UniProtKB - Q99385 (VCX1_YEAST)

BLAST

>sp|Q99385|VCX1_YEAST Vacuolar calcium ion transporter OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=VCX1 PE=1 SV=1
MDATTPLLTVANSHPARNPKHTAWRAAVYDLQYILKASPLNFLLVFVPLGLIWGHFQLSH
TLTFLFNFLAIIPLAAILANATEELADKAGNTIGGLLNATFGNAVELIVSIIALKKGQVR
IVQASMLGSLLSNLLLVLGLCFIFGGYNRVQQTFNQTAAQTMSSLLAIACASLLIPAAFR
ATLPHGKEDHFIDGKILELSRGTSIVILIVYVLFLYFQLGSHHALFEQQEEETDEVMSTI
SRNPHHSLSVKSSLVILLGTTVIISFCADFLVGTIDNVVESTGLSKTFIGLIVIPIVGNA
AEHVTSVLVAMKDKMDLALGVAIGSSLQVALFVTPFMVLVGWMIDVPMTLNFSTFETATL
FIAVFLSNYLILDGESNWLEGVMSLAMYILIAMAFFYYPDEKTLDSIGNSL

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History
Entry version 128 (07 Nov 2018)
Sequence version 1 (01 Nov 1996)
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Protein

Vacuolar calcium ion transporter

Gene

VCX1

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Status

Reviewed-Annotation score: -Experimental evidence at protein levelⁱ

Functionⁱ

Has a role in promoting intracellular calcium ion sequestration via the exchange of calcium ions for hydrogen ions across the vacuolar membrane. Involved also in manganese ion homeostasis via its uptake into the vacuole.2 Publications

Miscellaneous

Present with 7770 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functionⁱ

calcium:proton antiporter activity
Source: SGD
Inferred from direct assayⁱ
- 8628289
metal ion binding Source: UniProtKB-KW
potassium:proton antiporter activity
Source: SGD
Inferred from mutant phenotypeⁱ
- 20709757

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

calcium ion transmembrane transport
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
calcium ion transport
Source: SGD
Inferred from direct assayⁱ
- 8628289
cellular calcium ion homeostasis
Source: SGD
Inferred from mutant phenotypeⁱ
- 8628289
transmembrane transport
Source: SGD
Inferred from direct assayⁱ
- 8628289

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Biological process	Calcium transport, Ion transport, Transport
Ligand	Calcium, Manganese, Metal-binding

Enzyme and pathway databases

BioCycⁱ	YEAST:G3O-29527-MONOMER

BioCycⁱ

YEAST:G3O-29527-MONOMER

Protein family/group databases

TCDBⁱ	2.A.19.2.2 the ca(2+):cation antiporter (caca) family

TCDBⁱ

2.A.19.2.2 the ca(2+):cation antiporter (caca) family

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Vacuolar calcium ion transporter Alternative name(s): High copy number undoes manganese protein 1 Manganese resistance 1 protein Vacuolar Ca(2+)/H(+) exchanger
Gene namesⁱ	Name:VCX1 Synonyms:HUM1, MNR1 Ordered Locus Names:YDL128W
Organismⁱ	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifierⁱ	559292 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae
Proteomesⁱ	UP000002311 Componentⁱ: Chromosome IV

Organism-specific databases

Saccharomyces Genome Database More...SGDⁱ	S000002286 VCX1

SGDⁱ

S000002286 VCX1

Topology

Feature key	Position(s)	DescriptionActions	Length
Topological domainⁱ	1 – 30	CytoplasmicSequence analysisAdd BLAST	30
Transmembraneⁱ	31 – 53	HelicalSequence analysisAdd BLAST	23
Topological domainⁱ	54 – 59	VacuolarSequence analysis	6
Transmembraneⁱ	60 – 82	HelicalSequence analysisAdd BLAST	23
Topological domainⁱ	83 – 91	CytoplasmicSequence analysis	9
Transmembraneⁱ	92 – 114	HelicalSequence analysisAdd BLAST	23
Topological domainⁱ	115 – 126	VacuolarSequence analysisAdd BLAST	12
Transmembraneⁱ	127 – 146	HelicalSequence analysisAdd BLAST	20
Topological domainⁱ	147 – 160	CytoplasmicSequence analysisAdd BLAST	14
Transmembraneⁱ	161 – 183	HelicalSequence analysisAdd BLAST	23
Topological domainⁱ	184 – 203	VacuolarSequence analysisAdd BLAST	20
Transmembraneⁱ	204 – 226	HelicalSequence analysisAdd BLAST	23
Topological domainⁱ	227 – 252	CytoplasmicSequence analysisAdd BLAST	26
Transmembraneⁱ	253 – 275	HelicalSequence analysisAdd BLAST	23
Topological domainⁱ	276 – 287	VacuolarSequence analysisAdd BLAST	12
Transmembraneⁱ	288 – 310	HelicalSequence analysisAdd BLAST	23
Topological domainⁱ	311 – 319	CytoplasmicSequence analysis	9
Transmembraneⁱ	320 – 342	HelicalSequence analysisAdd BLAST	23
Topological domainⁱ	343 – 348	VacuolarSequence analysis	6
Transmembraneⁱ	349 – 371	HelicalSequence analysisAdd BLAST	23
Topological domainⁱ	372 – 380	CytoplasmicSequence analysis	9
Transmembraneⁱ	381 – 398	HelicalSequence analysisAdd BLAST	18
Topological domainⁱ	399 – 411	VacuolarSequence analysisAdd BLAST	13

