UniProtKB - Q99489 (OXDD_HUMAN)
Protein
D-aspartate oxidase
Gene
DDO
Organism
Homo sapiens (Human)
Status
Functioni
Selectively catalyzes the oxidative deamination of D-aspartate and its N-methylated derivative, N-methyl D-aspartate.
Catalytic activityi
- EC:1.4.3.1
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 166 | FAD; via amide nitrogen and carbonyl oxygenBy similarity | 1 | |
Binding sitei | 183 | FADBy similarity | 1 | |
Binding sitei | 223 | SubstrateBy similarity | 1 | |
Binding sitei | 278 | SubstrateBy similarity | 1 | |
Binding sitei | 312 | FADBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 6 – 20 | FADBy similarityAdd BLAST | 15 | |
Nucleotide bindingi | 36 – 37 | FADBy similarity | 2 | |
Nucleotide bindingi | 43 – 44 | FADBy similarity | 2 | |
Nucleotide bindingi | 48 – 50 | FADBy similarity | 3 | |
Nucleotide bindingi | 307 – 311 | FADBy similarity | 5 |
GO - Molecular functioni
- D-amino-acid oxidase activity Source: GO_Central
- D-aspartate oxidase activity Source: UniProtKB
- FAD binding Source: UniProtKB
GO - Biological processi
- aspartate catabolic process Source: UniProtKB
- aspartate metabolic process Source: GO_Central
- cellular nitrogen compound metabolic process Source: Reactome
- D-amino acid catabolic process Source: UniProtKB
- grooming behavior Source: Ensembl
- hormone metabolic process Source: Ensembl
- insemination Source: Ensembl
- protein targeting to peroxisome Source: Reactome
Keywordsi
Molecular function | Oxidoreductase |
Ligand | FAD, Flavoprotein |
Enzyme and pathway databases
PathwayCommonsi | Q99489 |
Reactomei | R-HSA-389661, Glyoxylate metabolism and glycine degradation R-HSA-9033241, Peroxisomal protein import |
SABIO-RKi | Q99489 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:DDO |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:2727, DDO |
MIMi | 124450, gene |
neXtProti | NX_Q99489 |
VEuPathDBi | HostDB:ENSG00000203797.9 |
Subcellular locationi
Peroxisome
Cytosol
- cytosol Source: HPA
Peroxisome
- peroxisomal matrix Source: Reactome
- peroxisome Source: UniProtKB
Other locations
- cytoplasm Source: GO_Central
Keywords - Cellular componenti
PeroxisomePathology & Biotechi
Organism-specific databases
DisGeNETi | 8528 |
OpenTargetsi | ENSG00000203797 |
PharmGKBi | PA27194 |
Miscellaneous databases
Pharosi | Q99489, Tchem |
Chemistry databases
ChEMBLi | CHEMBL5887 |
Genetic variation databases
BioMutai | DDO |
DMDMi | 2494037 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000162770 | 1 – 341 | D-aspartate oxidaseAdd BLAST | 341 |
Proteomic databases
MassIVEi | Q99489 |
MaxQBi | Q99489 |
PaxDbi | Q99489 |
PeptideAtlasi | Q99489 |
PRIDEi | Q99489 |
ProteomicsDBi | 78291 [Q99489-1] 78292 [Q99489-2] 78293 [Q99489-3] 78294 [Q99489-4] |
PTM databases
iPTMneti | Q99489 |
PhosphoSitePlusi | Q99489 |
Expressioni
Gene expression databases
Bgeei | ENSG00000203797, Expressed in heart and 178 other tissues |
ExpressionAtlasi | Q99489, baseline and differential |
Genevisiblei | Q99489, HS |
Organism-specific databases
HPAi | ENSG00000203797, Low tissue specificity |
Interactioni
Protein-protein interaction databases
BioGRIDi | 114098, 3 interactors |
IntActi | Q99489, 1 interactor |
STRINGi | 9606.ENSP00000357920 |
Chemistry databases
BindingDBi | Q99489 |
Miscellaneous databases
RNActi | Q99489, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q99489 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 339 – 341 | Microbody targeting signalSequence analysis | 3 |
Sequence similaritiesi
Belongs to the DAMOX/DASOX family.Curated
Phylogenomic databases
eggNOGi | KOG3923, Eukaryota |
GeneTreei | ENSGT00390000018635 |
HOGENOMi | CLU_034311_0_2_1 |
InParanoidi | Q99489 |
OMAi | MAIRERC |
OrthoDBi | 1363414at2759 |
PhylomeDBi | Q99489 |
TreeFami | TF313887 |
Family and domain databases
InterProi | View protein in InterPro IPR006181, D-amino_acid_oxidase_CS IPR023209, DAO IPR006076, FAD-dep_OxRdtase |
PANTHERi | PTHR11530, PTHR11530, 1 hit |
Pfami | View protein in Pfam PF01266, DAO, 1 hit |
PIRSFi | PIRSF000189, D-aa_oxidase, 1 hit |
PROSITEi | View protein in PROSITE PS00677, DAO, 1 hit |
s (4+)i Sequence
Sequence statusi: Complete.
