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Protein

Tubulin-folding cofactor B

Gene

TBCB

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds to alpha-tubulin folding intermediates after their interaction with cytosolic chaperonin in the pathway leading from newly synthesized tubulin to properly folded heterodimer (PubMed:9265649). Involved in regulation of tubulin heterodimer dissociation. May function as a negative regulator of axonal growth (By similarity).By similarity1 Publication

GO - Biological processi

Keywordsi

Molecular functionChaperone, Developmental protein
Biological processDifferentiation, Neurogenesis

Enzyme and pathway databases

ReactomeiR-HSA-389977 Post-chaperonin tubulin folding pathway
SIGNORiQ99426

Names & Taxonomyi

Protein namesi
Recommended name:
Tubulin-folding cofactor B
Alternative name(s):
Cytoskeleton-associated protein 1
Cytoskeleton-associated protein CKAPI
Tubulin-specific chaperone B
Gene namesi
Name:TBCB
Synonyms:CG22, CKAP1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

EuPathDBiHostDB:ENSG00000105254.11
HGNCiHGNC:1989 TBCB
MIMi601303 gene
neXtProtiNX_Q99426

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi65S → A: Reduced phosphorylation by PAK1. Reduced microtubule polymerization and loss of phosphorylation by PAK1; when associated with A-128. 1 Publication1
Mutagenesisi128S → A: Reduced phosphorylation by PAK1. Reduced microtubule polymerization and loss of phosphorylation by PAK1; when associated with A-65. 1 Publication1

Organism-specific databases

DisGeNETi1155
OpenTargetsiENSG00000105254
PharmGKBiPA162405357

Polymorphism and mutation databases

BioMutaiTBCB
DMDMi3023518

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000835341 – 244Tubulin-folding cofactor BAdd BLAST244

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei65Phosphoserine; by PAK11 Publication1
Modified residuei98PhosphotyrosineCombined sources1
Modified residuei110PhosphoserineCombined sources1
Modified residuei128Phosphoserine; by PAK11 Publication1
Modified residuei219N6-acetyllysineCombined sources1

Post-translational modificationi

Phosphorylation by PAK1 is required for normal function.1 Publication
Ubiquitinated in the presence of GAN which targets it for degradation by the proteasome.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ99426
MaxQBiQ99426
PaxDbiQ99426
PeptideAtlasiQ99426
PRIDEiQ99426
ProteomicsDBi78261

2D gel databases

OGPiQ99426

PTM databases

iPTMnetiQ99426
PhosphoSitePlusiQ99426

Expressioni

Tissue specificityi

Found in most tissues.

Gene expression databases

BgeeiENSG00000105254 Expressed in 237 organ(s), highest expression level in C1 segment of cervical spinal cord
CleanExiHS_TBCB
ExpressionAtlasiQ99426 baseline and differential
GenevisibleiQ99426 HS

Organism-specific databases

HPAiHPA041428
HPA041722

Interactioni

Subunit structurei

Supercomplex made of cofactors A to E. Cofactors A and D function by capturing and stabilizing tubulin in a quasi-native conformation. Cofactor E binds to the cofactor D-tubulin complex; interaction with cofactor C then causes the release of tubulin polypeptides that are committed to the native state (PubMed:9265649). Cofactors B and E can form a heterodimer which binds to alpha-tubulin and enhances their ability to dissociate tubulin heterodimers (By similarity). Interacts with GAN (PubMed:16303566). Interacts with DCTN1 (PubMed:22777741).By similarity3 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi107575, 58 interactors
CORUMiQ99426
DIPiDIP-34409N
IntActiQ99426, 7 interactors
STRINGi9606.ENSP00000221855

Structurei

3D structure databases

ProteinModelPortaliQ99426
SMRiQ99426
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini183 – 225CAP-GlyPROSITE-ProRule annotationAdd BLAST43

