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UniProtKB - Q97W02 (DPO4_SACS2)
Protein
DNA polymerase IV
Gene
dbh
Organism
Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus)
Status
Functioni
Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity. It is involved in translesional synthesis.
Catalytic activityi
- EC:2.7.7.7
Cofactori
Mg2+Note: Binds 2 magnesium ions per subunit.
Temperature dependencei
Highly active from 35 to 95 degrees Celsius. Thermostable.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 7 | Magnesium | 1 | |
Sitei | 12 | Substrate discrimination | 1 | |
Metal bindingi | 105 | Magnesium | 1 | |
Active sitei | 106 | 1 |
GO - Molecular functioni
- damaged DNA binding Source: InterPro
- DNA-directed DNA polymerase activity Source: GO_Central
- magnesium ion binding Source: UniProtKB-UniRule
GO - Biological processi
- DNA-dependent DNA replication Source: UniProtKB-UniRule
- error-prone translesion synthesis Source: GO_Central
Keywordsi
Molecular function | DNA-binding, DNA-directed DNA polymerase, Mutator protein, Nucleotidyltransferase, Transferase |
Biological process | DNA damage, DNA repair, DNA replication |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
BRENDAi | 2.7.7.7, 6163 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:dbh Synonyms:dpo4 Ordered Locus Names:SSO2448 |
Organismi | Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus) |
Taxonomic identifieri | 273057 [NCBI] |
Taxonomic lineagei | Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Saccharolobus › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm Curated
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 105 – 106 | DE → AA: Loss of function. 1 Publication | 2 | |
Mutagenesisi | 342 – 352 | Missing : Almost complete loss of interaction with PCNA. 1 PublicationAdd BLAST | 11 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000173976 | 1 – 352 | DNA polymerase IVAdd BLAST | 352 |
Interactioni
Subunit structurei
Monomer.
Interacts with the PCNA heterotrimer via PCNA1.
1 PublicationProtein-protein interaction databases
DIPi | DIP-48855N |
IntActi | Q97W02, 1 interactor |
STRINGi | 273057.SSO2448 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | Q97W02 |
SMRi | Q97W02 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q97W02 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 3 – 187 | UmuCAdd BLAST | 185 |
Domaini
The catalytic core consists of fingers, palm and thumb subdomains, but the fingers and thumb subdomains are much smaller than in high-fidelity polymerases; residues from five sequence motifs of the Y-family cluster around an active site cleft that can accommodate DNA and nucleotide substrates with relaxed geometric constraints, with consequently higher rates of misincorporation and low processivity.
Sequence similaritiesi
Belongs to the DNA polymerase type-Y family.Curated
Phylogenomic databases
eggNOGi | arCOG04582, Archaea |
HOGENOMi | CLU_012348_1_2_2 |
InParanoidi | Q97W02 |
OMAi | CKPDGLL |
PhylomeDBi | Q97W02 |
Family and domain databases
CDDi | cd03586, PolY_Pol_IV_kappa, 1 hit |
Gene3Di | 3.30.1490.100, 1 hit 3.30.70.270, 1 hit |
HAMAPi | MF_01113, DNApol_IV, 1 hit |
InterProi | View protein in InterPro IPR043502, DNA/RNA_pol_sf IPR036775, DNA_pol_Y-fam_lit_finger_sf IPR017961, DNA_pol_Y-fam_little_finger IPR022880, DNApol_IV IPR024728, PolY_HhH_motif IPR043128, Rev_trsase/Diguanyl_cyclase IPR001126, UmuC |
Pfami | View protein in Pfam PF00817, IMS, 1 hit PF11799, IMS_C, 1 hit PF11798, IMS_HHH, 1 hit |
SUPFAMi | SSF100879, SSF100879, 1 hit SSF56672, SSF56672, 1 hit |
PROSITEi | View protein in PROSITE PS50173, UMUC, 1 hit |
i Sequence
Sequence statusi: Complete.
