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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Mycoplasma pulmonis (strain UAB CTIP)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei551Aminoacyl-adenylateUniRule annotation1
Binding sitei595ATPUniRule annotation1
Metal bindingi862ZincUniRule annotation1
Metal bindingi865ZincUniRule annotation1
Metal bindingi879ZincUniRule annotation1
Metal bindingi882ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciMPUL272635:G1GT6-677-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:MYPU_6670
OrganismiMycoplasma pulmonis (strain UAB CTIP)
Taxonomic identifieri272635 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
Proteomesi
  • UP000000528 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000984251 – 888Isoleucine--tRNA ligaseAdd BLAST888

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi272635.MYPU_6670

Structurei

3D structure databases

ProteinModelPortaliQ98PQ2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi61 – 71"HIGH" regionAdd BLAST11
Motifi592 – 596"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
HOGENOMiHOG000246402
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG091H028I

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
IPR010663 Znf_FPG/IleRS
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PF06827 zf-FPG_IleRS, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q98PQ2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNEKKDYKDT LNMPQTNFEM QAGLTRKEAQ FRQRWLDNKL YHKILAKNKN
60 70 80 90 100
NKQFVVHDGP PYANGSIHIG HALNKILKDI VVRFKSLQGF YSPFVPGWDT
110 120 130 140 150
HGLPIENKML SELKVNHKQI EVVKLRKEAA KYALNQMLIQ KEQFLKLQML
160 170 180 190 200
SDFEEIYLTL DKNFEAKQLK LFKKMFFDGL IYKGLKPVYW SPSSMSALAE
210 220 230 240 250
AEVEYYDHVS PSIYTCFTIT KGNEFVEVDD ELLIWTTTPW TLIANSGVAV
260 270 280 290 300
GLDIEYSKVK FNKKNYIVAS DLLEKVMEIF GVQKYKVVDT FKGKNLLGVE
310 320 330 340 350
YQRPIKTDLF GIVVAGYHVS IDAGTGLVHM APLFGEDDFI IGQENELDQI
360 370 380 390 400
MHINDDGSIN SEGDEFQGLF YSSANIKIKE FLEKNDKVMF FEYFTHSYPH
410 420 430 440 450
DWRTKKPIIF RGTPQWFVSI DKIKPAILKE IEKIEGRPSW AVKRLATMIE
460 470 480 490 500
NRKTWTISRQ RSWGVPIPIF YNEKNEIVND EKVFDHVIEL VEKYGSDVWF
510 520 530 540 550
EKSVDELLPS EYKNKNWTKE TNIMDVWFDS GSTSIGVEIE GVSVPFDLYL
560 570 580 590 600
EGIDQYRGWF NSSIINSVAY WGQSPYRLLL SHGFVLDGKG KKMSKQLGNV
610 620 630 640 650
VDPQEIIQKY GADILRLWVA NCEYAHDVSV SESIIKQTVE NYRKIRNTIK
660 670 680 690 700
FLLGNLQDYD HSKFNLKLEG IHELINERLK KVKFDILQAY NDYDFNDVIK
710 720 730 740 750
TLTNFLTDLS SFYLSISKDS LYADKINSKE RRMIQYNMYN ILEATLVVIA
760 770 780 790 800
PIMPTTAEDA YDNFNKQDKQ ESVHLEKMFE ATIADDKLEK TWKEFFDLKD
810 820 830 840 850
EVYKEIEVEI ANKKIKRTND AHVTINTKSN FLMSLDLKKL LMIGKISFGS
860 870 880
SLRVETFDSH KCPRCWNHIE KTEVVEDLCQ RCYQTINS
Length:888
Mass (Da):103,303
Last modified:October 1, 2001 - v1
Checksum:iCCECB4F79293A390
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL445565 Genomic DNA Translation: CAC13840.1
PIRiC90595
RefSeqiWP_010925468.1, NC_002771.1

Genome annotation databases

EnsemblBacteriaiCAC13840; CAC13840; CAC13840
KEGGimpu:MYPU_6670

Similar proteinsi

Entry informationi

Entry nameiSYI_MYCPU
AccessioniPrimary (citable) accession number: Q98PQ2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: October 1, 2001
Last modified: March 28, 2018
This is version 100 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

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