UniProtKB - Q99490 (AGAP2_HUMAN)

BLAST

>sp|Q99490|AGAP2_HUMAN Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 OS=Homo sapiens OX=9606 GN=AGAP2 PE=1 SV=2
MSRGAGALQRRTTTYLISLTLVKLESVPPPPPSPSAAAVGAPGARGSEPRDPGSPRGAEE
PGKKRHERLFHRQDALWISTSSAGAGGAEPPALSPAPASPARPVSPAPGRRLSLWAAPPG
PPLSGGLSPDSKPGGAPSSSRRPLLSSPSWGGPEPEGRTGGGVPGSSSPHPGTGSRRLKV
APPPPAPKPCKTVTTSGAKAGGGKGAGSRLSWPESEGKPRVKGSKSSAGTGASVSAAATA
AAAGGGGSTASTSGGVGAGAGARGKLSPRKGKSKTLDNSDLHPGPPAGSPPPLTLPPTPS
PATAVTAASAQPPGPAPPITLEPPAPGLKRGREGGRASTRDRKMLKFISGIFTKSTGGPP
GSGPLPGPPSLSSGSGSRELLGAELRASPKAVINSQEWTLSRSIPELRLGVLGDARSGKS
SLIHRFLTGSYQVLEKTESEQYKKEMLVDGQTHLVLIREEAGAPDAKFSGWADAVIFVFS
LEDENSFQAVSRLHGQLSSLRGEGRGGLALALVGTQDRISASSPRVVGDARARALCADMK
RCSYYETCATYGLNVDRVFQEVAQKVVTLRKQQQLLAACKSLPSSPSHSAASTPVAGQAS
NGGHTSDYSSSLPSSPNVGHRELRAEAAAVAGLSTPGSLHRAAKRRTSLFANRRGSDSEK
RSLDSRGETTGSGRAIPIKQSFLLKRSGNSLNKEWKKKYVTLSSNGFLLYHPSINDYIHS
THGKEMDLLRTTVKVPGKRPPRAISAFGPSASINGLVKDMSTVQMGEGLEATTPMPSPSP
SPSSLQPPPDQTSKHLLKPDRNLARALSTDCTPSGDLSPLSREPPPSPMVKKQRRKKLTT
PSKTEGSAGQAEAKRKMWKLKSFGSLRNIYKAEENFEFLIVSSTGQTWHFEAASFEERDA
WVQAIESQILASLQCCESSKVKLRTDSQSEAVAIQAIRNAKGNSICVDCGAPNPTWASLN
LGALICIECSGIHRNLGTHLSRVRSLDLDDWPRELTLVLTAIGNDTANRVWESDTRGRAK
PSRDSSREERESWIRAKYEQLLFLAPLSTSEEPLGRQLWAAVQAQDVATVLLLLAHARHG
PLDTSVEDPQLRSPLHLAAELAHVVITQLLLWYGADVAARDAQGRTALFYARQAGSQLCA
DILLQHGCPGEGGSAATTPSAATTPSITATPSPRRRSSAASVGRADAPVALV

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History
Entry version 193 (12 Sep 2018)
Sequence version 2 (16 May 2006)
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Protein

Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2

Gene

AGAP2

Organism

Homo sapiens (Human)

Status

Reviewed-Annotation score: -Experimental evidence at protein levelⁱ

Functionⁱ

GTPase-activating protein (GAP) for ARF1 and ARF5, which also shows strong GTPase activity. Isoform 1 participates in the prevention of neuronal apoptosis by enhancing PI3 kinase activity. It aids the coupling of metabotropic glutamate receptor 1 (GRM1) to cytoplasmic PI3 kinase by interacting with Homer scaffolding proteins, and also seems to mediate anti-apoptotic effects of NGF by activating nuclear PI3 kinase. Isoform 2 does not stimulate PI3 kinase but may protect cells from apoptosis by stimulating Akt. It also regulates the adapter protein 1 (AP-1)-dependent trafficking of proteins in the endosomal system. It seems to be oncogenic. It is overexpressed in cancer cells, prevents apoptosis and promotes cancer cell invasion.4 Publications

Activity regulationⁱ

GAP activity is stimulated by phosphatidylinositol 4,5-bisphosphate (PIP2) and, to a lesser extent, by phosphatidylinositol 3,4,5-trisphosphate (PIP3). Phosphatidic acid potentiates PIP2 stimulation.