Q97W02-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MIVLFVDFDY FYAQVEEVLN PSLKGKPVVV CVFSGRFEDS GAVATANYEA
60 70 80 90 100
RKFGVKAGIP IVEAKKILPN AVYLPMRKEV YQQVSSRIMN LLREYSEKIE
110 120 130 140 150
IASIDEAYLD ISDKVRDYRE AYNLGLEIKN KILEKEKITV TVGISKNKVF
160 170 180 190 200
AKIAADMAKP NGIKVIDDEE VKRLIRELDI ADVPGIGNIT AEKLKKLGIN
210 220 230 240 250
KLVDTLSIEF DKLKGMIGEA KAKYLISLAR DEYNEPIRTR VRKSIGRIVT
260 270 280 290 300
MKRNSRNLEE IKPYLFRAIE ESYYKLDKRI PKAIHVVAVT EDLDIVSRGR
310 320 330 340 350
TFPHGISKET AYSESVKLLQ KILEEDERKI RRIGVRFSKF IEAIGLDKFF
DT
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE006641 Genomic DNA Translation: AAK42588.1 |
PIRi | E90416 |
RefSeqi | WP_009993137.1, NC_002754.1 |
Genome annotation databases
EnsemblBacteriai | AAK42588; AAK42588; SSO2448 |
GeneIDi | 44128166 |
KEGGi | sso:SSO2448 |
PATRICi | fig|273057.12.peg.2526 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE006641 Genomic DNA Translation: AAK42588.1 |
PIRi | E90416 |
RefSeqi | WP_009993137.1, NC_002754.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1JX4 | X-ray | 1.70 | A | 1-352 | [»] | |
1JXL | X-ray | 2.10 | A | 1-352 | [»] | |
1N48 | X-ray | 2.20 | A | 1-352 | [»] | |
1N56 | X-ray | 2.40 | A/B | 1-352 | [»] | |
1RYR | X-ray | 2.28 | A | 1-352 | [»] | |
1RYS | X-ray | 2.03 | A/B | 1-352 | [»] | |
1S0M | X-ray | 2.70 | A/B | 1-352 | [»] | |
1S0N | X-ray | 2.80 | A | 1-352 | [»] | |
1S0O | X-ray | 2.10 | A/B | 1-352 | [»] | |
1S10 | X-ray | 2.10 | A | 1-352 | [»] | |
1S97 | X-ray | 2.40 | A/B/C/D | 1-352 | [»] | |
1S9F | X-ray | 2.00 | A/B/C/D | 1-352 | [»] | |
2AGO | X-ray | 2.85 | A | 1-341 | [»] | |
2AGP | X-ray | 2.90 | A/B | 1-341 | [»] | |
2AGQ | X-ray | 2.10 | A | 1-341 | [»] | |
2ASD | X-ray | 1.95 | A/B | 2-352 | [»] | |
2ASJ | X-ray | 2.35 | A/B | 2-352 | [»] | |
2ASL | X-ray | 2.65 | A/B | 2-352 | [»] | |
2ATL | X-ray | 2.80 | A/B | 2-352 | [»] | |
2AU0 | X-ray | 2.70 | A/B | 2-352 | [»] | |
2BQ3 | X-ray | 2.00 | A | 1-352 | [»] | |
2BQR | X-ray | 2.37 | A | 1-352 | [»] | |
2BQU | X-ray | 2.50 | A | 1-352 | [»] | |
2BR0 | X-ray | 2.17 | A | 1-352 | [»] | |
2C22 | X-ray | 2.56 | A | 1-352 | [»] | |
2C28 | X-ray | 2.27 | A | 1-352 | [»] | |
2C2D | X-ray | 2.57 | A | 1-352 | [»] | |
2C2E | X-ray | 2.61 | A | 1-352 | [»] | |
2C2R | X-ray | 2.55 | A | 1-352 | [»] | |
2IA6 | X-ray | 2.50 | A/B | 1-352 | [»] | |
2IBK | X-ray | 2.25 | A | 1-352 | [»] | |
2IMW | X-ray | 2.05 | P | 1-348 | [»] | |
2J6S | X-ray | 2.50 | A | 1-352 | [»] | |
2J6T | X-ray | 2.60 | A | 1-352 | [»] | |
2J6U | X-ray | 2.50 | A | 1-352 | [»] | |
2JEF | X-ray | 2.17 | A | 1-352 | [»] | |
2JEG | X-ray | 2.38 | A | 1-352 | [»] | |
2JEI | X-ray | 2.39 | A | 1-352 | [»] | |
