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Protein

Unconventional myosin-Va

Gene

Myo5a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Processive actin-based motor that can move in large steps approximating the 36-nm pseudo-repeat of the actin filament. Involved in melanosome transport. Also mediates the transport of vesicles to the plasma membrane. May also be required for some polarization process involved in dendrite formation.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi163 – 170ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActin-binding, Calmodulin-binding, Motor protein, Myosin
Biological processProtein transport, Transport
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-2029482 Regulation of actin dynamics for phagocytic cup formation

Names & Taxonomyi

Protein namesi
Recommended name:
Unconventional myosin-Va
Alternative name(s):
Dilute myosin heavy chain, non-muscle
Gene namesi
Name:Myo5a
Synonyms:Dilute
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:105976 Myo5a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi1528R → H: Slightly reduces affinity for MLPH. 1 Publication1
Mutagenesisi1535I → E: Strongly reduces affinity for MLPH and SYTL4. 1 Publication1
Mutagenesisi1539K → E: Strongly reduces affinity for MLPH and SYTL4. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001234572 – 1853Unconventional myosin-VaAdd BLAST1852

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei600PhosphoserineCombined sources1
Modified residuei1032PhosphothreonineBy similarity1
Modified residuei1450PhosphoserineCombined sources1
Modified residuei1650PhosphoserineCombined sources1
Modified residuei1758PhosphothreonineSequence analysis1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ99104
MaxQBiQ99104
PaxDbiQ99104
PeptideAtlasiQ99104
PRIDEiQ99104

PTM databases

iPTMnetiQ99104
PhosphoSitePlusiQ99104
SwissPalmiQ99104

Expressioni

Tissue specificityi

Detected in melanocytes.

Gene expression databases

BgeeiENSMUSG00000034593 Expressed in 270 organ(s), highest expression level in medial geniculate body
CleanExiMM_MYO5A
ExpressionAtlasiQ99104 baseline and differential
GenevisibleiQ99104 MM

Interactioni

Subunit structurei

May be a homodimer, which associates with multiple calmodulin or myosin light chains (PubMed:17151196). Interacts with RIPL2, the interaction is required for its role in dendrite formation (PubMed:23798443). Interacts with MLPH (PubMed:11887186). Interacts with SYTL4 (PubMed:23798443). Interacts with MYRIP (PubMed:12221080). Interacts with RAB10; mediates the transport to the plasma membrane of SLC2A4/GLUT4 storage vesicles (PubMed:22908308). Interacts with FMR1; this interaction occurs in association with polyribosome (PubMed:12147688).6 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-400199,EBI-400199

GO - Molecular functioni

Protein-protein interaction databases

BioGridi201666, 22 interactors
CORUMiQ99104
DIPiDIP-29542N
IntActiQ99104, 21 interactors
MINTiQ99104
STRINGi10090.ENSMUSP00000116028

Structurei

Secondary structure

11853
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ99104
SMRiQ99104
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ99104

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 60Myosin N-terminal SH3-likePROSITE-ProRule annotationAdd BLAST53
Domaini69 – 763Myosin motorPROSITE-ProRule annotationAdd BLAST695
Domaini766 – 788IQ 1PROSITE-ProRule annotationAdd BLAST23
Domaini789 – 813IQ 2PROSITE-ProRule annotationAdd BLAST25
Domaini814 – 836IQ 3PROSITE-ProRule annotationAdd BLAST23
Domaini837 – 861IQ 4PROSITE-ProRule annotationAdd BLAST25
Domaini862 – 884IQ 5PROSITE-ProRule annotationAdd BLAST23
Domaini885 – 913IQ 6PROSITE-ProRule annotationAdd BLAST29
Domaini1532 – 1808DilutePROSITE-ProRule annotationAdd BLAST277

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni643 – 665Actin-bindingSequence analysisAdd BLAST23

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili914 – 1237Sequence analysisAdd BLAST324
Coiled coili1314 – 1443Sequence analysisAdd BLAST130

