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UniProtKB - Q99490 (AGAP2_HUMAN)

Entry version 212 (25 May 2022)
Sequence version 2 (16 May 2006)
Previous versions | rss
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Protein

Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2

Gene

AGAP2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

GTPase-activating protein (GAP) for ARF1 and ARF5, which also shows strong GTPase activity. Isoform 1 participates in the prevention of neuronal apoptosis by enhancing PI3 kinase activity. It aids the coupling of metabotropic glutamate receptor 1 (GRM1) to cytoplasmic PI3 kinase by interacting with Homer scaffolding proteins, and also seems to mediate anti-apoptotic effects of NGF by activating nuclear PI3 kinase. Isoform 2 does not stimulate PI3 kinase but may protect cells from apoptosis by stimulating Akt. It also regulates the adapter protein 1 (AP-1)-dependent trafficking of proteins in the endosomal system. It seems to be oncogenic. It is overexpressed in cancer cells, prevents apoptosis and promotes cancer cell invasion.

4 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/activity_regulation' target='_top'>More...</a></p>Activity regulationi

GAP activity is stimulated by phosphatidylinositol 4,5-bisphosphate (PIP2) and, to a lesser extent, by phosphatidylinositol 3,4,5-trisphosphate (PIP3). Phosphatidic acid potentiates PIP2 stimulation.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi413 – 420GTPSequence analysis8
Nucleotide bindingi457 – 461GTPSequence analysis5
Nucleotide bindingi515 – 518GTPSequence analysis4
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section specifies the position(s) and type(s) of zinc fingers within the protein.<p><a href='/help/zn_fing' target='_top'>More...</a></p>Zinc fingeri946 – 969C4-typePROSITE-ProRule annotationAdd BLAST24

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionGTPase activation
Biological processProtein transport, Transport
LigandGTP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

Pathway Commons web resource for biological pathway data

More...PathwayCommonsi		Q99490

Reactome - a knowledgebase of biological pathways and processes

More...Reactomei		R-HSA-373752, Netrin-1 signaling

SignaLink: a signaling pathway resource with multi-layered regulatory networks

More...SignaLinki		Q99490

SIGNOR Signaling Network Open Resource

More...SIGNORi		Q99490

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2
Short name:
AGAP-2
Alternative name(s):
Centaurin-gamma-1
Short name:
Cnt-g1
GTP-binding and GTPase-activating protein 2
Short name:
GGAP2
Phosphatidylinositol 3-kinase enhancer
Short name:
PIKE
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:AGAP2
Synonyms:CENTG1, KIAA0167
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiHomo sapiens (Human)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri9606 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000005640 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 12

Organism-specific databases

Human Gene Nomenclature Database

More...HGNCi		HGNC:16921, AGAP2

Online Mendelian Inheritance in Man (OMIM)

More...MIMi		605476, gene

neXtProt; the human protein knowledge platform

More...neXtProti		NX_Q99490

Eukaryotic Pathogen, Vector and Host Database Resources

More...VEuPathDBi		HostDB:ENSG00000135439

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

Cytoplasm, Nucleus

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Keywords - Diseasei

Tumor suppressor

Organism-specific databases

DisGeNET

More...DisGeNETi		116986

Open Targets

More...OpenTargetsi		ENSG00000135439

The Pharmacogenetics and Pharmacogenomics Knowledge Base

More...PharmGKBi		PA26411

Miscellaneous databases

Pharos NIH Druggable Genome Knowledgebase

More...Pharosi		Q99490, Tbio

Genetic variation databases

BioMuta curated single-nucleotide variation and disease association database

More...BioMutai		AGAP2

Domain mapping of disease mutations (DMDM)

More...DMDMi		97535883

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000742171 – 1192Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2Add BLAST1192

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei113PhosphoserineBy similarity1
Modified residuei128PhosphoserineBy similarity1
Modified residuei149PhosphoserineBy similarity1
Modified residuei638PhosphoserineCombined sources1
Modified residuei750PhosphoserineBy similarity1
Modified residuei752PhosphoserineBy similarity1
Modified residuei808PhosphoserineBy similarity1
Modified residuei927PhosphoserineBy similarity1
Modified residuei985PhosphoserineBy similarity1
Modified residuei1178PhosphoserineBy similarity1
Isoform 2 (identifier: Q99490-2)
Modified residuei682Phosphotyrosine1 Publication1
Modified residuei774Phosphotyrosine1 Publication1

