UniProtKB - Q98SM3 (EZH2A_XENLA)
Protein
Histone-lysine N-methyltransferase EZH2
Gene
ezh2-a
Organism
Xenopus laevis (African clawed frog)
Status
Functioni
Polycomb group (PcG) protein (By similarity). Catalytic subunit of the prc2/eed-ezh2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene (By similarity). May repress transcription of the egr2 and en2 genes. May regulate the circadian clock via histone methylation at the promoter of the circadian genes (By similarity).By similarity1 Publication
Catalytic activityi
- L-lysyl27-[histone H3] + 3 S-adenosyl-L-methionine = 3 H+ + N6,N6,N6-trimethyl-L-lysyl27-[histone H3] + 3 S-adenosyl-L-homocysteineBy similarityEC:2.1.1.356By similarity
GO - Molecular functioni
- histone-lysine N-methyltransferase activity Source: InterPro
- promoter-specific chromatin binding Source: UniProtKB
GO - Biological processi
- negative regulation of gene expression, epigenetic Source: UniProtKB
- rhythmic process Source: UniProtKB-KW
Keywordsi
Molecular function | Chromatin regulator, Methyltransferase, Repressor, Transferase |
Biological process | Biological rhythms, Transcription, Transcription regulation |
Ligand | S-adenosyl-L-methionine |
Names & Taxonomyi
Protein namesi | Recommended name: Histone-lysine N-methyltransferase EZH2 (EC:2.1.1.356By similarity)Alternative name(s): Enhancer of zeste homolog 2-A |
Gene namesi | Name:ezh2-a Synonyms:xez |
Organismi | Xenopus laevis (African clawed frog) |
Taxonomic identifieri | 8355 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus |
Organism-specific databases
Xenbasei | XB-GENE-956220, ezh2.L |
Subcellular locationi
Nucleus
- Nucleus By similarity
Nucleus
- ESC/E(Z) complex Source: UniProtKB
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000345428 | 1 – 748 | Histone-lysine N-methyltransferase EZH2Add BLAST | 748 |
Proteomic databases
PRIDEi | Q98SM3 |
Expressioni
Developmental stagei
Does not appear to be maternally expressed. Zygotic expression begins at late blastula, increases at mid and late gastrula, and peaks in the early neurula. Expression is restricted to the anterior nervous system.1 Publication
Inductioni
Induced by inhibition of BMP signaling.1 Publication
Interactioni
Subunit structurei
Component of the prc2/eed-ezh2 complex.
By similarityProtein-protein interaction databases
BioGRIDi | 100499, 6 interactors |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 505 – 607 | CXCPROSITE-ProRule annotationAdd BLAST | 103 | |
Domaini | 614 – 729 | SETPROSITE-ProRule annotationAdd BLAST | 116 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 183 – 211 | Asp-richAdd BLAST | 29 | |
Compositional biasi | 525 – 590 | Cys-richAdd BLAST | 66 |
Sequence similaritiesi
Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. EZ subfamily.PROSITE-ProRule annotation
Phylogenomic databases
OrthoDBi | 875190at2759 |
Family and domain databases
CDDi | cd00167, SANT, 1 hit |
InterProi | View protein in InterPro IPR026489, CXC_dom IPR021654, EZH1/EZH2 IPR041343, PRC2_HTH_1 IPR041355, Pre-SET_CXC IPR001005, SANT/Myb IPR001214, SET_dom IPR033467, Tesmin/TSO1-like_CXC |
Pfami | View protein in Pfam PF11616, EZH2_WD-Binding, 1 hit PF18118, PRC2_HTH_1, 1 hit PF18264, preSET_CXC, 1 hit PF00856, SET, 1 hit |
SMARTi | View protein in SMART SM01114, CXC, 1 hit SM00717, SANT, 2 hits SM00317, SET, 1 hit |
PROSITEi | View protein in PROSITE PS51633, CXC, 1 hit PS50280, SET, 1 hit |
i Sequence
Sequence statusi: Complete.