Sequence similaritiesi

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG0160 Eukaryota
KOG0161 Eukaryota
COG5022 LUCA
GeneTreeiENSGT00900000140810
HOGENOMiHOG000171839
HOVERGENiHBG052556
InParanoidiQ99104
KOiK10357
TreeFamiTF328771

Family and domain databases

CDDicd15478 Myo5a_CBD, 1 hit
cd01380 MYSc_Myo5, 1 hit
Gene3Di2.30.30.360, 1 hit
3.40.850.10, 3 hits
InterProiView protein in InterPro
IPR002710 Dilute_dom
IPR000048 IQ_motif_EF-hand-BS
IPR036961 Kinesin_motor_dom_sf
IPR037988 Myo5a_CBD
IPR001609 Myosin_head_motor_dom
IPR004009 Myosin_N
IPR008989 Myosin_S1_N
IPR036103 MYSc_Myo5
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF01843 DIL, 1 hit
PF00612 IQ, 6 hits
PF00063 Myosin_head, 1 hit
PRINTSiPR00193 MYOSINHEAVY
SMARTiView protein in SMART
SM01132 DIL, 1 hit
SM00015 IQ, 6 hits
SM00242 MYSc, 1 hit
SUPFAMiSSF52540 SSF52540, 2 hits
PROSITEiView protein in PROSITE
PS51126 DILUTE, 1 hit
PS50096 IQ, 6 hits
PS51456 MYOSIN_MOTOR, 1 hit
PS51844 SH3_LIKE, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 6 potential isoforms that are computationally mapped.Show allAlign All