Regions

Feature key	Position(s)	DescriptionActions	Length
Nucleotide bindingⁱ	413 – 420	GTPSequence analysis	8
Nucleotide bindingⁱ	457 – 461	GTPSequence analysis	5
Nucleotide bindingⁱ	515 – 518	GTPSequence analysis	4
Zinc fingerⁱ	946 – 969	C4-typePROSITE-ProRule annotationAdd BLAST	24

GO - Molecular functionⁱ

GTPase activator activity Source: UniProtKB-KW
GTPase activity
Source: ARUK-UCL
Traceable author statementⁱ
- 18374643
GTP binding Source: UniProtKB-KW
metal ion binding Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

negative regulation of neuron apoptotic process
Source: MGI
Inferred from genetic interactionⁱ
- 18374643
negative regulation of protein catabolic process
Source: MGI
Inferred from direct assayⁱ
- 18374643
positive regulation of 1-phosphatidylinositol-3-kinase activity
Source: ARUK-UCL
Traceable author statementⁱ
- 18374643
protein transport Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	GTPase activation
Biological process	Protein transport, Transport
Ligand	GTP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

Reactomeⁱ	R-HSA-373752 Netrin-1 signaling
SignaLinkⁱ	Q99490
SIGNORⁱ	Q99490

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 Short name: AGAP-2 Alternative name(s): Centaurin-gamma-1 Short name: Cnt-g1 GTP-binding and GTPase-activating protein 2 Short name: GGAP2 Phosphatidylinositol 3-kinase enhancer Short name: PIKE
Gene namesⁱ	Name:AGAP2 Synonyms:CENTG1, KIAA0167
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 12

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000135439.11
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:16921 AGAP2
MIMⁱ	605476 gene
neXtProtⁱ	NX_Q99490

Keywords - Cellular componentⁱ

Cytoplasm, Nucleus

Pathology & Biotechⁱ

Keywords - Diseaseⁱ

Tumor suppressor

Organism-specific databases

DisGeNET More...DisGeNETⁱ	116986
Open Targets More...OpenTargetsⁱ	ENSG00000135439
PharmGKBⁱ	PA26411

Polymorphism and mutation databases

BioMutaⁱ	AGAP2
DMDMⁱ	97535883

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000074217	1 – 1192	Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2Add BLAST		1192

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	113	PhosphoserineBy similarity	1
Modified residueⁱ	128	PhosphoserineBy similarity	1
Modified residueⁱ	149	PhosphoserineBy similarity	1
Modified residueⁱ	638	PhosphoserineCombined sources	1
Modified residueⁱ	750	PhosphoserineBy similarity	1
Modified residueⁱ	752	PhosphoserineBy similarity	1
Modified residueⁱ	808	PhosphoserineBy similarity	1
Modified residueⁱ	927	PhosphoserineBy similarity	1
Modified residueⁱ	985	PhosphoserineBy similarity	1
Modified residueⁱ	1178	PhosphoserineBy similarity	1
Isoform 2 (identifier: Q99490-2)
Modified residueⁱ	682	Phosphotyrosine1 Publication	1
Modified residueⁱ	774	Phosphotyrosine1 Publication	1

Post-translational modificationⁱ

Isoform PIKE-A is phosphorylated at Tyr-682 and Tyr-774 by FYN, preventing its apoptotic cleavage.1 Publication

Keywords - PTMⁱ

Phosphoprotein

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q99490
PaxDbⁱ	Q99490
PeptideAtlas More...PeptideAtlasⁱ	Q99490
PRIDEⁱ	Q99490
ProteomicsDBⁱ	78295 78296 [Q99490-2]

PTM databases

iPTMnetⁱ	Q99490
PhosphoSitePlusⁱ	Q99490

Expressionⁱ

Tissue specificityⁱ

Isoform 1 is brain-specific. Isoform 2 is ubiquitously expressed, with highest levels in brain and heart.5 Publications