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 4 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform DDO-1 (identifier: Q99489-1) [UniParc]FASTAAdd to basket
Also known as: A
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MDTARIAVVG AGVVGLSTAV CISKLVPRCS VTIISDKFTP DTTSDVAAGM
60 70 80 90 100
LIPHTYPDTP IHTQKQWFRE TFNHLFAIAN SAEAGDAGVH LVSGWQIFQS
110 120 130 140 150
TPTEEVPFWA DVVLGFRKMT EAELKKFPQY VFGQAFTTLK CECPAYLPWL
160 170 180 190 200
EKRIKGSGGW TLTRRIEDLW ELHPSFDIVV NCSGLGSRQL AGDSKIFPVR
210 220 230 240 250
GQVLQVQAPW VEHFIRDGSG LTYIYPGTSH VTLGGTRQKG DWNLSPDAEN
260 270 280 290 300
SREILSRCCA LEPSLHGACN IREKVGLRPY RPGVRLQTEL LARDGQRLPV
310 320 330 340
VHHYGHGSGG ISVHWGTALE AARLVSECVH ALRTPIPKSN L
Isoform DDO-2 (identifier: Q99489-2) [UniParc]FASTAAdd to basket
The sequence of this isoform differs from the canonical sequence as follows:
95-153: Missing.
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketF2Z2E0 | F2Z2E0_HUMAN | D-aspartate oxidase | DDO | 110 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length | |
---|---|---|---|---|---|
Sequence conflicti | 278 | R → S in BAF85718 (PubMed:14702039).Curated | 1 | ||
Isoform 4 (identifier: Q99489-4) | |||||
Sequence conflicti | 9 | T → N in DN990727 (Ref. 6) Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_036244 | 136 | F → L in a breast cancer sample; somatic mutation. 1 Publication | 1 | |
Natural variantiVAR_014939 | 189 | Q → E. Corresponds to variant dbSNP:rs17622Ensembl. | 1 | |
Natural variantiVAR_014940 | 230 | H → Y. Corresponds to variant dbSNP:rs17621Ensembl. | 1 | |
Natural variantiVAR_014941 | 255 | L → R. Corresponds to variant dbSNP:rs17623Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_037664 | 1 | M → MRPARHWETRFGARDFGGFQ DCFFRDRLM in isoform 3 and isoform 4. 3 Publications | 1 | |
Alternative sequenceiVSP_001269 | 95 – 153 | Missing in isoform DDO-2 and isoform 4. 2 PublicationsAdd BLAST | 59 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D89858 mRNA Translation: BAA14031.1 AK293029 mRNA Translation: BAF85718.1 AL050350 Genomic DNA No translation available. CH471051 Genomic DNA Translation: EAW48316.1 CH471051 Genomic DNA Translation: EAW48317.1 BC032786 mRNA Translation: AAH32786.3 DN990727 mRNA No translation available. |
CCDSi | CCDS5082.1 [Q99489-1] CCDS5083.1 [Q99489-2] |
PIRi | JC5438 JC5439 |
RefSeqi | NP_003640.2, NM_003649.2 NP_004023.2, NM_004032.2 [Q99489-2] |
Genome annotation databases
Ensembli | ENST00000368923; ENSP00000357919; ENSG00000203797 [Q99489-2] ENST00000368924; ENSP00000357920; ENSG00000203797 [Q99489-1] |
GeneIDi | 8528 |
KEGGi | hsa:8528 |
UCSCi | uc003puc.4, human [Q99489-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D89858 mRNA Translation: BAA14031.1 AK293029 mRNA Translation: BAF85718.1 AL050350 Genomic DNA No translation available. CH471051 Genomic DNA Translation: EAW48316.1 CH471051 Genomic DNA Translation: EAW48317.1 BC032786 mRNA Translation: AAH32786.3 DN990727 mRNA No translation available. |
