UniProtKB - Q96SQ9 (CP2S1_HUMAN)
Protein
Cytochrome P450 2S1
Gene
CYP2S1
Organism
Homo sapiens (Human)
Status
Functioni
A cytochrome P450 monooxygenase involved in the metabolism of retinoids and eicosanoids (PubMed:12711469, PubMed:21068195). In epidermis, may contribute to the oxidative metabolism of all-trans-retinoic acid. For this activity, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase) (PubMed:12711469). Additionally, displays peroxidase and isomerase activities toward various oxygenated eicosanoids such as prostaglandin H2 (PGH2) and hydroperoxyeicosatetraenoates (HPETEs) (PubMed:21068195). Independently of cytochrome P450 reductase, NADPH, and O2, catalyzes the breakdown of PGH2 to hydroxyheptadecatrienoic acid (HHT) and malondialdehyde (MDA), which is known to act as a mediator of DNA damage (PubMed:21068195).2 Publications
Catalytic activityi
- all-trans-retinoate + O2 + reduced [NADPH—hemoprotein reductase] = all-trans-5,6-epoxyretinoate + H+ + H2O + oxidized [NADPH—hemoprotein reductase]1 PublicationThis reaction proceeds in the forward1 Publication direction.
- all-trans-retinoate + O2 + reduced [NADPH—hemoprotein reductase] = all-trans-4-hydroxyretinoate + H+ + H2O + oxidized [NADPH—hemoprotein reductase]1 PublicationThis reaction proceeds in the forward1 Publication direction.
- (5S)-hydroperoxy-(6E,8Z,11Z,14Z)-eicosatetraenoate = 5-oxo-(6E,8Z,11Z,14Z)-eicosatetraenoate + H2O1 PublicationThis reaction proceeds in the forward1 Publication direction.
- (12S)-hydroperoxy-(5Z,8Z,10E,14Z)-eicosatetraenoate = 12-oxo-(5Z,8Z,10E,14Z)-eicosatetraenoate + H2O1 PublicationEC:4.2.1.1521 PublicationThis reaction proceeds in the forward1 Publication direction.
- (15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoate = 15-oxo-(5Z,8Z,11Z,13E)-eicosatetraenoate + H2O1 PublicationThis reaction proceeds in the forward1 Publication direction.
- EC:5.3.99.51 PublicationThis reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
Cofactori
hemeBy similarity
: fatty acid metabolism Pathwayi
This protein is involved in the pathway fatty acid metabolism, which is part of Lipid metabolism.1 PublicationView all proteins of this organism that are known to be involved in the pathway fatty acid metabolism and in Lipid metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 440 | Iron (heme axial ligand)By similarity | 1 |
GO - Molecular functioni
- arachidonic acid epoxygenase activity Source: GO_Central
- heme binding Source: GO_Central
- hydro-lyase activity Source: UniProtKB
- hydroperoxy icosatetraenoate dehydratase activity Source: UniProtKB-EC
- iron ion binding Source: InterPro
- monooxygenase activity Source: UniProtKB
- oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen Source: GO_Central
- retinoic acid 4-hydroxylase activity Source: Reactome
- steroid hydroxylase activity Source: GO_Central
- thromboxane-A synthase activity Source: UniProtKB
GO - Biological processi
- epoxygenase P450 pathway Source: GO_Central
- exogenous drug catabolic process Source: GO_Central
- icosanoid metabolic process Source: UniProtKB
- organic acid metabolic process Source: GO_Central
- oxidation-reduction process Source: GO_Central
- prostaglandin metabolic process Source: UniProtKB
- retinoic acid metabolic process Source: UniProtKB
- xenobiotic metabolic process Source: GO_Central
Keywordsi
Molecular function | Isomerase, Lyase, Monooxygenase, Oxidoreductase |
Biological process | Fatty acid metabolism, Lipid metabolism |
Ligand | Heme, Iron, Metal-binding |
Enzyme and pathway databases
PathwayCommonsi | Q96SQ9 |
Reactomei | R-HSA-211958, Miscellaneous substrates R-HSA-211981, Xenobiotics R-HSA-211999, CYP2E1 reactions |
UniPathwayi | UPA00199 |
Chemistry databases
SwissLipidsi | SLP:000001465 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | ORF Names:UNQ891/PRO1906 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000167600.13 |
HGNCi | HGNC:15654, CYP2S1 |
MIMi | 611529, gene |
neXtProti | NX_Q96SQ9 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane 1 Publication; Peripheral membrane protein 1 Publication
- Microsome membrane 1 Publication; Peripheral membrane protein 1 Publication
Endoplasmic reticulum
- endoplasmic reticulum Source: HPA
- endoplasmic reticulum membrane Source: Reactome
Other locations
- cytoplasm Source: GO_Central
- intracellular membrane-bounded organelle Source: GO_Central
Keywords - Cellular componenti
Endoplasmic reticulum, Membrane, MicrosomePathology & Biotechi
Organism-specific databases
DisGeNETi | 29785 |
OpenTargetsi | ENSG00000167600 |
PharmGKBi | PA27113 |
Miscellaneous databases
Pharosi | Q96SQ9, Tbio |
Polymorphism and mutation databases
BioMutai | CYP2S1 |
DMDMi | 48428134 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000051780 | 1 – 504 | Cytochrome P450 2S1Add BLAST | 504 |
Proteomic databases
EPDi | Q96SQ9 |
jPOSTi | Q96SQ9 |
MassIVEi | Q96SQ9 |
MaxQBi | Q96SQ9 |
PaxDbi | Q96SQ9 |
PeptideAtlasi | Q96SQ9 |
PRIDEi | Q96SQ9 |
ProteomicsDBi | 78138 [Q96SQ9-1] 78139 [Q96SQ9-2] |
PTM databases
iPTMneti | Q96SQ9 |
PhosphoSitePlusi | Q96SQ9 |
Expressioni
Tissue specificityi
Expressed at higher levels in extrahepatic tissues including trachea, lung, stomach, small intestine, colon, kidney, breast, placenta and spleen (PubMed:11181079, PubMed:12711469). Expressed in peripheral blood leukocytes (PubMed:11181079). Constitutively expressed in skin (at protein level) (PubMed:12711469).2 Publications
Inductioni
Up-regulated in skin upon exposure to ultraviolet radiation or treatment with all-trans retinoic acid (substrate-inducible).1 Publication
Gene expression databases
Bgeei | ENSG00000167600, Expressed in stomach and 135 other tissues |
ExpressionAtlasi | Q96SQ9, baseline and differential |
Genevisiblei | Q96SQ9, HS |
Organism-specific databases
HPAi | ENSG00000167600, Tissue enhanced (blood, gallbladder, intestine, stomach) |
Interactioni
Binary interactionsi
Hide detailsQ96SQ9
With | #Exp. | IntAct |
---|---|---|
ADAMTSL4 - isoform 3 [Q6UY14-3] | 3 | EBI-8633740,EBI-10173507 |
KRTAP10-8 [P60410] | 3 | EBI-8633740,EBI-10171774 |
Protein-protein interaction databases
BioGRIDi | 118915, 41 interactors |
IntActi | Q96SQ9, 39 interactors |
MINTi | Q96SQ9 |
STRINGi | 9606.ENSP00000308032 |