Gene expression databases

Bgeeⁱ	ENSG00000135439 Expressed in 182 organ(s), highest expression level in right hemisphere of cerebellum
CleanExⁱ	HS_AGAP2
ExpressionAtlasⁱ	Q99490 baseline and differential
Genevisibleⁱ	Q99490 HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	HPA023474

HPAⁱ

HPA023474

Interactionⁱ

Subunit structureⁱ

Isoform 1 interacts with EPB41L1, PLCG1, NF2, HOMER1 and HOMER2 (By similarity). Isoform 2 interacts with activated AKT1 in the presence of guanine nucleotides, and with the AP-1 complex.By similarity3 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
INSR	P06213	2	EBI-2361824,EBI-475899
Prlr	Q08501	2	EBI-7737644,EBI-7737664	From Mus musculus.
STAT5A	P42229	3	EBI-7737644,EBI-749537
Stat5a	P42230	2	EBI-7737644,EBI-617434	From Mus musculus.

Protein-protein interaction databases

BioGridⁱ	125549, 17 interactors
IntActⁱ	Q99490, 8 interactors
Molecular INTeraction database More...MINTⁱ	Q99490
STRINGⁱ	9606.ENSP00000449241

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Beta strandⁱ	406 – 412	Combined sources	7
Helixⁱ	415 – 417	Combined sources	3
Helixⁱ	419 – 428	Combined sources	10
Beta strandⁱ	438 – 448	Combined sources	11
Beta strandⁱ	451 – 459	Combined sources	9
Beta strandⁱ	461 – 463	Combined sources	3
Helixⁱ	466 – 471	Combined sources	6
Beta strandⁱ	473 – 480	Combined sources	8
Helixⁱ	484 – 501	Combined sources	18
Beta strandⁱ	503 – 505	Combined sources	3
Beta strandⁱ	509 – 515	Combined sources	7
Beta strandⁱ	521 – 523	Combined sources	3
Helixⁱ	529 – 539	Combined sources	11
Beta strandⁱ	540 – 548	Combined sources	9
Turnⁱ	549 – 552	Combined sources	4
Helixⁱ	555 – 574	Combined sources	20
Beta strandⁱ	677 – 686	Combined sources	10
Beta strandⁱ	695 – 703	Combined sources	9
Turnⁱ	704 – 706	Combined sources	3
Beta strandⁱ	707 – 713	Combined sources	7
Helixⁱ	714 – 719	Combined sources	6
Beta strandⁱ	724 – 730	Combined sources	7
Beta strandⁱ	732 – 734	Combined sources	3
Beta strandⁱ	878 – 881	Combined sources	4
Beta strandⁱ	887 – 894	Combined sources	8
Helixⁱ	895 – 913	Combined sources	19

Show more details

3D structure databases

ProteinModelPortalⁱ	Q99490
SMRⁱ	Q99490
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ	Q99490

EvolutionaryTraceⁱ

Q99490

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Length
Domainⁱ	676 – 910	PHPROSITE-ProRule annotationAdd BLAST	235
Domainⁱ	931 – 1051	Arf-GAPPROSITE-ProRule annotationAdd BLAST	121
Repeatⁱ	1090 – 1119	ANK 1Add BLAST	30
Repeatⁱ	1123 – 1152	ANK 2Add BLAST	30

Region

Feature key	Position(s)	DescriptionActions	Length
Regionⁱ	1 – 23	Interaction with EPB41L1By similarityAdd BLAST	23
Regionⁱ	180 – 225	Interactions with HOMER1 and NF2By similarityAdd BLAST	46
Regionⁱ	267 – 390	Interaction with PLCG1By similarityAdd BLAST	124
Regionⁱ	405 – 572	G domainAdd BLAST	168

Domainⁱ

G domain binds GTP and has GTPase activity.

Arf-GAP domain interacts with G domain and may regulate its GTPase activity.

Although both PH domains of isoforms 1 and 2 bind phospholipids, they differently regulate subcellular location. PH domain of isoform 1 directs the protein to the nucleus, but PH domain of isoform 2 directs it to the cytosol. PH domain of isoform 2 is required for binding to AP-1.

