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UniProtKB - Q97W92 (HEXPP_SACS2)
Protein
Hexaprenyl pyrophosphate synthase
Gene
gdS-2
Organism
Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus)
Status
Functioni
Catalyzes consecutive E-type condensation of two isopentenyl pyrophosphate (IPP) molecules with an allylic substrate such as geranylgeranyl diphosphate (GGPP), farnesyl diphosphate (FPP) or geranyl diphosphate (GPP) to yield the medium-chain product trans-C30-hexaprenyl pyrophosphate (HexPP). GGPP is the physiological substrate.
2 PublicationsCatalytic activityi
- (2E,6E,10E)-geranylgeranyl diphosphate + 2 isopentenyl diphosphate = all-trans-hexaprenyl diphosphate + 2 diphosphate2 PublicationsEC:2.5.1.822 Publications
Cofactori
Mg2+1 PublicationNote: Binds 2 Mg2+ ions per subunit.By similarity
pH dependencei
Optimum pH is 6.1 Publication
Temperature dependencei
Highly thermostable, remains fully active after 2 hours at 80 degrees Celsius.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 42 | Isopentenyl diphosphateBy similarity | 1 | |
Binding sitei | 45 | Isopentenyl diphosphateBy similarity | 1 | |
Binding sitei | 74 | Isopentenyl diphosphateBy similarity | 1 | |
Metal bindingi | 81 | Magnesium 1By similarity | 1 | |
Metal bindingi | 81 | Magnesium 2By similarity | 1 | |
Metal bindingi | 85 | Magnesium 1By similarity | 1 | |
Metal bindingi | 85 | Magnesium 2By similarity | 1 | |
Binding sitei | 91 | Isopentenyl diphosphateBy similarity | 1 |
GO - Molecular functioni
- hexaprenyl diphosphate synthase (geranylgeranyl-diphosphate specific) activity Source: UniProtKB
- metal ion binding Source: UniProtKB-KW
- prenyltransferase activity Source: GO_Central
GO - Biological processi
- isoprenoid biosynthetic process Source: UniProtKB
Keywordsi
Molecular function | Transferase |
Biological process | Isoprene biosynthesis |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
BRENDAi | 2.5.1.82, 6163 |
Names & Taxonomyi
Protein namesi | Recommended name: Hexaprenyl pyrophosphate synthase (EC:2.5.1.822 Publications)Short name: HexPP Alternative name(s): Medium-chain HexPP |
Gene namesi | Name:gdS-2 Ordered Locus Names:SSO2345 |
Organismi | Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus) |
Taxonomic identifieri | 273057 [NCBI] |
Taxonomic lineagei | Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Saccharolobus › |
Proteomesi |
|
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 124 | Y → A: Generates the same C30 product as the wild-type. 1 Publication | 1 | |
Mutagenesisi | 164 | L → A: Generates only one C5-prenyl unit longer C35 product. 1 Publication | 1 | |
Mutagenesisi | 174 | Y → A: Generates the same C30 product as the wild-type. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000418599 | 1 – 281 | Hexaprenyl pyrophosphate synthaseAdd BLAST | 281 |
Proteomic databases
PRIDEi | Q97W92 |
Interactioni
Subunit structurei
Homodimer.
1 Publication1 PublicationProtein-protein interaction databases
STRINGi | 273057.SSO2345 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | Q97W92 |
SMRi | Q97W92 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q97W92 |
Family & Domainsi
Sequence similaritiesi
Belongs to the FPP/GGPP synthase family.Curated
Phylogenomic databases
eggNOGi | arCOG01727, Archaea |
HOGENOMi | CLU_014015_5_0_2 |
InParanoidi | Q97W92 |
OMAi | WLIAESI |
PhylomeDBi | Q97W92 |
Family and domain databases
Gene3Di | 1.10.600.10, 1 hit |
InterProi | View protein in InterPro IPR008949, Isoprenoid_synthase_dom_sf IPR000092, Polyprenyl_synt IPR033749, Polyprenyl_synt_CS |
Pfami | View protein in Pfam PF00348, polyprenyl_synt, 1 hit |
SUPFAMi | SSF48576, SSF48576, 1 hit |
PROSITEi | View protein in PROSITE PS00444, POLYPRENYL_SYNTHASE_2, 1 hit |
i Sequence
Sequence statusi: Complete.
Q97W92-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSIIEFWLEA KATIDRLIEQ FLNSNRDWDL VDISSYILKD GKRFRGTLNM
60 70 80 90 100
FFTVALGGDI KDSYGGALAI EILHSASLAL DDIVDLDATR RGDKAAWVVY
110 120 130 140 150
GNRKVIFITN YLIPTALRII QTSYGDDALN TSIELWKDTS VGALRDMYDN
160 170 180 190 200
SDYIRTIELK TGSLFKLSTV LSAYASKHYN TKQQMLDVGK YLGIIYQVID
210 220 230 240 250
DFVDYKTKKV EEIDGSAKQL FKYYREGKLE EYVRSVYLEY KQKYDELISN
260 270 280
IPFQSKYLSE IRSLPEFLAN GLLKEANIDK I
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE006641 Genomic DNA Translation: AAK42496.1 |
PIRi | A99405 |
RefSeqi | WP_009989514.1, NC_002754.1 |
Genome annotation databases
EnsemblBacteriai | AAK42496; AAK42496; SSO2345 |
GeneIDi | 44128070 |
KEGGi | sso:SSO2345 |
PATRICi | fig|273057.12.peg.2429 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE006641 Genomic DNA Translation: AAK42496.1 |
PIRi | A99405 |
RefSeqi | WP_009989514.1, NC_002754.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2AZJ | X-ray | 2.40 | A/B | 1-281 | [»] | |
2AZK | X-ray | 2.70 | A/B | 1-281 | [»] | |
AlphaFoldDBi | Q97W92 | |||||
SMRi | Q97W92 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 273057.SSO2345 |
Proteomic databases
PRIDEi | Q97W92 |
Genome annotation databases
EnsemblBacteriai | AAK42496; AAK42496; SSO2345 |
GeneIDi | 44128070 |
KEGGi | sso:SSO2345 |
PATRICi | fig|273057.12.peg.2429 |
Phylogenomic databases
eggNOGi | arCOG01727, Archaea |
HOGENOMi | CLU_014015_5_0_2 |
InParanoidi | Q97W92 |
OMAi | WLIAESI |
PhylomeDBi | Q97W92 |
Enzyme and pathway databases
BRENDAi | 2.5.1.82, 6163 |
Miscellaneous databases
EvolutionaryTracei | Q97W92 |
Family and domain databases
Gene3Di | 1.10.600.10, 1 hit |
InterProi | View protein in InterPro IPR008949, Isoprenoid_synthase_dom_sf IPR000092, Polyprenyl_synt IPR033749, Polyprenyl_synt_CS |
Pfami | View protein in Pfam PF00348, polyprenyl_synt, 1 hit |
SUPFAMi | SSF48576, SSF48576, 1 hit |
PROSITEi | View protein in PROSITE PS00444, POLYPRENYL_SYNTHASE_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | HEXPP_SACS2 | |
Accessioni | Q97W92Primary (citable) accession number: Q97W92 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 5, 2012 |
Last sequence update: | October 1, 2001 | |
Last modified: | May 25, 2022 | |
This is version 114 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families