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

Isoform PIKE-A is phosphorylated at Tyr-682 and Tyr-774 by FYN, preventing its apoptotic cleavage.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

Encyclopedia of Proteome Dynamics

More...EPDi		Q99490

jPOST - Japan Proteome Standard Repository/Database

More...jPOSTi		Q99490

MassIVE - Mass Spectrometry Interactive Virtual Environment

More...MassIVEi		Q99490

MaxQB - The MaxQuant DataBase

More...MaxQBi		Q99490

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		Q99490

PeptideAtlas

More...PeptideAtlasi		Q99490

PRoteomics IDEntifications database

More...PRIDEi		Q99490

ProteomicsDB: a multi-organism proteome resource

More...ProteomicsDBi		78295 [Q99490-1]
78296 [Q99490-2]

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...iPTMneti		Q99490

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

More...PhosphoSitePlusi		Q99490

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified 'at protein level'.<br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

Isoform 1 is brain-specific. Isoform 2 is ubiquitously expressed, with highest levels in brain and heart.5 Publications

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...Bgeei		ENSG00000135439, Expressed in right hemisphere of cerebellum and 196 other tissues

ExpressionAtlas, Differential and Baseline Expression

More...ExpressionAtlasi		Q99490, baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

More...Genevisiblei		Q99490, HS

Organism-specific databases

Human Protein Atlas

More...HPAi		ENSG00000135439, Tissue enriched (brain)

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Isoform 1 interacts with EPB41L1, PLCG1, NF2, HOMER1 and HOMER2 (By similarity).

Isoform 2 interacts with activated AKT1 in the presence of guanine nucleotides, and with the AP-1 complex.

By similarity3 Publications

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection">Interaction</a>' section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

Hide details

GO - Molecular functioni

Complete GO annotation on QuickGO ...

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...BioGRIDi		125549, 22 interactors

Protein interaction database and analysis system

More...IntActi		Q99490, 9 interactors

Molecular INTeraction database

More...MINTi		Q99490

STRING: functional protein association networks

More...STRINGi		9606.ENSP00000449241

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...RNActi		Q99490, protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

11192
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

AlphaFold Protein Structure Database

More...AlphaFoldDBi		Q99490

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		Q99490

Database of comparative protein structure models

More...ModBasei		Search...

Protein Data Bank in Europe - Knowledge Base

More...PDBe-KBi		Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

More...EvolutionaryTracei		Q99490

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini676 – 910PHPROSITE-ProRule annotationAdd BLAST235
Domaini931 – 1051Arf-GAPPROSITE-ProRule annotationAdd BLAST121
<p>This subsection of the 'Family and Domains' section indicates the positions and types of repeated sequence motifs or repeated domains within the protein.<p><a href='/help/repeat' target='_top'>More...</a></p>Repeati1090 – 1119ANK 1Add BLAST30
Repeati1123 – 1152ANK 2Add BLAST30

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni1 – 23Interaction with EPB41L1By similarityAdd BLAST23
Regioni24 – 377DisorderedSequence analysisAdd BLAST354
Regioni180 – 225Interactions with HOMER1 and NF2By similarityAdd BLAST46
Regioni267 – 390Interaction with PLCG1By similarityAdd BLAST124
Regioni405 – 572G domainAdd BLAST168
Regioni582 – 619DisorderedSequence analysisAdd BLAST38
Regioni634 – 673DisorderedSequence analysisAdd BLAST40
Regioni769 – 854DisorderedSequence analysisAdd BLAST86
Regioni1153 – 1192DisorderedSequence analysisAdd BLAST40

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes the position of regions of compositional bias within the protein and the particular type of amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi52 – 73Basic and acidic residuesSequence analysisAdd BLAST22
Compositional biasi284 – 299Pro residuesSequence analysisAdd BLAST16
Compositional biasi582 – 617Polar residuesSequence analysisAdd BLAST36
Compositional biasi650 – 667Basic and acidic residuesSequence analysisAdd BLAST18
Compositional biasi1156 – 1177Polar residuesSequence analysisAdd BLAST22