Sequence similaritiesⁱ

Belongs to the centaurin gamma-like family.Curated

Zinc finger

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Zinc fingerⁱ	946 – 969	C4-typePROSITE-ProRule annotationAdd BLAST		24

Keywords - Domainⁱ

ANK repeat, Repeat, Zinc-finger

Phylogenomic databases

eggNOGⁱ	KOG0705 Eukaryota COG5347 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00760000118874
HOGENOMⁱ	HOG000007233
HOVERGENⁱ	HBG054045
InParanoidⁱ	Q99490
KEGG Orthology (KO) More...KOⁱ	K17848
PhylomeDBⁱ	Q99490

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00204 ANK, 1 hit
Gene3Dⁱ	1.25.40.20, 1 hit 2.30.29.30, 2 hits 3.30.40.160, 1 hit
InterProⁱ	View protein in InterPro IPR002110 Ankyrin_rpt IPR020683 Ankyrin_rpt-contain_dom IPR036770 Ankyrin_rpt-contain_sf IPR037278 ARFGAP/RecO IPR001164 ArfGAP_dom IPR038508 ArfGAP_dom_sf IPR027417 P-loop_NTPase IPR011993 PH-like_dom_sf IPR001849 PH_domain IPR001806 Small_GTPase
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF12796 Ank_2, 1 hit PF01412 ArfGap, 1 hit PF00071 Ras, 1 hit
PRINTSⁱ	PR00405 REVINTRACTNG
SMARTⁱ	View protein in SMART SM00105 ArfGap, 1 hit SM00233 PH, 1 hit
SUPFAMⁱ	SSF48403 SSF48403, 1 hit SSF52540 SSF52540, 1 hit SSF57863 SSF57863, 1 hit
PROSITEⁱ	View protein in PROSITE PS50297 ANK_REP_REGION, 1 hit PS50088 ANK_REPEAT, 1 hit PS50115 ARFGAP, 1 hit PS50003 PH_DOMAIN, 1 hit PS51419 RAB, 1 hit

Sequences (2+)ⁱ

Sequence statusⁱ: Complete.

This entry describes 2 isoformsⁱ produced by alternative splicing. Align Add to basket

This entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show all Align All

Isoform 1 (identifier: Q99490-1) [UniParc]FASTA Add to basket

Also known as: PIKE-L

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

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        10         20         30         40         50
MSRGAGALQR RTTTYLISLT LVKLESVPPP PPSPSAAAVG APGARGSEPR 
        60         70         80         90        100
DPGSPRGAEE PGKKRHERLF HRQDALWIST SSAGAGGAEP PALSPAPASP 
       110        120        130        140        150
ARPVSPAPGR RLSLWAAPPG PPLSGGLSPD SKPGGAPSSS RRPLLSSPSW 
       160        170        180        190        200
GGPEPEGRTG GGVPGSSSPH PGTGSRRLKV APPPPAPKPC KTVTTSGAKA 
       210        220        230        240        250
GGGKGAGSRL SWPESEGKPR VKGSKSSAGT GASVSAAATA AAAGGGGSTA 
       260        270        280        290        300
STSGGVGAGA GARGKLSPRK GKSKTLDNSD LHPGPPAGSP PPLTLPPTPS 
       310        320        330        340        350
PATAVTAASA QPPGPAPPIT LEPPAPGLKR GREGGRASTR DRKMLKFISG 
       360        370        380        390        400
IFTKSTGGPP GSGPLPGPPS LSSGSGSREL LGAELRASPK AVINSQEWTL 
       410        420        430        440        450
SRSIPELRLG VLGDARSGKS SLIHRFLTGS YQVLEKTESE QYKKEMLVDG 
       460        470        480        490        500
QTHLVLIREE AGAPDAKFSG WADAVIFVFS LEDENSFQAV SRLHGQLSSL 
       510        520        530        540        550
RGEGRGGLAL ALVGTQDRIS ASSPRVVGDA RARALCADMK RCSYYETCAT 
       560        570        580        590        600
YGLNVDRVFQ EVAQKVVTLR KQQQLLAACK SLPSSPSHSA ASTPVAGQAS 
       610        620        630        640        650
NGGHTSDYSS SLPSSPNVGH RELRAEAAAV AGLSTPGSLH RAAKRRTSLF 
       660        670        680        690        700
ANRRGSDSEK RSLDSRGETT GSGRAIPIKQ SFLLKRSGNS LNKEWKKKYV 
       710        720        730        740        750
TLSSNGFLLY HPSINDYIHS THGKEMDLLR TTVKVPGKRP PRAISAFGPS 
       760        770        780        790        800
ASINGLVKDM STVQMGEGLE ATTPMPSPSP SPSSLQPPPD QTSKHLLKPD 
       810        820        830        840        850
RNLARALSTD CTPSGDLSPL SREPPPSPMV KKQRRKKLTT PSKTEGSAGQ 
       860        870        880        890        900
AEAKRKMWKL KSFGSLRNIY KAEENFEFLI VSSTGQTWHF EAASFEERDA 
       910        920        930        940        950
WVQAIESQIL ASLQCCESSK VKLRTDSQSE AVAIQAIRNA KGNSICVDCG 
       960        970        980        990       1000
APNPTWASLN LGALICIECS GIHRNLGTHL SRVRSLDLDD WPRELTLVLT 
      1010       1020       1030       1040       1050
AIGNDTANRV WESDTRGRAK PSRDSSREER ESWIRAKYEQ LLFLAPLSTS 
      1060       1070       1080       1090       1100
EEPLGRQLWA AVQAQDVATV LLLLAHARHG PLDTSVEDPQ LRSPLHLAAE 
      1110       1120       1130       1140       1150
LAHVVITQLL LWYGADVAAR DAQGRTALFY ARQAGSQLCA DILLQHGCPG 
      1160       1170       1180       1190 
EGGSAATTPS AATTPSITAT PSPRRRSSAA SVGRADAPVA LV

Length:1,192

Mass (Da):124,674

Last modified:May 16, 2006 - v2

Checksum:ⁱ8DA53707C0127984

Isoform 2 (identifier: Q99490-2) [UniParc]FASTA Add to basket

Also known as: PIKE-A

The sequence of this isoform differs from the canonical sequence as follows:
     1-336: Missing.
     337-390: ASTRDRKMLK...LGAELRASPK → MHAQRQFVVA...IQASLDSIRE
     853-872: Missing.

« Hide

        10         20         30         40         50
MHAQRQFVVA AVRAEVRRHE VAKQALNRLR KLAERVDDPE LQDSIQASLD 
        60         70         80         90        100
SIREAVINSQ EWTLSRSIPE LRLGVLGDAR SGKSSLIHRF LTGSYQVLEK 
       110        120        130        140        150
TESEQYKKEM LVDGQTHLVL IREEAGAPDA KFSGWADAVI FVFSLEDENS 
       160        170        180        190        200
FQAVSRLHGQ LSSLRGEGRG GLALALVGTQ DRISASSPRV VGDARARALC 
       210        220        230        240        250
ADMKRCSYYE TCATYGLNVD RVFQEVAQKV VTLRKQQQLL AACKSLPSSP 
       260        270        280        290        300
SHSAASTPVA GQASNGGHTS DYSSSLPSSP NVGHRELRAE AAAVAGLSTP 
       310        320        330        340        350
GSLHRAAKRR TSLFANRRGS DSEKRSLDSR GETTGSGRAI PIKQSFLLKR 
       360        370        380        390        400
SGNSLNKEWK KKYVTLSSNG FLLYHPSIND YIHSTHGKEM DLLRTTVKVP 
       410        420        430        440        450
GKRPPRAISA FGPSASINGL VKDMSTVQMG EGLEATTPMP SPSPSPSSLQ 
       460        470        480        490        500
PPPDQTSKHL LKPDRNLARA LSTDCTPSGD LSPLSREPPP SPMVKKQRRK 
       510        520        530        540        550
KLTTPSKTEG SAGQAEEENF EFLIVSSTGQ TWHFEAASFE ERDAWVQAIE 
       560        570        580        590        600
SQILASLQCC ESSKVKLRTD SQSEAVAIQA IRNAKGNSIC VDCGAPNPTW 
       610        620        630        640        650
ASLNLGALIC IECSGIHRNL GTHLSRVRSL DLDDWPRELT LVLTAIGNDT 
       660        670        680        690        700
ANRVWESDTR GRAKPSRDSS REERESWIRA KYEQLLFLAP LSTSEEPLGR 
       710        720        730        740        750
QLWAAVQAQD VATVLLLLAH ARHGPLDTSV EDPQLRSPLH LAAELAHVVI 
       760        770        780        790        800
TQLLLWYGAD VAARDAQGRT ALFYARQAGS QLCADILLQH GCPGEGGSAA 
       810        820        830 
TTPSAATTPS ITATPSPRRR SSAASVGRAD APVALV