2JEJ | X-ray | 1.86 | A | 1-352 | [»] | |
2R8G | X-ray | 2.70 | A | 1-352 | [»] | |
2R8H | X-ray | 2.48 | A | 1-352 | [»] | |
2R8I | X-ray | 2.38 | A | 1-352 | [»] | |
2RDI | X-ray | 1.92 | A | 1-341 | [»] | |
2RDJ | X-ray | 2.20 | A/B | 1-352 | [»] | |
2UVR | X-ray | 2.90 | A | 1-352 | [»] | |
2UVU | X-ray | 2.70 | A | 1-352 | [»] | |
2UVV | X-ray | 2.20 | A | 1-352 | [»] | |
2UVW | X-ray | 2.09 | A | 1-352 | [»] | |
2V4Q | X-ray | 2.60 | A | 1-352 | [»] | |
2V4R | X-ray | 2.50 | A | 1-352 | [»] | |
2V9W | X-ray | 3.00 | A/B | 1-352 | [»] | |
2VA2 | X-ray | 2.80 | A/B | 1-352 | [»] | |
2VA3 | X-ray | 2.98 | A | 1-352 | [»] | |
2W8K | X-ray | 3.10 | A | 1-352 | [»] | |
2W8L | X-ray | 3.00 | A | 1-352 | [»] | |
2W9A | X-ray | 2.60 | A | 1-352 | [»] | |
2W9B | X-ray | 2.28 | A/B | 1-352 | [»] | |
2W9C | X-ray | 2.87 | A/B | 1-352 | [»] | |
2XC9 | X-ray | 2.20 | A | 1-352 | [»] | |
2XCA | X-ray | 2.50 | A | 1-352 | [»] | |
2XCP | X-ray | 2.60 | A/B | 1-352 | [»] | |
3FDS | X-ray | 2.05 | A | 1-352 | [»] | |
3GII | X-ray | 2.60 | A | 2-341 | [»] | |
3GIJ | X-ray | 2.40 | A/B | 2-341 | [»] | |
3GIK | X-ray | 2.90 | A | 2-341 | [»] | |
3GIL | X-ray | 2.71 | A/B | 2-341 | [»] | |
3GIM | X-ray | 2.70 | A | 2-341 | [»] | |
3KHG | X-ray | 2.96 | A/B | 2-341 | [»] | |
3KHH | X-ray | 2.70 | A/B | 2-341 | [»] | |
3KHL | X-ray | 2.10 | A/B | 2-341 | [»] | |
3KHR | X-ray | 2.01 | A/B | 2-341 | [»] | |
3M9M | X-ray | 2.90 | B | 1-352 | [»] | |
3M9N | X-ray | 1.93 | B | 1-352 | [»] | |
3M9O | X-ray | 2.00 | B | 1-352 | [»] | |
3PR4 | X-ray | 2.65 | A | 1-341 | [»] | |
3PR5 | X-ray | 2.40 | B | 1-341 | [»] | |
3PVX | X-ray | 3.03 | A | 1-341 | [»] | |
3PW0 | X-ray | 2.91 | A | 1-341 | [»] | |
3PW2 | X-ray | 2.74 | A | 1-341 | [»] | |
3PW4 | X-ray | 2.90 | A | 1-341 | [»] | |
3PW5 | X-ray | 3.00 | A | 1-341 | [»] | |
3PW7 | X-ray | 2.90 | A/E | 1-341 | [»] | |
3QZ7 | X-ray | 2.00 | A | 1-352 | [»] | |
3QZ8 | X-ray | 2.00 | A | 1-352 | [»] | |
3RAQ | X-ray | 2.25 | A/B | 2-341 | [»] | |
3RAX | X-ray | 1.89 | A/B | 2-341 | [»] | |
3RB0 | X-ray | 3.22 | A/B | 2-341 | [»] | |
3RB3 | X-ray | 2.80 | A | 2-341 | [»] | |
3RB4 | X-ray | 2.80 | A/B | 2-341 | [»] | |
3RB6 | X-ray | 2.70 | A/B | 2-341 | [»] | |
3RBD | X-ray | 2.50 | A/B | 2-341 | [»] | |
3RBE | X-ray | 2.80 | A/B | 2-341 | [»] | |
3T5H | X-ray | 2.35 | A | 1-341 | [»] | |
3T5J | X-ray | 2.40 | A | 1-341 | [»] | |
3T5K | X-ray | 2.90 | A | 1-341 | [»] | |
3T5L | X-ray | 2.90 | A | 1-341 | [»] | |
3V6H | X-ray | 2.30 | A/B | 1-342 | [»] | |
3V6J | X-ray | 2.30 | A/J | 1-342 | [»] | |
3V6K | X-ray | 3.60 | A/J | 1-342 | [»] | |
4F4W | X-ray | 1.90 | A/B | 231-352 | [»] | |
4F4X | X-ray | 2.05 | A | 231-352 | [»] | |
4F4Y | X-ray | 2.34 | A/B | 5-143 | [»] | |