Q98SM3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGQTGKKSEK GPVCWRKRVK SEYMALRQLK KFRRADEVKS MFNTNRQKIM
60 70 80 90 100
ERTEILNQEW KQRRIQPVHI MTTVSSLRGT RECSVTSDLD FPKQVIPLKT
110 120 130 140 150
LTAVASVPIM YSWSPLQQNF MVEDETVLHN IPYMGDEVLD QDGTFIEELI
160 170 180 190 200
KNYDGKVHGD RECGFINDEI FVELVNALAQ YSDYEDDEDG DDNQDDEQDD
210 220 230 240 250
TAKDQDDNME DKETQPLRKF PSDKIFEAIS SMFPDKGTSE ELKEKYKELT
260 270 280 290 300
EQQLPGALPP ECTPNIDGPN AKSVQREQSL HSFHTLFCRP CFKYDCFLHP
310 320 330 340 350
FHATPNTYKR KNNEAANDGK PCGPHCYQLL EGAREFAAAL TAERIKTPPK
360 370 380 390 400
RPSGRRRGRL PNNTSRPSTP TVNVSEAKDT DSDREAGTET GGESNDKEEE
410 420 430 440 450
EKKDETSSSS EANSRCQTPI KMKPNIEPPE NVEWSGAEAS LFRVLIGTYY
460 470 480 490 500
DNFCAIARLI GTKTCRQVYE FRVKESSIIS PVIAEDVDTP PRKKKRKHRL
510 520 530 540 550
WAAHCRKIQL KKDGSSNHVY NYQPCDHPRQ PCDSSCPCVI AQNFCEKFCQ
560 570 580 590 600
CSSECQNRFP GCRCKAQCNT KQCPCYLAVR ECDPDLCLTC GAADHWDSKN
610 620 630 640 650
VSCKNCSIQR GSKKHLLLAP SDVAGWGIFI NDTVQKNEFI SEYCGEIISQ
660 670 680 690 700
DEADRRGKVY DKYMCSFLFN LNNDFVVDAT RKGNKIRFAN HSVNPNCYAK
710 720 730 740
VMMVNGDHRI GIFAKRAIQT GEELFFDYRY SQADALKYVG IEREMEIP
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 25 | A → R in AAH84193 (Ref. 2) Curated | 1 | |
Sequence conflicti | 31 | K → R in AAH84193 (Ref. 2) Curated | 1 | |
Sequence conflicti | 290 | P → R in AAH84193 (Ref. 2) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF351126 mRNA Translation: AAK30208.1 BC084193 mRNA Translation: AAH84193.1 |
RefSeqi | NP_001083886.1, NM_001090417.1 XP_018121884.1, XM_018266395.1 |
Genome annotation databases
GeneIDi | 399174 |
KEGGi | xla:399174 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF351126 mRNA Translation: AAK30208.1 BC084193 mRNA Translation: AAH84193.1 |
RefSeqi | NP_001083886.1, NM_001090417.1 XP_018121884.1, XM_018266395.1 |
3D structure databases
SMRi | Q98SM3 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 100499, 6 interactors |
Proteomic databases
PRIDEi | Q98SM3 |
Genome annotation databases
GeneIDi | 399174 |
KEGGi | xla:399174 |
Organism-specific databases
CTDi | 399174 |
Xenbasei | XB-GENE-956220, ezh2.L |
Phylogenomic databases
OrthoDBi | 875190at2759 |
Family and domain databases
CDDi | cd00167, SANT, 1 hit |
InterProi | View protein in InterPro IPR026489, CXC_dom IPR021654, EZH1/EZH2 IPR041343, PRC2_HTH_1 IPR041355, Pre-SET_CXC IPR001005, SANT/Myb IPR001214, SET_dom IPR033467, Tesmin/TSO1-like_CXC |
Pfami | View protein in Pfam PF11616, EZH2_WD-Binding, 1 hit PF18118, PRC2_HTH_1, 1 hit PF18264, preSET_CXC, 1 hit PF00856, SET, 1 hit |
SMARTi | View protein in SMART SM01114, CXC, 1 hit SM00717, SANT, 2 hits SM00317, SET, 1 hit |
PROSITEi | View protein in PROSITE PS51633, CXC, 1 hit PS50280, SET, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | EZH2A_XENLA | |
Accessioni | Q98SM3Primary (citable) accession number: Q98SM3 Secondary accession number(s): Q5XH80 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 22, 2008 |
Last sequence update: | June 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 83 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |