UniProtKB - Q97Z83 (PRIS_SACS2)
Protein
DNA primase small subunit PriS
Gene
priS
Organism
Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus)
Status
Functioni
Catalytic subunit of DNA primase, an RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication. The small subunit contains the primase catalytic core and has DNA synthesis activity on its own. Binding to the large subunit stabilizes and modulates the activity, increasing the rate of DNA synthesis while decreasing the length of the DNA fragments, and conferring RNA synthesis capability. The DNA polymerase activity may enable DNA primase to also catalyze primer extension after primer synthesis. May also play a role in DNA repair. Possesses a template-independent 3'-terminal nucleotidyl transferase activity.UniRule annotation1 Publication
Miscellaneous
The bound zinc ion is not a cofactor. It is bound to a zinc knuckle motif that may be involved in sequence recognition and the binding of ssDNA (PubMed:16273105).1 Publication
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 101 | UniRule annotation | 1 | |
Active sitei | 103 | UniRule annotation | 1 | |
Metal bindingi | 116 | Zinc1 Publication | 1 | |
Metal bindingi | 119 | Zinc1 Publication | 1 | |
Metal bindingi | 128 | Zinc1 Publication | 1 | |
Metal bindingi | 131 | Zinc1 Publication | 1 | |
Active sitei | 235 | UniRule annotation | 1 |
GO - Molecular functioni
- DNA primase activity Source: GO_Central
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- DNA replication, synthesis of RNA primer Source: GO_Central
Keywordsi
Molecular function | Nucleotidyltransferase, Transferase |
Biological process | DNA replication, Transcription |
Ligand | Magnesium, Manganese, Metal-binding, Zinc |
Enzyme and pathway databases
BioCyci | SSOL273057:G1FZF-1079-MONOMER |
BRENDAi | 2.7.7.B16, 6163 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:priSUniRule annotation Synonyms:priA Ordered Locus Names:SSO1048 |
Organismi | Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus) |
Taxonomic identifieri | 273057 [NCBI] |
Taxonomic lineagei | Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Saccharolobus › |
Proteomesi |
|
Subcellular locationi
Other locations
- primosome complex Source: UniProtKB-KW
Keywords - Cellular componenti
DNA-directed RNA polymerase, PrimosomePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 164 | F → E: Strong decrease in interaction with PriL. 1 Publication | 1 | |
Mutagenesisi | 165 | G → I: Partial destabilization of PriS-PriL complex. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000046754 | 1 – 330 | DNA primase small subunit PriSAdd BLAST | 330 |
Interactioni
Subunit structurei
Heterodimer of a small subunit (PriS) and a large subunit (PriL).
UniRule annotation2 PublicationsBinary interactionsi
Q97Z83
With | #Exp. | IntAct |
---|---|---|
priL [Q9UWW1] | 5 | EBI-8081502,EBI-8081454 |
Protein-protein interaction databases
DIPi | DIP-42889N |
IntActi | Q97Z83, 2 interactors |
MINTi | Q97Z83 |
STRINGi | 273057.SSO1048 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q97Z83 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q97Z83 |
Family & Domainsi
Sequence similaritiesi
Belongs to the eukaryotic-type primase small subunit family.UniRule annotation
Phylogenomic databases
eggNOGi | arCOG04110, Archaea |
HOGENOMi | CLU_056123_0_0_2 |
InParanoidi | Q97Z83 |
OMAi | GYHVHVR |
Family and domain databases
CDDi | cd04860, AE_Prim_S, 1 hit |
HAMAPi | MF_00700, DNA_primase_sml_arc, 1 hit |
InterProi | View protein in InterPro IPR014052, DNA_primase_ssu_euk/arc IPR023639, DNA_primase_ssu_PriS |
PANTHERi | PTHR10536, PTHR10536, 1 hit |
i Sequence
Sequence statusi: Complete.
Q97Z83-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGTFTLHQGQ TNLIKSFFRN YYLNAELELP KDMELREFAL QPFGSDTYVR
60 70 80 90 100
HLSFSSSEEL RDYLVNRNLP LHLFYSSARY QLPSARNMEE KAWMGSDLLF
110 120 130 140 150
DIDADHLCKL RSIRFCPVCG NAVVSEKCER DNVETLEYVE MTSECIKRGL
160 170 180 190 200
EQTRNLVEIL EDDFGLKPKV YFSGNRGFHV QVDCYGNCAL LDSDERKEIA
210 220 230 240 250
EYVMGIGVPG YPGGSENAPG WVGRKNRGIN GVTIDEQVTI DVKRLIRIPN
260 270 280 290 300
SLHGKSGLIV KRVPNLDDFE FNETLSPFTG YTIFLPYITI ETEVLGSIIK
310 320 330
LNRGIPIKIK SSIGIYLHLR NLGEVKAYVR
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE006641 Genomic DNA Translation: AAK41310.1 |
PIRi | G90256 |
RefSeqi | WP_009989180.1, NC_002754.1 |
Genome annotation databases
EnsemblBacteriai | AAK41310; AAK41310; SSO1048 |
GeneIDi | 44129977 |
KEGGi | sso:SSO1048 |
PATRICi | fig|273057.12.peg.1042 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE006641 Genomic DNA Translation: AAK41310.1 |
PIRi | G90256 |
RefSeqi | WP_009989180.1, NC_002754.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1ZT2 | X-ray | 3.33 | A/C | 1-330 | [»] | |
5OF3 | X-ray | 2.91 | A/D | 1-330 | [»] | |
5OFN | X-ray | 3.00 | A | 1-330 | [»] | |
SMRi | Q97Z83 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
DIPi | DIP-42889N |
IntActi | Q97Z83, 2 interactors |
MINTi | Q97Z83 |
STRINGi | 273057.SSO1048 |
Genome annotation databases
EnsemblBacteriai | AAK41310; AAK41310; SSO1048 |
GeneIDi | 44129977 |
KEGGi | sso:SSO1048 |
PATRICi | fig|273057.12.peg.1042 |
Phylogenomic databases
eggNOGi | arCOG04110, Archaea |
HOGENOMi | CLU_056123_0_0_2 |
InParanoidi | Q97Z83 |
OMAi | GYHVHVR |
Enzyme and pathway databases
BioCyci | SSOL273057:G1FZF-1079-MONOMER |
BRENDAi | 2.7.7.B16, 6163 |
Miscellaneous databases
EvolutionaryTracei | Q97Z83 |
Family and domain databases
CDDi | cd04860, AE_Prim_S, 1 hit |
HAMAPi | MF_00700, DNA_primase_sml_arc, 1 hit |
InterProi | View protein in InterPro IPR014052, DNA_primase_ssu_euk/arc IPR023639, DNA_primase_ssu_PriS |
PANTHERi | PTHR10536, PTHR10536, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PRIS_SACS2 | |
Accessioni | Q97Z83Primary (citable) accession number: Q97Z83 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 29, 2003 |
Last sequence update: | October 1, 2001 | |
Last modified: | April 7, 2021 | |
This is version 105 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families