Sequence similaritiesi

Belongs to the TBCB family.Curated

Phylogenomic databases

eggNOGiKOG3206 Eukaryota
ENOG410YKGB LUCA
GeneTreeiENSGT00760000119173
HOGENOMiHOG000209180
HOVERGENiHBG003239
InParanoidiQ99426
KOiK17262
OMAiYNEEEMQ
OrthoDBiEOG091G0M22
PhylomeDBiQ99426
TreeFamiTF313444

Family and domain databases

Gene3Di2.30.30.190, 1 hit
InterProiView protein in InterPro
IPR036859 CAP-Gly_dom_sf
IPR000938 CAP-Gly_domain
IPR029071 Ubiquitin-like_domsf
IPR000626 Ubiquitin_dom
PfamiView protein in Pfam
PF01302 CAP_GLY, 1 hit
PF14560 Ubiquitin_2, 1 hit
SMARTiView protein in SMART
SM01052 CAP_GLY, 1 hit
SUPFAMiSSF54236 SSF54236, 1 hit
SSF74924 SSF74924, 1 hit
PROSITEiView protein in PROSITE
PS00845 CAP_GLY_1, 1 hit
PS50245 CAP_GLY_2, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q99426-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MEVTGVSAPT VTVFISSSLN TFRSEKRYSR SLTIAEFKCK LELLVGSPAS
60 70 80 90 100
CMELELYGVD DKFYSKLDQE DALLGSYPVD DGCRIHVIDH SGARLGEYED
110 120 130 140 150
VSRVEKYTIS QEAYDQRQDT VRSFLKRSKL GRYNEEERAQ QEAEAAQRLA
160 170 180 190 200
EEKAQASSIP VGSRCEVRAA GQSPRRGTVM YVGLTDFKPG YWIGVRYDEP
210 220 230 240
LGKNDGSVNG KRYFECQAKY GAFVKPAVVT VGDFPEEDYG LDEI
Length:244
Mass (Da):27,326
Last modified:July 15, 1998 - v2
Checksum:iE984C7C74A105384
GO
Isoform 2 (identifier: Q99426-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-51: Missing.

Note: No experimental confirmation available.
Show »
Length:193
Mass (Da):21,801
Checksum:iF4C073C91A3AE400
GO

Computationally mapped potential isoform sequencesi

There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
K7EK42K7EK42_HUMAN
Tubulin-folding cofactor B
TBCB
191Annotation score:
K7EP07K7EP07_HUMAN
Tubulin-folding cofactor B
TBCB
169Annotation score:
K7ER04K7ER04_HUMAN
Tubulin-folding cofactor B
TBCB
60Annotation score:
K7EL99K7EL99_HUMAN
Tubulin-folding cofactor B
TBCB
133Annotation score:
K7EQH0K7EQH0_HUMAN
Tubulin-folding cofactor B
TBCB
45Annotation score:

Sequence cautioni

The sequence AAB51182 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti128S → R in AAB67716 (PubMed:9265649).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0555211 – 51Missing in isoform 2. 1 PublicationAdd BLAST51

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF013488 mRNA Translation: AAB67716.1
BT007424 mRNA Translation: AAP36092.1
CR541971 mRNA Translation: CAG46769.1
AD001527 Genomic DNA Translation: AAB51182.1 Sequence problems.
BC005969 mRNA Translation: AAH05969.1
BC052812 mRNA Translation: AAH52812.1
D49738 mRNA Translation: BAA08572.1
CCDSiCCDS12488.1 [Q99426-1]
CCDS74344.1 [Q99426-2]
RefSeqiNP_001272.2, NM_001281.2 [Q99426-1]
NP_001287900.1, NM_001300971.1 [Q99426-2]
UniGeneiHs.31053