A/B | 231-247 | [»] | ||||
4F4Z | X-ray | 2.30 | A/B | 1-246 | [»] | |
4F50 | X-ray | 2.22 | A | 246-352 | [»] | |
4FBT | X-ray | 2.00 | A | 1-341 | [»] | |
4FBU | X-ray | 2.60 | A/B | 1-341 | [»] | |
4G3I | X-ray | 2.50 | A/B | 1-341 | [»] | |
4GC6 | X-ray | 2.90 | A | 1-352 | [»] | |
4GC7 | X-ray | 2.89 | A/B | 1-352 | [»] | |
4JUZ | X-ray | 2.65 | A | 1-341 | [»] | |
4JV0 | X-ray | 2.95 | A | 1-341 | [»] | |
4JV1 | X-ray | 2.30 | A | 1-341 | [»] | |
4JV2 | X-ray | 2.74 | A | 1-341 | [»] | |
4QW8 | X-ray | 2.29 | A | 1-341 | [»] | |
4QW9 | X-ray | 2.40 | A | 1-341 | [»] | |
4QWA | X-ray | 2.20 | A | 1-341 | [»] | |
4QWB | X-ray | 1.80 | A | 1-343 | [»] | |
4QWC | X-ray | 2.40 | A/D | 1-342 | [»] | |
4QWD | X-ray | 2.05 | A | 1-341 | [»] | |
4QWE | X-ray | 2.20 | A | 1-341 | [»] | |
4RUA | X-ray | 3.07 | A | 1-341 | [»] | |
4RUC | X-ray | 2.90 | A | 1-341 | [»] | |
4RZR | X-ray | 2.20 | A/D | 1-352 | [»] | |
4TQR | X-ray | 1.58 | A | 1-342 | [»] | |
4TQS | X-ray | 2.06 | A/B | 1-352 | [»] | |
5EDW | X-ray | 2.62 | A | 1-341 | [»] | |
6L84 | X-ray | 2.60 | A | 1-352 | [»] | |
6L97 | X-ray | 2.36 | A/B | 1-352 | [»] | |
BMRBi | Q97W02 | |||||
SMRi | Q97W02 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
DIPi | DIP-48855N |
IntActi | Q97W02, 1 interactor |
STRINGi | 273057.SSO2448 |
Genome annotation databases
EnsemblBacteriai | AAK42588; AAK42588; SSO2448 |
GeneIDi | 44128166 |
KEGGi | sso:SSO2448 |
PATRICi | fig|273057.12.peg.2526 |
Phylogenomic databases
eggNOGi | arCOG04582, Archaea |
HOGENOMi | CLU_012348_1_2_2 |
InParanoidi | Q97W02 |
OMAi | CKPDGLL |
PhylomeDBi | Q97W02 |
Enzyme and pathway databases
BRENDAi | 2.7.7.7, 6163 |
Miscellaneous databases
EvolutionaryTracei | Q97W02 |
Family and domain databases
CDDi | cd03586, PolY_Pol_IV_kappa, 1 hit |
Gene3Di | 3.30.1490.100, 1 hit 3.30.70.270, 1 hit |
HAMAPi | MF_01113, DNApol_IV, 1 hit |
InterProi | View protein in InterPro IPR043502, DNA/RNA_pol_sf IPR036775, DNA_pol_Y-fam_lit_finger_sf IPR017961, DNA_pol_Y-fam_little_finger IPR022880, DNApol_IV IPR024728, PolY_HhH_motif IPR043128, Rev_trsase/Diguanyl_cyclase IPR001126, UmuC |
Pfami | View protein in Pfam PF00817, IMS, 1 hit PF11799, IMS_C, 1 hit PF11798, IMS_HHH, 1 hit |
SUPFAMi | SSF100879, SSF100879, 1 hit SSF56672, SSF56672, 1 hit |
PROSITEi | View protein in PROSITE PS50173, UMUC, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DPO4_SACS2 | |
Accessioni | Q97W02Primary (citable) accession number: Q97W02 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 2, 2002 |
Last sequence update: | October 1, 2001 | |
Last modified: | February 23, 2022 | |
This is version 154 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families