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Protein

Endoplasmic reticulum chaperone BiP

Gene

KAR2

Organism
Yarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER. Is required for secretory polypeptide translocation. May physically associate with SEC63 protein in the endoplasmic reticulum and this interaction may be regulated by ATP hydrolysis.By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.By similarity

Activity regulationi

The chaperone activity is regulated by ATP-induced allosteric coupling of the nucleotide-binding (NBD) and substrate-binding (SBD) domains. In the ADP-bound and nucleotide-free (apo) states, the two domains have little interaction. In contrast, in the ATP-bound state the two domains are tightly coupled, which results in drastically accelerated kinetics in both binding and release of polypeptide substrates. J domain-containing co-chaperones stimulate the ATPase activity and are required for efficient substrate recognition.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei104ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi45 – 48ATPBy similarity4
Nucleotide bindingi234 – 236ATPBy similarity3
Nucleotide bindingi300 – 307ATPBy similarity8
Nucleotide bindingi371 – 374ATPBy similarity4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChaperone, Hydrolase
Biological processStress response
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Endoplasmic reticulum chaperone BiPCurated (EC:3.6.4.10By similarity)
Alternative name(s):
Immunoglobulin heavy chain-binding protein homologCurated
Short name:
BiPCurated
Gene namesi
Name:KAR2
Ordered Locus Names:YALI0E13706g
OrganismiYarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica)
Taxonomic identifieri284591 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDipodascaceaeYarrowia
Proteomesi
  • UP000001300 Componenti: Chromosome E

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 26Sequence analysisAdd BLAST26
ChainiPRO_000001358627 – 670Endoplasmic reticulum chaperone BiPAdd BLAST644

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi464N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiQ99170

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ99170, 1 interactor
MINTiQ99170
STRINGi4952.XP_503913.1

Structurei

3D structure databases

ProteinModelPortaliQ99170
SMRiQ99170
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni133 – 287Nucleotide-binding (NBD)By similarityAdd BLAST155
Regioni407 – 507Substrate-binding (SBD)By similarityAdd BLAST101

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi667 – 670Prevents secretion from ERPROSITE-ProRule annotation4

Sequence similaritiesi

Belongs to the heat shock protein 70 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000228135
InParanoidiQ99170
KOiK09490
OMAiCVGVMQK
OrthoDBiEOG092C1VPN

Family and domain databases

Gene3Di1.20.1270.10, 1 hit
2.60.34.10, 1 hit
InterProiView protein in InterPro
IPR018181 Heat_shock_70_CS
IPR029048 HSP70_C_sf
IPR029047 HSP70_peptide-bd_sf
IPR013126 Hsp_70_fam
PANTHERiPTHR19375 PTHR19375, 1 hit
PfamiView protein in Pfam
PF00012 HSP70, 1 hit
PRINTSiPR00301 HEATSHOCK70
SUPFAMiSSF100920 SSF100920, 1 hit
SSF100934 SSF100934, 1 hit
PROSITEiView protein in PROSITE
PS00014 ER_TARGET, 1 hit
PS00297 HSP70_1, 1 hit
PS00329 HSP70_2, 1 hit
PS01036 HSP70_3, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99170-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKFSMPSWGV VFYALLVCLL PFLSKAGVQA DDVDSYGTVI GIDLGTTYSC
60 70 80 90 100
VGVMKGGRVE ILANDQGSRI TPSYVAFTED ERLVGDAAKN QAANNPFNTI
110 120 130 140 150
FDIKRLIGLK YKDESVQRDI KHFPYKVKNK DGKPVVVVET KGEKKTYTPE
160 170 180 190 200
EISAMILTKM KDIAQDYLGK KVTHAVVTVP AYFNDAQRQA TKDAGIIAGL
210 220 230 240 250
NVLRIVNEPT AAAIAYGLDH TDDEKQIVVY DLGGGTFDVS LLSIESGVFE
260 270 280 290 300
VLATAGDTHL GGEDFDYRVI KHFVKQYNKK HDVDITKNAK TIGKLKREVE
310 320 330 340 350
KAKRTLSSQM STRIEIESFF DGEDFSETLT RAKFEELNID LFKRTLKPVE
360 370 380 390 400
QVLKDSGVKK EDVHDIVLVG GSTRIPKVQE LLEKFFDGKK ASKGINPDEA
410 420 430 440 450
VAYGAAVQAG VLSGEDGVED IVLLDVNPLT LGIETTGGVM TKLINRNTNI
460 470 480 490 500
PTKKSQIFST AVDNQSTVLI QVFEGERTMS KDNNLLGKFE LKGIPPAPRG
510 520 530 540 550
VPQIEVTFEL DANGILRVTA HDKGTGKSET ITITNDKGRL SKDEIERMVE
560 570 580 590 600
EAERFAEEDA LIRETIEAKN SLENYAHSLR NQVADKSGLG GKISADDKEA
610 620 630 640 650
LNDAVTETLE WLEANSVSAT KEDFEEKKEA LSAIAYPITS KIYEGGEGGD
660 670
ESNDGGFYAD DDEAPFHDEL
Length:670
Mass (Da):73,524
Last modified:November 1, 1997 - v1
Checksum:i2C2DFB436BCC9C0E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U63136 Genomic DNA Translation: AAC49736.1
CR382131 Genomic DNA Translation: CAG79506.1
RefSeqiXP_503913.1, XM_503913.1

Genome annotation databases

EnsemblFungiiCAG79506; CAG79506; YALI0_E13706g
GeneIDi2912786
KEGGiyli:YALI0E13706g

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U63136 Genomic DNA Translation: AAC49736.1
CR382131 Genomic DNA Translation: CAG79506.1
RefSeqiXP_503913.1, XM_503913.1

3D structure databases

ProteinModelPortaliQ99170
SMRiQ99170
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ99170, 1 interactor
MINTiQ99170
STRINGi4952.XP_503913.1

Proteomic databases

PRIDEiQ99170

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCAG79506; CAG79506; YALI0_E13706g
GeneIDi2912786
KEGGiyli:YALI0E13706g

Phylogenomic databases

HOGENOMiHOG000228135
InParanoidiQ99170
KOiK09490
OMAiCVGVMQK
OrthoDBiEOG092C1VPN

Family and domain databases

Gene3Di1.20.1270.10, 1 hit
2.60.34.10, 1 hit
InterProiView protein in InterPro
IPR018181 Heat_shock_70_CS
IPR029048 HSP70_C_sf
IPR029047 HSP70_peptide-bd_sf
IPR013126 Hsp_70_fam
PANTHERiPTHR19375 PTHR19375, 1 hit
PfamiView protein in Pfam
PF00012 HSP70, 1 hit
PRINTSiPR00301 HEATSHOCK70
SUPFAMiSSF100920 SSF100920, 1 hit
SSF100934 SSF100934, 1 hit
PROSITEiView protein in PROSITE
PS00014 ER_TARGET, 1 hit
PS00297 HSP70_1, 1 hit
PS00329 HSP70_2, 1 hit
PS01036 HSP70_3, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiBIP_YARLI
AccessioniPrimary (citable) accession number: Q99170
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 7, 2018
This is version 110 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health

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