Keywords - Cellular componentⁱ

Membrane, Vacuole

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000209504	1 – 411	Vacuolar calcium ion transporterAdd BLAST		411

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Modified residueⁱ	1	N-acetylmethionineCombined sources		1
Modified residueⁱ	13	PhosphoserineCombined sources		1

Keywords - PTMⁱ

Acetylation, Phosphoprotein

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q99385
PaxDbⁱ	Q99385
PRIDEⁱ	Q99385

PTM databases

iPTMnetⁱ	Q99385

iPTMnetⁱ

Q99385

Interactionⁱ

Protein-protein interaction databases

BioGridⁱ	31935, 39 interactors
Database of interacting proteins More...DIPⁱ	DIP-5039N
IntActⁱ	Q99385, 1 interactor
STRINGⁱ	4932.YDL128W

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Helixⁱ	25 – 36	Combined sources	12
Helixⁱ	41 – 45	Combined sources	5
Helixⁱ	46 – 56	Combined sources	11
Helixⁱ	60 – 87	Combined sources	28
Helixⁱ	91 – 100	Combined sources	10
Helixⁱ	101 – 103	Combined sources	3
Helixⁱ	104 – 114	Combined sources	11
Turnⁱ	115 – 117	Combined sources	3
Helixⁱ	119 – 134	Combined sources	16
Helixⁱ	136 – 146	Combined sources	11
Helixⁱ	158 – 181	Combined sources	24
Helixⁱ	193 – 219	Combined sources	27
Turnⁱ	220 – 222	Combined sources	3
Helixⁱ	223 – 241	Combined sources	19
Helixⁱ	250 – 273	Combined sources	24
Helixⁱ	275 – 281	Combined sources	7
Helixⁱ	286 – 292	Combined sources	7
Helixⁱ	294 – 311	Combined sources	18
Helixⁱ	315 – 331	Combined sources	17
Helixⁱ	333 – 343	Combined sources	11
Helixⁱ	354 – 373	Combined sources	20
Helixⁱ	378 – 396	Combined sources	19

Show more details

3D structure databases

ProteinModelPortalⁱ	Q99385
SMRⁱ	Q99385
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19) family. [View classification]Curated

Keywords - Domainⁱ

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMⁱ	HOG000083011
InParanoidⁱ	Q99385
KEGG Orthology (KO) More...KOⁱ	K07300
OMAⁱ	SNWLEGT
Database of Orthologous Groups More...OrthoDBⁱ	EOG092C2NQI

Family and domain databases

InterProⁱ	View protein in InterPro IPR004713 CaH_exchang IPR004798 CAX IPR004837 NaCa_Exmemb
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01699 Na_Ca_ex, 2 hits
TIGRFAMsⁱ	TIGR00846 caca2, 1 hit TIGR00378 cax, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Q99385-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MDATTPLLTV ANSHPARNPK HTAWRAAVYD LQYILKASPL NFLLVFVPLG 
        60         70         80         90        100
LIWGHFQLSH TLTFLFNFLA IIPLAAILAN ATEELADKAG NTIGGLLNAT 
       110        120        130        140        150
FGNAVELIVS IIALKKGQVR IVQASMLGSL LSNLLLVLGL CFIFGGYNRV 
       160        170        180        190        200
QQTFNQTAAQ TMSSLLAIAC ASLLIPAAFR ATLPHGKEDH FIDGKILELS 
       210        220        230        240        250
RGTSIVILIV YVLFLYFQLG SHHALFEQQE EETDEVMSTI SRNPHHSLSV 
       260        270        280        290        300
KSSLVILLGT TVIISFCADF LVGTIDNVVE STGLSKTFIG LIVIPIVGNA 
       310        320        330        340        350
AEHVTSVLVA MKDKMDLALG VAIGSSLQVA LFVTPFMVLV GWMIDVPMTL 
       360        370        380        390        400
NFSTFETATL FIAVFLSNYL ILDGESNWLE GVMSLAMYIL IAMAFFYYPD 
       410 
EKTLDSIGNS L

