UniProtKB - Q96WV1 (TRMB_SCHPO)
Protein
tRNA (guanine-N(7)-)-methyltransferase
Gene
trm8
Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Functioni
Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation
Catalytic activityi
- guanosine46 in tRNA + S-adenosyl-L-methionine = N7-methylguanosine46 in tRNA + S-adenosyl-L-homocysteineUniRule annotationEC:2.1.1.33UniRule annotation
: N(7)-methylguanine-tRNA biosynthesis Pathwayi
This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotationView all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 88 | S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation | 1 | |
Binding sitei | 170 | S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation | 1 | |
Active sitei | 173 | UniRule annotation | 1 |
GO - Molecular functioni
- tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB
- tRNA binding Source: PomBase
GO - Biological processi
- RNA (guanine-N7)-methylation Source: GO_Central
- tRNA (guanine-N7)-methylation Source: PomBase
- tRNA methylation Source: GO_Central
- tRNA modification Source: UniProtKB
Keywordsi
Molecular function | Methyltransferase, RNA-binding, Transferase, tRNA-binding |
Biological process | tRNA processing |
Ligand | S-adenosyl-L-methionine |
Enzyme and pathway databases
UniPathwayi | UPA00989 |
Names & Taxonomyi
Protein namesi | Recommended name: tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)Alternative name(s): Transfer RNA methyltransferase 8UniRule annotation tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation tRNA(m7G46)-methyltransferaseUniRule annotation |
Gene namesi | Name:trm8 ORF Names:SPCPB16A4.04c |
Organismi | Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) |
Taxonomic identifieri | 284812 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › |
Proteomesi |
|
Organism-specific databases
PomBasei | SPCPB16A4.04c, trm8 |
VEuPathDBi | FungiDB:SPCPB16A4.04c |
Subcellular locationi
Nucleus
- Nucleus UniRule annotation
Nucleus
Other locations
- tRNA (m7G46) methyltransferase complex Source: PomBase
- tRNA methyltransferase complex Source: GO_Central
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000171436 | 1 – 273 | tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST | 273 |
Proteomic databases
MaxQBi | Q96WV1 |
PaxDbi | Q96WV1 |
PRIDEi | Q96WV1 |
Interactioni
Subunit structurei
Forms a complex with trm82.
UniRule annotationProtein-protein interaction databases
BioGRIDi | 276134, 17 interactors |
STRINGi | 4896.SPCPB16A4.04c.1 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 111 – 112 | S-adenosyl-L-methionine bindingUniRule annotation | 2 | |
Regioni | 150 – 151 | S-adenosyl-L-methionine bindingUniRule annotation | 2 | |
Regioni | 248 – 250 | S-adenosyl-L-methionine bindingUniRule annotation | 3 |
Sequence similaritiesi
Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation
Phylogenomic databases
eggNOGi | KOG3115, Eukaryota |
HOGENOMi | CLU_050910_3_1_1 |
InParanoidi | Q96WV1 |
OMAi | KWRIINQ |
PhylomeDBi | Q96WV1 |
Family and domain databases
HAMAPi | MF_03055, tRNA_methyltr_TrmB_euk, 1 hit |
InterProi | View protein in InterPro IPR029063, SAM-dependent_MTases IPR025763, Trm8_euk IPR003358, tRNA_(Gua-N-7)_MeTrfase_Trmb |
PANTHERi | PTHR23417, PTHR23417, 1 hit PTHR23417:SF16, PTHR23417:SF16, 1 hit |
Pfami | View protein in Pfam PF02390, Methyltransf_4, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
TIGRFAMsi | TIGR00091, TIGR00091, 1 hit |
PROSITEi | View protein in PROSITE PS51625, SAM_MT_TRMB, 1 hit |
i Sequence
Sequence statusi: Complete.
Q96WV1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSATAKKSAA QLQREEEEAR KKLKRLSKQG GVEGRLPMKR LFRQRAHANV
60 70 80 90 100
LSDHELEYPR SPSEMDWSPY YPDFDVESNK KVEIVDIGCG YGGLTVALGP
110 120 130 140 150
QFPDTLVLGM EIRMQVSDYL KEKIQALRYR ADHEEPVPGG YKNISVLRMN
160 170 180 190 200
CQKFLPNFFE KGQLSKMFFC FPDPHFKARK HKNRIITSTL ASEYAYFIRP
210 220 230 240 250
HGTLYTITDV EELHVWMAQH LDAHPLFRRF TKEEEENDIC VTLMTNETEE
260 270
GKKVARNGGK KFVACYERIP NPK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CU329672 Genomic DNA Translation: CAC39323.1 |
RefSeqi | NP_588028.1, NM_001023019.2 |
Genome annotation databases
EnsemblFungii | SPCPB16A4.04c.1; SPCPB16A4.04c.1:pep; SPCPB16A4.04c |
GeneIDi | 2539574 |
KEGGi | spo:SPCPB16A4.04c |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CU329672 Genomic DNA Translation: CAC39323.1 |
RefSeqi | NP_588028.1, NM_001023019.2 |
3D structure databases
SMRi | Q96WV1 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 276134, 17 interactors |
STRINGi | 4896.SPCPB16A4.04c.1 |
Proteomic databases
MaxQBi | Q96WV1 |
PaxDbi | Q96WV1 |
PRIDEi | Q96WV1 |
Genome annotation databases
EnsemblFungii | SPCPB16A4.04c.1; SPCPB16A4.04c.1:pep; SPCPB16A4.04c |
GeneIDi | 2539574 |
KEGGi | spo:SPCPB16A4.04c |
Organism-specific databases
PomBasei | SPCPB16A4.04c, trm8 |
VEuPathDBi | FungiDB:SPCPB16A4.04c |
Phylogenomic databases
eggNOGi | KOG3115, Eukaryota |
HOGENOMi | CLU_050910_3_1_1 |
InParanoidi | Q96WV1 |
OMAi | KWRIINQ |
PhylomeDBi | Q96WV1 |
Enzyme and pathway databases
UniPathwayi | UPA00989 |
Miscellaneous databases
PROi | PR:Q96WV1 |
Family and domain databases
HAMAPi | MF_03055, tRNA_methyltr_TrmB_euk, 1 hit |
InterProi | View protein in InterPro IPR029063, SAM-dependent_MTases IPR025763, Trm8_euk IPR003358, tRNA_(Gua-N-7)_MeTrfase_Trmb |
PANTHERi | PTHR23417, PTHR23417, 1 hit PTHR23417:SF16, PTHR23417:SF16, 1 hit |
Pfami | View protein in Pfam PF02390, Methyltransf_4, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
TIGRFAMsi | TIGR00091, TIGR00091, 1 hit |
PROSITEi | View protein in PROSITE PS51625, SAM_MT_TRMB, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TRMB_SCHPO | |
Accessioni | Q96WV1Primary (citable) accession number: Q96WV1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 20, 2003 |
Last sequence update: | December 1, 2001 | |
Last modified: | February 10, 2021 | |
This is version 123 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Schizosaccharomyces pombe
Schizosaccharomyces pombe: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families