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UniProtKB - Q96SZ6 (CK5P1_HUMAN)

Entry version 172 (22 Apr 2020)
Sequence version 2 (16 Jun 2003)
Previous versions | rss
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Protein

Mitochondrial tRNA methylthiotransferase CDK5RAP1

Gene

CDK5RAP1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Methylthiotransferase that catalyzes the conversion of N6-(dimethylallyl)adenosine (i6A) to 2-methylthio-N6-(dimethylallyl)adenosine (ms2i6A) at position 37 (adjacent to the 3'-end of the anticodon) of four mitochondrial DNA-encoded tRNAs (Ser(UCN), Phe, Tyr and Trp) (PubMed:22422838, PubMed:25738458, PubMed:28981754). Essential for efficient and highly accurate protein translation by the ribosome (PubMed:22422838, PubMed:25738458, PubMed:28981754). Specifically inhibits CDK5 activation by CDK5R1 (PubMed:11882646). Essential for efficient mitochondrial protein synthesis and respiratory chain; shows pathological consequences in mitochondrial disease (PubMed:25738458).4 Publications

Caution

CDK5RAP1 was proposed to act on both nuclear and mitochondrial RNA (PubMed:22422838). However, another study shows that ms2i6A is a mitochondrial tRNA specific modification and is absent from nuclear encoded RNA species, implying that there is no methylthiotransferase activity on nuclear RNA (PubMed:28981754).2 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

[4Fe-4S] clusterPROSITE-ProRule annotationNote: Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the 'Description' field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi109Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi145Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi183Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi258Iron-sulfur (4Fe-4S-S-AdoMet)By similarity1
Metal bindingi262Iron-sulfur (4Fe-4S-S-AdoMet)By similarity1
Metal bindingi265Iron-sulfur (4Fe-4S-S-AdoMet)By similarity1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionTransferase
Biological processtRNA processing
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Mitochondrial tRNA methylthiotransferase CDK5RAP12 Publications (EC:2.8.4.32 Publications)
Alternative name(s):
CDK5 activator-binding protein C42
CDK5 regulatory subunit-associated protein 1
mt-tRNA-2-methylthio-N6-dimethylallyladenosine synthase
mt-tRNA-N6-(dimethylallyl)adenosine(37) methylthiotransferase
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:CDK5RAP1Imported
Synonyms:C20orf34
ORF Names:CGI-05, HSPC167
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiHomo sapiens (Human)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri9606 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000005640 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 20

Organism-specific databases

Human Gene Nomenclature Database

More...HGNCi		HGNC:15880 CDK5RAP1

Online Mendelian Inheritance in Man (OMIM)

More...MIMi		608200 gene

neXtProt; the human protein knowledge platform

More...neXtProti		NX_Q96SZ6

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi258C → A: Loss of mitochondrial tRNAs methylthiotransferase activity; when associated with A-262 and A-265. 1 Publication1
Mutagenesisi262C → A: Loss of mitochondrial tRNAs methylthiotransferase activity; when associated with A-258 and A-265. 1 Publication1
Mutagenesisi265C → A: Loss of mitochondrial tRNAs methylthiotransferase activity; when associated with A-258 and A-262. 1 Publication1

Organism-specific databases

DisGeNET

More...DisGeNETi		51654

Open Targets

More...OpenTargetsi		ENSG00000101391

The Pharmacogenetics and Pharmacogenomics Knowledge Base

More...PharmGKBi		PA26313

Miscellaneous databases

Pharos NIH Druggable Genome Knowledgebase

More...Pharosi		Q96SZ6 Tbio

Polymorphism and mutation databases

BioMuta curated single-nucleotide variation and disease association database

More...BioMutai		CDK5RAP1

Domain mapping of disease mutations (DMDM)

More...DMDMi		32129446

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a transit peptide.<p><a href='/help/transit' target='_top'>More...</a></p>Transit peptidei1 – 33MitochondrionSequence analysisAdd BLAST33
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_000014176434 – 601Mitochondrial tRNA methylthiotransferase CDK5RAP1Sequence analysisAdd BLAST568

