UniProtKB - Q96WZ5 (ACEA_TALMA)
Protein
Isocitrate lyase
Gene
icl1
Organism
Talaromyces marneffei (Penicillium marneffei)
Status
Functioni
Catalyzes the formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle, which operates as an anaplerotic route for replenishing the tricarboxylic acid cycle. Required for growth on ethanol or acetate, but dispensable when fermentable carbon sources are available. Acts also on 2-methylisocitrate.By similarity
Catalytic activityi
Cofactori
Mg2+By similarity
: glyoxylate cycle Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes (S)-malate from isocitrate.Proteins known to be involved in the 2 steps of the subpathway in this organism are:
- Isocitrate lyase (icl1)
- no protein annotated in this organism
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 170 | MagnesiumBy similarity | 1 | |
Active sitei | 208 | Proton acceptorBy similarity | 1 | |
Binding sitei | 245 | SubstrateBy similarity | 1 | |
Binding sitei | 459 | SubstrateBy similarity | 1 |
GO - Molecular functioni
- isocitrate lyase activity Source: UniProtKB-EC
- metal ion binding Source: UniProtKB-KW
- methylisocitrate lyase activity Source: UniProtKB-EC
GO - Biological processi
- glyoxylate cycle Source: UniProtKB-UniPathway
- tricarboxylic acid cycle Source: UniProtKB-KW
Keywordsi
Molecular function | Lyase |
Biological process | Glyoxylate bypass, Tricarboxylic acid cycle |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
UniPathwayi | UPA00703;UER00719 |
Names & Taxonomyi
Protein namesi | Recommended name: Isocitrate lyaseBy similarity (EC:4.1.3.1By similarity)Short name: ICLCurated Short name: IsocitraseCurated Short name: IsocitrataseCurated Alternative name(s): Threo-D(S)-isocitrate glyoxylate-lyaseCurated |
Gene namesi | Name:icl1By similarity |
Organismi | Talaromyces marneffei (Penicillium marneffei) |
Taxonomic identifieri | 37727 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › Talaromyces › Talaromyces sect. Talaromyces |
Subcellular locationi
Peroxisome
- Glyoxysome By similarity
Peroxisome
- glyoxysome Source: UniProtKB-SubCell
Keywords - Cellular componenti
Glyoxysome, PeroxisomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000068795 | 1 – 540 | Isocitrate lyaseAdd BLAST | 540 |
Interactioni
Subunit structurei
Homotetramer.
By similarityFamily & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 99 – 101 | Substrate bindingBy similarity | 3 | |
Regioni | 209 – 210 | Substrate bindingBy similarity | 2 | |
Regioni | 425 – 429 | Substrate bindingBy similarity | 5 |
Sequence similaritiesi
Family and domain databases
Gene3Di | 3.20.20.60, 1 hit |
InterProi | View protein in InterPro IPR006254, Isocitrate_lyase IPR018523, Isocitrate_lyase_ph_CS IPR015813, Pyrv/PenolPyrv_Kinase-like_dom IPR040442, Pyrv_Kinase-like_dom_sf |
PANTHERi | PTHR21631, PTHR21631, 1 hit |
Pfami | View protein in Pfam PF00463, ICL, 1 hit |
PIRSFi | PIRSF001362, Isocit_lyase, 1 hit |
SUPFAMi | SSF51621, SSF51621, 1 hit |
TIGRFAMsi | TIGR01346, isocit_lyase, 1 hit |
PROSITEi | View protein in PROSITE PS00161, ISOCITRATE_LYASE, 1 hit |
i Sequence
Sequence statusi: Complete.
Q96WZ5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MASTNFDLED QKYLEDVNAV KQWWTDSRWR YTKRPFTAEQ IVAKRGTLKI
60 70 80 90 100
EYPSNTQAKK LWKILEERFN DKTVSYTYGC LEPTMLTQMI KYLDTIYVSG
110 120 130 140 150
WQSSSTASST DEPSPDLADY PMNTVPNKVN QLFMAQLFHD RKQREERITT
160 170 180 190 200
PREKRSSVQN YDYLRPIIAD ADTGHGGLTA VMKLTKLFVE RGAAGIHIED
210 220 230 240 250
QAPGTKKCGH MAGKVLVPIS EHINRLVAIR AQADIMGTDL LAIARTDSEA
260 270 280 290 300
ATLITSTIDY RDHAYLLGST NPSLQPLNDL MVAAEQSGKS GEQLQAIEDS
310 320 330 340 350
WIAQAGIKKF DDAVIDTINQ GSFANKKELI NRYLTAAKGK SNSEARAIAK
360 370 380 390 400
GITGMDIYWN WDAPRTRGGF YRYQGGTQCA VNRAVAYAPF ADLIWMESKL
410 420 430 440 450
PDYKQAKEFA DGVHAVWPEQ KLAYNLSPSF NWKAAMSKEE QLTYIKRLGK
460 470 480 490 500
LGYCWQFITL AGLHSTALIS DQFASAYAKQ GMRAYGELIQ EPEMERKVDI
510 520 530 540
VTHQKWSGAN LIDHSLAMVT GGISSTAAMG KGVTEDQFKS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF373018 Genomic DNA Translation: AAK54240.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF373018 Genomic DNA Translation: AAK54240.1 |
3D structure databases
SMRi | Q96WZ5 |
ModBasei | Search... |
Enzyme and pathway databases
UniPathwayi | UPA00703;UER00719 |
Family and domain databases
Gene3Di | 3.20.20.60, 1 hit |
InterProi | View protein in InterPro IPR006254, Isocitrate_lyase IPR018523, Isocitrate_lyase_ph_CS IPR015813, Pyrv/PenolPyrv_Kinase-like_dom IPR040442, Pyrv_Kinase-like_dom_sf |
PANTHERi | PTHR21631, PTHR21631, 1 hit |
Pfami | View protein in Pfam PF00463, ICL, 1 hit |
PIRSFi | PIRSF001362, Isocit_lyase, 1 hit |
SUPFAMi | SSF51621, SSF51621, 1 hit |
TIGRFAMsi | TIGR01346, isocit_lyase, 1 hit |
PROSITEi | View protein in PROSITE PS00161, ISOCITRATE_LYASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ACEA_TALMA | |
Accessioni | Q96WZ5Primary (citable) accession number: Q96WZ5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 11, 2004 |
Last sequence update: | December 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 77 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Documents
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families