UniProtKB - Q96T53 (MBOA4_HUMAN)
Protein
Ghrelin O-acyltransferase
Gene
MBOAT4
Organism
Homo sapiens (Human)
Status
Functioni
Mediates the octanoylation of ghrelin at 'Ser-3'. Can use a variety of fatty acids as substrates including octanoic acid, decanoic acid and tetradecanoic acid.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 307 | By similarity | 1 | |
Active sitei | 338 | Curated | 1 |
GO - Molecular functioni
- O-acyltransferase activity Source: Reactome
- serine O-acyltransferase activity Source: UniProtKB
- transferase activity, transferring acyl groups Source: GO_Central
GO - Biological processi
- lipid modification Source: GO_Central
- peptidyl-serine octanoylation Source: UniProtKB
Keywordsi
Molecular function | Acyltransferase, Transferase |
Enzyme and pathway databases
PathwayCommonsi | Q96T53 |
Reactomei | R-HSA-422085, Synthesis, secretion, and deacylation of Ghrelin |
Protein family/group databases
TCDBi | 2.A.50.2.6, the glycerol uptake (gup) or membrane-bound acyl transfeerase (mboat) family |
Chemistry databases
SwissLipidsi | SLP:000001956 |
Names & Taxonomyi
Protein namesi | Recommended name: Ghrelin O-acyltransferase (EC:2.3.1.-)Alternative name(s): Membrane-bound O-acyltransferase domain-containing protein 4 Short name: O-acyltransferase domain-containing protein 4 |
Gene namesi | Name:MBOAT4 Synonyms:GOAT, OACT4 ORF Names:FKSG89 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:32311, MBOAT4 |
MIMi | 611940, gene |
neXtProti | NX_Q96T53 |
VEuPathDBi | HostDB:ENSG00000177669.3 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane By similarity; Multi-pass membrane protein By similarity
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: Reactome
- integral component of endoplasmic reticulum membrane Source: UniProtKB
Other locations
- membrane Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 6 – 26 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 43 – 63 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 163 – 183 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 241 – 261 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 345 – 365 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 377 – 397 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 407 – 427 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 338 | H → A: Abolishes ability to acylate ghrelin. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 619373 |
OpenTargetsi | ENSG00000177669 |
PharmGKBi | PA142671233 |
Miscellaneous databases
Pharosi | Q96T53, Tbio |
Chemistry databases
ChEMBLi | CHEMBL3588729 |
Genetic variation databases
BioMutai | MBOAT4 |
DMDMi | 182676418 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000273024 | 1 – 435 | Ghrelin O-acyltransferaseAdd BLAST | 435 |
Proteomic databases
jPOSTi | Q96T53 |
PaxDbi | Q96T53 |
PeptideAtlasi | Q96T53 |
PRIDEi | Q96T53 |
ProteomicsDBi | 78189 [Q96T53-1] 78190 [Q96T53-2] |
PTM databases
PhosphoSitePlusi | Q96T53 |
Expressioni
Tissue specificityi
Expressed predominantly in stomach with moderate levels in pancreas and relatively low levels in most other tissues.1 Publication
Gene expression databases
Bgeei | ENSG00000177669, Expressed in islet of Langerhans and 68 other tissues |
Organism-specific databases
HPAi | ENSG00000177669, Tissue enhanced (pancreas, stomach) |
Interactioni
Protein-protein interaction databases
STRINGi | 9606.ENSP00000314196 |
Chemistry databases
BindingDBi | Q96T53 |
Miscellaneous databases
RNActi | Q96T53, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the membrane-bound acyltransferase family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG2704, Eukaryota |
GeneTreei | ENSGT01010000222502 |
HOGENOMi | CLU_011340_3_1_1 |
InParanoidi | Q96T53 |
OMAi | MDWLQLF |
OrthoDBi | 881262at2759 |
PhylomeDBi | Q96T53 |
TreeFami | TF314906 |
Family and domain databases
InterProi | View protein in InterPro IPR004299, MBOAT_fam |
Pfami | View protein in Pfam PF03062, MBOAT, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: Q96T53-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MEWLWLFFLH PISFYQGAAF PFALLFNYLC IMDSFSTRAR YLFLLTGGGA
60 70 80 90 100
LAVAAMGSYA VLVFTPAVCA VALLCSLAPQ QVHRWTFCFQ MSWQTLCHLG
110 120 130 140 150
LHYTEYYLHE PPSVRFCITL SSLMLLTQRV TSLSLDICEG KVKAASGGFR
160 170 180 190 200
SRSSLSEHVC KALPYFSYLL FFPALLGGSL CSFQRFQARV QGSSALHPRH
210 220 230 240 250
SFWALSWRGL QILGLECLNV AVSRVVDAGA GLTDCQQFEC IYVVWTTAGL
260 270 280 290 300
FKLTYYSHWI LDDSLLHAAG FGPELGQSPG EEGYVPDADI WTLERTHRIS
310 320 330 340 350
VFSRKWNQST ARWLRRLVFQ HSRAWPLLQT FAFSAWWHGL HPGQVFGFVC
360 370 380 390 400
WAVMVEADYL IHSFANEFIR SWPMRLFYRT LTWAHTQLII AYIMLAVEVR
410 420 430
SLSSLWLLCN SYNSVFPMVY CILLLLLAKR KHKCN
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_059434 | 46 | T → A1 PublicationCorresponds to variant dbSNP:rs7813902Ensembl. | 1 | |
Natural variantiVAR_030069 | 231 | G → E. Corresponds to variant dbSNP:rs16876563Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_032958 | 1 – 106 | Missing in isoform 2. 1 PublicationAdd BLAST | 106 | |
Alternative sequenceiVSP_032959 | 107 – 114 | YLHEPPSV → MFFKKLSC in isoform 2. 1 Publication | 8 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | EU518495 mRNA Translation: ACB05873.2 AF359269 mRNA Translation: AAK43717.1 AC026979 Genomic DNA No translation available. |
CCDSi | CCDS47835.1 [Q96T53-1] |
RefSeqi | NP_001094386.1, NM_001100916.1 [Q96T53-1] XP_016869215.1, XM_017013726.1 [Q96T53-1] |
Genome annotation databases
Ensembli | ENST00000320542; ENSP00000314196; ENSG00000177669 [Q96T53-1] |
GeneIDi | 619373 |
KEGGi | hsa:619373 |
UCSCi | uc010lvg.4, human [Q96T53-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | EU518495 mRNA Translation: ACB05873.2 AF359269 mRNA Translation: AAK43717.1 AC026979 Genomic DNA No translation available. |
CCDSi | CCDS47835.1 [Q96T53-1] |
RefSeqi | NP_001094386.1, NM_001100916.1 [Q96T53-1] XP_016869215.1, XM_017013726.1 [Q96T53-1] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 9606.ENSP00000314196 |
Chemistry databases
BindingDBi | Q96T53 |
ChEMBLi | CHEMBL3588729 |
SwissLipidsi | SLP:000001956 |
Protein family/group databases
TCDBi | 2.A.50.2.6, the glycerol uptake (gup) or membrane-bound acyl transfeerase (mboat) family |
PTM databases
PhosphoSitePlusi | Q96T53 |
Genetic variation databases
BioMutai | MBOAT4 |
DMDMi | 182676418 |
Proteomic databases
jPOSTi | Q96T53 |
PaxDbi | Q96T53 |
PeptideAtlasi | Q96T53 |
PRIDEi | Q96T53 |
ProteomicsDBi | 78189 [Q96T53-1] 78190 [Q96T53-2] |
Protocols and materials databases
Antibodypediai | 54984, 209 antibodies |
Genome annotation databases
Ensembli | ENST00000320542; ENSP00000314196; ENSG00000177669 [Q96T53-1] |
GeneIDi | 619373 |
KEGGi | hsa:619373 |
UCSCi | uc010lvg.4, human [Q96T53-1] |
Organism-specific databases
CTDi | 619373 |
DisGeNETi | 619373 |
GeneCardsi | MBOAT4 |
HGNCi | HGNC:32311, MBOAT4 |
HPAi | ENSG00000177669, Tissue enhanced (pancreas, stomach) |
MIMi | 611940, gene |
neXtProti | NX_Q96T53 |
OpenTargetsi | ENSG00000177669 |
PharmGKBi | PA142671233 |
VEuPathDBi | HostDB:ENSG00000177669.3 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2704, Eukaryota |
GeneTreei | ENSGT01010000222502 |
HOGENOMi | CLU_011340_3_1_1 |
InParanoidi | Q96T53 |
OMAi | MDWLQLF |
OrthoDBi | 881262at2759 |
PhylomeDBi | Q96T53 |
TreeFami | TF314906 |
Enzyme and pathway databases
PathwayCommonsi | Q96T53 |
Reactomei | R-HSA-422085, Synthesis, secretion, and deacylation of Ghrelin |
Miscellaneous databases
BioGRID-ORCSi | 619373, 6 hits in 871 CRISPR screens |
ChiTaRSi | MBOAT4, human |
GenomeRNAii | 619373 |
Pharosi | Q96T53, Tbio |
PROi | PR:Q96T53 |
RNActi | Q96T53, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000177669, Expressed in islet of Langerhans and 68 other tissues |
Family and domain databases
InterProi | View protein in InterPro IPR004299, MBOAT_fam |
Pfami | View protein in Pfam PF03062, MBOAT, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MBOA4_HUMAN | |
Accessioni | Q96T53Primary (citable) accession number: Q96T53 Secondary accession number(s): B1Q003 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 23, 2007 |
Last sequence update: | April 8, 2008 | |
Last modified: | February 10, 2021 | |
This is version 110 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 8
Human chromosome 8: entries, gene names and cross-references to MIM - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Human entries with genetic variants
List of human entries with genetic variants