<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

G domain binds GTP and has GTPase activity.
Arf-GAP domain interacts with G domain and may regulate its GTPase activity.
Although both PH domains of isoforms 1 and 2 bind phospholipids, they differently regulate subcellular location. PH domain of isoform 1 directs the protein to the nucleus, but PH domain of isoform 2 directs it to the cytosol. PH domain of isoform 2 is required for binding to AP-1.

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the centaurin gamma-like family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri946 – 969C4-typePROSITE-ProRule annotationAdd BLAST24

Keywords - Domaini

ANK repeat, Repeat, Zinc-finger

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		KOG0705, Eukaryota

Ensembl GeneTree

More...GeneTreei		ENSGT00940000158956

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		CLU_007326_1_0_1

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		Q99490

Database for complete collections of gene phylogenies

More...PhylomeDBi		Q99490

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...Gene3Di		1.10.220.150, 1 hit
1.25.40.20, 1 hit
2.30.29.30, 2 hits
3.40.50.300, 1 hit

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR002110, Ankyrin_rpt
IPR036770, Ankyrin_rpt-contain_sf
IPR037278, ARFGAP/RecO
IPR001164, ArfGAP_dom
IPR038508, ArfGAP_dom_sf
IPR027417, P-loop_NTPase
IPR011993, PH-like_dom_sf
IPR001849, PH_domain
IPR001806, Small_GTPase

Pfam protein domain database

More...Pfami		View protein in Pfam
PF12796, Ank_2, 1 hit
PF01412, ArfGap, 1 hit
PF00071, Ras, 1 hit

Protein Motif fingerprint database; a protein domain database

More...PRINTSi		PR00405, REVINTRACTNG

Simple Modular Architecture Research Tool; a protein domain database

More...SMARTi		View protein in SMART
SM00105, ArfGap, 1 hit
SM00233, PH, 1 hit

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF48403, SSF48403, 1 hit
SSF52540, SSF52540, 1 hit
SSF57863, SSF57863, 1 hit

PROSITE; a protein domain and family database

More...PROSITEi		View protein in PROSITE
PS50297, ANK_REP_REGION, 1 hit
PS50088, ANK_REPEAT, 1 hit
PS50115, ARFGAP, 1 hit
PS50003, PH_DOMAIN, 1 hit
PS51419, RAB, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (2+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry describes 2 <p>This subsection of the 'Sequence' section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q99490-1) [UniParc]FASTAAdd to basket
Also known as: PIKE-L