Q99104-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAASELYTKF ARVWIPDPEE VWKSAELLKD YKPGDKVLLL HLEEGKDLEY
60 70 80 90 100
RLDPKTGELP HLRNPDILVG ENDLTALSYL HEPAVLHNLR VRFIDSKLIY
110 120 130 140 150
TYCGIVLVAI NPYEQLPIYG EDIINAYSGQ NMGDMDPHIF AVAEEAYKQM
160 170 180 190 200
ARDERNQSII VSGESGAGKT VSAKYAMRYF ATVSGSASEA NVEEKVLASN
210 220 230 240 250
PIMESIGNAK TTRNDNSSRF GKYIEIGFDK RYRIIGANMR TYLLEKSRVV
260 270 280 290 300
FQAEEERNYH IFYQLCASAK LPEFKMLRLG NADSFHYTKQ GGSPMIEGVD
310 320 330 340 350
DAKEMAHTRQ ACTLLGISES YQMGIFRILA GILHLGNVGF ASRDSDSCTI
360 370 380 390 400
PPKHEPLTIF CDLMGVDYEE MCHWLCHRKL ATATETYIKP ISKLQATNAR
410 420 430 440 450
DALAKHIYAK LFNWIVDHVN QALHSAVKQH SFIGVLDIYG FETFEINSFE
460 470 480 490 500
QFCINYANEK LQQQFNMHVF KLEQEEYMKE QIPWTLIDFY DNQPCINLIE
510 520 530 540 550
SKLGILDLLD EECKMPKGTD DTWAQKLYNT HLNKCALFEK PRMSNKAFII
560 570 580 590 600
KHFADKVEYQ CEGFLEKNKD TVFEEQIKVL KSSKFKMLPE LFQDDEKAIS
610 620 630 640 650
PTSATSSGRT PLTRVPVKPT KGRPGQTAKE HKKTVGHQFR NSLHLLMETL
660 670 680 690 700
NATTPHYVRC IKPNDFKFPF TFDEKRAVQQ LRACGVLETI RISAAGFPSR
710 720 730 740 750
WTYQEFFSRY RVLMKQKDVL GDRKQTCKNV LEKLILDKDK YQFGKTKIFF
760 770 780 790 800
RAGQVAYLEK LRADKLRAAC IRIQKTIRGW LLRKRYLCMQ RAAITVQRYV
810 820 830 840 850
RGYQARCYAK FLRRTKAATT IQKYWRMYVV RRRYKIRRAA TIVIQSYLRG
860 870 880 890 900
YLTRNRYRKI LREYKAVIIQ KRVRGWLART HYKRTMKAIV YLQCCFRRMM
910 920 930 940 950
AKRELKKLKI EARSVERYKK LHIGMENKIM QLQRKVDEQN KDYKCLMEKL
960 970 980 990 1000
TNLEGVYNSE TEKLRNDVER LQLSEEEAKV ATGRVLSLQE EIAKLRKDLE
1010 1020 1030 1040 1050
QTRSEKKSIE ERADKYKQET DQLVSNLKEE NTLLKQEKET LNHRIVEQAK
1060 1070 1080 1090 1100
EMTETMERKL VEETKQLELD LNDERLRYQN LLNEFSRLEE RYDDLKEEMT
1110 1120 1130 1140 1150
LMLNVPKPGH KRTDSTHSSN ESEYTFSSEF AETEDIAPRT EEPIEKKVPL
1160 1170 1180 1190 1200
DMSLFLKLQK RVTELEQEKQ LMQDELDRKE EQVFRSKAKE EERPQIRGAE
1210 1220 1230 1240 1250
LEYESLKRQE LESENKKLKN ELNELRKALS EKSAPEVTAP GAPAYRVLME
1260 1270 1280 1290 1300
QLTSVSEELD VRKEEVLILR SQLVSQKEAI QPKDDKNTMT DSTILLEDVQ
1310 1320 1330 1340 1350
KMKDKGEIAQ AYIGLKETNR LLESQLQSQK RSHENEAEAL RGEIQSLKEE
1360 1370 1380 1390 1400
NNRQQQLLAQ NLQLPPEARI EASLQHEITR LTNENLYFEE LYADDPKKYQ
1410 1420 1430 1440 1450
SYRISLYKRM IDLMEQLEKQ DKTVRKLKKQ LKVFAKKIGE LEVGQMENIS
1460 1470 1480 1490 1500
PGQIIDEPIR PVNIPRKEKD FQGMLEYKRE DEQKLVKNLI LELKPRGVAV
1510 1520 1530 1540 1550
NLIPGLPAYI LFMCVRHADY LNDDQKVRSL LTSTINSIKK VLKKRGDDFE
1560 1570 1580 1590 1600
TVSFWLSNTC RFLHCLKQYS GEEGFMKHNT SRQNEHCLTN FDLAEYRQVL
1610 1620 1630 1640 1650
SDLAIQIYQQ LVRVLENILQ PMIVSGMLEH ETIQGVSGVK PTGLRKRTSS
1660 1670 1680 1690 1700
IADEGTYTLD SILRQLNSFH SVMCQHGMDP ELIKQVVKQM FYIVGAITLN
1710 1720 1730 1740 1750
NLLLRKDMCS WSKGMQIRYN VSQLEEWLRD KNLMNSGAKE TLEPLIQAAQ
1760 1770 1780 1790 1800
LLQVKKKTDD DAEAICSMCN ALTTAQIVKV LNLYTPVNEF EERVSVSFIR
1810 1820 1830 1840 1850
TIQMRLRDRK DSPQLLMDAK HIFPVTFPFN PSSLALETIQ IPASLGLGFI

ARV
Length:1,853
Mass (Da):215,538
Last modified:July 27, 2011 - v2
Checksum:iD729DF9222EBCAA0
GO

Computationally mapped potential isoform sequencesi

There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D3Z4J3D3Z4J3_MOUSE
Unconventional myosin-Va
Myo5a
1,855Annotation score:
D3YZ62D3YZ62_MOUSE
Unconventional myosin-Va
Myo5a
1,828Annotation score:
D6RFU0D6RFU0_MOUSE
Unconventional myosin-Va
Myo5a
47Annotation score:
F6TDE5F6TDE5_MOUSE
Unconventional myosin-Va
Myo5a
585Annotation score:
F6Z2S4F6Z2S4_MOUSE
Unconventional myosin-Va
Myo5a
217Annotation score:
B8JK05B8JK05_MOUSE
Unconventional myosin-Va
Myo5a
228Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti695A → R in CAA40651 (PubMed:1996138).Curated1
Sequence conflicti904 – 905EL → DV in CAA40651 (PubMed:1996138).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57377 mRNA Translation: CAA40651.1
AC133947 Genomic DNA No translation available.
CT033761 Genomic DNA No translation available.
CCDSiCCDS52860.1
PIRiA46761
RefSeqiNP_034994.2, NM_010864.2
UniGeneiMm.3645

