UniProtKB - Q99426 (TBCB_HUMAN)
Tubulin-folding cofactor B
TBCB
Functioni
GO - Biological processi
- cell differentiation Source: UniProtKB-KW
- nervous system development Source: UniProtKB-KW
Keywordsi
Molecular function | Chaperone, Developmental protein |
Biological process | Differentiation, Neurogenesis |
Enzyme and pathway databases
PathwayCommonsi | Q99426 |
Reactomei | R-HSA-389977, Post-chaperonin tubulin folding pathway |
SIGNORi | Q99426 |
Names & Taxonomyi
Protein namesi | Recommended name: Tubulin-folding cofactor BAlternative name(s): Cytoskeleton-associated protein 1 Cytoskeleton-associated protein CKAPI Tubulin-specific chaperone B |
Gene namesi | Name:TBCB Synonyms:CG22, CKAP1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:1989, TBCB |
MIMi | 601303, gene |
neXtProti | NX_Q99426 |
VEuPathDBi | HostDB:ENSG00000105254.11 |
Subcellular locationi
Cytoskeleton
- cytoskeleton 1 Publication
Other locations
- Cytoplasm 2 Publications
Note: Colocalizes with microtubules. In differentiated neurons, located in the cytoplasm. In differentiating neurons, accumulates at the growth cone.1 Publication
Cytoskeleton
- microtubule Source: UniProtKB-KW
- microtubule cytoskeleton Source: ProtInc
Cytosol
- cytosol Source: HPA
Other locations
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, Cytoskeleton, MicrotubulePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 65 | S → A: Reduced phosphorylation by PAK1. Reduced microtubule polymerization and loss of phosphorylation by PAK1; when associated with A-128. 1 Publication | 1 | |
Mutagenesisi | 128 | S → A: Reduced phosphorylation by PAK1. Reduced microtubule polymerization and loss of phosphorylation by PAK1; when associated with A-65. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 1155 |
OpenTargetsi | ENSG00000105254 |
PharmGKBi | PA162405357 |
Miscellaneous databases
Pharosi | Q99426, Tbio |
Genetic variation databases
BioMutai | TBCB |
DMDMi | 3023518 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000083534 | 1 – 244 | Tubulin-folding cofactor BAdd BLAST | 244 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineCombined sources | 1 | |
Modified residuei | 65 | Phosphoserine; by PAK11 Publication | 1 | |
Modified residuei | 98 | PhosphotyrosineCombined sources | 1 | |
Modified residuei | 110 | PhosphoserineCombined sources | 1 | |
Modified residuei | 128 | Phosphoserine; by PAK11 Publication | 1 | |
Modified residuei | 219 | N6-acetyllysineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Phosphoprotein, Ubl conjugationProteomic databases
CPTACi | CPTAC-444 CPTAC-445 |
EPDi | Q99426 |
jPOSTi | Q99426 |
MassIVEi | Q99426 |
MaxQBi | Q99426 |
PaxDbi | Q99426 |
PeptideAtlasi | Q99426 |
PRIDEi | Q99426 |
ProteomicsDBi | 78261 [Q99426-1] |
2D gel databases
OGPi | Q99426 |
PTM databases
iPTMneti | Q99426 |
PhosphoSitePlusi | Q99426 |
SwissPalmi | Q99426 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000105254, Expressed in C1 segment of cervical spinal cord and 247 other tissues |
ExpressionAtlasi | Q99426, baseline and differential |
Genevisiblei | Q99426, HS |
Organism-specific databases
HPAi | ENSG00000105254, Tissue enhanced (blood) |
Interactioni
Subunit structurei
Supercomplex made of cofactors A to E. Cofactors A and D function by capturing and stabilizing tubulin in a quasi-native conformation. Cofactor E binds to the cofactor D-tubulin complex; interaction with cofactor C then causes the release of tubulin polypeptides that are committed to the native state (PubMed:9265649). Cofactors B and E can form a heterodimer which binds to alpha-tubulin and enhances their ability to dissociate tubulin heterodimers (By similarity).
Interacts with GAN (PubMed:16303566).
Interacts with DCTN1 (PubMed:22777741).