Genome annotation databases

EnsembliENST00000221855; ENSP00000221855; ENSG00000105254 [Q99426-1]
ENST00000585746; ENSP00000467487; ENSG00000105254 [Q99426-2]
GeneIDi1155
KEGGihsa:1155
UCSCiuc002odg.2 human [Q99426-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF013488 mRNA Translation: AAB67716.1
BT007424 mRNA Translation: AAP36092.1
CR541971 mRNA Translation: CAG46769.1
AD001527 Genomic DNA Translation: AAB51182.1 Sequence problems.
BC005969 mRNA Translation: AAH05969.1
BC052812 mRNA Translation: AAH52812.1
D49738 mRNA Translation: BAA08572.1
CCDSiCCDS12488.1 [Q99426-1]
CCDS74344.1 [Q99426-2]
RefSeqiNP_001272.2, NM_001281.2 [Q99426-1]
NP_001287900.1, NM_001300971.1 [Q99426-2]
UniGeneiHs.31053

3D structure databases

ProteinModelPortaliQ99426
SMRiQ99426
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107575, 58 interactors
CORUMiQ99426
DIPiDIP-34409N
IntActiQ99426, 7 interactors
STRINGi9606.ENSP00000221855

PTM databases

iPTMnetiQ99426
PhosphoSitePlusiQ99426

Polymorphism and mutation databases

BioMutaiTBCB
DMDMi3023518

2D gel databases

OGPiQ99426

Proteomic databases

EPDiQ99426
MaxQBiQ99426
PaxDbiQ99426
PeptideAtlasiQ99426
PRIDEiQ99426
ProteomicsDBi78261

Protocols and materials databases

DNASUi1155
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000221855; ENSP00000221855; ENSG00000105254 [Q99426-1]
ENST00000585746; ENSP00000467487; ENSG00000105254 [Q99426-2]
GeneIDi1155
KEGGihsa:1155
UCSCiuc002odg.2 human [Q99426-1]

Organism-specific databases

CTDi1155
DisGeNETi1155
EuPathDBiHostDB:ENSG00000105254.11
GeneCardsiTBCB
HGNCiHGNC:1989 TBCB
HPAiHPA041428
HPA041722
MIMi601303 gene
neXtProtiNX_Q99426
OpenTargetsiENSG00000105254
PharmGKBiPA162405357
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3206 Eukaryota
ENOG410YKGB LUCA
GeneTreeiENSGT00760000119173
HOGENOMiHOG000209180
HOVERGENiHBG003239
InParanoidiQ99426
KOiK17262
OMAiYNEEEMQ
OrthoDBiEOG091G0M22
PhylomeDBiQ99426
TreeFamiTF313444

Enzyme and pathway databases

ReactomeiR-HSA-389977 Post-chaperonin tubulin folding pathway
SIGNORiQ99426

Miscellaneous databases

ChiTaRSiTBCB human
GeneWikiiTBCB
GenomeRNAii1155
PROiPR:Q99426
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000105254 Expressed in 237 organ(s), highest expression level in C1 segment of cervical spinal cord
CleanExiHS_TBCB
ExpressionAtlasiQ99426 baseline and differential
GenevisibleiQ99426 HS

Family and domain databases

Gene3Di2.30.30.190, 1 hit
InterProiView protein in InterPro
IPR036859 CAP-Gly_dom_sf
IPR000938 CAP-Gly_domain
IPR029071 Ubiquitin-like_domsf
IPR000626 Ubiquitin_dom
PfamiView protein in Pfam
PF01302 CAP_GLY, 1 hit
PF14560 Ubiquitin_2, 1 hit
SMARTiView protein in SMART
SM01052 CAP_GLY, 1 hit
SUPFAMiSSF54236 SSF54236, 1 hit
SSF74924 SSF74924, 1 hit
PROSITEiView protein in PROSITE
PS00845 CAP_GLY_1, 1 hit
PS50245 CAP_GLY_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTBCB_HUMAN
AccessioniPrimary (citable) accession number: Q99426
Secondary accession number(s): O00111
, O00674, O14728, Q6FGY5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 15, 1998
Last modified: November 7, 2018
This is version 168 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
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Main funding by: National Institutes of Health

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