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Protein

L-lactate dehydrogenase 1

Gene

ldh1

Organism
Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of lactate to pyruvate.UniRule annotation

Catalytic activityi

(S)-lactate + NAD+ = pyruvate + NADH.UniRule annotation

Activity regulationi

Allosterically activated by fructose 1,6-bisphosphate (FBP).UniRule annotation

Pathwayi: pyruvate fermentation to lactate

This protein is involved in step 1 of the subpathway that synthesizes (S)-lactate from pyruvate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. L-lactate dehydrogenase 2 (ldh2), L-lactate dehydrogenase 1 (ldh1)
This subpathway is part of the pathway pyruvate fermentation to lactate, which is itself part of Fermentation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-lactate from pyruvate, the pathway pyruvate fermentation to lactate and in Fermentation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei15NAD; via amide nitrogenUniRule annotation1
Binding sitei36NADUniRule annotation1
Binding sitei41NADUniRule annotation1
Binding sitei66NADUniRule annotation1
Binding sitei83SubstrateUniRule annotation1
Binding sitei89SubstrateUniRule annotation1
Binding sitei102NADUniRule annotation1
Binding sitei144NADUniRule annotation1
Binding sitei154Allosteric activatorUniRule annotation1
Binding sitei169Allosteric activatorUniRule annotation1
Active sitei176Proton acceptorUniRule annotation1
Binding sitei231SubstrateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi80 – 81NADUniRule annotation2
Nucleotide bindingi119 – 121NADUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAllosteric enzyme, Oxidoreductase
LigandNAD

Enzyme and pathway databases

UniPathwayi
UPA00554;UER00611

Names & Taxonomyi

Protein namesi
Recommended name:
L-lactate dehydrogenase 1UniRule annotation (EC:1.1.1.27UniRule annotation)
Short name:
L-LDH 1UniRule annotation
Gene namesi
Name:ldh1UniRule annotation
Synonyms:ldh
Ordered Locus Names:CA_C0267
OrganismiClostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)
Taxonomic identifieri272562 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
Proteomesi
  • UP000000814 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001683331 – 313L-lactate dehydrogenase 1Add BLAST313

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei222PhosphotyrosineUniRule annotation1

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi272562.CA_C0267

Structurei

3D structure databases

ProteinModelPortaliQ97MD1
SMRiQ97MD1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni121 – 124Substrate bindingUniRule annotation4
Regioni149 – 152Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. LDH family.UniRule annotationCurated

Phylogenomic databases

eggNOGiENOG4105C80 Bacteria
COG0039 LUCA
HOGENOMiHOG000213793
KOiK00016
OMAiMDLMQTA

Family and domain databases

Gene3Di3.90.110.10, 1 hit
HAMAPiMF_00488 Lactate_dehydrog, 1 hit
InterProiView protein in InterPro
IPR001557 L-lactate/malate_DH
IPR011304 L-lactate_DH
IPR018177 L-lactate_DH_AS
IPR022383 Lactate/malate_DH_C
IPR001236 Lactate/malate_DH_N
IPR015955 Lactate_DH/Glyco_Ohase_4_C
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF02866 Ldh_1_C, 1 hit
PF00056 Ldh_1_N, 1 hit
PIRSFiPIRSF000102 Lac_mal_DH, 1 hit
PRINTSiPR00086 LLDHDRGNASE
SUPFAMiSSF51735 SSF51735, 1 hit
SSF56327 SSF56327, 1 hit
TIGRFAMsiTIGR01771 L-LDH-NAD, 1 hit
PROSITEiView protein in PROSITE
PS00064 L_LDH, 1 hit

Sequencei

Sequence statusi: Complete.

Q97MD1-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKKNTKISVI GAGFVGSSTV FALMNGGLAS EIVIVDVNKD KAEGEAMDLS
60 70 80 90 100
HGAAFVKPVV VKSGDYKDTE GSDIVIITAG AAQKPGETRL ELINKNYNIF
110 120 130 140 150
KSIVPEVVKY NPNAILLVVS NPVDILTYIT YKLSGFPKSR VIGSGTVLDT
160 170 180 190 200
SRFRYMLSEH FEIDVRNIHT YIMGEHGDSE IATWSLTNIA GMDVNEYCEA
210 220 230 240 250
SCKKCDGSLK YKIYDDVKNA AYHVIEKKGA TYYAVALAVK RIVEAILRDE
260 270 280 290 300
NSILTVSSLL EGQYGIKDVY MGVPSIVGIN GVKDIIEVPL NDEEKNNLTD
310
SAKTLKESLD SIF
Length:313
Mass (Da):34,181
Last modified:October 1, 2001 - v1
Checksum:iC98FD402E62F45D2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001437 Genomic DNA Translation: AAK78248.1
PIRiE96932
RefSeqiNP_346908.1, NC_003030.1
WP_010963590.1, NC_003030.1

Genome annotation databases

EnsemblBacteriaiAAK78248; AAK78248; CA_C0267
GeneIDi1116450
KEGGicac:CA_C0267
PATRICifig|272562.8.peg.453

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001437 Genomic DNA Translation: AAK78248.1
PIRiE96932
RefSeqiNP_346908.1, NC_003030.1
WP_010963590.1, NC_003030.1

3D structure databases

ProteinModelPortaliQ97MD1
SMRiQ97MD1
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272562.CA_C0267

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK78248; AAK78248; CA_C0267
GeneIDi1116450
KEGGicac:CA_C0267
PATRICifig|272562.8.peg.453

Phylogenomic databases

eggNOGiENOG4105C80 Bacteria
COG0039 LUCA
HOGENOMiHOG000213793
KOiK00016
OMAiMDLMQTA

Enzyme and pathway databases

UniPathwayi
UPA00554;UER00611

Family and domain databases

Gene3Di3.90.110.10, 1 hit
HAMAPiMF_00488 Lactate_dehydrog, 1 hit
InterProiView protein in InterPro
IPR001557 L-lactate/malate_DH
IPR011304 L-lactate_DH
IPR018177 L-lactate_DH_AS
IPR022383 Lactate/malate_DH_C
IPR001236 Lactate/malate_DH_N
IPR015955 Lactate_DH/Glyco_Ohase_4_C
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF02866 Ldh_1_C, 1 hit
PF00056 Ldh_1_N, 1 hit
PIRSFiPIRSF000102 Lac_mal_DH, 1 hit
PRINTSiPR00086 LLDHDRGNASE
SUPFAMiSSF51735 SSF51735, 1 hit
SSF56327 SSF56327, 1 hit
TIGRFAMsiTIGR01771 L-LDH-NAD, 1 hit
PROSITEiView protein in PROSITE
PS00064 L_LDH, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiLDH1_CLOAB
AccessioniPrimary (citable) accession number: Q97MD1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: October 1, 2001
Last modified: October 10, 2018
This is version 115 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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