By similarity3 PublicationsBinary interactionsi
Hide detailsProtein-protein interaction databases
BioGRIDi | 107575, 67 interactors |
CORUMi | Q99426 |
DIPi | DIP-34409N |
IntActi | Q99426, 12 interactors |
STRINGi | 9606.ENSP00000221855 |
Miscellaneous databases
RNActi | Q99426, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 183 – 225 | CAP-GlyPROSITE-ProRule annotationAdd BLAST | 43 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG3206, Eukaryota |
GeneTreei | ENSGT00940000156119 |
HOGENOMi | CLU_067577_0_0_1 |
InParanoidi | Q99426 |
OMAi | VMYVGTT |
OrthoDBi | 1550378at2759 |
PhylomeDBi | Q99426 |
TreeFami | TF313444 |
Family and domain databases
Gene3Di | 2.30.30.190, 1 hit |
InterProi | View protein in InterPro IPR036859, CAP-Gly_dom_sf IPR000938, CAP-Gly_domain IPR000626, Ubiquitin-like_dom IPR029071, Ubiquitin-like_domsf |
Pfami | View protein in Pfam PF01302, CAP_GLY, 1 hit PF14560, Ubiquitin_2, 1 hit |
SMARTi | View protein in SMART SM01052, CAP_GLY, 1 hit |
SUPFAMi | SSF54236, SSF54236, 1 hit SSF74924, SSF74924, 1 hit |
PROSITEi | View protein in PROSITE PS00845, CAP_GLY_1, 1 hit PS50245, CAP_GLY_2, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MEVTGVSAPT VTVFISSSLN TFRSEKRYSR SLTIAEFKCK LELLVGSPAS
60 70 80 90 100
CMELELYGVD DKFYSKLDQE DALLGSYPVD DGCRIHVIDH SGARLGEYED
110 120 130 140 150
VSRVEKYTIS QEAYDQRQDT VRSFLKRSKL GRYNEEERAQ QEAEAAQRLA
160 170 180 190 200
EEKAQASSIP VGSRCEVRAA GQSPRRGTVM YVGLTDFKPG YWIGVRYDEP
210 220 230 240
LGKNDGSVNG KRYFECQAKY GAFVKPAVVT VGDFPEEDYG LDEI
Computationally mapped potential isoform sequencesi
There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketK7EK42 | K7EK42_HUMAN | Tubulin-folding cofactor B | TBCB | 191 | Annotation score: | ||
K7EP07 | K7EP07_HUMAN | Tubulin-folding cofactor B | TBCB | 169 | Annotation score: | ||
A0A494C0X0 | A0A494C0X0_HUMAN | Tubulin-folding cofactor B | TBCB | 282 | Annotation score: | ||
K7EL99 | K7EL99_HUMAN | Tubulin-folding cofactor B | TBCB | 133 | Annotation score: | ||
K7ER04 | K7ER04_HUMAN | Tubulin-folding cofactor B | TBCB | 60 | Annotation score: | ||
K7EQH0 | K7EQH0_HUMAN | Tubulin-folding cofactor B | TBCB | 45 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 128 | S → R in AAB67716 (PubMed:9265649).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_055521 | 1 – 51 | Missing in isoform 2. 1 PublicationAdd BLAST | 51 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF013488 mRNA Translation: AAB67716.1 BT007424 mRNA Translation: AAP36092.1 CR541971 mRNA Translation: CAG46769.1 AD001527 Genomic DNA Translation: AAB51182.1 Sequence problems. BC005969 mRNA Translation: AAH05969.1 BC052812 mRNA Translation: AAH52812.1 D49738 mRNA Translation: BAA08572.1 |
CCDSi | CCDS12488.1 [Q99426-1] CCDS74344.1 [Q99426-2] |
RefSeqi | NP_001272.2, NM_001281.2 [Q99426-1] NP_001287900.1, NM_001300971.1 [Q99426-2] |
Genome annotation databases
Ensembli | ENST00000221855; ENSP00000221855; ENSG00000105254 [Q99426-1] ENST00000585746; ENSP00000467487; ENSG00000105254 [Q99426-2] |
GeneIDi | 1155 |
KEGGi | hsa:1155 |
UCSCi | uc002odg.2, human [Q99426-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF013488 mRNA Translation: AAB67716.1 BT007424 mRNA Translation: AAP36092.1 CR541971 mRNA Translation: CAG46769.1 AD001527 Genomic DNA Translation: AAB51182.1 Sequence problems. BC005969 mRNA Translation: AAH05969.1 BC052812 mRNA Translation: AAH52812.1 D49738 mRNA Translation: BAA08572.1 |
