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Protein

Protein IWS1 homolog

Gene

IWS1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription factor which plays a key role in defining the composition of the RNA polymerase II (RNAPII) elongation complex and in modulating the production of mature mRNA transcripts. Acts as an assembly factor to recruit various factors to the RNAPII elongation complex and is recruited to the complex via binding to the transcription elongation factor SUPT6H bound to the C-terminal domain (CTD) of the RNAPII subunit RPB1 (POLR2A). The SUPT6H:IWS1:CTD complex recruits mRNA export factors (ALYREF/THOC4, EXOSC10) as well as histone modifying enzymes (such as SETD2) to ensure proper mRNA splicing, efficient mRNA export and elongation-coupled H3K36 methylation, a signature chromatin mark of active transcription.3 Publications

GO - Biological processi

Keywordsi

Biological processmRNA processing, mRNA splicing, mRNA transport, Transcription, Transcription regulation, Transport

Enzyme and pathway databases

ReactomeiR-HSA-112382 Formation of RNA Pol II elongation complex
R-HSA-674695 RNA Polymerase II Pre-transcription Events
R-HSA-75955 RNA Polymerase II Transcription Elongation

Names & Taxonomyi

Protein namesi
Recommended name:
Protein IWS1 homolog
Alternative name(s):
IWS1-like protein
Gene namesi
Name:IWS1
Synonyms:IWS1L
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

EuPathDBiHostDB:ENSG00000163166.13
HGNCiHGNC:25467 IWS1
neXtProtiNX_Q96ST2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000163166
PharmGKBiPA144596419

Polymorphism and mutation databases

BioMutaiIWS1
DMDMi85542184

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000833461 – 819Protein IWS1 homologAdd BLAST819

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei27PhosphoserineBy similarity1
Modified residuei54PhosphoserineCombined sources1
Modified residuei69PhosphoserineCombined sources1
Modified residuei157PhosphoserineCombined sources1
Modified residuei159PhosphoserineCombined sources1
Modified residuei183PhosphoserineCombined sources1
Modified residuei196PhosphoserineCombined sources1
Modified residuei198PhosphoserineCombined sources1
Modified residuei209PhosphoserineBy similarity1
Modified residuei235PhosphoserineCombined sources1
Modified residuei237PhosphoserineCombined sources1
Modified residuei248PhosphoserineCombined sources1
Modified residuei250PhosphoserineCombined sources1
Modified residuei261PhosphoserineCombined sources1
Modified residuei263PhosphoserineCombined sources1
Modified residuei274PhosphoserineCombined sources1
Modified residuei276PhosphoserineCombined sources1
Modified residuei287PhosphoserineCombined sources1
Modified residuei289PhosphoserineCombined sources1
Modified residuei300PhosphoserineCombined sources1
Modified residuei302PhosphoserineCombined sources1
Modified residuei304PhosphoserineCombined sources1
Modified residuei313PhosphoserineCombined sources1
Modified residuei315PhosphoserineCombined sources1
Modified residuei329PhosphoserineCombined sources1
Modified residuei333PhosphoserineCombined sources1
Modified residuei351PhosphoserineBy similarity1
Modified residuei362PhosphoserineBy similarity1
Modified residuei363PhosphoserineCombined sources1
Modified residuei365PhosphoserineCombined sources1
Modified residuei377PhosphoserineCombined sources1
Modified residuei398PhosphoserineCombined sources1
Modified residuei400PhosphoserineCombined sources1
Modified residuei415PhosphoserineCombined sources1
Modified residuei420PhosphoserineCombined sources1
Modified residuei422PhosphoserineCombined sources1
Modified residuei426PhosphoserineBy similarity1
Modified residuei435PhosphothreonineCombined sources1
Modified residuei438PhosphoserineCombined sources1
Modified residuei440PhosphoserineCombined sources1
Modified residuei480PhosphoserineCombined sources1
Modified residuei511PhosphoserineCombined sources1
Modified residuei513PhosphoserineCombined sources1
Modified residuei725PhosphothreonineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ96ST2
MaxQBiQ96ST2
PaxDbiQ96ST2
PeptideAtlasiQ96ST2
PRIDEiQ96ST2
ProteomicsDBi78143
78144 [Q96ST2-2]
78145 [Q96ST2-3]

PTM databases

iPTMnetiQ96ST2
PhosphoSitePlusiQ96ST2

Expressioni

Gene expression databases

BgeeiENSG00000163166 Expressed in 202 organ(s), highest expression level in corpus callosum
CleanExiHS_IWS1
ExpressionAtlasiQ96ST2 baseline and differential
GenevisibleiQ96ST2 HS

Organism-specific databases

HPAiHPA035719
HPA061866

Interactioni

Subunit structurei

Interacts with SUPT6H; binds preferentially to the POLR2A-bound SUPT6H. Interacts with ALYREF/THOC4, SETD2 and PRMT5. Interacts with HDGFRP2.4 Publications

Protein-protein interaction databases

BioGridi120807, 38 interactors
IntActiQ96ST2, 22 interactors
MINTiQ96ST2
STRINGi9606.ENSP00000295321

Structurei

3D structure databases

ProteinModelPortaliQ96ST2
SMRiQ96ST2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati235 – 2601Add BLAST26
Repeati261 – 2862Add BLAST26
Repeati287 – 2963; half-length10
Domaini614 – 692TFIIS N-terminalPROSITE-ProRule annotationAdd BLAST79

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni235 – 2963 X approximate tandem repeatsAdd BLAST62
Regioni523 – 819Interaction with SUPT6H and ALYREF1 PublicationAdd BLAST297

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi83 – 509Glu-richAdd BLAST427

Sequence similaritiesi

Belongs to the IWS1 family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1793 Eukaryota
COG5139 LUCA
GeneTreeiENSGT00720000108834
HOVERGENiHBG081811
InParanoidiQ96ST2
KOiK17498
OMAiRHQASDS
OrthoDBiEOG091G0K1Z
PhylomeDBiQ96ST2
TreeFamiTF315504

Family and domain databases

Gene3Di1.20.930.10, 1 hit
InterProiView protein in InterPro
IPR035441 TFIIS/LEDGF_dom_sf
IPR017923 TFIIS_N
PfamiView protein in Pfam
PF08711 Med26, 1 hit
SUPFAMiSSF47676 SSF47676, 1 hit
PROSITEiView protein in PROSITE
PS51319 TFIIS_N, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 3 potential isoforms that are computationally mapped.iShow all

Isoform 1 (identifier: Q96ST2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MDSEYYSGDQ SDDGGATPVQ DERDSGSDGE DDVNEQHSGS DTGSVERHSE
60 70 80 90 100
NETSDREDGL PKGHHVTDSE NDEPLNLNAS DSESEELHRQ KDSDSESEER
110 120 130 140 150
AEPPASDSEN EDVNQHGSDS ESEETRKLPG SDSENEELLN GHASDSENED
160 170 180 190 200
VGKHPASDSE IEELQKSPAS DSETEDALKP QISDSESEEP PRHQASDSEN
210 220 230 240 250
EEPPKPRMSD SESEELPKPQ VSDSESEEPP RHQASDSENE ELPKPRISDS
260 270 280 290 300
ESEDPPRHQA SDSENEELPK PRISDSESED PPRNQASDSE NEELPKPRVS
310 320 330 340 350
DSESEGPQKG PASDSETEDA SRHKQKPESD DDSDRENKGE DTEMQNDSFH
360 370 380 390 400
SDSHMDRKKF HSSDSEEEEH KKQKMDSDED EKEGEEEKVA KRKAAVLSDS
410 420 430 440 450
EDEEKASAKK SRVVSDADDS DSDAVSDKSG KREKTIASDS EEEAGKELSD
460 470 480 490 500
KKNEEKDLFG SDSESGNEEE NLIADIFGES GDEEEEEFTG FNQEDLEEEK
510 520 530 540 550
GETQVKEAED SDSDDNIKRG KHMDFLSDFE MMLQRKKSMS GKRRRNRDGG
560 570 580 590 600
TFISDADDVV SAMIVKMNEA AEEDRQLNNQ KKPALKKLTL LPAVVMHLKK
610 620 630 640 650
QDLKETFIDS GVMSAIKEWL SPLPDRSLPA LKIREELLKI LQELPSVSQE
660 670 680 690 700
TLKHSGIGRA VMYLYKHPKE SRSNKDMAGK LINEWSRPIF GLTSNYKGMT
710 720 730 740 750
REEREQRDLE QMPQRRRMNS TGGQTPRRDL EKVLTGEEKA LRPGDPGFCA
760 770 780 790 800
RARVPMPSNK DYVVRPKWNV EMESSRFQAT SKKGISRLDK QMRKFTDIRK
810
KSRSAHAVKI SIEGNKMPL
Length:819
Mass (Da):91,955
Last modified:January 10, 2006 - v2
Checksum:iC9749A2C2DC05D72
GO
Isoform 2 (identifier: Q96ST2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     20-344: Missing.

