TBCB

Functionⁱ

Binds to alpha-tubulin folding intermediates after their interaction with cytosolic chaperonin in the pathway leading from newly synthesized tubulin to properly folded heterodimer (PubMed:9265649). Involved in regulation of tubulin heterodimer dissociation. May function as a negative regulator of axonal growth (By similarity).By similarity1 Publication

GO - Biological processⁱ

cell differentiation Source: UniProtKB-KW
nervous system development Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Chaperone, Developmental protein
Biological process	Differentiation, Neurogenesis

Enzyme and pathway databases

Reactomeⁱ	R-HSA-389977 Post-chaperonin tubulin folding pathway
SIGNORⁱ	Q99426

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Tubulin-folding cofactor B Alternative name(s): Cytoskeleton-associated protein 1 Cytoskeleton-associated protein CKAPI Tubulin-specific chaperone B
Gene namesⁱ	Name:TBCB Synonyms:CG22, CKAP1
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 19

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000105254.11
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:1989 TBCB
MIMⁱ	601303 gene
neXtProtⁱ	NX_Q99426

Keywords - Cellular componentⁱ

Cytoplasm, Cytoskeleton, Microtubule

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Mutagenesisⁱ	65	S → A: Reduced phosphorylation by PAK1. Reduced microtubule polymerization and loss of phosphorylation by PAK1; when associated with A-128. 1 Publication		1
Mutagenesisⁱ	128	S → A: Reduced phosphorylation by PAK1. Reduced microtubule polymerization and loss of phosphorylation by PAK1; when associated with A-65. 1 Publication		1

Organism-specific databases

DisGeNET More...DisGeNETⁱ	1155
Open Targets More...OpenTargetsⁱ	ENSG00000105254
PharmGKBⁱ	PA162405357

Polymorphism and mutation databases

BioMutaⁱ	TBCB
DMDMⁱ	3023518

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000083534	1 – 244	Tubulin-folding cofactor BAdd BLAST		244

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	1	N-acetylmethionineCombined sources	1
Modified residueⁱ	65	Phosphoserine; by PAK11 Publication	1
Modified residueⁱ	98	PhosphotyrosineCombined sources	1
Modified residueⁱ	110	PhosphoserineCombined sources	1
Modified residueⁱ	128	Phosphoserine; by PAK11 Publication	1
Modified residueⁱ	219	N6-acetyllysineCombined sources	1

Post-translational modificationⁱ

Phosphorylation by PAK1 is required for normal function.1 Publication

Ubiquitinated in the presence of GAN which targets it for degradation by the proteasome.1 Publication

Keywords - PTMⁱ

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDⁱ	Q99426
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q99426
PaxDbⁱ	Q99426
PeptideAtlas More...PeptideAtlasⁱ	Q99426
PRIDEⁱ	Q99426
ProteomicsDBⁱ	78261

2D gel databases

USC-OGP 2-DE database More...OGPⁱ	Q99426

OGPⁱ

Q99426

PTM databases

iPTMnetⁱ	Q99426
PhosphoSitePlusⁱ	Q99426

Expressionⁱ

Tissue specificityⁱ

Found in most tissues.

Gene expression databases

Bgeeⁱ	ENSG00000105254 Expressed in 237 organ(s), highest expression level in C1 segment of cervical spinal cord
CleanExⁱ	HS_TBCB
ExpressionAtlasⁱ	Q99426 baseline and differential
Genevisibleⁱ	Q99426 HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	HPA041428 HPA041722

HPAⁱ

HPA041428
HPA041722

Interactionⁱ

Subunit structureⁱ

Supercomplex made of cofactors A to E. Cofactors A and D function by capturing and stabilizing tubulin in a quasi-native conformation. Cofactor E binds to the cofactor D-tubulin complex; interaction with cofactor C then causes the release of tubulin polypeptides that are committed to the native state (PubMed:9265649). Cofactors B and E can form a heterodimer which binds to alpha-tubulin and enhances their ability to dissociate tubulin heterodimers (By similarity). Interacts with GAN (PubMed:16303566). Interacts with DCTN1 (PubMed:22777741).By similarity3 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
GAN	Q9H2C0	3	EBI-764356,EBI-764342
USP7	Q93009	2	EBI-764356,EBI-302474