Proteomic databases

Encyclopedia of Proteome Dynamics

More...EPDi		Q96SZ6

jPOST - Japan Proteome Standard Repository/Database

More...jPOSTi		Q96SZ6

MassIVE - Mass Spectrometry Interactive Virtual Environment

More...MassIVEi		Q96SZ6

MaxQB - The MaxQuant DataBase

More...MaxQBi		Q96SZ6

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		Q96SZ6

PeptideAtlas

More...PeptideAtlasi		Q96SZ6

PRoteomics IDEntifications database

More...PRIDEi		Q96SZ6

ProteomicsDB: a multi-organism proteome resource

More...ProteomicsDBi		65981
78165 [Q96SZ6-1]
78166 [Q96SZ6-2]
78167 [Q96SZ6-3]
78168 [Q96SZ6-4]

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...iPTMneti		Q96SZ6

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

More...PhosphoSitePlusi		Q96SZ6

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified 'at protein level'.<br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas (PubMed:10721722). Expressed in neurons of central nervous tissue (PubMed:10721722, PubMed:11882646).2 Publications
Mainly expressed in brain, placenta and testis.
High expression in placenta and lung.

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...Bgeei		ENSG00000101391 Expressed in small intestine Peyer's patch and 225 other tissues

ExpressionAtlas, Differential and Baseline Expression

More...ExpressionAtlasi		Q96SZ6 baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

More...Genevisiblei		Q96SZ6 HS

Organism-specific databases

Human Protein Atlas

More...HPAi		ENSG00000101391 Low tissue specificity

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Interacts with CDK5R1 (p35 form). CDK5RAP1, CDK5RAP2 and CDK5RAP3 show competitive binding to CDK5R1.

Forms a complex with CDK5R1 and CDK5.

1 Publication

GO - Molecular functioni

Complete GO annotation on QuickGO ...

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGrid)

More...BioGridi		119661, 29 interactors

Protein interaction database and analysis system

More...IntActi		Q96SZ6, 17 interactors

STRING: functional protein association networks

More...STRINGi		9606.ENSP00000217372

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...RNActi		Q96SZ6 protein

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini100 – 220MTTase N-terminalPROSITE-ProRule annotationAdd BLAST121
Domaini515 – 590TRAMPROSITE-ProRule annotationAdd BLAST76

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the methylthiotransferase family. MiaB subfamily.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		KOG2492 Eukaryota
COG0621 LUCA

Ensembl GeneTree

More...GeneTreei		ENSGT00940000160361

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		CLU_018697_2_1_1

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		Q96SZ6

Identification of Orthologs from Complete Genome Data

More...OMAi		CEHFHIP

Database of Orthologous Groups

More...OrthoDBi		835984at2759

Database for complete collections of gene phylogenies

More...PhylomeDBi		Q96SZ6

TreeFam database of animal gene trees

More...TreeFami		TF101033

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...Gene3Di		3.40.50.12160, 1 hit
3.80.30.20, 1 hit

HAMAP database of protein families

More...HAMAPi		MF_01864 tRNA_metthiotr_MiaB, 1 hit

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR006638 Elp3/MiaB/NifB
IPR005839 Methylthiotransferase
IPR020612 Methylthiotransferase_CS
IPR013848 Methylthiotransferase_N
IPR038135 Methylthiotransferase_N_sf
IPR006463 MiaB_methiolase
IPR007197 rSAM
IPR023404 rSAM_horseshoe
IPR002792 TRAM_dom

Pfam protein domain database

More...Pfami		View protein in Pfam
PF04055 Radical_SAM, 1 hit
PF01938 TRAM, 1 hit
PF00919 UPF0004, 1 hit

Structure-Function Linkage Database

More...SFLDi		SFLDF00273 (dimethylallyl)adenosine_tRNA, 1 hit
SFLDS00029 Radical_SAM, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...SMARTi		View protein in SMART
SM00729 Elp3, 1 hit

