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Protein

Thermolabile hemolysin

Gene

VPA0226

Organism
Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Phospholipase hydrolyzing both fatty acid esters of phospholipid, i.e. it hydrolyzes phosphatidylcholine (PC) to lysophosphatidylcholine (LPC) and then LPC to glycerophosphorylcholine (GPC).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei153NucleophileBy similarity1
Active sitei390By similarity1
Active sitei393By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Toxin
Biological processCytolysis, Hemolysis, Lipid degradation, Lipid metabolism

Enzyme and pathway databases

BioCyciVPAR223926:G1GTB-3457-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Thermolabile hemolysin
Short name:
TL
Alternative name(s):
Atypical phospholipase
Lecithin-dependent hemolysin
Short name:
LDH
Lysophospholipase
Phospholipase A2
Gene namesi
Ordered Locus Names:VPA0226
OrganismiVibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633)
Taxonomic identifieri223926 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
Proteomesi
  • UP000002493 Componenti: Chromosome 2

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 191 PublicationAdd BLAST19
ChainiPRO_000001784920 – 418Thermolabile hemolysinAdd BLAST399

Post-translational modificationi

There are two forms of LDH. The LDH(S) may be a protein in which 13 residues of the N-terminal of LDH(L) are deleted.

Interactioni

Protein-protein interaction databases

STRINGi223926.VPA0226

Structurei

3D structure databases

ProteinModelPortaliQ99289
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the 'GDSL' lipolytic enzyme family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105KUU Bacteria
COG3240 LUCA
HOGENOMiHOG000218229
KOiK11018
OMAiVHWIGAN

Family and domain databases

Gene3Di3.40.50.1110, 1 hit
InterProiView protein in InterPro
IPR001087 GDSL
IPR008265 Lipase_GDSL_AS
IPR036514 SGNH_hydro_sf
PfamiView protein in Pfam
PF00657 Lipase_GDSL, 1 hit
PROSITEiView protein in PROSITE
PS01098 LIPASE_GDSL_SER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99289-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MMKKTITLLT ALLPLASAVA EEPTLSPEMV SASEVISTQE NQTYTYVRCW
60 70 80 90 100
YRTSYSKDDP ATDWEWAKNE DGSYFTIDGY WWSSVSFKNM FYTNTSQNVI
110 120 130 140 150
RQRCEATLDL ANENADITFF AADNRFSYNH TIWSNDAAMQ PDQINKVVAL
160 170 180 190 200
GDSLSDTGNI FNASQWRFPN PNSWFLGHFS NGFVWTEYIA KAKNLPLYNW
210 220 230 240 250
AVGGAAGENQ YIALTGVGEQ VSSYLTYAKL AKNYKPANTL FTLEFGLNDF
260 270 280 290 300
MNYNRGVPEV KADYAEALIR LTDAGAKNFM LMTLPDATKA PQFKYSTQEE
310 320 330 340 350
IDKIRAKVLE MNEFIKAQAM YYKAQGYNIT LFDTHALFET LTSAPEEHGF
360 370 380 390 400
VNASDPCLDI NRSSSVDYMY THALRSECAA SGAEKFVFWD VTHPTTATHR
410
YVAEKMLESS NNLAEYRF
Length:418
Mass (Da):47,392
Last modified:April 4, 2003 - v2
Checksum:iDC59A641DD04B1BD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti20A → T AA sequence (PubMed:1791426).Curated1
Sequence conflicti219E → D in AAA27526 (PubMed:3508495).Curated1
Sequence conflicti390D → N in AAA27526 (PubMed:3508495).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti20 – 32Missing in LDH(S). Add BLAST13

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36437 Genomic DNA Translation: AAA27526.1
BA000032 Genomic DNA Translation: BAC61569.1
PIRiA53888
RefSeqiNP_799736.1, NC_004605.1
WP_005481504.1, NC_004605.1

Genome annotation databases

EnsemblBacteriaiBAC61569; BAC61569; BAC61569
GeneIDi1190914
KEGGivpa:VPA0226
PATRICifig|223926.6.peg.3181

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36437 Genomic DNA Translation: AAA27526.1
BA000032 Genomic DNA Translation: BAC61569.1
PIRiA53888
RefSeqiNP_799736.1, NC_004605.1
WP_005481504.1, NC_004605.1

3D structure databases

ProteinModelPortaliQ99289
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi223926.VPA0226

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC61569; BAC61569; BAC61569
GeneIDi1190914
KEGGivpa:VPA0226
PATRICifig|223926.6.peg.3181

Phylogenomic databases

eggNOGiENOG4105KUU Bacteria
COG3240 LUCA
HOGENOMiHOG000218229
KOiK11018
OMAiVHWIGAN

Enzyme and pathway databases

BioCyciVPAR223926:G1GTB-3457-MONOMER

Family and domain databases

Gene3Di3.40.50.1110, 1 hit
InterProiView protein in InterPro
IPR001087 GDSL
IPR008265 Lipase_GDSL_AS
IPR036514 SGNH_hydro_sf
PfamiView protein in Pfam
PF00657 Lipase_GDSL, 1 hit
PROSITEiView protein in PROSITE
PS01098 LIPASE_GDSL_SER, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiHLT_VIBPA
AccessioniPrimary (citable) accession number: Q99289
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: April 4, 2003
Last modified: November 7, 2018
This is version 104 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health

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