Miscellaneous databases
RNActi | Q96SQ9, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the cytochrome P450 family.Curated
Phylogenomic databases
eggNOGi | KOG0156, Eukaryota |
GeneTreei | ENSGT00940000162131 |
HOGENOMi | CLU_001570_22_3_1 |
InParanoidi | Q96SQ9 |
OMAi | MFSWFLR |
OrthoDBi | 702827at2759 |
PhylomeDBi | Q96SQ9 |
TreeFami | TF352043 |
Family and domain databases
Gene3Di | 1.10.630.10, 1 hit |
InterProi | View protein in InterPro IPR001128, Cyt_P450 IPR017972, Cyt_P450_CS IPR002401, Cyt_P450_E_grp-I IPR008067, Cyt_P450_E_grp-I_CYP2A-like IPR036396, Cyt_P450_sf |
Pfami | View protein in Pfam PF00067, p450, 1 hit |
PRINTSi | PR00463, EP450I PR01684, EP450ICYP2A PR00385, P450 |
SUPFAMi | SSF48264, SSF48264, 1 hit |
PROSITEi | View protein in PROSITE PS00086, CYTOCHROME_P450, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q96SQ9-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MEATGTWALL LALALLLLLT LALSGTRARG HLPPGPTPLP LLGNLLQLRP
60 70 80 90 100
GALYSGLMRL SKKYGPVFTI YLGPWRPVVV LVGQEAVREA LGGQAEEFSG
110 120 130 140 150
RGTVAMLEGT FDGHGVFFSN GERWRQLRKF TMLALRDLGM GKREGEELIQ
160 170 180 190 200
AEARCLVETF QGTEGRPFDP SLLLAQATSN VVCSLLFGLR FSYEDKEFQA
210 220 230 240 250
VVRAAGGTLL GVSSQGGQTY EMFSWFLRPL PGPHKQLLHH VSTLAAFTVR
260 270 280 290 300
QVQQHQGNLD ASGPARDLVD AFLLKMAQEE QNPGTEFTNK NMLMTVIYLL
310 320 330 340 350
FAGTMTVSTT VGYTLLLLMK YPHVQKWVRE ELNRELGAGQ APSLGDRTRL
360 370 380 390 400
PYTDAVLHEA QRLLALVPMG IPRTLMRTTR FRGYTLPQGT EVFPLLGSIL
410 420 430 440 450
HDPNIFKHPE EFNPDRFLDA DGRFRKHEAF LPFSLGKRVC LGEGLAKAEL
460 470 480 490 500
FLFFTTILQA FSLESPCPPD TLSLKPTVSG LFNIPPAFQL QVRPTDLHST
TQTR
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketM0R057 | M0R057_HUMAN | Cytochrome P450 2S1 | CYP2S1 | 187 | Annotation score: | ||
M0R152 | M0R152_HUMAN | Cytochrome P450 2S1 | CYP2S1 | 175 | Annotation score: | ||
M0R2G8 | M0R2G8_HUMAN | Cytochrome P450 2S1 | CYP2S1 | 165 | Annotation score: | ||
M0QYI2 | M0QYI2_HUMAN | Cytochrome P450 2S1 | CYP2S1 | 73 | Annotation score: | ||
M0QY51 | M0QY51_HUMAN | Cytochrome P450 2S1 | CYP2S1 | 60 | Annotation score: |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_033820 | 466 | P → L. Corresponds to variant dbSNP:rs34971233Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_010531 | 488 – 504 | FQLQV…TTQTR → STVGMDRVNVSRVYTAGSHI YTPAVVFRSLSHGPHAHLTH AAKMHNRTPIHNYKGHKATA GLAFHRHKYSPSAITST in isoform 2. 1 PublicationAdd BLAST | 17 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF335278 mRNA Translation: AAK13498.1 AY358603 mRNA Translation: AAQ88966.1 AK027605 mRNA Translation: BAB55227.1 BC033691 mRNA Translation: AAH33691.1 |
CCDSi | CCDS12573.1 [Q96SQ9-1] |
PIRi | JC7613 |
RefSeqi | NP_085125.1, NM_030622.7 [Q96SQ9-1] |
Genome annotation databases
Ensembli | ENST00000310054; ENSP00000308032; ENSG00000167600 [Q96SQ9-1] |
GeneIDi | 29785 |
KEGGi | hsa:29785 |
UCSCi | uc002opw.4, human [Q96SQ9-1] |
Keywords - Coding sequence diversityi
Alternative splicing, PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF335278 mRNA Translation: AAK13498.1 AY358603 mRNA Translation: AAQ88966.1 AK027605 mRNA Translation: BAB55227.1 BC033691 mRNA Translation: AAH33691.1 |
