardsi
MPHOSPH8
HGNCiHGNC:29810, MPHOSPH8
HPAiENSG00000196199, Low tissue specificity
MIMi611626, gene
neXtProtiNX_Q99549
OpenTargetsiENSG00000196199
PharmGKBiPA162396090

GenAtlas: human gene database

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GenAtlasi
Search...

Phylogenomic databases

eggNOGiKOG0504, Eukaryota
KOG1911, Eukaryota
GeneTreeiENSGT00730000111087
HOGENOMiCLU_332588_0_0_1
InParanoidiQ99549
KOiK21871
OMAiKYEPKEK
OrthoDBi1628171at2759
PhylomeDBiQ99549
TreeFamiTF106394

Enzyme and pathway databases

PathwayCommonsiQ99549

Miscellaneous databases

BioGRID ORCS database of CRISPR phenotype screens

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BioGRID-ORCSi
54737, 96 hits in 875 CRISPR screens

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

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ChiTaRSi
MPHOSPH8, human
EvolutionaryTraceiQ99549

The Gene Wiki collection of pages on human genes and proteins

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GeneWikii
MPHOSPH8

Database of phenotypes from RNA interference screens in Drosophila and Homo sapiens

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GenomeRNAii
54737
PharosiQ99549, Tbio

Protein Ontology

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PROi
PR:Q99549
RNActiQ99549, protein

The Stanford Online Universal Resource for Clones and ESTs

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SOURCEi
Search...

Gene expression databases

BgeeiENSG00000196199, Expressed in frontal cortex and 234 other tissues
ExpressionAtlasiQ99549, baseline and differential
GenevisibleiQ99549, HS

Family and domain databases

Gene3Di1.25.40.20, 1 hit
IDEALiIID00392
InterProiView protein in InterPro
IPR002110, Ankyrin_rpt
IPR020683, Ankyrin_rpt-contain_dom
IPR036770, Ankyrin_rpt-contain_sf
IPR016197, Chromo-like_dom_sf
IPR000953, Chromo/chromo_shadow_dom
IPR023780, Chromo_domain
IPR023779, Chromodomain_CS
PfamiView protein in Pfam
PF12796, Ank_2, 2 hits
PF00385, Chromo, 1 hit
SMARTiView protein in SMART
SM00248, ANK, 4 hits
SM00298, CHROMO, 1 hit
SUPFAMiSSF48403, SSF48403, 1 hit
SSF54160, SSF54160, 1 hit
PROSITEiView protein in PROSITE
PS50297, ANK_REP_REGION, 1 hit
PS50088, ANK_REPEAT, 3 hits
PS00598, CHROMO_1, 1 hit
PS50013, CHROMO_2, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

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ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

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MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiMPP8_HUMAN
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q99549
Secondary accession number(s): B7Z6F9
, Q5JPE5, Q5JTQ0, Q86TK3, Q96MK4, Q9BTP1
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: February 1, 2005
Last modified: August 12, 2020
This is version 168 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Reference proteome

Documents

  1. Human chromosome 13
    Human chromosome 13: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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