Protein-protein interaction databases

BioGridⁱ	107575, 58 interactors
CORUMⁱ	Q99426
Database of interacting proteins More...DIPⁱ	DIP-34409N
IntActⁱ	Q99426, 7 interactors
STRINGⁱ	9606.ENSP00000221855

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q99426
SMRⁱ	Q99426
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	183 – 225	CAP-GlyPROSITE-ProRule annotationAdd BLAST		43

Sequence similaritiesⁱ

Belongs to the TBCB family.Curated

Phylogenomic databases

eggNOGⁱ	KOG3206 Eukaryota ENOG410YKGB LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00760000119173
HOGENOMⁱ	HOG000209180
HOVERGENⁱ	HBG003239
InParanoidⁱ	Q99426
KEGG Orthology (KO) More...KOⁱ	K17262
OMAⁱ	YNEEEMQ
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0M22
PhylomeDBⁱ	Q99426
TreeFamⁱ	TF313444

Family and domain databases

Gene3Dⁱ	2.30.30.190, 1 hit
InterProⁱ	View protein in InterPro IPR036859 CAP-Gly_dom_sf IPR000938 CAP-Gly_domain IPR029071 Ubiquitin-like_domsf IPR000626 Ubiquitin_dom
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01302 CAP_GLY, 1 hit PF14560 Ubiquitin_2, 1 hit
SMARTⁱ	View protein in SMART SM01052 CAP_GLY, 1 hit
SUPFAMⁱ	SSF54236 SSF54236, 1 hit SSF74924 SSF74924, 1 hit
PROSITEⁱ	View protein in PROSITE PS00845 CAP_GLY_1, 1 hit PS50245 CAP_GLY_2, 1 hit

Sequences (2+)ⁱ

Sequence statusⁱ: Complete.

This entry describes 2 isoformsⁱ produced by alternative splicing. Align Add to basket

This entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.Show all Align All

Isoform 1 (identifier: Q99426-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEVTGVSAPT VTVFISSSLN TFRSEKRYSR SLTIAEFKCK LELLVGSPAS 
        60         70         80         90        100
CMELELYGVD DKFYSKLDQE DALLGSYPVD DGCRIHVIDH SGARLGEYED 
       110        120        130        140        150
VSRVEKYTIS QEAYDQRQDT VRSFLKRSKL GRYNEEERAQ QEAEAAQRLA 
       160        170        180        190        200
EEKAQASSIP VGSRCEVRAA GQSPRRGTVM YVGLTDFKPG YWIGVRYDEP 
       210        220        230        240 
LGKNDGSVNG KRYFECQAKY GAFVKPAVVT VGDFPEEDYG LDEI

Length:244

Mass (Da):27,326

Last modified:July 15, 1998 - v2

Checksum:ⁱE984C7C74A105384

GO

Isoform 2 (identifier: Q99426-2) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
1-51: Missing.

« Hide

        10         20         30         40         50
MELELYGVDD KFYSKLDQED ALLGSYPVDD GCRIHVIDHS GARLGEYEDV 
        60         70         80         90        100
SRVEKYTISQ EAYDQRQDTV RSFLKRSKLG RYNEEERAQQ EAEAAQRLAE 
       110        120        130        140        150
EKAQASSIPV GSRCEVRAAG QSPRRGTVMY VGLTDFKPGY WIGVRYDEPL 
       160        170        180        190 
GKNDGSVNGK RYFECQAKYG AFVKPAVVTV GDFPEEDYGL DEI

Note: No experimental confirmation available.