Genome annotation databases

EnsembliENSMUST00000123128; ENSMUSP00000116028; ENSMUSG00000034593
GeneIDi17918
KEGGimmu:17918
UCSCiuc009qrr.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57377 mRNA Translation: CAA40651.1
AC133947 Genomic DNA No translation available.
CT033761 Genomic DNA No translation available.
CCDSiCCDS52860.1
PIRiA46761
RefSeqiNP_034994.2, NM_010864.2
UniGeneiMm.3645

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2IX7X-ray2.50C763-820[»]
3WB8X-ray2.50A/B/C/D/E/F/G/H1469-1853[»]
4KP3X-ray2.40A/B1469-1853[»]
4ZLKX-ray2.50A1-791[»]
ProteinModelPortaliQ99104
SMRiQ99104
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201666, 22 interactors
CORUMiQ99104
DIPiDIP-29542N
IntActiQ99104, 21 interactors
MINTiQ99104
STRINGi10090.ENSMUSP00000116028

PTM databases

iPTMnetiQ99104
PhosphoSitePlusiQ99104
SwissPalmiQ99104

Proteomic databases

EPDiQ99104
MaxQBiQ99104
PaxDbiQ99104
PeptideAtlasiQ99104
PRIDEiQ99104

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000123128; ENSMUSP00000116028; ENSMUSG00000034593
GeneIDi17918
KEGGimmu:17918
UCSCiuc009qrr.1 mouse

Organism-specific databases

CTDi4644
MGIiMGI:105976 Myo5a

Phylogenomic databases

eggNOGiKOG0160 Eukaryota
KOG0161 Eukaryota
COG5022 LUCA
GeneTreeiENSGT00900000140810
HOGENOMiHOG000171839
HOVERGENiHBG052556
InParanoidiQ99104
KOiK10357
TreeFamiTF328771

Enzyme and pathway databases

ReactomeiR-MMU-2029482 Regulation of actin dynamics for phagocytic cup formation

Miscellaneous databases

ChiTaRSiMyo5a mouse
EvolutionaryTraceiQ99104
PROiPR:Q99104
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000034593 Expressed in 270 organ(s), highest expression level in medial geniculate body
CleanExiMM_MYO5A
ExpressionAtlasiQ99104 baseline and differential
GenevisibleiQ99104 MM

Family and domain databases

CDDicd15478 Myo5a_CBD, 1 hit
cd01380 MYSc_Myo5, 1 hit
Gene3Di2.30.30.360, 1 hit
3.40.850.10, 3 hits
InterProiView protein in InterPro
IPR002710 Dilute_dom
IPR000048 IQ_motif_EF-hand-BS
IPR036961 Kinesin_motor_dom_sf
IPR037988 Myo5a_CBD
IPR001609 Myosin_head_motor_dom
IPR004009 Myosin_N
IPR008989 Myosin_S1_N
IPR036103 MYSc_Myo5
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF01843 DIL, 1 hit
PF00612 IQ, 6 hits
PF00063 Myosin_head, 1 hit
PRINTSiPR00193 MYOSINHEAVY
SMARTiView protein in SMART
SM01132 DIL, 1 hit
SM00015 IQ, 6 hits
SM00242 MYSc, 1 hit
SUPFAMiSSF52540 SSF52540, 2 hits
PROSITEiView protein in PROSITE
PS51126 DILUTE, 1 hit
PS50096 IQ, 6 hits
PS51456 MYOSIN_MOTOR, 1 hit
PS51844 SH3_LIKE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMYO5A_MOUSE
AccessioniPrimary (citable) accession number: Q99104
Secondary accession number(s): E9PUE5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: July 27, 2011
Last modified: September 12, 2018
This is version 175 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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