Show »

Length:836

Mass (Da):90,537

Checksum:ⁱB8E42631BC06D5DC

Computationally mapped potential isoform sequencesⁱ

There are 3 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

F8VVT9	F8VVT9_HUMAN	Arf-GAP with GTPase, ANK repeat and... Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2	AGAP2	1,192	Annotation score:
J3KNM6	J3KNM6_HUMAN	Arf-GAP with GTPase, ANK repeat and... Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2	AGAP2	1,036	Annotation score:
H0YHB1	H0YHB1_HUMAN	Arf-GAP with GTPase, ANK repeat and... Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2	AGAP2	169	Annotation score:

Sequence cautionⁱ

The sequence BAA11484 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	1137	Q → H in AAC39522 (PubMed:9192850).Curated		1
Sequence conflictⁱ	1147	G → A in AAC39522 (PubMed:9192850).Curated		1

Natural variant

Feature key	Position(s)	DescriptionActions	Length
Natural variantⁱVAR_036183	339	T → A in a breast cancer sample; somatic mutation. 1 Publication	1
Natural variantⁱVAR_026438	455	V → A in a glioblastoma cell line. 1 Publication	1
Natural variantⁱVAR_022046	507	G → S. Corresponds to variant dbSNP:rs2301553Ensembl.	1
Natural variantⁱVAR_026439	518	R → G in a sarcoma cell line. 1 Publication	1
Natural variantⁱVAR_026440	568	T → I in a neuroblastoma cell line. 1 Publication	1
Natural variantⁱVAR_026441	651	A → V in a glioblastoma cell line. 1 Publication	1
Natural variantⁱVAR_026442	767	E → V in a glioblastoma cell line. 1 Publication	1
Natural variantⁱVAR_036184	816	D → Y in a breast cancer sample; somatic mutation. 1 Publication	1
Natural variantⁱVAR_026443	939	N → D in a glioblastoma cell line. 1 Publication	1
Natural variantⁱVAR_026444	947	V → M in a sarcoma cell line. 1 Publication	1
Natural variantⁱVAR_026445	1022	S → P in a glioblastoma cell line. 1 Publication	1
Natural variantⁱVAR_055532	1124	G → V1 PublicationCorresponds to variant dbSNP:rs238521Ensembl.	1

Alternative sequence

Feature key	Position(s)	DescriptionActions	Length
Alternative sequenceⁱVSP_018531	1 – 336	Missing in isoform 2. 5 PublicationsAdd BLAST	336
Alternative sequenceⁱVSP_018532	337 – 390	ASTRD…RASPK → MHAQRQFVVAAVRAEVRRHE VAKQALNRLRKLAERVDDPE LQDSIQASLDSIRE in isoform 2. 5 PublicationsAdd BLAST	54
Alternative sequenceⁱVSP_018533	853 – 872	Missing in isoform 2. 5 PublicationsAdd BLAST	20

