UniProtKB - Q980M5 (TFE_SACS2)
Protein
Transcription factor E
Gene
tfe
Organism
Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus)
Status
Functioni
Transcription factor that plays a role in the activation of archaeal genes transcribed by RNA polymerase. Facilitates transcription initiation by enhancing TATA-box recognition by TATA-box-binding protein (Tbp), and transcription factor B (Tfb) and RNA polymerase recruitment. Not absolutely required for transcription in vitro, but particularly important in cases where Tbp or Tfb function is not optimal. It dynamically alters the nucleic acid-binding properties of RNA polymerases by stabilizing the initiation complex and destabilizing elongation complexes. Seems to translocate with the RNA polymerase following initiation and acts by binding to the non template strand of the transcription bubble in elongation complexes.1 Publication
GO - Molecular functioni
- DNA binding Source: UniProtKB-KW
GO - Biological processi
- regulation of transcription, DNA-templated Source: InterPro
- transcription open complex formation at RNA polymerase II promoter Source: GO_Central
Keywordsi
Molecular function | DNA-binding |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
BioCyci | SSOL273057:G1FZF-275-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Transcription factor EShort name: TFE Alternative name(s): TFIIE subunit alpha homolog Transcription initiation factor TFIIE |
Gene namesi | Name:tfe Ordered Locus Names:SSO0266 |
Organismi | Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus) |
Taxonomic identifieri | 273057 [NCBI] |
Taxonomic lineagei | Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Saccharolobus › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000318948 | 1 – 178 | Transcription factor EAdd BLAST | 178 |
Interactioni
Subunit structurei
Monomer (Probable). Interaction with RNA polymerase subunits RpoF and RpoE is necessary for Tfe stimulatory transcription activity. Able to interact with Tbp and RNA polymerase in the absence of DNA promoter.
Interacts both with the preinitiation and elongation complexes (By similarity).
By similarityCuratedProtein-protein interaction databases
STRINGi | 273057.SSO0266 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q980M5 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q980M5 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 4 – 88 | HTH TFE/IIEalpha-typeAdd BLAST | 85 |
Domaini
The winged helix domain is involved in binding to DNA in the preinitiation complex.By similarity
Sequence similaritiesi
Belongs to the TFE family.Curated
Phylogenomic databases
eggNOGi | arCOG04270, Archaea |
HOGENOMi | CLU_100097_0_0_2 |
InParanoidi | Q980M5 |
OMAi | DSGWLTY |
Family and domain databases
Gene3Di | 1.10.10.10, 1 hit |
HAMAPi | MF_01909, TFE_arch, 1 hit |
InterProi | View protein in InterPro IPR016481, TF_E_archaea IPR039997, TFE IPR017919, TFIIE/TFIIEa_HTH IPR002853, TFIIE_asu IPR024550, TFIIEa/SarR/Rpc3_HTH_dom IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
PANTHERi | PTHR13097, PTHR13097, 1 hit |
Pfami | View protein in Pfam PF02002, TFIIE_alpha, 1 hit |
PIRSFi | PIRSF006373, TF_E_archaea, 1 hit |
SMARTi | View protein in SMART SM00531, TFIIE, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit |
PROSITEi | View protein in PROSITE PS51344, HTH_TFE_IIE, 1 hit |
i Sequence
Sequence statusi: Complete.
Q980M5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVNAEDLFIN LAKSLLGDDV IDVLRILLDK GTEMTDEEIA NQLNIKVNDV
60 70 80 90 100
RKKLNLLEEQ GFVSYRKTRD KDSGWFIYYW KPNIDQINEI LLNRKRLILD
110 120 130 140 150
KLKTRLEYEK NNTFFICPQD NSRYSFEEAF ENEFKCLKCG SQLTYYDTDK
160 170
IKSFLEQKIR QIEEEIDKET KLGANKNH
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE006641 Genomic DNA Translation: AAK40605.1 |
PIRi | F90168 |
RefSeqi | WP_009990539.1, NC_002754.1 |
Genome annotation databases
EnsemblBacteriai | AAK40605; AAK40605; SSO0266 |
GeneIDi | 44129239 |
KEGGi | sso:SSO0266 |
PATRICi | fig|273057.12.peg.261 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE006641 Genomic DNA Translation: AAK40605.1 |
PIRi | F90168 |
RefSeqi | WP_009990539.1, NC_002754.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1Q1H | X-ray | 2.90 | A | 1-110 | [»] | |
SMRi | Q980M5 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 273057.SSO0266 |
Genome annotation databases
EnsemblBacteriai | AAK40605; AAK40605; SSO0266 |
GeneIDi | 44129239 |
KEGGi | sso:SSO0266 |
PATRICi | fig|273057.12.peg.261 |
Phylogenomic databases
eggNOGi | arCOG04270, Archaea |
HOGENOMi | CLU_100097_0_0_2 |
InParanoidi | Q980M5 |
OMAi | DSGWLTY |
Enzyme and pathway databases
BioCyci | SSOL273057:G1FZF-275-MONOMER |
Miscellaneous databases
EvolutionaryTracei | Q980M5 |
Family and domain databases
Gene3Di | 1.10.10.10, 1 hit |
HAMAPi | MF_01909, TFE_arch, 1 hit |
InterProi | View protein in InterPro IPR016481, TF_E_archaea IPR039997, TFE IPR017919, TFIIE/TFIIEa_HTH IPR002853, TFIIE_asu IPR024550, TFIIEa/SarR/Rpc3_HTH_dom IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
PANTHERi | PTHR13097, PTHR13097, 1 hit |
Pfami | View protein in Pfam PF02002, TFIIE_alpha, 1 hit |
PIRSFi | PIRSF006373, TF_E_archaea, 1 hit |
SMARTi | View protein in SMART SM00531, TFIIE, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit |
PROSITEi | View protein in PROSITE PS51344, HTH_TFE_IIE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TFE_SACS2 | |
Accessioni | Q980M5Primary (citable) accession number: Q980M5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 26, 2008 |
Last sequence update: | October 1, 2001 | |
Last modified: | April 7, 2021 | |
This is version 119 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families