Note: No experimental confirmation available.
Show »
Length:494
Mass (Da):56,109
Checksum:i350E71E75B0B06DE
GO
Isoform 3 (identifier: Q96ST2-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     54-260: Missing.

Note: No experimental confirmation available.
Show »
Length:612
Mass (Da):69,108
Checksum:i6A011627CCFAE58B
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A1B0GW95A0A1B0GW95_HUMAN
Protein IWS1 homolog
IWS1
207Annotation score:
B8ZZB6B8ZZB6_HUMAN
Protein IWS1 homolog
IWS1
292Annotation score:
H7C0U1H7C0U1_HUMAN
Protein IWS1 homolog
IWS1
33Annotation score:

Sequence cautioni

The sequence AAH65279 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated
The sequence BAA91858 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti244K → R in BAB55198 (PubMed:14702039).Curated1
Sequence conflicti530E → G in BAA91402 (PubMed:14702039).Curated1
Sequence conflicti559V → D in BAB55198 (PubMed:14702039).Curated1
Sequence conflicti786S → G in BAB55198 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_055975390A → V. Corresponds to variant dbSNP:rs34377117Ensembl.1
Natural variantiVAR_055976425V → I. Corresponds to variant dbSNP:rs34785867Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_01699220 – 344Missing in isoform 2. 1 PublicationAdd BLAST325
Alternative sequenceiVSP_01699354 – 260Missing in isoform 3. 1 PublicationAdd BLAST207

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK000868 mRNA Translation: BAA91402.1
AK001717 mRNA Translation: BAA91858.1 Different initiation.
AK027561 mRNA Translation: BAB55198.1
AK056881 mRNA Translation: BAB71301.1
AL834178 mRNA Translation: CAD38875.1
BC065279 mRNA Translation: AAH65279.1 Sequence problems.
BC110536 mRNA Translation: AAI10537.1
BC110537 mRNA Translation: AAI10538.1
CCDSiCCDS2146.1 [Q96ST2-1]
RefSeqiNP_060439.2, NM_017969.2 [Q96ST2-1]
UniGeneiHs.469879
Hs.679491

Genome annotation databases

EnsembliENST00000295321; ENSP00000295321; ENSG00000163166 [Q96ST2-1]
GeneIDi55677
KEGGihsa:55677
UCSCiuc002ton.3 human [Q96ST2-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiIWS1_HUMAN
AccessioniPrimary (citable) accession number: Q96ST2
Secondary accession number(s): Q2TB65
, Q6P157, Q8N3E8, Q96MI7, Q9NV97, Q9NWH8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: January 10, 2006
Last modified: September 12, 2018
This is version 138 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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