We will be switching to the new UniProt website soon. Please explore and share your feedback.
Take me to the new website.
UniProtKB - Q99385 (VCX1_YEAST)
Protein
Vacuolar calcium ion transporter
Gene
VCX1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Has a role in promoting intracellular calcium ion sequestration via the exchange of calcium ions for hydrogen ions across the vacuolar membrane. Involved also in manganese ion homeostasis via its uptake into the vacuole.
2 PublicationsMiscellaneous
Present with 7770 molecules/cell in log phase SD medium.1 Publication
GO - Molecular functioni
- calcium:proton antiporter activity Source: SGD
- metal ion binding Source: UniProtKB-KW
- potassium:proton antiporter activity Source: SGD
GO - Biological processi
- calcium ion transmembrane transport Source: GO_Central
- calcium ion transport Source: SGD
- cellular calcium ion homeostasis Source: SGD
- transmembrane transport Source: SGD
Keywordsi
Biological process | Calcium transport, Ion transport, Transport |
Ligand | Calcium, Manganese, Metal-binding |
Protein family/group databases
TCDBi | 2.A.19.2.2, the ca(2+):cation antiporter (caca) family |
Names & Taxonomyi
Protein namesi | Recommended name: Vacuolar calcium ion transporterAlternative name(s): High copy number undoes manganese protein 1 Manganese resistance 1 protein Vacuolar Ca(2+)/H(+) exchanger |
Gene namesi | Name:VCX1 Synonyms:HUM1, MNR1 Ordered Locus Names:YDL128W |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000002286, VCX1 |
VEuPathDBi | FungiDB:YDL128W |
Subcellular locationi
Vacuole
- Vacuole membrane 1 Publication; Multi-pass membrane protein 1 Publication
Vacuole
- fungal-type vacuole Source: SGD
- fungal-type vacuole membrane Source: SGD
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 30 | Cytoplasmic1 PublicationAdd BLAST | 30 | |
Transmembranei | 31 – 53 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 54 – 59 | Vacuolar1 Publication | 6 | |
Transmembranei | 60 – 82 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 83 – 91 | Cytoplasmic1 Publication | 9 | |
Transmembranei | 92 – 114 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 115 – 126 | Vacuolar1 PublicationAdd BLAST | 12 | |
Transmembranei | 127 – 146 | HelicalSequence analysisAdd BLAST | 20 | |
Topological domaini | 147 – 160 | Cytoplasmic1 PublicationAdd BLAST | 14 | |
Transmembranei | 161 – 183 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 184 – 203 | Vacuolar1 PublicationAdd BLAST | 20 | |
Transmembranei | 204 – 226 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 227 – 252 | Cytoplasmic1 PublicationAdd BLAST | 26 | |
Transmembranei | 253 – 275 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 276 – 287 | Vacuolar1 PublicationAdd BLAST | 12 | |
Transmembranei | 288 – 310 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 311 – 319 | Cytoplasmic1 Publication | 9 | |
Transmembranei | 320 – 342 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 343 – 348 | Vacuolar1 Publication | 6 | |
Transmembranei | 349 – 371 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 372 – 380 | Cytoplasmic1 Publication | 9 | |
Transmembranei | 381 – 398 | HelicalSequence analysisAdd BLAST | 18 | |
Topological domaini | 399 – 411 | Vacuolar1 PublicationAdd BLAST | 13 |
Keywords - Cellular componenti
Membrane, VacuolePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000209504 | 1 – 411 | Vacuolar calcium ion transporterAdd BLAST | 411 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineCombined sources | 1 | |
Modified residuei | 13 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
MaxQBi | Q99385 |
PaxDbi | Q99385 |
PRIDEi | Q99385 |
PTM databases
iPTMneti | Q99385 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 31935, 44 interactors |
DIPi | DIP-5039N |
IntActi | Q99385, 1 interactor |
STRINGi | 4932.YDL128W |
Miscellaneous databases
RNActi | Q99385, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q99385 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1397, Eukaryota |
GeneTreei | ENSGT00390000016897 |
HOGENOMi | CLU_008721_4_2_1 |
InParanoidi | Q99385 |
OMAi | AAVMITC |
Family and domain databases
Gene3Di | 1.20.1420.30, 1 hit |
InterProi | View protein in InterPro IPR004713, CaH_exchang IPR004798, CAX-like IPR004837, NaCa_Exmemb IPR044880, NCX_ion-bd_dom_sf |
PANTHERi | PTHR31503, PTHR31503, 1 hit |
Pfami | View protein in Pfam PF01699, Na_Ca_ex, 2 hits |
TIGRFAMsi | TIGR00378, cax, 1 hit |
i Sequence
Sequence statusi: Complete.