This isoform has been chosen as the <p><strong>What is the canonical sequence?</strong><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSRGAGALQR RTTTYLISLT LVKLESVPPP PPSPSAAAVG APGARGSEPR 
        60         70         80         90        100
DPGSPRGAEE PGKKRHERLF HRQDALWIST SSAGAGGAEP PALSPAPASP 
       110        120        130        140        150
ARPVSPAPGR RLSLWAAPPG PPLSGGLSPD SKPGGAPSSS RRPLLSSPSW 
       160        170        180        190        200
GGPEPEGRTG GGVPGSSSPH PGTGSRRLKV APPPPAPKPC KTVTTSGAKA 
       210        220        230        240        250
GGGKGAGSRL SWPESEGKPR VKGSKSSAGT GASVSAAATA AAAGGGGSTA 
       260        270        280        290        300
STSGGVGAGA GARGKLSPRK GKSKTLDNSD LHPGPPAGSP PPLTLPPTPS 
       310        320        330        340        350
PATAVTAASA QPPGPAPPIT LEPPAPGLKR GREGGRASTR DRKMLKFISG 
       360        370        380        390        400
IFTKSTGGPP GSGPLPGPPS LSSGSGSREL LGAELRASPK AVINSQEWTL 
       410        420        430        440        450
SRSIPELRLG VLGDARSGKS SLIHRFLTGS YQVLEKTESE QYKKEMLVDG 
       460        470        480        490        500
QTHLVLIREE AGAPDAKFSG WADAVIFVFS LEDENSFQAV SRLHGQLSSL 
       510        520        530        540        550
RGEGRGGLAL ALVGTQDRIS ASSPRVVGDA RARALCADMK RCSYYETCAT 
       560        570        580        590        600
YGLNVDRVFQ EVAQKVVTLR KQQQLLAACK SLPSSPSHSA ASTPVAGQAS 
       610        620        630        640        650
NGGHTSDYSS SLPSSPNVGH RELRAEAAAV AGLSTPGSLH RAAKRRTSLF 
       660        670        680        690        700
ANRRGSDSEK RSLDSRGETT GSGRAIPIKQ SFLLKRSGNS LNKEWKKKYV 
       710        720        730        740        750
TLSSNGFLLY HPSINDYIHS THGKEMDLLR TTVKVPGKRP PRAISAFGPS 
       760        770        780        790        800
ASINGLVKDM STVQMGEGLE ATTPMPSPSP SPSSLQPPPD QTSKHLLKPD 
       810        820        830        840        850
RNLARALSTD CTPSGDLSPL SREPPPSPMV KKQRRKKLTT PSKTEGSAGQ 
       860        870        880        890        900
AEAKRKMWKL KSFGSLRNIY KAEENFEFLI VSSTGQTWHF EAASFEERDA 
       910        920        930        940        950
WVQAIESQIL ASLQCCESSK VKLRTDSQSE AVAIQAIRNA KGNSICVDCG 
       960        970        980        990       1000
APNPTWASLN LGALICIECS GIHRNLGTHL SRVRSLDLDD WPRELTLVLT 
      1010       1020       1030       1040       1050
AIGNDTANRV WESDTRGRAK PSRDSSREER ESWIRAKYEQ LLFLAPLSTS 
      1060       1070       1080       1090       1100
EEPLGRQLWA AVQAQDVATV LLLLAHARHG PLDTSVEDPQ LRSPLHLAAE 
      1110       1120       1130       1140       1150
LAHVVITQLL LWYGADVAAR DAQGRTALFY ARQAGSQLCA DILLQHGCPG 
      1160       1170       1180       1190 
EGGSAATTPS AATTPSITAT PSPRRRSSAA SVGRADAPVA LV
Length:1,192
Mass (Da):124,674
Last modified:May 16, 2006 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i8DA53707C0127984
GO
Isoform 2 (identifier: Q99490-2) [UniParc]FASTAAdd to basket
Also known as: PIKE-A

The sequence of this isoform differs from the canonical sequence as follows:
     1-336: Missing.
     337-390: ASTRDRKMLK...LGAELRASPK → MHAQRQFVVA...IQASLDSIRE
     853-872: Missing.

Show »
Length:836
Mass (Da):90,537
Checksum:iB8E42631BC06D5DC
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F8VVT9F8VVT9_HUMAN
Arf-GAP with GTPase, ANK repeat and...
AGAP2
1,192Annotation score:

Annotation score:3 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
J3KNM6J3KNM6_HUMAN
Arf-GAP with GTPase, ANK repeat and...
AGAP2
1,036Annotation score:

Annotation score:2 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
H0YHB1H0YHB1_HUMAN
Arf-GAP with GTPase, ANK repeat and...
AGAP2
169Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

<p>This subsection of the 'Sequence' section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