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U81031 Genomic DNA Translation: AAC39522.2 AF384128 mRNA Translation: AAO39848.1 AY128689 mRNA Translation: AAM97540.1 D79989 mRNA Translation: BAA11484.2 Different initiation. AF413077 mRNA Translation: AAL04171.1 AK292672 mRNA Translation: BAF85361.1 CH471054 Genomic DNA Translation: EAW97049.1 BC028020 mRNA Translation: AAH28020.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS44932.1 [Q99490-1] CCDS8951.1 [Q99490-2]
NCBI Reference Sequences More...RefSeqⁱ	NP_001116244.1, NM_001122772.2 NP_055585.1, NM_014770.3 [Q99490-2]
UniGeneⁱ	Hs.302435

Genome annotation databases

Ensemblⁱ	ENST00000257897; ENSP00000257897; ENSG00000135439 [Q99490-2]
GeneIDⁱ	116986
KEGGⁱ	hsa:116986
UCSC genome browser More...UCSCⁱ	uc001spr.4 human [Q99490-1]

Keywords - Coding sequence diversityⁱ

Alternative splicing, Polymorphism

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q99490	Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2		MOUSE		1186	UniRef90_Q99490
Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2		RAT		1186
Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2		HUMAN		1192
AGAP2 isoform 2		PANTR		1192
arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 isoform X1		LEPWE		1189
+16
Q99490-2	Centaurin, gamma 1, isoform CRA_b		HUMAN		836	UniRef90_A0A024RB55
arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 isoform X2		NEOSC		843
Uncharacterized protein		PAPAN		837
Uncharacterized protein		NOMLE		837
Uncharacterized protein		MACNE		837
+89

Protein	Similar proteins		Species	Score	Length	Source
Q99490	Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2		MOUSE		1186	UniRef50_Q99490
Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2		RAT		1186
AGAP2 isoform 2		PANTR		1192
Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2		HUMAN		1192
arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 isoform X1		LEPWE		1189
+83
Q99490-2	Centaurin, gamma 1, isoform CRA_b		HUMAN		836	UniRef50_A0A024RB55
arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 isoform X2		NEOSC		843
Uncharacterized protein		MACNE		837
Uncharacterized protein		PAPAN		837
Uncharacterized protein		NOMLE		837
+224

Cross-referencesⁱ

Web resourcesⁱ

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U81031 Genomic DNA Translation: AAC39522.2 AF384128 mRNA Translation: AAO39848.1 AY128689 mRNA Translation: AAM97540.1 D79989 mRNA Translation: BAA11484.2 Different initiation. AF413077 mRNA Translation: AAL04171.1 AK292672 mRNA Translation: BAF85361.1 CH471054 Genomic DNA Translation: EAW97049.1 BC028020 mRNA Translation: AAH28020.1
CCDSⁱ	CCDS44932.1 [Q99490-1] CCDS8951.1 [Q99490-2]
RefSeqⁱ	NP_001116244.1, NM_001122772.2 NP_055585.1, NM_014770.3 [Q99490-2]
UniGeneⁱ	Hs.302435

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	2BMJ	X-ray	2.10	A	402-577	[»]
	2IWR	X-ray	1.50	A	402-577	[»]
	2RLO	NMR	-	A	674-914	[»]
ProteinModelPortalⁱ	Q99490
SMRⁱ	Q99490
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	125549, 17 interactors
IntActⁱ	Q99490, 8 interactors
MINTⁱ	Q99490
STRINGⁱ	9606.ENSP00000449241

PTM databases

iPTMnetⁱ	Q99490
PhosphoSitePlusⁱ	Q99490

Polymorphism and mutation databases

BioMutaⁱ	AGAP2
DMDMⁱ	97535883

Proteomic databases

MaxQBⁱ	Q99490
PaxDbⁱ	Q99490
PeptideAtlasⁱ	Q99490
PRIDEⁱ	Q99490
ProteomicsDBⁱ	78295 78296 [Q99490-2]

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENST00000257897; ENSP00000257897; ENSG00000135439 [Q99490-2]
GeneIDⁱ	116986
KEGGⁱ	hsa:116986
UCSCⁱ	uc001spr.4 human [Q99490-1]