CCDSi | CCDS5082.1 [Q99489-1] CCDS5083.1 [Q99489-2] |
PIRi | JC5438 JC5439 |
RefSeqi | NP_003640.2, NM_003649.2 NP_004023.2, NM_004032.2 [Q99489-2] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6RKF | X-ray | 3.22 | A/B/C/D/E/F | 1-341 | [»] | |
SMRi | Q99489 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 114098, 3 interactors |
IntActi | Q99489, 1 interactor |
STRINGi | 9606.ENSP00000357920 |
Chemistry databases
BindingDBi | Q99489 |
ChEMBLi | CHEMBL5887 |
PTM databases
iPTMneti | Q99489 |
PhosphoSitePlusi | Q99489 |
Genetic variation databases
BioMutai | DDO |
DMDMi | 2494037 |
Proteomic databases
MassIVEi | Q99489 |
MaxQBi | Q99489 |
PaxDbi | Q99489 |
PeptideAtlasi | Q99489 |
PRIDEi | Q99489 |
ProteomicsDBi | 78291 [Q99489-1] 78292 [Q99489-2] 78293 [Q99489-3] 78294 [Q99489-4] |
Protocols and materials databases
Antibodypediai | 32325, 216 antibodies |
Genome annotation databases
Ensembli | ENST00000368923; ENSP00000357919; ENSG00000203797 [Q99489-2] ENST00000368924; ENSP00000357920; ENSG00000203797 [Q99489-1] |
GeneIDi | 8528 |
KEGGi | hsa:8528 |
UCSCi | uc003puc.4, human [Q99489-1] |
Organism-specific databases
CTDi | 8528 |
DisGeNETi | 8528 |
GeneCardsi | DDO |
HGNCi | HGNC:2727, DDO |
HPAi | ENSG00000203797, Low tissue specificity |
MIMi | 124450, gene |
neXtProti | NX_Q99489 |
OpenTargetsi | ENSG00000203797 |
PharmGKBi | PA27194 |
VEuPathDBi | HostDB:ENSG00000203797.9 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3923, Eukaryota |
GeneTreei | ENSGT00390000018635 |
HOGENOMi | CLU_034311_0_2_1 |
InParanoidi | Q99489 |
OMAi | MAIRERC |
OrthoDBi | 1363414at2759 |
PhylomeDBi | Q99489 |
TreeFami | TF313887 |
Enzyme and pathway databases
PathwayCommonsi | Q99489 |
Reactomei | R-HSA-389661, Glyoxylate metabolism and glycine degradation R-HSA-9033241, Peroxisomal protein import |
SABIO-RKi | Q99489 |
Miscellaneous databases
BioGRID-ORCSi | 8528, 5 hits in 872 CRISPR screens |
ChiTaRSi | DDO, human |
GeneWikii | DDO_(gene) |
GenomeRNAii | 8528 |
Pharosi | Q99489, Tchem |
PROi | PR:Q99489 |
RNActi | Q99489, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000203797, Expressed in heart and 178 other tissues |
ExpressionAtlasi | Q99489, baseline and differential |
Genevisiblei | Q99489, HS |
Family and domain databases
InterProi | View protein in InterPro IPR006181, D-amino_acid_oxidase_CS IPR023209, DAO IPR006076, FAD-dep_OxRdtase |
PANTHERi | PTHR11530, PTHR11530, 1 hit |
Pfami | View protein in Pfam PF01266, DAO, 1 hit |
PIRSFi | PIRSF000189, D-aa_oxidase, 1 hit |
PROSITEi | View protein in PROSITE PS00677, DAO, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | OXDD_HUMAN | |
Accessioni | Q99489Primary (citable) accession number: Q99489 Secondary accession number(s): A8KAG4 Q8N552 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | May 1, 1997 | |
Last modified: | February 10, 2021 | |
This is version 177 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 6
Human chromosome 6: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families