The sequence BAA11484 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti1137Q → H in AAC39522 (PubMed:9192850).Curated1
Sequence conflicti1147G → A in AAC39522 (PubMed:9192850).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes natural variant(s) of the protein sequence.<p><a href='/help/variant' target='_top'>More...</a></p>Natural variantiVAR_036183339T → A in a breast cancer sample; somatic mutation. 1 Publication1
Natural variantiVAR_026438455V → A in a glioblastoma cell line. 1 Publication1
Natural variantiVAR_022046507G → S. Corresponds to variant dbSNP:rs2301553Ensembl.1
Natural variantiVAR_026439518R → G in a sarcoma cell line. 1 Publication1
Natural variantiVAR_026440568T → I in a neuroblastoma cell line. 1 Publication1
Natural variantiVAR_026441651A → V in a glioblastoma cell line. 1 Publication1
Natural variantiVAR_026442767E → V in a glioblastoma cell line. 1 Publication1
Natural variantiVAR_036184816D → Y in a breast cancer sample; somatic mutation. 1 Publication1
Natural variantiVAR_026443939N → D in a glioblastoma cell line. 1 Publication1
Natural variantiVAR_026444947V → M in a sarcoma cell line. 1 Publication1
Natural variantiVAR_0264451022S → P in a glioblastoma cell line. 1 Publication1
Natural variantiVAR_0555321124G → V1 PublicationCorresponds to variant dbSNP:rs238521Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_0185311 – 336Missing in isoform 2. 5 PublicationsAdd BLAST336
Alternative sequenceiVSP_018532337 – 390ASTRD…RASPK → MHAQRQFVVAAVRAEVRRHE VAKQALNRLRKLAERVDDPE LQDSIQASLDSIRE in isoform 2. 5 PublicationsAdd BLAST54
Alternative sequenceiVSP_018533853 – 872Missing in isoform 2. 5 PublicationsAdd BLAST20

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
U81031 Genomic DNA Translation: AAC39522.2
AF384128 mRNA Translation: AAO39848.1
AY128689 mRNA Translation: AAM97540.1
D79989 mRNA Translation: BAA11484.2 Different initiation.
AF413077 mRNA Translation: AAL04171.1
AK292672 mRNA Translation: BAF85361.1
CH471054 Genomic DNA Translation: EAW97049.1
BC028020 mRNA Translation: AAH28020.1

The Consensus CDS (CCDS) project

More...CCDSi		CCDS44932.1 [Q99490-1]
CCDS8951.1 [Q99490-2]

NCBI Reference Sequences

More...RefSeqi		NP_001116244.1, NM_001122772.2
NP_055585.1, NM_014770.3 [Q99490-2]

Genome annotation databases

Ensembl eukaryotic genome annotation project

More...Ensembli		ENST00000257897.7; ENSP00000257897.3; ENSG00000135439.12 [Q99490-2]

Database of genes from NCBI RefSeq genomes

More...GeneIDi		116986

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		hsa:116986

UCSC genome browser

More...UCSCi		uc001spr.4, human [Q99490-1]

Keywords - Coding sequence diversityi

Alternative splicing

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

<p>This subsection of the <a href="http://www.uniprot.org/manual/cross%5Freferences%5Fsection">Cross-references</a> section provides links to various web resources that are relevant for a specific protein.<p><a href='/help/web_resource' target='_top'>More...</a></p>Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U81031 Genomic DNA Translation: AAC39522.2
AF384128 mRNA Translation: AAO39848.1
AY128689 mRNA Translation: AAM97540.1
D79989 mRNA Translation: BAA11484.2 Different initiation.
AF413077 mRNA Translation: AAL04171.1
AK292672 mRNA Translation: BAF85361.1
CH471054 Genomic DNA Translation: EAW97049.1
BC028020 mRNA Translation: AAH28020.1
CCDSiCCDS44932.1 [Q99490-1]
CCDS8951.1 [Q99490-2]
RefSeqiNP_001116244.1, NM_001122772.2
NP_055585.1, NM_014770.3 [Q99490-2]

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...PDBei

Protein Data Bank RCSB

More...RCSB PDBi

Protein Data Bank Japan

More...PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2BMJX-ray2.10A402-577[»]
2IWRX-ray1.50A402-577[»]
2RLONMR-A674-914[»]
AlphaFoldDBiQ99490
SMRiQ99490
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGRIDi125549, 22 interactors
IntActiQ99490, 9 interactors
MINTiQ99490
STRINGi9606.ENSP00000449241

PTM databases

iPTMnetiQ99490
PhosphoSitePlusiQ99490

Genetic variation databases

BioMutaiAGAP2
DMDMi97535883

Proteomic databases

EPDiQ99490
jPOSTiQ99490
MassIVEiQ99490
MaxQBiQ99490
PaxDbiQ99490
PeptideAtlasiQ99490
PRIDEiQ99490
ProteomicsDBi78295 [Q99490-1]
78296 [Q99490-2]

Protocols and materials databases

Antibodypedia a portal for validated antibodies

More...Antibodypediai		16354, 295 antibodies from 34 providers

The DNASU plasmid repository

More...DNASUi		116986

Genome annotation databases

EnsembliENST00000257897.7; ENSP00000257897.3; ENSG00000135439.12 [Q99490-2]
GeneIDi116986
KEGGihsa:116986
UCSCiuc001spr.4, human [Q99490-1]

Organism-specific databases

Comparative Toxicogenomics Database

More...CTDi		116986
DisGeNETi116986

GeneCards: human genes, protein and diseases

More...GeneCardsi		AGAP2
HGNCiHGNC:16921, AGAP2
HPAiENSG00000135439, Tissue enriched (brain)
MIMi605476, gene
neXtProtiNX_Q99490
OpenTargetsiENSG00000135439
PharmGKBiPA26411
VEuPathDBiHostDB:ENSG00000135439

Human Unidentified Gene-Encoded large proteins database

More...HUGEi		Search...

GenAtlas: human gene database

More...GenAtlasi		Search...

Phylogenomic databases

eggNOGiKOG0705, Eukaryota
GeneTreeiENSGT00940000158956
HOGENOMiCLU_007326_1_0_1
InParanoidiQ99490
PhylomeDBiQ99490

Enzyme and pathway databases

PathwayCommonsiQ99490
ReactomeiR-HSA-373752, Netrin-1 signaling
SignaLinkiQ99490
SIGNORiQ99490

Miscellaneous databases

BioGRID ORCS database of CRISPR phenotype screens

More...BioGRID-ORCSi		116986, 42 hits in 1071 CRISPR screens

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

More...ChiTaRSi		AGAP2, human
EvolutionaryTraceiQ99490

The Gene Wiki collection of pages on human genes and proteins

More...GeneWikii		CENTG1

Database of phenotypes from RNA interference screens in Drosophila and Homo sapiens

More...GenomeRNAii		116986
PharosiQ99490, Tbio

Protein Ontology

More...PROi		PR:Q99490
RNActiQ99490, protein

The Stanford Online Universal Resource for Clones and ESTs

More...SOURCEi		Search...

Gene expression databases

BgeeiENSG00000135439, Expressed in right hemisphere of cerebellum and 196 other tissues
ExpressionAtlasiQ99490, baseline and differential
GenevisibleiQ99490, HS

Family and domain databases

Gene3Di1.10.220.150, 1 hit
1.25.40.20, 1 hit
2.30.29.30, 2 hits
3.40.50.300, 1 hit
InterProiView protein in InterPro
IPR002110, Ankyrin_rpt
IPR036770, Ankyrin_rpt-contain_sf
IPR037278, ARFGAP/RecO
IPR001164, ArfGAP_dom
IPR038508, ArfGAP_dom_sf
IPR027417, P-loop_NTPase
IPR011993, PH-like_dom_sf
IPR001849, PH_domain
IPR001806, Small_GTPase
PfamiView protein in Pfam
PF12796, Ank_2, 1 hit
PF01412, ArfGap, 1 hit
PF00071, Ras, 1 hit
PRINTSiPR00405, REVINTRACTNG
SMARTiView protein in SMART
SM00105, ArfGap, 1 hit
SM00233, PH, 1 hit
SUPFAMiSSF48403, SSF48403, 1 hit
SSF52540, SSF52540, 1 hit
SSF57863, SSF57863, 1 hit
PROSITEiView protein in PROSITE
PS50297, ANK_REP_REGION, 1 hit
PS50088, ANK_REPEAT, 1 hit
PS50115, ARFGAP, 1 hit
PS50003, PH_DOMAIN, 1 hit
PS51419, RAB, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiAGAP2_HUMAN
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q99490
Secondary accession number(s): A8K9F7
, O00578, Q548E0, Q8IWU3
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: May 16, 2006
Last modified: May 25, 2022
This is version 212 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. Human entries with genetic variants
    List of human entries with genetic variants
  3. Human variants curated from literature reports
    Index of human variants curated from literature reports
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families
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