CCDSi | CCDS12488.1 [Q99426-1] CCDS74344.1 [Q99426-2] |
RefSeqi | NP_001272.2, NM_001281.2 [Q99426-1] NP_001287900.1, NM_001300971.1 [Q99426-2] |
3D structure databases
SMRi | Q99426 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 107575, 67 interactors |
CORUMi | Q99426 |
DIPi | DIP-34409N |
IntActi | Q99426, 12 interactors |
STRINGi | 9606.ENSP00000221855 |
PTM databases
iPTMneti | Q99426 |
PhosphoSitePlusi | Q99426 |
SwissPalmi | Q99426 |
Genetic variation databases
BioMutai | TBCB |
DMDMi | 3023518 |
2D gel databases
OGPi | Q99426 |
Proteomic databases
CPTACi | CPTAC-444 CPTAC-445 |
EPDi | Q99426 |
jPOSTi | Q99426 |
MassIVEi | Q99426 |
MaxQBi | Q99426 |
PaxDbi | Q99426 |
PeptideAtlasi | Q99426 |
PRIDEi | Q99426 |
ProteomicsDBi | 78261 [Q99426-1] |
Protocols and materials databases
Antibodypediai | 29702, 279 antibodies |
DNASUi | 1155 |
Genome annotation databases
Ensembli | ENST00000221855; ENSP00000221855; ENSG00000105254 [Q99426-1] ENST00000585746; ENSP00000467487; ENSG00000105254 [Q99426-2] |
GeneIDi | 1155 |
KEGGi | hsa:1155 |
UCSCi | uc002odg.2, human [Q99426-1] |
Organism-specific databases
CTDi | 1155 |
DisGeNETi | 1155 |
GeneCardsi | TBCB |
HGNCi | HGNC:1989, TBCB |
HPAi | ENSG00000105254, Tissue enhanced (blood) |
MIMi | 601303, gene |
neXtProti | NX_Q99426 |
OpenTargetsi | ENSG00000105254 |
PharmGKBi | PA162405357 |
VEuPathDBi | HostDB:ENSG00000105254.11 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3206, Eukaryota |
GeneTreei | ENSGT00940000156119 |
HOGENOMi | CLU_067577_0_0_1 |
InParanoidi | Q99426 |
OMAi | VMYVGTT |
OrthoDBi | 1550378at2759 |
PhylomeDBi | Q99426 |
TreeFami | TF313444 |
Enzyme and pathway databases
PathwayCommonsi | Q99426 |
Reactomei | R-HSA-389977, Post-chaperonin tubulin folding pathway |
SIGNORi | Q99426 |
Miscellaneous databases
BioGRID-ORCSi | 1155, 604 hits in 879 CRISPR screens |
ChiTaRSi | TBCB, human |
GeneWikii | TBCB |
GenomeRNAii | 1155 |
Pharosi | Q99426, Tbio |
PROi | PR:Q99426 |
RNActi | Q99426, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000105254, Expressed in C1 segment of cervical spinal cord and 247 other tissues |
ExpressionAtlasi | Q99426, baseline and differential |
Genevisiblei | Q99426, HS |
Family and domain databases
Gene3Di | 2.30.30.190, 1 hit |
InterProi | View protein in InterPro IPR036859, CAP-Gly_dom_sf IPR000938, CAP-Gly_domain IPR000626, Ubiquitin-like_dom IPR029071, Ubiquitin-like_domsf |
Pfami | View protein in Pfam PF01302, CAP_GLY, 1 hit PF14560, Ubiquitin_2, 1 hit |
SMARTi | View protein in SMART SM01052, CAP_GLY, 1 hit |
SUPFAMi | SSF54236, SSF54236, 1 hit SSF74924, SSF74924, 1 hit |
PROSITEi | View protein in PROSITE PS00845, CAP_GLY_1, 1 hit PS50245, CAP_GLY_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TBCB_HUMAN | |
Accessioni | Q99426Primary (citable) accession number: Q99426 Secondary accession number(s): O00111 Q6FGY5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | July 15, 1998 | |
Last modified: | February 10, 2021 | |
This is version 185 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 19
Human chromosome 19: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families