TIGRFAMs; a protein family database

More...TIGRFAMsi		TIGR00089 TIGR00089, 1 hit

PROSITE; a protein domain and family database

More...PROSITEi		View protein in PROSITE
PS51449 MTTASE_N, 1 hit
PS01278 MTTASE_RADICAL, 1 hit
PS50926 TRAM, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (6+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is in its mature form or if it represents the precursor.<p><a href='/help/sequence_processing' target='_top'>More...</a></p>Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 6 <p>This subsection of the 'Sequence' section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform. This section is only present in reviewed entries, i.e. in UniProtKB/Swiss-Prot.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 6 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q96SZ6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the <div> <p><b>What is the canonical sequence?</b><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MHPLQCVLQV QRSLGWGPLA SVSWLSLRMC RAHSSLSSTM CPSPERQEDG 
        60         70         80         90        100
ARKDFSSRLA AGPTFQHFLK SASAPQEKLS SEVEDPPPYL MMDELLGRQR 
       110        120        130        140        150
KVYLETYGCQ MNVNDTEIAW SILQKSGYLR TSNLQEADVI LLVTCSIREK 
       160        170        180        190        200
AEQTIWNRLH QLKALKTRRP RSRVPLRIGI LGCMAERLKE EILNREKMVD 
       210        220        230        240        250
ILAGPDAYRD LPRLLAVAES GQQAANVLLS LDETYADVMP VQTSASATSA 
       260        270        280        290        300
FVSIMRGCDN MCSYCIVPFT RGRERSRPIA SILEEVKKLS EQVFLPPRPP 
       310        320        330        340        350
KVLGLQGLKE VTLLGQNVNS FRDNSEVQFN SAVPTNLSRG FTTNYKTKQG 
       360        370        380        390        400
GLRFAHLLDQ VSRVDPEMRI RFTSPHPKDF PDEVLQLIHE RDNICKQIHL 
       410        420        430        440        450
PAQSGSSRVL EAMRRGYSRE AYVELVHHIR ESIPGVSLSS DFIAGFCGET 
       460        470        480        490        500
EEDHVQTVSL LREVQYNMGF LFAYSMRQKT RAYHRLKDDV PEEVKLRRLE 
       510        520        530        540        550
ELITIFREEA TKANQTSVGC TQLVLVEGLS KRSATDLCGR NDGNLKVIFP 
       560        570        580        590        600
DAEMEDVNNP GLRVRAQPGD YVLVKITSAS SQTLRGHVLC RTTLRDSSAY 

C
Note: May be due to intron retention.
Length:601
Mass (Da):67,689
Last modified:June 16, 2003 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i4AEBFD7DA5400902
GO
Isoform 2 (identifier: Q96SZ6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-90: Missing.
     293-306: Missing.

Note: Absence of the mitochondrial target sequence which may lead to miss-localization.1 Publication
Show »
Length:497
Mass (Da):56,223
Checksum:iC8FFBA14A5C3EB09
GO
Isoform 3 (identifier: Q96SZ6-3) [UniParc]FASTAAdd to basket
Also known as: CDK5RAP1_v11 Publication, isoform a

The sequence of this isoform differs from the canonical sequence as follows:
     293-306: Missing.

Show »
Length:587
Mass (Da):66,146
Checksum:i314E826B35C346EB
GO
Isoform 4 (identifier: Q96SZ6-4) [UniParc]FASTAAdd to basket
Also known as: isoform d

The sequence of this isoform differs from the canonical sequence as follows:
     293-383: Missing.

Show »
Length:510
Mass (Da):57,413
Checksum:i8F0DFB3B71E135BB
GO
Isoform 5 (identifier: Q96SZ6-5) [UniParc]FASTAAdd to basket
Also known as: CDK5RAP1_v41 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     292-305: Missing.
     435-440: GVSLSS → EDTGIS
     441-601: Missing.

Show »
Length:426
Mass (Da):48,072
Checksum:iD74ADCD179EA4FDC
GO
Isoform 6 (identifier: Q96SZ6-6) [UniParc]FASTAAdd to basket
Also known as: CDK5RAP1_v31 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     292-305: Missing.
     576-601: ITSASSQTLRGHVLCRTTLRDSSAYC → VRSPFGLLACTVK

Show »
Length:574
Mass (Da):64,709
Checksum:iA542DA7E2496A56B
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H0Y721H0Y721_HUMAN
CDK5 regulatory subunit-associated ...
CDK5RAP1
256Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

<p>This subsection of the 'Sequence' section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

The sequence AAD34147 differs from that shown. Reason: Frameshift.Curated
The sequence AAF29131 differs from that shown. Reason: Frameshift.Curated
The sequence BAB55120 differs from that shown. Reason: Erroneous termination. Truncated C-terminus.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti213R → Q in AAH01215 (PubMed:15489334).Curated1
Sequence conflicti217V → D (PubMed:11042152).Curated1
Sequence conflicti269F → L in BAB55120 (PubMed:14702039).Curated1
Sequence conflicti282I → T in BAB55120 (PubMed:14702039).Curated1
Sequence conflicti323D → G in BAB55120 (PubMed:14702039).Curated1
Sequence conflicti464V → A in BAB14760 (PubMed:14702039).Curated1
Sequence conflicti511T → S in AAF29131 (PubMed:11042152).Curated1
Sequence conflicti579Missing (PubMed:10810093).Curated1
Sequence conflicti585R → K in BAB14760 (PubMed:14702039).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_0075601 – 90Missing in isoform 2. 1 PublicationAdd BLAST90
Alternative sequenceiVSP_047799292 – 305Missing in isoform 5 and isoform 6. 1 PublicationAdd BLAST14
Alternative sequenceiVSP_007561293 – 383Missing in isoform 4. 1 PublicationAdd BLAST91
Alternative sequenceiVSP_007562293 – 306Missing in isoform 2 and isoform 3. 2 PublicationsAdd BLAST14
Alternative sequenceiVSP_047800435 – 440GVSLSS → EDTGIS in isoform 5. 1 Publication6
Alternative sequenceiVSP_047801441 – 601Missing in isoform 5. 1 PublicationAdd BLAST161
Alternative sequenceiVSP_047802576 – 601ITSAS…SSAYC → VRSPFGLLACTVK in isoform 6. 1 PublicationAdd BLAST26

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
AF152097 mRNA Translation: AAD34147.1 Frameshift.
AF161516 mRNA Translation: AAF29131.1 Frameshift.
AY462283 mRNA Translation: AAS18317.1
AY462284 mRNA Translation: AAS18318.1
AK023992 mRNA Translation: BAB14760.1
AK027449 mRNA Translation: BAB55120.1 Sequence problems.
AK292075 mRNA Translation: BAF84764.1
AL355392 Genomic DNA No translation available.
CH471077 Genomic DNA Translation: EAW76319.1
CH471077 Genomic DNA Translation: EAW76322.1
CH471077 Genomic DNA Translation: EAW76323.1
BC001215 mRNA Translation: AAH01215.1
BC050706 mRNA Translation: AAH50706.1

The Consensus CDS (CCDS) project

More...CCDSi		CCDS13219.1 [Q96SZ6-3]
CCDS63255.1 [Q96SZ6-4]

NCBI Reference Sequences

More...RefSeqi		NP_001265097.1, NM_001278168.1 [Q96SZ6-4]
NP_001265098.1, NM_001278169.1
NP_057166.4, NM_016082.4
NP_057492.2, NM_016408.3 [Q96SZ6-3]

Genome annotation databases

Ensembl eukaryotic genome annotation project

More...Ensembli		ENST00000339269; ENSP00000341840; ENSG00000101391 [Q96SZ6-4]
ENST00000346416; ENSP00000217372; ENSG00000101391 [Q96SZ6-3]
ENST00000357886; ENSP00000350558; ENSG00000101391 [Q96SZ6-1]
ENST00000473997; ENSP00000476857; ENSG00000101391 [Q96SZ6-2]

Database of genes from NCBI RefSeq genomes

More...GeneIDi		51654

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		hsa:51654

UCSC genome browser

More...UCSCi		uc002wyz.5 human [Q96SZ6-1]

Keywords - Coding sequence diversityi

Alternative splicing

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF152097 mRNA Translation: AAD34147.1 Frameshift.
AF161516 mRNA Translation: AAF29131.1 Frameshift.
AY462283 mRNA Translation: AAS18317.1
AY462284 mRNA Translation: AAS18318.1
AK023992 mRNA Translation: BAB14760.1
AK027449 mRNA Translation: BAB55120.1 Sequence problems.
AK292075 mRNA Translation: BAF84764.1
AL355392 Genomic DNA No translation available.
CH471077 Genomic DNA Translation: EAW76319.1
CH471077 Genomic DNA Translation: EAW76322.1
CH471077 Genomic DNA Translation: EAW76323.1
BC001215 mRNA Translation: AAH01215.1
BC050706 mRNA Translation: AAH50706.1
CCDSiCCDS13219.1 [Q96SZ6-3]
CCDS63255.1 [Q96SZ6-4]
RefSeqiNP_001265097.1, NM_001278168.1 [Q96SZ6-4]
NP_001265098.1, NM_001278169.1
NP_057166.4, NM_016082.4
NP_057492.2, NM_016408.3 [Q96SZ6-3]

3D structure databases

Database of comparative protein structure models

More...ModBasei		Search...

SWISS-MODEL Interactive Workspace

More...SWISS-MODEL-Workspacei		Submit a new modelling project...

Protein-protein interaction databases

BioGridi119661, 29 interactors
IntActiQ96SZ6, 17 interactors
STRINGi9606.ENSP00000217372

PTM databases

iPTMnetiQ96SZ6
PhosphoSitePlusiQ96SZ6

Polymorphism and mutation databases

BioMutaiCDK5RAP1
DMDMi32129446

Proteomic databases

EPDiQ96SZ6
jPOSTiQ96SZ6
MassIVEiQ96SZ6
MaxQBiQ96SZ6
PaxDbiQ96SZ6
PeptideAtlasiQ96SZ6
PRIDEiQ96SZ6
ProteomicsDBi65981
78165 [Q96SZ6-1]
78166 [Q96SZ6-2]
78167 [Q96SZ6-3]
78168 [Q96SZ6-4]

Protocols and materials databases

Antibodypedia a portal for validated antibodies

More...Antibodypediai		43007 166 antibodies

The DNASU plasmid repository

More...DNASUi		51654

Genome annotation databases

EnsembliENST00000339269; ENSP00000341840; ENSG00000101391 [Q96SZ6-4]
ENST00000346416; ENSP00000217372; ENSG00000101391 [Q96SZ6-3]
ENST00000357886; ENSP00000350558; ENSG00000101391 [Q96SZ6-1]
ENST00000473997; ENSP00000476857; ENSG00000101391 [Q96SZ6-2]
GeneIDi51654
KEGGihsa:51654
UCSCiuc002wyz.5 human [Q96SZ6-1]

Organism-specific databases

Comparative Toxicogenomics Database

More...CTDi		51654
DisGeNETi51654

GeneCards: human genes, protein and diseases

More...GeneCardsi		CDK5RAP1
HGNCiHGNC:15880 CDK5RAP1
HPAiENSG00000101391 Low tissue specificity
MIMi608200 gene
neXtProtiNX_Q96SZ6
OpenTargetsiENSG00000101391
PharmGKBiPA26313

GenAtlas: human gene database

More...GenAtlasi		Search...

Phylogenomic databases

eggNOGiKOG2492 Eukaryota
COG0621 LUCA
GeneTreeiENSGT00940000160361
HOGENOMiCLU_018697_2_1_1
InParanoidiQ96SZ6
OMAiCEHFHIP
OrthoDBi835984at2759
PhylomeDBiQ96SZ6
TreeFamiTF101033

Miscellaneous databases

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

More...ChiTaRSi		CDK5RAP1 human

The Gene Wiki collection of pages on human genes and proteins

More...GeneWikii		CDK5RAP1

Database of phenotypes from RNA interference screens in Drosophila and Homo sapiens

More...GenomeRNAii		51654
PharosiQ96SZ6 Tbio

Protein Ontology

More...PROi		PR:Q96SZ6
RNActiQ96SZ6 protein

The Stanford Online Universal Resource for Clones and ESTs

More...SOURCEi		Search...

Gene expression databases

BgeeiENSG00000101391 Expressed in small intestine Peyer's patch and 225 other tissues
ExpressionAtlasiQ96SZ6 baseline and differential
GenevisibleiQ96SZ6 HS

Family and domain databases

Gene3Di3.40.50.12160, 1 hit
3.80.30.20, 1 hit
HAMAPiMF_01864 tRNA_metthiotr_MiaB, 1 hit
InterProiView protein in InterPro
IPR006638 Elp3/MiaB/NifB
IPR005839 Methylthiotransferase
IPR020612 Methylthiotransferase_CS
IPR013848 Methylthiotransferase_N
IPR038135 Methylthiotransferase_N_sf
IPR006463 MiaB_methiolase
IPR007197 rSAM
IPR023404 rSAM_horseshoe
IPR002792 TRAM_dom
PfamiView protein in Pfam
PF04055 Radical_SAM, 1 hit
PF01938 TRAM, 1 hit
PF00919 UPF0004, 1 hit
SFLDiSFLDF00273 (dimethylallyl)adenosine_tRNA, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00089 TIGR00089, 1 hit
PROSITEiView protein in PROSITE
PS51449 MTTASE_N, 1 hit
PS01278 MTTASE_RADICAL, 1 hit
PS50926 TRAM, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...ProtoNeti		Search...

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiCK5P1_HUMAN
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q96SZ6
Secondary accession number(s): A8K7R0
, Q5QP46, Q5QP47, Q5QP48, Q675N4, Q675N5, Q9BVG6, Q9BWZ5, Q9H859, Q9NZZ9, Q9Y3F0
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: June 16, 2003
Last modified: April 22, 2020
This is version 172 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
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