Length:411

Mass (Da):44,646

Last modified:November 1, 1996 - v1

Checksum:ⁱ4A946818A02E8078

Natural variant

Feature key	Position(s)	DescriptionActions	Length
Natural variantⁱ	204	S → A in manganese-resistant mutant 2.	1
Natural variantⁱ	208	L → P in manganese-resistant mutant 1.	1
Natural variantⁱ	383	M → I in VCX1-D1. 1 Publication	1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U18944 Genomic DNA Translation: AAC49550.1 U36603 Genomic DNA Translation: AAB60313.1 AJ001272 Genomic DNA Translation: CAA04645.1 AJ001273 Genomic DNA Translation: CAA04646.1 Z74176 Genomic DNA Translation: CAA98696.1 BK006938 Genomic DNA Translation: DAA11732.1
PIRⁱ	S61933
NCBI Reference Sequences More...RefSeqⁱ	NP_010155.1, NM_001180187.1

Genome annotation databases

EnsemblFungiⁱ	YDL128W; YDL128W; YDL128W
GeneIDⁱ	851429
KEGGⁱ	sce:YDL128W

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q99385	Vacuolar calcium ion transporter		YEASC		411	UniRef100_Q99385
UPI00032D6810		YEAST		421

Protein	Similar proteins		Species	Score	Length	Source
Q99385	Vacuolar calcium ion transporter		YEASC		411	UniRef90_Q99385
Vacuolar calcium ion transporter		SACEU		411
Vacuolar calcium ion transporter		SACAR		411
Vacuolar calcium ion transporter		YEAS1		411
Vacuolar calcium ion transporter		YEASA		411
+13

Protein	Similar proteins		Species	Score	Length	Source
Q99385	Vacuolar calcium ion transporter		YEASC		411	UniRef50_Q99385
Vacuolar calcium ion transporter		YEAS7		411
Vacuolar calcium ion transporter		YEAS2		411
Vacuolar calcium ion transporter		SACEU		411
Vacuolar calcium ion transporter		YEASK		411
+37

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U18944 Genomic DNA Translation: AAC49550.1 U36603 Genomic DNA Translation: AAB60313.1 AJ001272 Genomic DNA Translation: CAA04645.1 AJ001273 Genomic DNA Translation: CAA04646.1 Z74176 Genomic DNA Translation: CAA98696.1 BK006938 Genomic DNA Translation: DAA11732.1
PIRⁱ	S61933
RefSeqⁱ	NP_010155.1, NM_001180187.1

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	4K1C	X-ray	2.30	A/B	1-411	[»]
ProteinModelPortalⁱ	Q99385
SMRⁱ	Q99385
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	31935, 39 interactors
DIPⁱ	DIP-5039N
IntActⁱ	Q99385, 1 interactor
STRINGⁱ	4932.YDL128W

Protein family/group databases

TCDBⁱ	2.A.19.2.2 the ca(2+):cation antiporter (caca) family

TCDBⁱ

2.A.19.2.2 the ca(2+):cation antiporter (caca) family

PTM databases

iPTMnetⁱ	Q99385

iPTMnetⁱ

Q99385

Proteomic databases

MaxQBⁱ	Q99385
PaxDbⁱ	Q99385
PRIDEⁱ	Q99385

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblFungiⁱ	YDL128W; YDL128W; YDL128W
GeneIDⁱ	851429
KEGGⁱ	sce:YDL128W

Organism-specific databases

SGDⁱ	S000002286 VCX1

Phylogenomic databases

HOGENOMⁱ	HOG000083011
InParanoidⁱ	Q99385
KOⁱ	K07300
OMAⁱ	SNWLEGT
OrthoDBⁱ	EOG092C2NQI

Enzyme and pathway databases

BioCycⁱ	YEAST:G3O-29527-MONOMER

BioCycⁱ

YEAST:G3O-29527-MONOMER

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:Q99385

PROⁱ

PR:Q99385

Family and domain databases

InterProⁱ	View protein in InterPro IPR004713 CaH_exchang IPR004798 CAX IPR004837 NaCa_Exmemb
Pfamⁱ	View protein in Pfam PF01699 Na_Ca_ex, 2 hits
TIGRFAMsⁱ	TIGR00846 caca2, 1 hit TIGR00378 cax, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	VCX1_YEAST
Accessionⁱ	Q99385Primary (citable) accession number: Q99385 Secondary accession number(s): D6VRM2, O13580, O13581
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	February 7, 2006
	Last sequence update:	November 1, 1996
	Last modified:	November 7, 2018
	This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Fungal Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
PDB cross-references
Index of Protein Data Bank (PDB) cross-references
Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
Yeast chromosome IV
Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q99385 (VCX1_YEAST)

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Vacuolar calcium ion transporter

VCX1

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Vacuole

Vacuole

Other locations

Topology

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Natural variant

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