CCDSi | CCDS12573.1 [Q96SQ9-1] |
PIRi | JC7613 |
RefSeqi | NP_085125.1, NM_030622.7 [Q96SQ9-1] |
3D structure databases
SMRi | Q96SQ9 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 118915, 41 interactors |
IntActi | Q96SQ9, 39 interactors |
MINTi | Q96SQ9 |
STRINGi | 9606.ENSP00000308032 |
Chemistry databases
SwissLipidsi | SLP:000001465 |
PTM databases
iPTMneti | Q96SQ9 |
PhosphoSitePlusi | Q96SQ9 |
Polymorphism and mutation databases
BioMutai | CYP2S1 |
DMDMi | 48428134 |
Proteomic databases
EPDi | Q96SQ9 |
jPOSTi | Q96SQ9 |
MassIVEi | Q96SQ9 |
MaxQBi | Q96SQ9 |
PaxDbi | Q96SQ9 |
PeptideAtlasi | Q96SQ9 |
PRIDEi | Q96SQ9 |
ProteomicsDBi | 78138 [Q96SQ9-1] 78139 [Q96SQ9-2] |
Protocols and materials databases
Antibodypediai | 45241, 202 antibodies |
DNASUi | 29785 |
Genome annotation databases
Ensembli | ENST00000310054; ENSP00000308032; ENSG00000167600 [Q96SQ9-1] |
GeneIDi | 29785 |
KEGGi | hsa:29785 |
UCSCi | uc002opw.4, human [Q96SQ9-1] |
Organism-specific databases
CTDi | 29785 |
DisGeNETi | 29785 |
EuPathDBi | HostDB:ENSG00000167600.13 |
GeneCardsi | CYP2S1 |
HGNCi | HGNC:15654, CYP2S1 |
HPAi | ENSG00000167600, Tissue enhanced (blood, gallbladder, intestine, stomach) |
MIMi | 611529, gene |
neXtProti | NX_Q96SQ9 |
OpenTargetsi | ENSG00000167600 |
PharmGKBi | PA27113 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0156, Eukaryota |
GeneTreei | ENSGT00940000162131 |
HOGENOMi | CLU_001570_22_3_1 |
InParanoidi | Q96SQ9 |
OMAi | MFSWFLR |
OrthoDBi | 702827at2759 |
PhylomeDBi | Q96SQ9 |
TreeFami | TF352043 |
Enzyme and pathway databases
UniPathwayi | UPA00199 |
PathwayCommonsi | Q96SQ9 |
Reactomei | R-HSA-211958, Miscellaneous substrates R-HSA-211981, Xenobiotics R-HSA-211999, CYP2E1 reactions |
Miscellaneous databases
BioGRID-ORCSi | 29785, 6 hits in 846 CRISPR screens |
ChiTaRSi | CYP2S1, human |
GeneWikii | CYP2S1 |
GenomeRNAii | 29785 |
Pharosi | Q96SQ9, Tbio |
PROi | PR:Q96SQ9 |
RNActi | Q96SQ9, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000167600, Expressed in stomach and 135 other tissues |
ExpressionAtlasi | Q96SQ9, baseline and differential |
Genevisiblei | Q96SQ9, HS |
Family and domain databases
Gene3Di | 1.10.630.10, 1 hit |
InterProi | View protein in InterPro IPR001128, Cyt_P450 IPR017972, Cyt_P450_CS IPR002401, Cyt_P450_E_grp-I IPR008067, Cyt_P450_E_grp-I_CYP2A-like IPR036396, Cyt_P450_sf |
Pfami | View protein in Pfam PF00067, p450, 1 hit |
PRINTSi | PR00463, EP450I PR01684, EP450ICYP2A PR00385, P450 |
SUPFAMi | SSF48264, SSF48264, 1 hit |
PROSITEi | View protein in PROSITE PS00086, CYTOCHROME_P450, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CP2S1_HUMAN | |
Accessioni | Q96SQ9Primary (citable) accession number: Q96SQ9 Secondary accession number(s): Q9BZ66 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 7, 2004 |
Last sequence update: | June 7, 2004 | |
Last modified: | December 2, 2020 | |
This is version 163 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families - Human chromosome 19
Human chromosome 19: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations