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Protein

Vacuolar calcium ion transporter

Gene

VCX1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Has a role in promoting intracellular calcium ion sequestration via the exchange of calcium ions for hydrogen ions across the vacuolar membrane. Involved also in manganese ion homeostasis via its uptake into the vacuole.2 Publications

Miscellaneous

Present with 7770 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • calcium:proton antiporter activity Source: SGD
  • metal ion binding Source: UniProtKB-KW
  • potassium:proton antiporter activity Source: SGD

GO - Biological processi

Keywordsi

Biological processCalcium transport, Ion transport, Transport
LigandCalcium, Manganese, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29527-MONOMER

Protein family/group databases

TCDBi2.A.19.2.2 the ca(2+):cation antiporter (caca) family

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar calcium ion transporter
Alternative name(s):
High copy number undoes manganese protein 1
Manganese resistance 1 protein
Vacuolar Ca(2+)/H(+) exchanger
Gene namesi
Name:VCX1
Synonyms:HUM1, MNR1
Ordered Locus Names:YDL128W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

SGDiS000002286 VCX1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 30CytoplasmicSequence analysisAdd BLAST30
Transmembranei31 – 53HelicalSequence analysisAdd BLAST23
Topological domaini54 – 59VacuolarSequence analysis6
Transmembranei60 – 82HelicalSequence analysisAdd BLAST23
Topological domaini83 – 91CytoplasmicSequence analysis9
Transmembranei92 – 114HelicalSequence analysisAdd BLAST23
Topological domaini115 – 126VacuolarSequence analysisAdd BLAST12
Transmembranei127 – 146HelicalSequence analysisAdd BLAST20
Topological domaini147 – 160CytoplasmicSequence analysisAdd BLAST14
Transmembranei161 – 183HelicalSequence analysisAdd BLAST23
Topological domaini184 – 203VacuolarSequence analysisAdd BLAST20
Transmembranei204 – 226HelicalSequence analysisAdd BLAST23
Topological domaini227 – 252CytoplasmicSequence analysisAdd BLAST26
Transmembranei253 – 275HelicalSequence analysisAdd BLAST23
Topological domaini276 – 287VacuolarSequence analysisAdd BLAST12
Transmembranei288 – 310HelicalSequence analysisAdd BLAST23
Topological domaini311 – 319CytoplasmicSequence analysis9
Transmembranei320 – 342HelicalSequence analysisAdd BLAST23
Topological domaini343 – 348VacuolarSequence analysis6
Transmembranei349 – 371HelicalSequence analysisAdd BLAST23
Topological domaini372 – 380CytoplasmicSequence analysis9
Transmembranei381 – 398HelicalSequence analysisAdd BLAST18
Topological domaini399 – 411VacuolarSequence analysisAdd BLAST13

Keywords - Cellular componenti

Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002095041 – 411Vacuolar calcium ion transporterAdd BLAST411

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei13PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ99385
PaxDbiQ99385
PRIDEiQ99385

PTM databases

iPTMnetiQ99385

Interactioni

Protein-protein interaction databases

BioGridi31935, 39 interactors
DIPiDIP-5039N
IntActiQ99385, 1 interactor
STRINGi4932.YDL128W

Structurei

Secondary structure

1411
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ99385
SMRiQ99385
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000083011
InParanoidiQ99385
KOiK07300
OMAiSNWLEGT
OrthoDBiEOG092C2NQI

Family and domain databases

InterProiView protein in InterPro
IPR004713 CaH_exchang
IPR004798 CAX
IPR004837 NaCa_Exmemb
PfamiView protein in Pfam
PF01699 Na_Ca_ex, 2 hits
TIGRFAMsiTIGR00846 caca2, 1 hit
TIGR00378 cax, 1 hit

Sequencei

Sequence statusi: Complete.

Q99385-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDATTPLLTV ANSHPARNPK HTAWRAAVYD LQYILKASPL NFLLVFVPLG
60 70 80 90 100
LIWGHFQLSH TLTFLFNFLA IIPLAAILAN ATEELADKAG NTIGGLLNAT
110 120 130 140 150
FGNAVELIVS IIALKKGQVR IVQASMLGSL LSNLLLVLGL CFIFGGYNRV
160 170 180 190 200
QQTFNQTAAQ TMSSLLAIAC ASLLIPAAFR ATLPHGKEDH FIDGKILELS
210 220 230 240 250
RGTSIVILIV YVLFLYFQLG SHHALFEQQE EETDEVMSTI SRNPHHSLSV
260 270 280 290 300
KSSLVILLGT TVIISFCADF LVGTIDNVVE STGLSKTFIG LIVIPIVGNA
310 320 330 340 350
AEHVTSVLVA MKDKMDLALG VAIGSSLQVA LFVTPFMVLV GWMIDVPMTL
360 370 380 390 400
NFSTFETATL FIAVFLSNYL ILDGESNWLE GVMSLAMYIL IAMAFFYYPD
410
EKTLDSIGNS L
Length:411
Mass (Da):44,646
Last modified:November 1, 1996 - v1
Checksum:i4A946818A02E8078
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti204S → A in manganese-resistant mutant 2. 1
Natural varianti208L → P in manganese-resistant mutant 1. 1
Natural varianti383M → I in VCX1-D1. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18944 Genomic DNA Translation: AAC49550.1
U36603 Genomic DNA Translation: AAB60313.1
AJ001272 Genomic DNA Translation: CAA04645.1
AJ001273 Genomic DNA Translation: CAA04646.1
Z74176 Genomic DNA Translation: CAA98696.1
BK006938 Genomic DNA Translation: DAA11732.1
PIRiS61933
RefSeqiNP_010155.1, NM_001180187.1

Genome annotation databases

EnsemblFungiiYDL128W; YDL128W; YDL128W
GeneIDi851429
KEGGisce:YDL128W

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18944 Genomic DNA Translation: AAC49550.1
U36603 Genomic DNA Translation: AAB60313.1
AJ001272 Genomic DNA Translation: CAA04645.1
AJ001273 Genomic DNA Translation: CAA04646.1
Z74176 Genomic DNA Translation: CAA98696.1
BK006938 Genomic DNA Translation: DAA11732.1
PIRiS61933
RefSeqiNP_010155.1, NM_001180187.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4K1CX-ray2.30A/B1-411[»]
ProteinModelPortaliQ99385
SMRiQ99385
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31935, 39 interactors
DIPiDIP-5039N
IntActiQ99385, 1 interactor
STRINGi4932.YDL128W

Protein family/group databases

TCDBi2.A.19.2.2 the ca(2+):cation antiporter (caca) family

PTM databases

iPTMnetiQ99385

Proteomic databases

MaxQBiQ99385
PaxDbiQ99385
PRIDEiQ99385

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDL128W; YDL128W; YDL128W
GeneIDi851429
KEGGisce:YDL128W

Organism-specific databases

SGDiS000002286 VCX1

Phylogenomic databases

HOGENOMiHOG000083011
InParanoidiQ99385
KOiK07300
OMAiSNWLEGT
OrthoDBiEOG092C2NQI

Enzyme and pathway databases

BioCyciYEAST:G3O-29527-MONOMER

Miscellaneous databases

PROiPR:Q99385

Family and domain databases

InterProiView protein in InterPro
IPR004713 CaH_exchang
IPR004798 CAX
IPR004837 NaCa_Exmemb
PfamiView protein in Pfam
PF01699 Na_Ca_ex, 2 hits
TIGRFAMsiTIGR00846 caca2, 1 hit
TIGR00378 cax, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiVCX1_YEAST
AccessioniPrimary (citable) accession number: Q99385
Secondary accession number(s): D6VRM2, O13580, O13581
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: November 1, 1996
Last modified: November 7, 2018
This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names
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Main funding by: National Institutes of Health

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