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Entry version 50 (07 Apr 2021)
Sequence version 1 (01 Dec 2001)
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Protein

Aphidicolan-16-beta-ol synthase

Gene

ACS

Organism
Pleospora betae (Beet black rot fungus) (Phoma betae)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Aphidicolan-16-beta-ol synthase; part of the gene cluster that mediates the biosynthesis of aphidicolin, a specific inhibitor of eukaryotic DNA synthesis and DNA polymerase alpha (PubMed:14745177, PubMed:21897020).

The geranylgeranyl pyrophosphate synthase GGS is required for supplying a sufficient amount of geranylgeranyl diphosphate (GGDP), the general precursor of diterpenes (PubMed:21897020).

The diterpene synthase ACS then catalyzes the conversion of geranylgeranyl diphosphate to aphidicolan-16-beta-ol via the intermediate syn-copalyldiphosphate (syn-CDP) (PubMed:11457369, PubMed:21897020).

In addition to aphidicolan-16-beta-ol, the enzyme produces also low levels of amphidicol-15-ene and amphidicol-16-ene (PubMed:11457369).

The cytochrome P450 monooxygenase P450-2 then catalyzes the two-step hydroxylation from aphidicolan-16-beta-ol to 3-deoxyaphidicolin via a 17,3-deoxyaphidicolin intermediate (PubMed:21897020).

Finally, the cytochrome P450 monooxygenase P450-1 converts 3-deoxyaphidicolin to aphidicolin (PubMed:21897020).

3 Publications

Miscellaneous

Part of a gene cluster that groups the genes involved in aphidicolin biosynthesis.

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Mg2+By similarityNote: Binds 3 Mg2+ ions per subunit.By similarity

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: Mycotoxin biosynthesis

This protein is involved in Mycotoxin biosynthesis.1 Publication
View all proteins of this organism that are known to be involved in Mycotoxin biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the 'Description' field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi323Magnesium 4By similarity1
Metal bindingi325Magnesium 4By similarity1
Metal bindingi661Magnesium 1By similarity1
Metal bindingi661Magnesium 2By similarity1
Metal bindingi665Magnesium 1By similarity1
Metal bindingi665Magnesium 2By similarity1
Metal bindingi835Magnesium 3By similarity1
Metal bindingi839Magnesium 3By similarity1
Metal bindingi843Magnesium 3By similarity1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionIsomerase, Lyase
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

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BioCyci
MetaCyc:MONOMER-14877

BRENDA Comprehensive Enzyme Information System

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BRENDAi
4.2.3.42, 10692

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Aphidicolan-16-beta-ol synthase1 Publication (EC:4.2.3.421 Publication, EC:5.5.1.141 Publication)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:ACS
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiPleospora betae (Beet black rot fungus) (Phoma betae)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri137527 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaDothideomycetesPleosporomycetidaePleosporalesPleosporineaeDidymellaceaePhoma

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00004188121 – 944Aphidicolan-16-beta-ol synthaseAdd BLAST944

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a short (usually not more than 20 amino acids) conserved sequence motif of biological significance.<p><a href='/help/motif' target='_top'>More...</a></p>Motifi323 – 326DXDD motif4
Motifi661 – 665DEXXE motifBy similarity5

<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

The DXDD and DEXXE motifs are important for the catalytic activity.By similarity

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the terpene synthase family.Curated

Family and domain databases

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR017057, Ent-kaurene_synthase_fun
IPR008930, Terpenoid_cyclase/PrenylTrfase

PIRSF; a whole-protein classification database

More...
PIRSFi
PIRSF036498, Ent-kaurene_synthase_fungi, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF48239, SSF48239, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

Q96WT2-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MVPISTRSEV QDGLIAQARS LITRIVHNSD DVYGFGTLSC TVYDTAWVAL
60 70 80 90 100
VTKHVNGIKH WLFPESFHYI LASQCDDGTW CEDKTAQFDG VLNTIAGLLV
110 120 130 140 150
LKRYRRDPLQ LKVDNRDLDS RIKLATSALH SLLEEWDVST TNNVGFEIIV
160 170 180 190 200
PTMLDLLVQE DPSLSFELKG REALTEIREA KMNRFQPELL YQGKLMTLTH
210 220 230 240 250
SLEALLGRID YDNVARYTVK GSMFASPSST AAYLMSASTW DDEAETYLRY
260 270 280 290 300
TITASTGKGS GGVPGVFPTT YFEYTWILST LFRAGFQSSE LDSPELTAMT
310 320 330 340 350
DTLLKAFKAF SGAIGLDSGI EPDVDDSAKI VTTLNMLGKP AHARYLCDNF
360 370 380 390 400
EVETHFRVYP RERDPSFSAN CNALAAFLHQ PDVEVYSSQI LKAASYLCER
410 420 430 440 450
MWNADEKIDD KWNKSHLYSS FLYTQVMTDL MAITEAGRLD GVFTRELLTR
460 470 480 490 500
VCVTIFQSCL RAMLKQSHDG SWNQSLEETA YAILHLTEAR RLCFFEQISE
510 520 530 540 550
PLENSIYRGI TFLTTIDKPP MEYLWSDKVS YGSAYLAETY VLAARRAAES
560 570 580 590 600
TSVTNLVGSS IWKDNASTKM HKLVGLFHRT PLLKALPKWE LQASMIEASI
610 620 630 640 650
YQGLLQDARL EVLQRPKVDG GEYLSIIPFT WTSCSNSART NASASHLWEL
660 670 680 690 700
MALSFFTYQV DEFMEAVAGP AFKGRMTHLH AIIDEAVHCS QNRERTPGEC
710 720 730 740 750
ENTNHVTSEL LQAVRFILDN PTVRKASPYD RNTLLQELRI FLHAHVTQVE
760 770 780 790 800
DNASFGRERL TGDKALTSYR SQLYRWVHTI SSDHIAGPFC FYYATCLLGA
810 820 830 840 850
TLTAHPPNDC FPKSSQKYLA AATCRHLSCM CRMYNDIGSW NRDHREGNLN
860 870 880 890 900
CLHFPEFSET TSDDAERKAS LLTLAQYERK QWCNALQQLE KEMVHGASEP
910 920 930 940
AAARLAKRRA CLIDMFCKVT DLYGQIYVLR DVSSVIKDVV RNGE
Length:944
Mass (Da):106,581
Last modified:December 1, 2001 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i41C781606F236CE5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti445R → K in BAD29971 (PubMed:14745177).Curated1
Sequence conflicti559S → F in BAD29971 (PubMed:14745177).Curated1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AB049075 mRNA Translation: BAB62102.1
AB114137 Genomic DNA Translation: BAD29971.1

Genome annotation databases

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
ag:BAB62102

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB049075 mRNA Translation: BAB62102.1
AB114137 Genomic DNA Translation: BAD29971.1

3D structure databases

Database of comparative protein structure models

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ModBasei
Search...

SWISS-MODEL Interactive Workspace

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SWISS-MODEL-Workspacei
Submit a new modelling project...

Genome annotation databases

KEGGiag:BAB62102

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14877
BRENDAi4.2.3.42, 10692

Family and domain databases

InterProiView protein in InterPro
IPR017057, Ent-kaurene_synthase_fun
IPR008930, Terpenoid_cyclase/PrenylTrfase
PIRSFiPIRSF036498, Ent-kaurene_synthase_fungi, 1 hit
SUPFAMiSSF48239, SSF48239, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiACS_PLEBE
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q96WT2
Secondary accession number(s): Q6F5E5
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 5, 2012
Last sequence update: December 1, 2001
Last modified: April 7, 2021
This is version 50 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
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