Organism-specific databases

CTDⁱ	116986
DisGeNETⁱ	116986
EuPathDBⁱ	HostDB:ENSG00000135439.11
GeneCardsⁱ	AGAP2
H-InvDBⁱ	HIX0201900
HGNCⁱ	HGNC:16921 AGAP2
HPAⁱ	HPA023474
MIMⁱ	605476 gene
neXtProtⁱ	NX_Q99490
OpenTargetsⁱ	ENSG00000135439
PharmGKBⁱ	PA26411
HUGEⁱ	Search...
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	KOG0705 Eukaryota COG5347 LUCA
GeneTreeⁱ	ENSGT00760000118874
HOGENOMⁱ	HOG000007233
HOVERGENⁱ	HBG054045
InParanoidⁱ	Q99490
KOⁱ	K17848
PhylomeDBⁱ	Q99490

Enzyme and pathway databases

Reactomeⁱ	R-HSA-373752 Netrin-1 signaling
SignaLinkⁱ	Q99490
SIGNORⁱ	Q99490

Miscellaneous databases

ChiTaRSⁱ	AGAP2 human
EvolutionaryTraceⁱ	Q99490
GeneWikiⁱ	CENTG1
GenomeRNAiⁱ	116986
Protein Ontology More...PROⁱ	PR:Q99490
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000135439 Expressed in 182 organ(s), highest expression level in right hemisphere of cerebellum
CleanExⁱ	HS_AGAP2
ExpressionAtlasⁱ	Q99490 baseline and differential
Genevisibleⁱ	Q99490 HS

Family and domain databases

CDDⁱ	cd00204 ANK, 1 hit
Gene3Dⁱ	1.25.40.20, 1 hit 2.30.29.30, 2 hits 3.30.40.160, 1 hit
InterProⁱ	View protein in InterPro IPR002110 Ankyrin_rpt IPR020683 Ankyrin_rpt-contain_dom IPR036770 Ankyrin_rpt-contain_sf IPR037278 ARFGAP/RecO IPR001164 ArfGAP_dom IPR038508 ArfGAP_dom_sf IPR027417 P-loop_NTPase IPR011993 PH-like_dom_sf IPR001849 PH_domain IPR001806 Small_GTPase
Pfamⁱ	View protein in Pfam PF12796 Ank_2, 1 hit PF01412 ArfGap, 1 hit PF00071 Ras, 1 hit
PRINTSⁱ	PR00405 REVINTRACTNG
SMARTⁱ	View protein in SMART SM00105 ArfGap, 1 hit SM00233 PH, 1 hit
SUPFAMⁱ	SSF48403 SSF48403, 1 hit SSF52540 SSF52540, 1 hit SSF57863 SSF57863, 1 hit
PROSITEⁱ	View protein in PROSITE PS50297 ANK_REP_REGION, 1 hit PS50088 ANK_REPEAT, 1 hit PS50115 ARFGAP, 1 hit PS50003 PH_DOMAIN, 1 hit PS51419 RAB, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	AGAP2_HUMAN
Accessionⁱ	Q99490Primary (citable) accession number: Q99490 Secondary accession number(s): A8K9F7 , O00578, Q548E0, Q8IWU3
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
	Last sequence update:	May 16, 2006
	Last modified:	September 12, 2018
	This is version 193 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations
Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
PDB cross-references
Index of Protein Data Bank (PDB) cross-references
Human chromosome 12
Human chromosome 12: entries, gene names and cross-references to MIM

UniProtKB

UniParc

Help

UniRef

Proteomes

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Supporting data

UniProtKB - Q99490 (AGAP2_HUMAN)

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Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2

AGAP2

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Other locations

Other locations

Extracellular region or secreted

Mitochondrion

Nucleus

Other locations

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Keywords - Diseasei

Organism-specific databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Zinc finger

Keywords - Domaini

Phylogenomic databases

Family and domain databases

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

Experimental Info

Natural variant

Alternative sequence

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

<p>This subsection of the <a href="http://www.uniprot.org/manual/cross_references_section">Cross-references</a> section provides links to various web resources that are relevant for a specific protein.<p><a href='/help/web_resource' target='_top'>More...</a></p>Web resourcesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Polymorphism and mutation databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Your basket is currently empty. ⁱ

Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

Keywords - Diseaseⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Sequence cautionⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Web resourcesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