Show »

Length:193

Mass (Da):21,801

Checksum:ⁱF4C073C91A3AE400

GO

Computationally mapped potential isoform sequencesⁱ

There are 5 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

K7EK42	K7EK42_HUMAN	Tubulin-folding cofactor B Tubulin-folding cofactor B	TBCB	191	Annotation score:
K7EP07	K7EP07_HUMAN	Tubulin-folding cofactor B Tubulin-folding cofactor B	TBCB	169	Annotation score:
K7ER04	K7ER04_HUMAN	Tubulin-folding cofactor B Tubulin-folding cofactor B	TBCB	60	Annotation score:
K7EL99	K7EL99_HUMAN	Tubulin-folding cofactor B Tubulin-folding cofactor B	TBCB	133	Annotation score:
K7EQH0	K7EQH0_HUMAN	Tubulin-folding cofactor B Tubulin-folding cofactor B	TBCB	45	Annotation score:

Sequence cautionⁱ

The sequence AAB51182 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	128	S → R in AAB67716 (PubMed:9265649).Curated		1

Alternative sequence

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Alternative sequenceⁱVSP_055521	1 – 51	Missing in isoform 2. 1 PublicationAdd BLAST		51

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF013488 mRNA Translation: AAB67716.1 BT007424 mRNA Translation: AAP36092.1 CR541971 mRNA Translation: CAG46769.1 AD001527 Genomic DNA Translation: AAB51182.1 Sequence problems. BC005969 mRNA Translation: AAH05969.1 BC052812 mRNA Translation: AAH52812.1 D49738 mRNA Translation: BAA08572.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS12488.1 [Q99426-1] CCDS74344.1 [Q99426-2]
NCBI Reference Sequences More...RefSeqⁱ	NP_001272.2, NM_001281.2 [Q99426-1] NP_001287900.1, NM_001300971.1 [Q99426-2]
UniGeneⁱ	Hs.31053

Genome annotation databases

Ensemblⁱ	ENST00000221855; ENSP00000221855; ENSG00000105254 [Q99426-1] ENST00000585746; ENSP00000467487; ENSG00000105254 [Q99426-2]
GeneIDⁱ	1155
KEGGⁱ	hsa:1155
UCSC genome browser More...UCSCⁱ	uc002odg.2 human [Q99426-1]

Keywords - Coding sequence diversityⁱ

Alternative splicing

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q99426	TBCB isoform 2		PANTR		244	UniRef100_Q99426
Uncharacterized protein		PANPA		244
Tubulin folding cofactor B		GORGO		193
Isoform 2 of Tubulin-folding cofactor B		HUMAN		193
TBCB isoform 11		PANTR		193
+2

Protein	Similar proteins		Species	Score	Length	Source
Q99426	Tubulin-folding cofactor B	)	MOUSE		244	UniRef90_Q99426
TBCB isoform 2		PANTR		244
Uncharacterized protein		PANPA		244
Tubulin folding cofactor B		GORGO		193
Isoform 2 of Tubulin-folding cofactor B		HUMAN		193
+75

Protein	Similar proteins		Species	Score	Length	Source
Q99426	Tubulin-folding cofactor B	)	MOUSE		244	UniRef50_Q99426
Tubulin-folding cofactor B		BOVIN		244
TBCB isoform 2		PANTR		244
Uncharacterized protein		PANPA		244
Tubulin folding cofactor B		GORGO		193
+268

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF013488 mRNA Translation: AAB67716.1 BT007424 mRNA Translation: AAP36092.1 CR541971 mRNA Translation: CAG46769.1 AD001527 Genomic DNA Translation: AAB51182.1 Sequence problems. BC005969 mRNA Translation: AAH05969.1 BC052812 mRNA Translation: AAH52812.1 D49738 mRNA Translation: BAA08572.1
CCDSⁱ	CCDS12488.1 [Q99426-1] CCDS74344.1 [Q99426-2]
RefSeqⁱ	NP_001272.2, NM_001281.2 [Q99426-1] NP_001287900.1, NM_001300971.1 [Q99426-2]
UniGeneⁱ	Hs.31053

3D structure databases

ProteinModelPortalⁱ	Q99426
SMRⁱ	Q99426
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	107575, 58 interactors
CORUMⁱ	Q99426
DIPⁱ	DIP-34409N
IntActⁱ	Q99426, 7 interactors
STRINGⁱ	9606.ENSP00000221855

PTM databases

iPTMnetⁱ	Q99426
PhosphoSitePlusⁱ	Q99426

Polymorphism and mutation databases

BioMutaⁱ	TBCB
DMDMⁱ	3023518

2D gel databases

OGPⁱ	Q99426

OGPⁱ

Q99426

Proteomic databases

EPDⁱ	Q99426
MaxQBⁱ	Q99426
PaxDbⁱ	Q99426
PeptideAtlasⁱ	Q99426
PRIDEⁱ	Q99426
ProteomicsDBⁱ	78261

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	1155
Structural Biology Knowledgebase	Search...

Genome annotation databases

Ensemblⁱ	ENST00000221855; ENSP00000221855; ENSG00000105254 [Q99426-1] ENST00000585746; ENSP00000467487; ENSG00000105254 [Q99426-2]
GeneIDⁱ	1155
KEGGⁱ	hsa:1155
UCSCⁱ	uc002odg.2 human [Q99426-1]

Organism-specific databases

CTDⁱ	1155
DisGeNETⁱ	1155
EuPathDBⁱ	HostDB:ENSG00000105254.11
GeneCardsⁱ	TBCB
HGNCⁱ	HGNC:1989 TBCB
HPAⁱ	HPA041428 HPA041722
MIMⁱ	601303 gene
neXtProtⁱ	NX_Q99426
OpenTargetsⁱ	ENSG00000105254
PharmGKBⁱ	PA162405357
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	KOG3206 Eukaryota ENOG410YKGB LUCA
GeneTreeⁱ	ENSGT00760000119173
HOGENOMⁱ	HOG000209180
HOVERGENⁱ	HBG003239
InParanoidⁱ	Q99426
KOⁱ	K17262
OMAⁱ	YNEEEMQ
OrthoDBⁱ	EOG091G0M22
PhylomeDBⁱ	Q99426
TreeFamⁱ	TF313444

Enzyme and pathway databases

Reactomeⁱ	R-HSA-389977 Post-chaperonin tubulin folding pathway
SIGNORⁱ	Q99426

Miscellaneous databases

ChiTaRSⁱ	TBCB human
GeneWikiⁱ	TBCB
GenomeRNAiⁱ	1155
Protein Ontology More...PROⁱ	PR:Q99426
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000105254 Expressed in 237 organ(s), highest expression level in C1 segment of cervical spinal cord
CleanExⁱ	HS_TBCB
ExpressionAtlasⁱ	Q99426 baseline and differential
Genevisibleⁱ	Q99426 HS

Family and domain databases

Gene3Dⁱ	2.30.30.190, 1 hit
InterProⁱ	View protein in InterPro IPR036859 CAP-Gly_dom_sf IPR000938 CAP-Gly_domain IPR029071 Ubiquitin-like_domsf IPR000626 Ubiquitin_dom
Pfamⁱ	View protein in Pfam PF01302 CAP_GLY, 1 hit PF14560 Ubiquitin_2, 1 hit
SMARTⁱ	View protein in SMART SM01052 CAP_GLY, 1 hit
SUPFAMⁱ	SSF54236 SSF54236, 1 hit SSF74924 SSF74924, 1 hit
PROSITEⁱ	View protein in PROSITE PS00845 CAP_GLY_1, 1 hit PS50245 CAP_GLY_2, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	TBCB_HUMAN
Accessionⁱ	Q99426Primary (citable) accession number: Q99426 Secondary accession number(s): O00111 , O00674, O14728, Q6FGY5
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
	Last sequence update:	July 15, 1998
	Last modified:	November 7, 2018
	This is version 168 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
Human chromosome 19
Human chromosome 19: entries, gene names and cross-references to MIM

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UniProtKB - Q99426 (TBCB_HUMAN)

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Tubulin-folding cofactor B

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Cytoskeleton

Other locations

Cytoskeleton

Cytosol

Other locations

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Organism-specific databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

2D gel databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

Experimental Info

Alternative sequence

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Polymorphism and mutation databases

2D gel databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequence cautionⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