Q99385-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDATTPLLTV ANSHPARNPK HTAWRAAVYD LQYILKASPL NFLLVFVPLG
60 70 80 90 100
LIWGHFQLSH TLTFLFNFLA IIPLAAILAN ATEELADKAG NTIGGLLNAT
110 120 130 140 150
FGNAVELIVS IIALKKGQVR IVQASMLGSL LSNLLLVLGL CFIFGGYNRV
160 170 180 190 200
QQTFNQTAAQ TMSSLLAIAC ASLLIPAAFR ATLPHGKEDH FIDGKILELS
210 220 230 240 250
RGTSIVILIV YVLFLYFQLG SHHALFEQQE EETDEVMSTI SRNPHHSLSV
260 270 280 290 300
KSSLVILLGT TVIISFCADF LVGTIDNVVE STGLSKTFIG LIVIPIVGNA
310 320 330 340 350
AEHVTSVLVA MKDKMDLALG VAIGSSLQVA LFVTPFMVLV GWMIDVPMTL
360 370 380 390 400
NFSTFETATL FIAVFLSNYL ILDGESNWLE GVMSLAMYIL IAMAFFYYPD
410
EKTLDSIGNS L
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 204 | S → A in manganese-resistant mutant 2. | 1 | |
Natural varianti | 208 | L → P in manganese-resistant mutant 1. | 1 | |
Natural varianti | 383 | M → I in VCX1-D1. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U18944 Genomic DNA Translation: AAC49550.1 U36603 Genomic DNA Translation: AAB60313.1 AJ001272 Genomic DNA Translation: CAA04645.1 AJ001273 Genomic DNA Translation: CAA04646.1 Z74176 Genomic DNA Translation: CAA98696.1 BK006938 Genomic DNA Translation: DAA11732.1 |
PIRi | S61933 |
RefSeqi | NP_010155.1, NM_001180187.1 |
Genome annotation databases
EnsemblFungii | YDL128W_mRNA; YDL128W; YDL128W |
GeneIDi | 851429 |
KEGGi | sce:YDL128W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U18944 Genomic DNA Translation: AAC49550.1 U36603 Genomic DNA Translation: AAB60313.1 AJ001272 Genomic DNA Translation: CAA04645.1 AJ001273 Genomic DNA Translation: CAA04646.1 Z74176 Genomic DNA Translation: CAA98696.1 BK006938 Genomic DNA Translation: DAA11732.1 |
PIRi | S61933 |
RefSeqi | NP_010155.1, NM_001180187.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4K1C | X-ray | 2.30 | A/B | 1-411 | [»] | |
SMRi | Q99385 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 31935, 44 interactors |
DIPi | DIP-5039N |
IntActi | Q99385, 1 interactor |
STRINGi | 4932.YDL128W |
Protein family/group databases
TCDBi | 2.A.19.2.2, the ca(2+):cation antiporter (caca) family |
PTM databases
iPTMneti | Q99385 |
Proteomic databases
MaxQBi | Q99385 |
PaxDbi | Q99385 |
PRIDEi | Q99385 |
Genome annotation databases
EnsemblFungii | YDL128W_mRNA; YDL128W; YDL128W |
GeneIDi | 851429 |
KEGGi | sce:YDL128W |
Organism-specific databases
SGDi | S000002286, VCX1 |
VEuPathDBi | FungiDB:YDL128W |
Phylogenomic databases
eggNOGi | KOG1397, Eukaryota |
GeneTreei | ENSGT00390000016897 |
HOGENOMi | CLU_008721_4_2_1 |
InParanoidi | Q99385 |
OMAi | AAVMITC |
Miscellaneous databases
PROi | PR:Q99385 |
RNActi | Q99385, protein |
Family and domain databases
Gene3Di | 1.20.1420.30, 1 hit |
InterProi | View protein in InterPro IPR004713, CaH_exchang IPR004798, CAX-like IPR004837, NaCa_Exmemb IPR044880, NCX_ion-bd_dom_sf |
PANTHERi | PTHR31503, PTHR31503, 1 hit |
Pfami | View protein in Pfam PF01699, Na_Ca_ex, 2 hits |
TIGRFAMsi | TIGR00378, cax, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | VCX1_YEAST | |
Accessioni | Q99385Primary (citable) accession number: Q99385 Secondary accession number(s): D6VRM2, O13580, O13581 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 7, 2006 |
Last sequence update: | November 1, 1996 | |
Last modified: | February 23, 2022 | |
This is version 146 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome IV
Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families